Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,694,465	A→G	V536A (GTC→GCC)	uidA ←	beta‑glucuronidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,694,465	0	A	G	100.0%	185.1 / NA	51	V536A (GTC→GCC)	uidA	beta‑glucuronidase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/29); total (22/29)

GCCGCCGACTTCGGTTTGCGGTCGCGAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGACGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGTATCCACGCCGTATTCGGTGATGATAATCGGCTGATGCAGTTTCT  >  NC_000913/1694330‑1694591

gccgccGACTTCGGTTTGCGGTCGCGAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGcgcg                                                                                                                            <  2:773441/140‑1 (MQ=255)

gccgccGACTTCGGTTTGCGGTCGCGAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGcgcg                                                                                                                            <  2:410777/140‑1 (MQ=255)

gccgccGACTTCGGTTTGCGGTCGCGAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGcgcg                                                                                                                            <  2:1122040/140‑1 (MQ=255)

  cgccgACTTCGGTTTGCGGTCGCGAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGt                                                                                                                          <  2:1569729/140‑1 (MQ=255)

       aCTTCGGTTTGCGGTCGCGAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATAc                                                                                                                     <  1:461546/140‑1 (MQ=255)

        cTTCGGTTTGCGGTCGCGAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGcg                                                                                                                              >  2:1011202/1‑130 (MQ=255)

                 gCGGTCGCGAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGcc                                                                                                           <  2:1343781/140‑1 (MQ=255)

                    gTCGCGAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATg                                                                                                        <  2:132820/140‑1 (MQ=255)

                    gTCGCGAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATg                                                                                                        <  1:38603/140‑1 (MQ=255)

                    gTCGCGAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATg                                                                                                        <  1:849747/140‑1 (MQ=255)

                      cgcgAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGca                                                                                                      <  1:463977/140‑1 (MQ=255)

                        cgAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCAATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGcaca                                                                                                    >  2:807612/1‑140 (MQ=255)

                         gAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAATGCGCGGTGATACATATCCAGCCATGCacac                                                                                                   <  1:867037/140‑1 (MQ=255)

                         gAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCacac                                                                                                   <  1:257001/140‑1 (MQ=255)

                         gAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCacac                                                                                                   <  1:587274/140‑1 (MQ=255)

                              aaGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATa                                                                                              <  1:1455020/140‑1 (MQ=255)

                                 aTCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATActc                                                                                           >  2:1140899/1‑140 (MQ=255)

                                   cccTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCtt                                                                                         <  1:56029/140‑1 (MQ=255)

                                     cTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCa                                                                                       >  2:755711/1‑140 (MQ=255)

                                     cTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCa                                                                                       <  1:614542/140‑1 (MQ=255)

                                           tGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTg                                                                                                 >  2:514138/1‑124 (MQ=255)

                                           tGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTg                                                                                                 <  1:514138/124‑1 (MQ=255)

                                               aCCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCa                                                                                       >  1:617910/1‑130 (MQ=255)

                                               aCCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCa                                                                                       <  2:617910/130‑1 (MQ=255)

                                                ccGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTc                                                                            <  2:886482/140‑1 (MQ=255)

                                                ccGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTc                                                                            <  1:1625010/140‑1 (MQ=255)

                                                   ccAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGgt                                                                         <  1:8037/140‑1 (MQ=255)

                                                       cgcgCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTAc                                                                     >  2:910149/1‑140 (MQ=255)

                                                       cgcgCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTAc                                                                     >  2:580848/1‑140 (MQ=255)

                                                        gcgcAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACa                                                                    <  2:825695/140‑1 (MQ=255)

                                                                        gAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGc                                                    >  1:1671020/1‑140 (MQ=255)

                                                                        gAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGc                                                    >  1:1617023/1‑140 (MQ=255)

                                                                          ggTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTa                                                  >  2:1198631/1‑140 (MQ=255)

                                                                            tCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAAc                                                >  1:1361294/1‑140 (MQ=255)

                                                                              gCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGt                                              >  2:16633/1‑140 (MQ=255)

                                                                                     cGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGTATCCACg                                       <  1:809737/140‑1 (MQ=255)

                                                                                     cGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGTATCCACg                                       <  1:998671/140‑1 (MQ=255)

                                                                                     cGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGTATCCACg                                       <  2:1617023/140‑1 (MQ=255)

                                                                                      ggCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGcc                                                        <  2:743924/122‑1 (MQ=255)

                                                                                      ggCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGcc                                                        >  1:743924/1‑122 (MQ=255)

                                                                                         gAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGTATCCAc                                        <  1:824623/135‑1 (MQ=255)

                                                                                         gAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGTATCCAc                                        >  2:824623/1‑135 (MQ=255)

                                                                                                  tACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGCACATTGAGTGCAGCCCGGCTAACGTATCCACGCCGTATTCGGtga                          >  1:768344/1‑140 (MQ=255)

                                                                                                        ttCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTATATTGAGTGCAGCCCGGCTAACGTATCCACGCCGTATTCGGTGATGATaa                    >  1:385742/1‑140 (MQ=255)

                                                                                                        ttCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGTATCCACGCCGTATTCGGTGATGATaa                    >  2:1597869/1‑140 (MQ=255)

                                                                                                        ttCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGTATCCACGCCGTATTCGGTGATGATaa                    >  1:270585/1‑140 (MQ=255)

                                                                                                        ttCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGTATCCACGCCGTATTCGGTGATGATaa                    >  2:1260905/1‑140 (MQ=255)

                                                                                                        ttCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGTATCCACGCCGTATTCGGTGATGATaa                    >  1:1535061/1‑140 (MQ=255)

                                                                                                        ttCACCGACGACGGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGTATCCACGCCGTATTCGGTGATGATaa                    <  1:755711/140‑1 (MQ=255)

                                                                                                                  acgGCGCTGACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGTATCCACGCCGTATTCGGTGATGATAATCGGCTGATg          >  1:949959/1‑140 (MQ=255)

                                                                                                                          gACGCGATCAAAGGCGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGTATCCACGCCGTATTCGGTGATGATAATCGGCTGATGCAGTTtct  <  1:807612/140‑1 (MQ=255)

GCCGCCGACTTCGGTTTGCGGTCGCGAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGACGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGTATCCACGCCGTATTCGGTGATGATAATCGGCTGATGCAGTTTCT  >  NC_000913/1694330‑1694591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A