Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	151	87.4	2971636	99.2%	2947862	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,984,869	T→C	E380G (GAA→GGA)	aslA ←	arylsulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,984,869	0	T	C	100.0%	343.6 / NA	96	E380G (GAA→GGA)	aslA	arylsulfatase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (45/51); total (45/51)

TTTCGCTCCAGGATGCCCTGCCAGATCCAGCGCGGTAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTTCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCAGTGTTTTATACAGA  >  NC_000913/3984739‑3985003

tttCGCTCCAGGATGCCCTGCCAGATCCAGCGCGGTAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCg                                                                                                                               >  2:471847/1‑140 (MQ=255)

         aGGATGCCCTGCCAGATCCATCGCGGTAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCg                                                                                                                      >  2:266826/1‑140 (MQ=255)

          ggATGCCCTGCCAGATCCAGCGCGGTAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGc                                                                                                                     >  1:717015/1‑140 (MQ=255)

           gATGCCCTGCCAGATCCAGCGCGGTAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGca                                                                                                                    >  1:831540/1‑140 (MQ=255)

             tGCCCTGCCAGATCCAGCGCGGTAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGcacc                                                                                                                  <  2:1382674/140‑1 (MQ=255)

               cccTGCCAGATCCAGCGCGGTAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGcaccac                                                                                                                >  1:1382058/1‑140 (MQ=255)

               cccTGCCAGATCCAGCGCGGTAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGcaccac                                                                                                                <  1:186384/140‑1 (MQ=255)

               cccTGCCAGATCCAGCGCGGTAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGcaccac                                                                                                                <  1:1200488/140‑1 (MQ=255)

                ccTGTCAGATCCAGCGCGGTAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACg                                                                                                               >  2:479808/1‑140 (MQ=255)

                        aTCCAGCGCGGTAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACgggg                                                                                                       >  1:172727/1‑140 (MQ=255)

                             gcgcGGTAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGt                                                                                                  >  2:1124313/1‑140 (MQ=255)

                             gcgcGGTAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGt                                                                                                  >  2:69524/1‑140 (MQ=255)

                                   tAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTgc                                                                                            >  2:883312/1‑140 (MQ=255)

                                   tAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTgc                                                                                            >  1:772461/1‑140 (MQ=255)

                                     gggAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCGGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTgcgg                                                                                          >  1:869833/1‑140 (MQ=255)

                                     gggAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTgcgg                                                                                          <  1:1396739/140‑1 (MQ=255)

                                          gagaTCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTa                                                                                     <  1:1124313/140‑1 (MQ=255)

                                          gagaTCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTa                                                                                     <  1:69524/140‑1 (MQ=255)

                                           agaTCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTAc                                                                                    >  2:1243210/1‑140 (MQ=255)

                                             aTCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACtt                                                                                  >  1:620239/1‑140 (MQ=255)

                                             aTCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACtt                                                                                  >  1:292523/1‑140 (MQ=255)

                                             aTCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACtt                                                                                  >  2:1044005/1‑140 (MQ=255)

                                             aTCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACtt                                                                                  >  2:171734/1‑140 (MQ=255)

                                             aTCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACtt                                                                                  >  2:439653/1‑140 (MQ=255)

                                             aTCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACtt                                                                                  >  1:11483/1‑140 (MQ=255)

                                             aTCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCAGCGGTACtt                                                                                  >  1:415187/1‑140 (MQ=255)

                                               cTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCg                                                                                >  2:1170250/1‑140 (MQ=255)

                                               cTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCg                                                                                >  2:313427/1‑140 (MQ=255)

                                                tGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCgg                                                                               >  1:1318634/1‑140 (MQ=255)

                                                 gCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGc                                                                              >  1:419138/1‑140 (MQ=255)

                                                 gCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGc                                                                              <  1:471847/140‑1 (MQ=255)

                                                    aGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTc                                                                           <  1:671776/140‑1 (MQ=255)

                                                    aGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTc                                                                           <  1:500713/140‑1 (MQ=255)

                                                     gATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTcc                                                                          >  1:1088260/1‑140 (MQ=255)

                                                      aTCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGTGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCg                                                                         <  2:609182/140‑1 (MQ=255)

                                                       tCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCgg                                                                        <  1:266826/140‑1 (MQ=255)

                                                           cAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCg                                                                    <  2:499042/140‑1 (MQ=255)

                                                           cAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCg                                                                    <  2:172727/140‑1 (MQ=255)

                                                            aaTACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGt                                                                   >  2:271533/1‑140 (MQ=255)

                                                               aCCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTAt                                                                <  1:1442947/140‑1 (MQ=255)

                                                               aCCGTCAGATTTACGCGGTTGGATCATCCCTTACCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTAt                                                                <  2:349302/140‑1 (MQ=255)

                                                                 cGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCg                                                              <  2:700706/140‑1 (MQ=255)

                                                                 cGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCg                                                              <  1:883312/140‑1 (MQ=255)

                                                                 cGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCg                                                              >  1:800161/1‑140 (MQ=255)

                                                                 cGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCg                                                              >  2:615243/1‑140 (MQ=255)

                                                                  gTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCgg                                                             <  2:831540/140‑1 (MQ=255)

                                                                   tCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGa                                                            >  2:256230/1‑140 (MQ=255)

                                                                   tCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGa                                                            >  2:655147/1‑140 (MQ=255)

                                                                   tCAGATTTACGCGGTTGGATCATCCCTTT‑‑AGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAgg                                                          >  1:859797/1‑140 (MQ=255)

                                                                     aGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAgg                                                          >  1:714406/1‑140 (MQ=255)

                                                                     aGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAgg                                                          <  2:772461/140‑1 (MQ=255)

                                                                     aGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAgg                                                          >  1:1343253/1‑140 (MQ=255)

                                                                           aCGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGa                                                    <  1:313427/140‑1 (MQ=255)

                                                                               ggTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGAt                                                <  2:1343253/140‑1 (MQ=255)

                                                                               ggTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGAt                                                <  1:1170250/140‑1 (MQ=255)

                                                                                 ttGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGAt                                                <  2:612406/138‑1 (MQ=255)

                                                                                 ttGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGAt                                                >  1:612406/1‑138 (MQ=255)

                                                                                 ttGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCa                                              <  1:1243210/140‑1 (MQ=255)

                                                                                 ttGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCa                                              <  1:1044005/140‑1 (MQ=255)

                                                                                   ggATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAGCGCCGCCTCCCCAGGTCGAACCTGTCGCACCACGCAACGGGGTGCGTCCGTGCGCCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGc                                            <  2:1351659/140‑1 (MQ=255)

                                                                                   ggATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGc                                            >  2:248950/1‑140 (MQ=255)

                                                                                    gATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCg                                           <  1:164237/140‑1 (MQ=255)

                                                                                    gATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCg                                           <  1:615243/140‑1 (MQ=255)

                                                                                     atcatcCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCgt                                          <  1:248950/140‑1 (MQ=255)

                                                                                      tcatcCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCgtg                                         <  2:717015/140‑1 (MQ=255)

                                                                                         tcCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTa                                      >  1:452558/1‑140 (MQ=255)

                                                                                         tcCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTa                                      >  1:1394217/1‑140 (MQ=255)

                                                                                           ccTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATc                                    >  1:22702/1‑140 (MQ=255)

                                                                                              ttCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAg                                 <  2:452558/140‑1 (MQ=255)

                                                                                               tCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGc                                >  2:313359/1‑140 (MQ=255)

                                                                                               tCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGc                                >  1:37062/1‑140 (MQ=255)

                                                                                                   gTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGAc                            <  1:655147/140‑1 (MQ=255)

                                                                                                   gTAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGAc                            <  1:439653/140‑1 (MQ=255)

                                                                                                    tAAACGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGAcc                           >  2:473752/1‑140 (MQ=255)

                                                                                                     aaaCGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCg                          <  1:473752/140‑1 (MQ=255)

                                                                                                       aCGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGtt                        <  1:256230/140‑1 (MQ=255)

                                                                                                        cGAAAGTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGttt                       <  1:1026042/140‑1 (MQ=255)

                                                                                                             gTCGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTtctc                  <  2:37062/140‑1 (MQ=255)

                                                                                                               cGGTACGCGAACGCCGCCTCCCCAGGTCGAGCCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCa                <  2:1301654/140‑1 (MQ=255)

                                                                                                               cGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCa                <  2:924640/140‑1 (MQ=255)

                                                                                                               cGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCa                <  2:11483/140‑1 (MQ=255)

                                                                                                               cGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCa                <  1:946724/140‑1 (MQ=255)

                                                                                                               cGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCa                <  1:778773/140‑1 (MQ=255)

                                                                                                               cGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCa                <  1:313359/140‑1 (MQ=255)

                                                                                                               cGGTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCa                <  1:171734/140‑1 (MQ=255)

                                                                                                                ggTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTtctc                  <  2:620879/137‑1 (MQ=255)

                                                                                                                ggTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTtctc                  >  1:620879/1‑137 (MQ=255)

                                                                                                                ggTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACAGTTTTTCTCCAg               >  2:641483/1‑140 (MQ=255)

                                                                                                                 gTACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCAgt              <  2:800161/140‑1 (MQ=255)

                                                                                                                  tACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCAgtg             <  1:1158432/140‑1 (MQ=255)

                                                                                                                  tACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCAgtg             <  2:292523/140‑1 (MQ=255)

                                                                                                                  tACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCAgtg             >  1:1166178/1‑140 (MQ=255)

                                                                                                                  tACGCGAACGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCAgtg             <  1:1241170/140‑1 (MQ=255)

                                                                                                                        aaCGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCAGTGTTTtat       <  2:1166178/140‑1 (MQ=255)

                                                                                                                         aCGCCGCCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCAGTGTTTtata      <  1:479808/140‑1 (MQ=255)

                                                                                                                          cgccgcCTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCAGTGTTTTATAc     >  1:1412720/1‑140 (MQ=255)

                                                                                                                             cgccTCCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCCTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCAGTGTTTTATACAGa  <  2:1382058/140‑1 (MQ=255)

TTTCGCTCCAGGATGCCCTGCCAGATCCAGCGCGGTAGGGAAGAGATCTGCCAGATCGACAATACCGTCAGATTTACGCGGTTGGATCATCCCTTTCCAGTAAACGAAAGTCGGTACGCGAACGCCGCCTTCCCAGGTCGAACCTTTCGCACCACGGAACGGGGTGCGTCCGTGCGGCGGTACTTCGGCTTCCGGTCCGTTATCGGAGGTAAAGACGATCAGCGTGTTATCAAGCTGACCGTTTTTCTCCAGTGTTTTATACAGA  >  NC_000913/3984739‑3985003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A