Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,200,155	(C)_6→5	intergenic (+61/‑126)	yjaG → / → hupA	conserved protein YjaG/DNA‑binding protein HU‑alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,200,150	0	C	.	93.1%	505.4 / 29.8	131	intergenic (+56/‑131)	yjaG/hupA	conserved protein YjaG/DNA‑binding protein HU‑alpha
Reads supporting (aligned to +/- strand): ref base C (5/4); new base . (63/59); total (68/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 150 total aligned reads displayed.

CGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTAT  >  NC_000913/4200011‑4200282

cgcgATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGc                                                                                                                                       >  2:1748373/1‑140 (MQ=255)

cgcgATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGc                                                                                                                                       <  2:1992498/140‑1 (MQ=255)

cgcgATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGc                                                                                                                                       >  2:1197730/1‑140 (MQ=255)

 gcgATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGcc                                                                                                                                      >  2:65735/1‑140 (MQ=255)

 gcgATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGcc                                                                                                                                      <  2:1572220/140‑1 (MQ=255)

     tatCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGcccccg                                                                                                                                  <  1:2290487/140‑2 (MQ=255)

      atCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGcccccgt                                                                                                                                 <  2:211284/140‑3 (MQ=255)

      atCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGcccccgt                                                                                                                                 <  2:951076/140‑3 (MQ=255)

      atCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGCTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGcccccgt                                                                                                                                 <  2:2236454/140‑3 (MQ=255)

       tCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGcccccgtc                                                                                                                                <  2:1764974/140‑4 (MQ=255)

       tCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGcccccgtc                                                                                                                                <  1:614974/140‑4 (MQ=255)

       tCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGcccccgtc                                                                                                                                <  1:1286331/140‑4 (MQ=255)

        cGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGcccccgtct                                                                                                                               >  2:2124776/1‑136 (MQ=255)

           gCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGt                                                                                                                           <  1:1827018/140‑1 (MQ=255)

            cTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTc                                                                                                                          <  1:1184269/140‑1 (MQ=255)

               aTAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGcccccgtc                                                                                                                                <  1:1908742/132‑4 (MQ=255)

               aTAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGcccccgt                                                                                                                                 >  2:1253816/1‑129 (MQ=255)

               aTAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTgg                                                                                                                            <  1:269207/135‑1 (MQ=255)

               aTAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTAc                                                                                                                       >  2:1192318/1‑140 (MQ=255)

               aTAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTAc                                                                                                                       >  2:1719939/1‑140 (MQ=255)

               aTAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTAc                                                                                                                       >  2:667792/1‑140 (MQ=255)

                    aGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGAATTAACGCCTGATTTGTCGTACCTGGAGTCTT‑CCTTTCG‑CCCCCGTCTGGTCTACATTTgg                                                                                                                 >  2:1867350/1‑140 (MQ=255)

                         ttAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTgggggg                                                                                                             >  2:1954241/1‑140 (MQ=255)

                         ttAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTgggggg                                                                                                             >  1:850189/1‑140 (MQ=255)

                         ttAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTgggggg                                                                                                             >  1:264960/1‑140 (MQ=255)

                                aGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGCCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑aaaaa                                                                                                     >  1:2286564/1‑140 (MQ=255)

                                 gACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑aaaaaa                                                                                                    >  1:1115345/1‑140 (MQ=255)

                                 gACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑aaaaaa                                                                                                    >  2:1251883/1‑140 (MQ=255)

                                  aCCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑aaaaaaa                                                                                                   >  1:1328174/1‑140 (MQ=255)

                                       cGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTgg                                                                                               >  1:1507108/1‑140 (MQ=255)

                                       cGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGc                                                                                              >  1:1612166/1‑140 (MQ=255)

                                          gAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTAt                                                                                           <  2:1672364/140‑1 (MQ=255)

                                          gAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGCCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTAt                                                                                           <  2:2286564/140‑1 (MQ=255)

                                          gAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTAACTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTCCATTTGGGGGGCG‑AAAAAAAGTGGCTAt                                                                                           <  2:814960/140‑1 (MQ=255)

                                           aGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTAt                                                                                           <  2:1507108/140‑1 (MQ=255)

                                             gCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCgg                                                                                        >  1:935140/1‑140 (MQ=255)

                                             gCGGGTGAGACCAACATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCgg                                                                                        <  2:2085006/140‑1 (MQ=255)

                                              cGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCgg                                                                                        >  1:1263156/1‑140 (MQ=255)

                                              cGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGt                                                                                       <  2:1904439/140‑1 (MQ=255)

                                              cGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGt                                                                                       >  1:321282/1‑140 (MQ=255)

                                                 gTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTT‑GGGGGCG‑AAAAAAAGTGGCTATCGGTGCgt                                                                                   >  2:670226/1‑140 (MQ=255)

                                                  tGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCgt                                                                                   <  2:1115345/140‑1 (MQ=255)

                                                  tGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCgt                                                                                   <  2:1587463/140‑1 (MQ=255)

                                                   gagaGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCgt                                                                                   <  2:895247/140‑1 (MQ=255)

                                                   gagaGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTT‑GGGGGCG‑AAAAAAAGTGGCTATCGGTGCgtgt                                                                                 <  1:670226/140‑1 (MQ=255)

                                                     gagCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTa                                                                                <  2:1685173/140‑1 (MQ=255)

                                                       gCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATg                                                                              <  1:667792/140‑1 (MQ=255)

                                                       gCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATg                                                                              <  1:148869/140‑1 (MQ=255)

                                                          atatTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAg                                                                           <  2:264960/140‑1 (MQ=255)

                                                           tatTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAg                                                                           >  1:595552/1‑140 (MQ=255)

                                                           tatTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAgg                                                                          >  2:498571/1‑140 (MQ=255)

                                                                gTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGt                                                                     >  1:849440/1‑140 (MQ=255)

                                                                 tataATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTg                                                                    <  2:849440/140‑1 (MQ=255)

                                                                  ataATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTg                                                                    <  1:1398353/140‑1 (MQ=255)

                                                                  ataATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGc                                                                   >  1:1092642/1‑140 (MQ=255)

                                                                  ataATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGc                                                                   <  1:1120803/140‑1 (MQ=255)

                                                                     aTTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGga                                                                         <  2:1150626/131‑1 (MQ=255)

                                                                     aTTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGga                                                                         >  1:1150626/1‑131 (MQ=255)

                                                                      tttttCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTAtt                                                               <  1:2124776/140‑1 (MQ=255)

                                                                              cAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCAtt                                                       >  1:1639645/1‑140 (MQ=255)

                                                                                    gACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTc                                                 <  1:812096/140‑1 (MQ=255)

                                                                                        aGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCAc                                             >  1:1675190/1‑140 (MQ=255)

                                                                                        aGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑‑CCCCGTCTGATCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACt                                            <  2:1672836/140‑1 (MQ=255)

                                                                                         gAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACt                                            >  2:1517196/1‑140 (MQ=255)

                                                                                          aaaaCGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTc                                           >  2:316814/1‑140 (MQ=255)

                                                                                               gTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CTCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGc                                      >  1:1749213/1‑140 (MQ=255)

                                                                                               gTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGc                                      >  2:2192981/1‑140 (MQ=255)

                                                                                               gTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGc                                      >  2:955266/1‑140 (MQ=255)

                                                                                                 gATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGc                                               >  2:1944840/1‑129 (MQ=255)

                                                                                                 gATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGc                                               <  1:1944840/129‑1 (MQ=255)

                                                                                                   tttAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAg                                  >  1:2035744/1‑140 (MQ=255)

                                                                                                    ttAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGc                                 <  2:1696995/140‑1 (MQ=255)

                                                                                                      aaCGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCaa                               <  2:328447/140‑1 (MQ=255)

                                                                                                      aaCGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCaa                               <  1:1251883/140‑1 (MQ=255)

                                                                                                      aaCGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCaa                               <  2:2009121/140‑1 (MQ=255)

                                                                                                           cTGATTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGAt                          <  1:1559693/140‑1 (MQ=255)

                                                                                                             gATTTGTCGTACCTGGAGTCTT‑CCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATaaa                       <  1:1867350/140‑1 (MQ=255)

                                                                                                              aTTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATaaa                       >  2:1820491/1‑140 (MQ=255)

                                                                                                              aTTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATaaa                       >  1:1736145/1‑140 (MQ=255)

                                                                                                              aTTTGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATaaa                       >  2:2075008/1‑140 (MQ=255)

                                                                                                                ttGTCGTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAaca                     <  1:2075008/140‑1 (MQ=255)

                                                                                                                     gTACCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTg                <  2:1736145/140‑1 (MQ=255)

                                                                                                                       aCCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAaca                     <  2:2213436/133‑1 (MQ=255)

                                                                                                                       aCCTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAaca                     >  1:2213436/1‑133 (MQ=255)

                                                                                                                        ccTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTa              >  2:1133351/1‑140 (MQ=255)

                                                                                                                         cTGGAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAg            <  1:498571/140‑1 (MQ=255)

                                                                                                                            gAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGAt         <  1:728656/140‑1 (MQ=255)

                                                                                                                            gAGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGAt         <  1:1954241/140‑1 (MQ=255)

                                                                                                                             aGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTa              >  2:905178/1‑134 (MQ=255)

                                                                                                                             aGTCTTCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTa              <  1:905178/134‑1 (MQ=255)

                                                                                                                                 ttCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACAtt                 >  1:1829200/1‑127 (MQ=255)

                                                                                                                                 ttCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACAtt                 <  2:1829200/127‑1 (MQ=255)

                                                                                                                                 ttCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGt               <  2:1118276/129‑1 (MQ=255)

                                                                                                                                 ttCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACtt    >  2:420272/1‑140 (MQ=255)

                                                                                                                                 ttCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACtt    >  1:2112481/1‑140 (MQ=255)

                                                                                                                                 ttCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACtt    >  1:2217085/1‑140 (MQ=255)

                                                                                                                                 ttCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACtt    >  1:500509/1‑140 (MQ=255)

                                                                                                                                  tCCCTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTa   >  2:295850/1‑140 (MQ=255)

                                                                                                                                   cccTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAATCACATTGTAAGGATAACTTAt  >  2:2194497/1‑140 (MQ=255)

                                                                                                                                   cccTTTCG‑CCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTAt  <  1:460005/140‑1 (MQ=255)

CGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCG‑AAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTAT  >  NC_000913/4200011‑4200282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A