Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	151	87.4	2971636	99.2%	2947862	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,224,381	A→G	D185G (GAC→GGC)	metH →	cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,224,381	0	A	G	100.0%	225.3 / NA	64	D185G (GAC→GGC)	metH	cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (38/26); total (38/26)

GGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCA  >  NC_000913/4224244‑4224517

ggCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGgca                                                                                                                                        <  1:740929/140‑1 (MQ=255)

  cGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCAcc                                                                                                                                      >  1:1181232/1‑140 (MQ=255)

  cGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCAcc                                                                                                                                      >  1:954031/1‑140 (MQ=255)

      tATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGATTTCGGCACCCTTa                                                                                                                                  <  1:363858/140‑1 (MQ=255)

              cGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCaaa                                                                                                                          >  2:238732/1‑140 (MQ=255)

              cGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCaaa                                                                                                                          >  2:634340/1‑140 (MQ=255)

               ggACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAg                                                                                                                         <  2:1181232/140‑1 (MQ=255)

                                  tttCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGc                                                                                                            <  1:840623/134‑1 (MQ=255)

                                  tttCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGc                                                                                                            >  2:840623/1‑134 (MQ=255)

                                     cGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCgg                                                                                                          <  2:1484307/133‑1 (MQ=255)

                                     cGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCgg                                                                                                          >  1:1484307/1‑133 (MQ=255)

                                     cGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAAc                                                                                                   >  1:168472/1‑140 (MQ=255)

                                     cGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAAc                                                                                                   >  1:87712/1‑140 (MQ=255)

                                          tatCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGt                                                                                              >  2:753865/1‑140 (MQ=255)

                                               cTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGaa                                                                                         >  1:1052328/1‑140 (MQ=255)

                                                          cTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGCTTGAAGCGCTGGGCGt                                                                              >  1:1262594/1‑140 (MQ=255)

                                                                gcggcTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCt                                                                        <  2:1262594/140‑1 (MQ=255)

                                                                 cggcTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTg                                                                       <  2:460610/140‑1 (MQ=255)

                                                                  ggcTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGc                                                                      <  2:1052328/140‑1 (MQ=255)

                                                                  ggcTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGc                                                                      <  2:1031490/140‑1 (MQ=255)

                                                                   gcTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGcc                                                                     >  2:133900/1‑140 (MQ=255)

                                                                    cTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCg                                                                    >  2:171864/1‑140 (MQ=255)

                                                                     ttATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGa                                                                   <  1:171864/140‑1 (MQ=255)

                                                                              gTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCGGCCGATTATGAtct                                                          >  2:916798/1‑140 (MQ=255)

                                                                               tCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGAtctc                                                         >  2:1379986/1‑140 (MQ=255)

                                                                                  accaAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGAtct                                                          <  2:646411/136‑1 (MQ=255)

                                                                                  accaAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGAtct                                                          >  1:646411/1‑136 (MQ=255)

                                                                                    caAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCa                                                    <  1:133900/140‑1 (MQ=255)

                                                                                              gtggAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAGAACGGAGTTGGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGa                                          >  2:1331865/1‑140 (MQ=255)

                                                                                                    ggTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTc                                    <  1:753865/140‑1 (MQ=255)

                                                                                                        gcgcGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCAc                                             >  2:53836/1‑127 (MQ=255)

                                                                                                        gcgcGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCAc                                             <  1:53836/127‑1 (MQ=255)

                                                                                                        gcgcGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACg                                        <  1:83640/132‑1 (MQ=255)

                                                                                                        gcgcGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACg                                        >  2:83640/1‑132 (MQ=255)

                                                                                                        gcgcGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCggg                                >  1:184311/1‑140 (MQ=255)

                                                                                                        gcgcGGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCggg                                >  1:143712/1‑140 (MQ=255)

                                                                                                         cgcgGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGgc                               >  1:854591/1‑140 (MQ=255)

                                                                                                           cgGATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGgcgc                             >  2:1179852/1‑140 (MQ=255)

                                                                                                           cgGATCTGATCCTGATTGAAACCGTTTTCGGCA‑CCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCa                            >  1:229976/1‑140 (MQ=255)

                                                                                                            ggATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCa                            <  1:1379986/140‑1 (MQ=255)

                                                                                                            ggATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCa                            <  1:193488/140‑1 (MQ=255)

                                                                                                            ggATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCa                            <  2:426116/140‑1 (MQ=255)

                                                                                                             gATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCAc                           <  2:954031/140‑1 (MQ=255)

                                                                                                             gATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCAc                           <  2:143712/140‑1 (MQ=255)

                                                                                                             gATCTGATCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCAc                           <  1:1331865/140‑1 (MQ=255)

                                                                                                                    tCCTGATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTcc                    <  1:209650/140‑1 (MQ=255)

                                                                                                                      cTGATTGAAACCGTTTTCGGCA‑CCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCggg                 <  2:229976/140‑1 (MQ=255)

                                                                                                                        gATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGc                >  1:584545/1‑140 (MQ=255)

                                                                                                                        gATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGc                >  1:1219898/1‑140 (MQ=255)

                                                                                                                        gATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGc                >  1:196404/1‑140 (MQ=255)

                                                                                                                        gATTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGAATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGc                >  1:920629/1‑140 (MQ=255)

                                                                                                                         aTTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCa               >  2:425041/1‑140 (MQ=255)

                                                                                                                         aTTGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCa               >  2:836586/1‑140 (MQ=255)

                                                                                                                          ttGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAg              >  2:1384591/1‑140 (MQ=255)

                                                                                                                          ttGAAACCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAg              >  1:190728/1‑140 (MQ=255)

                                                                                                                             aaaCCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCggg                 <  1:831884/134‑1 (MQ=255)

                                                                                                                             aaaCCGTTTTCGGCACCCTTAACGCCAAAGAGGCGGTATTTGCGGTGAAAACGGAGATTGAAGCGCTGGGCATTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCggg                 >  2:831884/1‑134 (MQ=255)

                                                                                                                              aaCCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAGacca          >  1:758970/1‑140 (MQ=255)

                                                                                                                              aaCCGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAGacca          >  1:533833/1‑140 (MQ=255)

                                                                                                                                 cGTTTTCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAGACCACCg       <  2:1219898/140‑1 (MQ=255)

                                                                                                                                   ttttCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGATGCCGATTATTATCTCCGGGACCATCCCCGCCGGCTCCGGGCGCACGCTCTCCGGGCAGACCACCGaa     >  2:171946/1‑140 (MQ=255)

                                                                                                                                    tttCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAGACCACCGAAg    >  2:341749/1‑140 (MQ=255)

                                                                                                                                     ttCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAGACCACCGAAGc   >  2:589935/1‑140 (MQ=255)

                                                                                                                                      tCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCa  <  1:634340/140‑1 (MQ=255)

                                                                                                                                      tCGGCACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCa  <  1:238732/140‑1 (MQ=255)

GGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGACGGGCTGGTGGCGGCTTATCGAGAGTCCACCAAAGCGCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCA  >  NC_000913/4224244‑4224517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A