Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	151	87.4	2971636	99.2%	2947862	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,198,636	A→G	E307E (GAA→GAG)	hemE →	uroporphyrinogen decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,198,636	0	A	G	100.0%	414.7 / NA	115	E307E (GAA→GAG)	hemE	uroporphyrinogen decarboxylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (60/55); total (60/55)

Only 100 of 117 total aligned reads displayed.

ACCGGTTGCGATGCGCTGGGCCTCGACTGGACAACGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAAGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGACTGTCTGAACAGTAT  >  NC_000913/4198499‑4198771

aCCGGTTGCGATGCGCTGGGCCTCGACTGGACAACGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGt                                                                                                                                       <  2:438652/140‑1 (MQ=255)

              gcTGGGCCTCGACTGGACAACGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTg                                                                                                                         >  2:543278/1‑140 (MQ=255)

              gcTGGGCCTCGACTGGACAACGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTg                                                                                                                         >  1:1206890/1‑140 (MQ=255)

               cTGGGCCTCGACTGGACAACGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGc                                                                                                                        <  2:12701/140‑1 (MQ=255)

                  ggCCTCGACTGGACAACGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAgg                                                                                                                     >  1:1015751/1‑140 (MQ=255)

                  ggCCTCGACTGGACAACGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAgg                                                                                                                     >  2:308933/1‑140 (MQ=255)

                     cTCGACTGGACAACGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGttt                                                                                                                  <  1:473181/140‑1 (MQ=255)

                       cGACTGGACAACGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCg                                                                                                                >  2:667860/1‑140 (MQ=255)

                          cTGGACAACGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTc                                                                                                             <  2:1205952/140‑1 (MQ=255)

                            ggACAACGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCAc                                                                                                           >  2:631635/1‑140 (MQ=255)

                            ggACAACGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCAc                                                                                                           >  1:912689/1‑140 (MQ=255)

                             gACAACGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGGATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACg                                                                                                          >  2:1380896/1‑140 (MQ=255)

                              aCAACGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACgg                                                                                                         >  1:1374422/1‑140 (MQ=255)

                                aaCGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCg                                                                                                       <  2:537124/140‑1 (MQ=255)

                                      cATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTc                                                                                                 <  2:1316172/140‑1 (MQ=255)

                                       aTCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCa                                                                                                <  1:1423339/140‑1 (MQ=255)

                                       aTCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCa                                                                                                >  2:35519/1‑140 (MQ=255)

                                        tCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCAt                                                                                               <  2:1374422/140‑1 (MQ=255)

                                        tCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCAt                                                                                               >  1:606427/1‑140 (MQ=255)

                                        tCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCAt                                                                                               <  1:35519/140‑1 (MQ=255)

                                           ccGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCAt                                                                                               >  1:1012295/1‑137 (MQ=255)

                                           ccGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCAt                                                                                               <  2:1012295/137‑1 (MQ=255)

                                            cGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCt                                                                                           <  2:17415/140‑1 (MQ=255)

                                             gATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCtt                                                                                          <  2:631778/140‑1 (MQ=255)

                                                   cgccgcCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCt                                                                                    >  1:370301/1‑140 (MQ=255)

                                                         cgTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCa                                                                              <  2:42032/140‑1 (MQ=255)

                                                         cgTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCa                                                                              <  1:543278/140‑1 (MQ=255)

                                                              gggCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCa                                                                         <  1:308933/140‑1 (MQ=255)

                                                               ggCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCAt                                                                        <  2:538573/140‑1 (MQ=255)

                                                                   aTAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATtcat                                                                    <  1:146545/140‑1 (MQ=255)

                                                                    tAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCt                                                                                    <  2:1457279/123‑1 (MQ=255)

                                                                    tAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCt                                                                                    >  1:1457279/1‑123 (MQ=255)

                                                                     aaaGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATtcatca                                                                  >  1:356019/1‑140 (MQ=255)

                                                                          cgcgTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGAtg                                                             >  1:653222/1‑140 (MQ=255)

                                                                           gcgTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGAtgt                                                            <  2:1049262/140‑1 (MQ=255)

                                                                                   gggTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAg                                                    >  2:885013/1‑140 (MQ=255)

                                                                                   gggTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAg                                                    <  1:991560/140‑1 (MQ=255)

                                                                                      tAATATGGATCCGTCGATGCTGTACGCGCCCCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAAc                                                 <  2:912689/140‑1 (MQ=255)

                                                                                         tatGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATg                                              >  2:1143155/1‑140 (MQ=255)

                                                                                         tatGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATg                                              >  1:1171129/1‑140 (MQ=255)

                                                                                             gATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTgg                                          <  1:631635/140‑1 (MQ=255)

                                                                                              aTCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTgccg                                                        >  2:378049/1‑125 (MQ=255)

                                                                                              aTCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTgccg                                                        <  1:378049/125‑1 (MQ=255)

                                                                                              aTCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGc                                         <  2:142261/140‑1 (MQ=255)

                                                                                              aTCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGc                                         >  2:1440306/1‑140 (MQ=255)

                                                                                              aTCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGc                                         >  2:252034/1‑140 (MQ=255)

                                                                                              aTCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGc                                         >  2:770117/1‑140 (MQ=255)

                                                                                              aTCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGc                                         >  2:1373910/1‑140 (MQ=255)

                                                                                                ccGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCgt                                       >  2:1291142/1‑140 (MQ=255)

                                                                                                ccGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCgt                                       >  2:870552/1‑140 (MQ=255)

                                                                                                ccGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCgt                                       >  2:1454873/1‑140 (MQ=255)

                                                                                                ccGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCgt                                       >  2:292322/1‑140 (MQ=255)

                                                                                                ccGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCgt                                       >  1:1452029/1‑140 (MQ=255)

                                                                                                ccGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCgt                                       >  1:1334431/1‑140 (MQ=255)

                                                                                                  gTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATg                                              <  2:969990/131‑1 (MQ=255)

                                                                                                  gTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATg                                              >  1:969990/1‑131 (MQ=255)

                                                                                                  gTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCgtgt                                     >  1:437907/1‑140 (MQ=255)

                                                                                                  gTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCgtgt                                     >  1:1092114/1‑140 (MQ=255)

                                                                                                  gTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCgtgt                                     >  2:64187/1‑140 (MQ=255)

                                                                                                  gTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCgtgt                                     >  1:746767/1‑140 (MQ=255)

                                                                                                  gTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCgtg                                      >  1:1431229/1‑139 (MQ=255)

                                                                                                   tCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGtt                                    >  2:898299/1‑140 (MQ=255)

                                                                                                   tCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGtt                                    >  1:894511/1‑140 (MQ=255)

                                                                                                   tCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGtt                                    <  1:1268028/140‑1 (MQ=255)

                                                                                                   tCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGtt                                    >  1:801904/1‑140 (MQ=255)

                                                                                                   tCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGtt                                    >  2:1268028/1‑140 (MQ=255)

                                                                                                      aTGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGt                                 <  2:1092114/140‑1 (MQ=255)

                                                                                                      aTGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGt                                 <  1:870552/140‑1 (MQ=255)

                                                                                                       tGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTg                                <  1:884670/140‑1 (MQ=255)

                                                                                                            tACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGc                           <  1:898299/140‑1 (MQ=255)

                                                                                                            tACGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGc                           <  2:1364995/140‑1 (MQ=255)

                                                                                                             aCGCGCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCa                          >  2:145609/1‑140 (MQ=255)

                                                                                                              cgcgCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAg                         <  2:448180/140‑1 (MQ=255)

                                                                                                              cgcgCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAg                         >  1:133606/1‑140 (MQ=255)

                                                                                                              cgcgCCGCCTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAg                         >  1:430005/1‑140 (MQ=255)

                                                                                                                 gccgccTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTg                       <  1:232926/139‑1 (MQ=255)

                                                                                                                 gccgccTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGc                      >  2:880327/1‑140 (MQ=255)

                                                                                                                 gccgccTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGc                      >  2:30248/1‑140 (MQ=255)

                                                                                                                 gccgccTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGc                      >  1:1366871/1‑140 (MQ=255)

                                                                                                                 gccgccTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGc                      >  2:248211/1‑140 (MQ=255)

                                                                                                                   cgccTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCAt                    >  2:1373260/1‑140 (MQ=255)

                                                                                                                   cgccTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCAt                    >  2:167582/1‑140 (MQ=255)

                                                                                                                     ccTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCg                  <  1:292322/140‑1 (MQ=255)

                                                                                                                     ccTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCg                  <  2:894511/140‑1 (MQ=255)

                                                                                                                     ccTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCg                  <  1:880327/140‑1 (MQ=255)

                                                                                                                      cTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGa                 >  2:1076015/1‑140 (MQ=255)

                                                                                                                      cTGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGa                 >  2:1088763/1‑140 (MQ=255)

                                                                                                                       tGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGAc                <  1:30248/140‑1 (MQ=255)

                                                                                                                       tGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGAc                <  2:746767/140‑1 (MQ=255)

                                                                                                                       tGCCCGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGAc                <  1:1291142/140‑1 (MQ=255)

                                                                                                                         cccGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGc                           >  1:1147636/1‑127 (MQ=255)

                                                                                                                         cccGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGACTg              >  1:389347/1‑140 (MQ=255)

                                                                                                                          ccGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGACTGt             <  1:563745/140‑1 (MQ=255)

                                                                                                                           cGCATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGACTGTc            <  1:667860/140‑1 (MQ=255)

                                                                                                                             cATTGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGACTGTCTg          <  2:801904/140‑1 (MQ=255)

                                                                                                                               ttGAAGAAGTGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGACTGTCTGaa        <  2:504773/140‑1 (MQ=255)

                                                                                                                               ttGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGACTGTCTGaa        <  1:252034/140‑1 (MQ=255)

                                                                                                                                tGAAGAAGAGGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGACTGTCTGAAc       <  2:519366/140‑1 (MQ=255)

                                                                                                                                     aagaggTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGACTGTCTGAACAGTAt  <  1:770117/140‑1 (MQ=255)

                                                                                                                                     aagaggTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGACTGTCTGAACAGTAt  <  2:1418147/140‑1 (MQ=255)

ACCGGTTGCGATGCGCTGGGCCTCGACTGGACAACGGACATCGCCGATGCGCGCCGCCGTGTGGGCAATAAAGTCGCGTTGCAGGGTAATATGGATCCGTCGATGCTGTACGCGCCGCCTGCCCGCATTGAAGAAGAAGTAGCGACTATACTTGCAGGTTTCGGTCACGGCGAAGGTCATGTCTTTAACCTTGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGACTGTCTGAACAGTAT  >  NC_000913/4198499‑4198771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A