Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F28 I1 R1 151 87.4 2971636 99.2% 2947862 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,178,045 G→A A101A (GCG→GCA)  nusG → transcription termination factor NusG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,178,0450GA97.7% 250.8 / NA 86A101A (GCG→GCA) nusGtranscription termination factor NusG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (34/50);  minor base C (1/1);  total (35/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCGCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGT  >  NC_000913/4177911‑4178180
                                                                                                                                      |                                                                                                                                       
cGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAAt                                                                                                                                    <  1:684837/140‑1 (MQ=255)
 gTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATc                                                                                                                                   <  1:926696/140‑1 (MQ=255)
     cAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCg                                                                                                                               >  1:943177/1‑140 (MQ=255)
     cAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCg                                                                                                                               <  1:644379/140‑1 (MQ=255)
      aaaaGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGa                                                                                                                              >  1:452563/1‑140 (MQ=255)
          gCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACAAAACAGCGATaaa                                                                                                                          >  2:1192446/1‑140 (MQ=255)
           cGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATaaaa                                                                                                                         >  2:313795/1‑139 (MQ=255)
           cGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAaag                                                                                                                         >  2:1129106/1‑140 (MQ=255)
             aaCGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAaagaa                                                                                                                       >  1:84282/1‑140 (MQ=255)
               cGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGt                                                                                                                     <  2:123661/140‑1 (MQ=255)
                    aTTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATg                                                                                                                <  2:1140438/140‑1 (MQ=255)
                    aTTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATg                                                                                                                <  2:580952/140‑1 (MQ=255)
                    aTTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATg                                                                                                                <  1:1345168/140‑1 (MQ=255)
                    aTTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATg                                                                                                                <  2:452563/140‑1 (MQ=255)
                    aTTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATg                                                                                                                <  1:1370138/140‑1 (MQ=255)
                    aTTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATg                                                                                                                <  2:261174/140‑1 (MQ=255)
                    aTTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATg                                                                                                                <  1:479556/140‑1 (MQ=255)
                          cccTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTTCTTCCCCAATCAGCGGTAAAGAAGCCGATGCGATTa                                                                                                          >  1:789002/1‑140 (MQ=255)
                          cccTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGGttt                                                                                                          >  1:1388077/1‑139 (MQ=255)
                             tGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGa                                                                                                       <  1:313795/140‑1 (MQ=255)
                             tGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGa                                                                                                       <  1:1129106/140‑1 (MQ=255)
                               gCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATg                                                                                                                >  1:76793/1‑129 (MQ=255)
                               gCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATg                                                                                                                <  2:76793/129‑1 (MQ=255)
                               gCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAAACGATGCGGTTATGAAc                                                                                                     >  2:1050624/1‑140 (MQ=255)
                                    gTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATC‑GCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTg                                                                                               >  1:833565/1‑140 (MQ=255)
                                     tCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTg                                                                                               <  2:107501/140‑1 (MQ=255)
                                       cTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTgca                                                                                             >  1:174259/1‑140 (MQ=255)
                                       cTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTgca                                                                                             >  1:999198/1‑140 (MQ=255)
                                        tCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTgcag                                                                                            <  2:1244686/140‑1 (MQ=255)
                                        ccGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTgcag                                                                                            <  1:1050624/139‑1 (MQ=255)
                                         cGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTGCGTATGGCCTGCGCCACTCTGCGGGAAAGAAGTCGACGCGTTTATTAACCGCCTgtagc                                                                                           >  2:1276209/1‑140 (MQ=255)
                                         cGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTgcagc                                                                                           <  1:296430/140‑1 (MQ=255)
                                                aTGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAAACAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTgg                                                                                    >  1:53455/1‑140 (MQ=255)
                                                  ggTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTg                                                                                  >  1:191744/1‑140 (MQ=255)
                                                    tgatgaACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGAt                                                                                >  2:1086548/1‑140 (MQ=255)
                                                              cgcgAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCCCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTc                                                                      >  1:290060/1‑140 (MQ=255)
                                                              cgcgAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCCATCAGCGATAAAAAAGTCGATGCGATTATGAACCGCCCGCAGCAGGGTGGGGATAAACCGCGTc                                                                      >  2:1450126/1‑140 (MQ=255)
                                                              cgcgAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGCGATAAGCCGCGTc                                                                      >  2:595085/1‑140 (MQ=255)
                                                              cgcgAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGGTGGGGATAAACCGCGTc                                                                      >  2:704229/1‑140 (MQ=255)
                                                              cgcgAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCCGCAGCAGGGTGGGGATAAGCCGCGTc                                                                      >  2:279645/1‑140 (MQ=255)
                                                                cgAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTTCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCg                                                                    >  1:1014163/1‑140 (MQ=255)
                                                                cgAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCg                                                                    >  2:112786/1‑140 (MQ=255)
                                                                     tGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAAc                                                               <  2:999198/140‑1 (MQ=255)
                                                                     tGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAAc                                                               <  2:174259/140‑1 (MQ=255)
                                                                     tGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAAc                                                               <  1:1693/140‑1 (MQ=255)
                                                                      ggCCCCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATGGGCGGTACTTCCGATCGTCCTGCACAAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACg                                                              <  2:965172/140‑1 (MQ=255)
                                                                      ggCCCCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACg                                                              <  1:1450126/140‑1 (MQ=255)
                                                                      ggCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACg                                                              <  2:473196/140‑1 (MQ=255)
                                                                       gCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGGGATAAGCCGCGTCCGAAAACGc                                                             >  2:816551/1‑140 (MQ=255)
                                                                             ggTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCTATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTg                                                       >  1:895966/1‑140 (MQ=255)
                                                                             ggTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAAAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTg                                                       >  2:491770/1‑140 (MQ=255)
                                                                               tGCGCAGCGTCCCGCGTGTGATGGGCTTCATCGCCGGTACTTCCGACCGTCCTGCCCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGac                                                     <  2:970667/140‑2 (MQ=255)
                                                                                   cAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCTATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCgg                                                 <  2:895966/140‑1 (MQ=255)
                                                                                        tACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGaa                                            >  1:901114/1‑140 (MQ=255)
                                                                                         cccGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGaaa                                           <  1:491770/139‑1 (MQ=255)
                                                                                         aCCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGaaa                                           >  1:1182696/1‑140 (MQ=255)
                                                                                           cgcgTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATg                                         <  2:1182696/140‑1 (MQ=255)
                                                                                            gcgTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATgg                                        <  1:137006/140‑1 (MQ=255)
                                                                                             cgTGTGATGGGCTTCATCGGCGGTATTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGt                                       <  1:972780/140‑1 (MQ=255)
                                                                                             cgTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGt                                       <  2:191744/140‑1 (MQ=255)
                                                                                             cgTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGt                                       <  2:901114/140‑1 (MQ=255)
                                                                                             acagtgATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGt                                       <  1:556415/137‑1 (MQ=255)
                                                                                              gtgtGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTc                                      <  1:471849/140‑1 (MQ=255)
                                                                                              gtgtGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTc                                      <  2:53455/140‑1 (MQ=255)
                                                                                                gtgATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGGTAAAGAAGACGATGCGATTATGAACCGCCTGCAGCAGGGTGGGGATAAGCCGCGTCCGAAAACGCTGTTTTAACCGGGGGAAATGGGCCg                                    >  2:471849/1‑140 (MQ=255)
                                                                                                gtgATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGaa                                                     >  1:567548/1‑123 (MQ=255)
                                                                                                gtgATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGaa                                                     <  2:567548/123‑1 (MQ=255)
                                                                                                 tgATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCgt                                   <  1:112786/140‑1 (MQ=255)
                                                                                                  gATGGGCTTCATC‑GCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCgtgt                                 <  2:833565/140‑1 (MQ=255)
                                                                                                   aTGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTc                                      >  1:156462/1‑135 (MQ=255)
                                                                                                   aTGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTc                                      <  2:156462/135‑1 (MQ=255)
                                                                                                   aTGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCgtgt                                 <  1:1086548/140‑1 (MQ=255)
                                                                                                   aTGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCgtgt                                 <  2:1014163/140‑1 (MQ=255)
                                                                                                   aTGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCgtgt                                 >  2:533768/1‑140 (MQ=255)
                                                                                                   aTGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGCGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCgtgt                                 <  1:595085/140‑1 (MQ=255)
                                                                                                     gggCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTa                               >  1:1136795/1‑140 (MQ=255)
                                                                                                       gCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGt                                       <  1:521711/130‑1 (MQ=255)
                                                                                                       gCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTc                                      <  2:464074/131‑1 (MQ=255)
                                                                                                       gCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTc                                      >  1:464074/1‑131 (MQ=255)
                                                                                                       gCTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGGGAAATGGt                                       >  2:521711/1‑130 (MQ=255)
                                                                                                        cTTCATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAatg                            >  1:553595/1‑140 (MQ=255)
                                                                                                           cATCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCCGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGGGAAATGGGCCGGGTTAatgatg                         >  2:234328/1‑140 (MQ=255)
                                                                                                            aTCGGCGGTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAACCGCGTCCGAAAAAGCTGTTTGAACCGGGGGAAATGGGCCGTGTTAatgctgt                        >  2:556415/1‑139 (MQ=255)
                                                                                                              cggcgtTACTTCCGATCGTCCTGCACCAATCAGGGATAAAGAAGTCGATGCGATTATGCACGGCCTGCAGGAGGTTGGTGATAAGCCGAGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTc                      <  2:985293/140‑1 (MQ=255)
                                                                                                                   gTACTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTc                 >  2:869672/1‑140 (MQ=255)
                                                                                                                      cTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCt              <  2:943177/140‑1 (MQ=255)
                                                                                                                      cTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCt              <  2:84282/140‑1 (MQ=255)
                                                                                                                      cTTCCGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGGGAAATGGTCCGTGTTAATGATGGTCCGTTCGCt              >  2:554433/1‑140 (MQ=255)
                                                                                                                          cGATCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACt          >  2:1406126/1‑140 (MQ=255)
                                                                                                                            aTCGTCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTc        >  1:1479593/1‑140 (MQ=255)
                                                                                                                                tCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCaa      <  2:1021524/138‑1 (MQ=255)
                                                                                                                                tCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCaa      >  1:1021524/1‑138 (MQ=255)
                                                                                                                                tCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACg    <  2:789002/140‑1 (MQ=255)
                                                                                                                                tCCTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACg    <  2:1136795/140‑1 (MQ=255)
                                                                                                                                 ccTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACgg   <  1:882864/140‑1 (MQ=255)
                                                                                                                                 ccTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACgg   >  2:882864/1‑140 (MQ=255)
                                                                                                                                  cTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGgt  <  2:553595/140‑1 (MQ=255)
                                                                                                                                  cTGCACCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGgt  <  1:554433/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
CGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGGCACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCGCCAATCAGCGATAAAGAAGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGT  >  NC_000913/4177911‑4178180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A