Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,199,676	C→T	T58M (ACG→ATG)	yjaG →	conserved protein YjaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,199,676	0	C	T	100.0%	124.8 / NA	36	T58M (ACG→ATG)	yjaG	conserved protein YjaG
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/16); total (20/16)

CTGGAAAGCTGGCAGCATGTCACTTTCATGGCTTGCTTATGCGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAACGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGCCGACGATTTCGATCTGTACGGCGTTTATCCGGCAATCGATGCCTGCGTGGCGTTAAGTGAACT  >  NC_000913/4199540‑4199811

cTGGAAAGCTGGCAGCATGTCACTTTCATGGCTTGCTTATGCGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCt                                                                                                                                      <  2:691870/140‑1 (MQ=255)

    aaaGCTGGCAGCATGTCACTTTCATGGCTTGCTTATGCGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGAcc                                                                                                                                  <  2:72707/140‑1 (MQ=255)

        cTGGCAGCATGTCACTTTCATGGCTTGCTTATGCGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGa                                                                                                                                    >  2:743626/1‑134 (MQ=255)

        cTGGCAGCATGTCACTTTCATGGCTTGCTTATGCGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGa                                                                                                                                    <  1:743626/134‑1 (MQ=255)

          ggCAGCATGTCACTTTCATGGCTTGCTTATGCGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTaaa                                                                                                                            >  1:250784/1‑140 (MQ=255)

                     aCTTTCATGGCTTGCTTATGCGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCa                                                                                                                      >  2:491388/1‑135 (MQ=255)

                     aCTTTCATGGCTTGCTTATGCGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCa                                                                                                                      <  1:491388/135‑1 (MQ=255)

                                   cttATGCGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGcc                                                                                                   <  2:857273/140‑1 (MQ=255)

                                   cttATGCGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGcc                                                                                                   >  2:54644/1‑140 (MQ=255)

                                   cttATGCGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGcc                                                                                                   >  2:254647/1‑140 (MQ=255)

                                   cttATGCGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGcc                                                                                                   >  2:145564/1‑140 (MQ=255)

                                   cttATGCGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGcc                                                                                                   >  1:857273/1‑140 (MQ=255)

                                     tATGCGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCaa                                                                                                 >  1:518267/1‑140 (MQ=255)

                                             cGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGaa                                                                                         >  2:183545/1‑140 (MQ=255)

                                                        cAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTTATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGaagaag                                                                              >  2:499323/1‑140 (MQ=255)

                                                          aTTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCg                                                                            >  1:671009/1‑140 (MQ=255)

                                                            tACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGAt                                                                          <  2:473922/140‑1 (MQ=255)

                                                             aCGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGAtt                                                                         >  1:316005/1‑140 (MQ=255)

                                                              cGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTc                                                                        >  1:106066/1‑140 (MQ=255)

                                                                ccATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCACATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTga                                                                                   >  1:451007/1‑127 (MQ=255)

                                                                ccATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCACATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTga                                                                                   <  2:451007/127‑1 (MQ=255)

                                                                     ttCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGc                                                                 >  2:680813/1‑140 (MQ=255)

                                                                       cTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGCcg                                                               <  1:54644/140‑1 (MQ=255)

                                                                        tGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGCcga                                                              <  1:145564/140‑1 (MQ=255)

                                                                        tGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGCcga                                                              <  1:183545/140‑1 (MQ=255)

                                                                                        ttGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGCCGACGATTTCGATCTGTAc                                              <  2:518267/140‑1 (MQ=255)

                                                                                                             gTCGCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGCCGACGATTTCGATCTGTACGGCGTTTATCCGGCAATCGAt                         <  2:316005/140‑1 (MQ=255)

                                                                                                                gCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGCCGACGATTTCGATCTGTACGGCGTTTATCCgg                                 <  2:280260/129‑1 (MQ=255)

                                                                                                                gCATTCTCGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGCCGACGATTTCGATCTGTACGGCGTTTATCCgg                                 >  1:280260/1‑129 (MQ=255)

                                                                                                                    tctcGTTCTCATCTGGGAAATGCTGGCAGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTGCAGCCGACGATTTCGATCTGTACGGCGTTTATCCGGCAATCGATGCCTGCg                  <  2:106066/140‑1 (MQ=255)

                                                                                                                      tcGATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGCCGACGATTTCGATCTGTACGGCGTTTATCCGGCAATCGATGCCTGCGTg                >  1:710707/1‑140 (MQ=255)

                                                                                                                        gATCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGCCGACGATTTCGATCTGTACGGCGTTTATCCGGCAATCGATGCCTGCGTGGc              >  2:383920/1‑140 (MQ=255)

                                                                                                                         aTCTCATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGCTGACGATTTCGATCTGTACGGCGTTTATCCGGCAATCGATGCCTGCGTGGCg             <  1:680813/140‑1 (MQ=255)

                                                                                                                             cATCTGGGAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGCCGACGATTTCGATCTGTACGGCGTTTATCCGGCAATCGATGCCTGCGTGGCGTTaa         <  2:710707/140‑1 (MQ=255)

                                                                                                                                   ggAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGCCGACGATTTCGATCTGTACGGCGTTTATCCGGCAATCGATGCCTGCGTGGc              >  2:3561/1‑129 (MQ=255)

                                                                                                                                   ggAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGCCGACGATTTCGATCTGTACGGCGTTTATCCGGCAATCGATGCCTGCGTGGc              <  1:3561/129‑1 (MQ=255)

                                                                                                                                    gAAATGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGAATCCTTCAGCCGACGATTTCGATCTGTACGGCGTTTATCCGGCAATCGATGCCTGCGTGGCGTTAAGTGAACt  >  2:803410/1‑140 (MQ=255)

CTGGAAAGCTGGCAGCATGTCACTTTCATGGCTTGCTTATGCGAACGCATGTACCCCAATTACGCCATGTTCTGCCAGCAAACCGGTTTTGGTGATGGGCAAATTTACCGTCGCATTCTCGATCTCATCTGGGAAACGCTGACCGTTAAAGACGCAAAAGTAAATTTCGACAGCCAACTGGAGAAATTTGAAGAAGCGATTCCTTCAGCCGACGATTTCGATCTGTACGGCGTTTATCCGGCAATCGATGCCTGCGTGGCGTTAAGTGAACT  >  NC_000913/4199540‑4199811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A