Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	151	87.4	2971636	99.2%	2947862	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,177,879	C→T	T46I (ACC→ATC)	nusG →	transcription termination factor NusG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,177,879	0	C	T	100.0%	278.8 / NA	78	T46I (ACC→ATC)	nusG	transcription termination factor NusG
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (40/38); total (40/38)

TGTCTGAAGCTCCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAACCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGC  >  NC_000913/4177744‑4178015

tGTCTGAAGCTCCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCgaa                                                                                                                                      <  2:1253984/140‑1 (MQ=255)

tGTCTGAAGCTCCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCgaa                                                                                                                                      <  1:327680/140‑1 (MQ=255)

  tCTGAAGCTCCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCgaaga                                                                                                                                    >  1:463719/1‑140 (MQ=255)

    tGAAGCTCCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGaagaag                                                                                                                                  <  1:1040622/140‑1 (MQ=255)

    tGAAGCTCCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGaagaag                                                                                                                                  <  2:367567/140‑1 (MQ=255)

    tGAAGCTCCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGaagaag                                                                                                                                  <  1:885431/140‑1 (MQ=255)

               aaaaGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATc                                                                                                                       <  2:140213/140‑1 (MQ=255)

                      cTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCg                                                                                                                >  1:1127303/1‑140 (MQ=255)

                      cTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCg                                                                                                                >  1:228029/1‑140 (MQ=255)

                      cTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCg                                                                                                                >  2:1350635/1‑140 (MQ=255)

                      cTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCg                                                                                                                >  2:1345168/1‑140 (MQ=255)

                      cTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCg                                                                                                                >  1:140679/1‑140 (MQ=255)

                           aCGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAg                                                                                                           <  2:1127303/140‑1 (MQ=255)

                                 ttCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGc                                                                                                     >  1:1046718/1‑140 (MQ=255)

                                 ttCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGc                                                                                                     >  1:791014/1‑140 (MQ=255)

                                 ttCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGc                                                                                                     >  1:1413726/1‑140 (MQ=255)

                                    aGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGGAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCaaa                                                                                                  >  1:691300/1‑140 (MQ=255)

                                         ttttCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGa                                                                                             >  2:85985/1‑140 (MQ=255)

                                           ttCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAAc                                                                                           >  2:1398036/1‑140 (MQ=255)

                                                gTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTaaa                                                                                      >  1:970667/1‑140 (MQ=255)

                                                       aGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTcc                                                                               <  2:1046718/140‑1 (MQ=255)

                                                       aGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTcc                                                                               <  1:85985/140‑1 (MQ=255)

                                                           cgcgTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTgg                                                                           >  2:429016/1‑140 (MQ=255)

                                                             cgTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCt                                                                         >  2:400823/1‑140 (MQ=255)

                                                             cgTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCt                                                                         >  2:1381129/1‑140 (MQ=255)

                                                             cgTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCt                                                                         >  1:923171/1‑140 (MQ=255)

                                                              gTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTa                                                                        <  2:844910/140‑1 (MQ=255)

                                                                  cAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTc                                                                    <  1:429016/140‑1 (MQ=255)

                                                                  cAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTc                                                                    <  1:1011508/140‑1 (MQ=255)

                                                                   aaCGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTcc                                                                   <  2:140679/140‑1 (MQ=255)

                                                                    aCGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAATGTAAATTCTTCCCTGGCTACGTCCt                                                                  <  2:1131764/140‑1 (MQ=255)

                                                                       tCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTcc                                                                               >  2:839615/1‑124 (MQ=255)

                                                                       tCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTcc                                                                               <  1:839615/124‑1 (MQ=255)

                                                                       tCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGt                                                               <  1:1086878/140‑1 (MQ=255)

                                                                       tCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGt                                                               <  1:383937/140‑1 (MQ=255)

                                                                        cGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGtt                                                              <  2:463719/140‑1 (MQ=255)

                                                                          cTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCa                                                            <  1:1398036/140‑1 (MQ=255)

                                                                            gCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGa                                                          <  2:1413726/140‑1 (MQ=255)

                                                                            gCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGa                                                          <  1:806192/140‑1 (MQ=255)

                                                                                     tatCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGtgatga                                                 >  1:592288/1‑140 (MQ=255)

                                                                                     tatCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACAAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTc                                                             <  1:1182982/128‑1 (MQ=255)

                                                                                     tatCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACAAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTc                                                             >  2:1182982/1‑128 (MQ=255)

                                                                                      atCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGaa                                                <  2:791014/140‑1 (MQ=255)

                                                                                       tCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAac                                               >  1:590698/1‑140 (MQ=255)

                                                                                           aTTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAacgacg                                           <  2:590698/140‑1 (MQ=255)

                                                                                           aTTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAacgacg                                           <  1:142008/140‑1 (MQ=255)

                                                                                               cacaACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAg                                       >  1:853569/1‑140 (MQ=255)

                                                                                               cacaACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAg                                       >  1:1415348/1‑140 (MQ=255)

                                                                                                acaacaTGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGc                                      >  1:860632/1‑140 (MQ=255)

                                                                                                acaacaTGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGc                                      >  2:1075313/1‑140 (MQ=255)

                                                                                                 caacaTGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCt                                     <  1:400823/140‑1 (MQ=255)

                                                                                                 caacaTGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCt                                     <  1:1075313/140‑1 (MQ=255)

                                                                                                    caTGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGc                                  >  1:809430/1‑140 (MQ=255)

                                                                                                    caTGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGc                                  <  1:1381129/140‑1 (MQ=255)

                                                                                                    caTGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGc                                  >  1:66089/1‑140 (MQ=255)

                                                                                                     aTGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCa                                 >  1:123661/1‑140 (MQ=255)

                                                                                                      tGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGtgatga                                                 <  2:1249382/123‑1 (MQ=255)

                                                                                                      tGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGtgatga                                                 >  1:1249382/1‑123 (MQ=255)

                                                                                                      tGGAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCAc                                <  2:1415348/140‑1 (MQ=255)

                                                                                                       ggAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTTCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCAcc                               >  2:972780/1‑140 (MQ=255)

                                                                                                       ggAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAacgacg                                           <  1:897192/128‑1 (MQ=255)

                                                                                                       ggAAGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAacgacg                                           >  2:897192/1‑128 (MQ=255)

                                                                                                         aaGATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTTAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTg                             >  1:107501/1‑140 (MQ=255)

                                                                                                           gATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGt                           <  2:809430/140‑1 (MQ=255)

                                                                                                           gATTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGt                           <  1:1350635/140‑1 (MQ=255)

                                                                                                            aTTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTg                          >  2:1060092/1‑140 (MQ=255)

                                                                                                            aTTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTg                          >  2:443887/1‑140 (MQ=255)

                                                                                                            aTTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTg                          >  1:818299/1‑140 (MQ=255)

                                                                                                            aTTTGTTTGGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTg                          >  1:1172807/1‑140 (MQ=255)

                                                                                                             tttgtttgGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTgc                         <  2:228029/140‑1 (MQ=255)

                                                                                                             tttgtttgGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTgc                         <  2:691300/140‑1 (MQ=255)

                                                                                                              ttgtttgGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTgcg                        >  2:178274/1‑140 (MQ=255)

                                                                                                              ttgtttgGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTgcg                        >  1:180862/1‑140 (MQ=255)

                                                                                                                   tgGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCg                   >  1:989428/1‑140 (MQ=255)

                                                                                                                   tgGTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCg                   <  1:443887/140‑1 (MQ=255)

                                                                                                                     gTGAAGTCATGGTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTa                 <  2:989428/140‑1 (MQ=255)

                                                                                                                               ggTACCAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGa       <  2:613916/140‑1 (MQ=255)

                                                                                                                                    cAATCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGc  >  1:985293/1‑140 (MQ=255)

TGTCTGAAGCTCCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCGTGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAACCGAAGAAGTGGTTGAAATCCGTGGCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGC  >  NC_000913/4177744‑4178015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A