Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F28 I1 R1
|
183 |
98.6 |
3427438 |
96.9% |
3321187 |
138.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,057,676 |
(C)7→6 |
intergenic (+102/+351) |
yeeN → / ← asnW |
putative transcriptional regulator YeeN/tRNA‑Asn |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,057,670 | 0 | C | . | 98.3%
| 249.0
/ ‑2.7
| 60 | intergenic (+96/+357) | yeeN/asnW | putative transcriptional regulator YeeN/tRNA‑Asn |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (32/27); total (32/28) |
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTC > NC_000913/2057532‑2057805
|
cgacgaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAcc > 2:961436/1‑140 (MQ=255)
gTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAccccccgtt < 1:961436/140‑4 (MQ=255)
aaaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCa < 2:626015/140‑1 (MQ=255)
aaaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCa < 2:607100/140‑1 (MQ=255)
aaaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCa < 1:370705/140‑1 (MQ=255)
aaaaaGTTTATCATAACCTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCa < 1:1483834/140‑1 (MQ=255)
aaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCag < 2:361797/140‑1 (MQ=255)
aaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCCTCATATTGATTATA‑CCCCCCGTTCCCag < 2:855421/140‑1 (MQ=255)
aaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCaga > 2:1657709/1‑140 (MQ=255)
gTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGaa > 1:1072534/1‑140 (MQ=255)
ttATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTcc > 1:439394/1‑131 (MQ=255)
ttATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGaaa > 2:647157/1‑139 (MQ=255)
ttATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGaaa < 1:647157/139‑1 (MQ=255)
ttATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAaat > 2:1046893/1‑140 (MQ=255)
ttATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATCGATTATA‑CCCCCCGTTcc < 2:439394/131‑1 (MQ=255)
ctctAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCa > 1:959155/1‑140 (MQ=255)
aaTTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttc < 1:802938/140‑1 (MQ=255)
cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCttt > 1:1047778/1‑140 (MQ=255)
cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCttt > 1:1685313/1‑140 (MQ=255)
ttttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCtttt > 1:1478645/1‑140 (MQ=255)
aGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGAt > 2:682611/1‑140 (MQ=255)
ttACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCaa > 2:78881/1‑140 (MQ=255)
cGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGt < 1:1056453/140‑1 (MQ=255)
gCAGATTTCTTTAATCTCATATAATTCTTACAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTgg < 2:1047778/140‑1 (MQ=255)
aGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATA‑TTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCaa > 2:1305952/1‑140 (MQ=255)
aTTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAAt > 1:598964/1‑140 (MQ=255)
ttAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCa < 1:1657709/140‑1 (MQ=255)
ttAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTGCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCa < 2:1175906/140‑1 (MQ=255)
tctcATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGt > 1:186547/1‑140 (MQ=255)
atatAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTcc > 1:294247/1‑140 (MQ=255)
tataATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGt > 2:572447/1‑135 (MQ=255)
tataATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGt < 1:572447/135‑1 (MQ=255)
tataATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTccc > 1:1231049/1‑140 (MQ=255)
aaTTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGAt < 2:186547/140‑1 (MQ=255)
cTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAg > 1:225178/1‑140 (MQ=255)
tataAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGttt < 2:1526829/128‑1 (MQ=255)
tataAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGttt > 1:1526829/1‑128 (MQ=255)
tataAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGAc > 1:1205463/1‑140 (MQ=255)
ataAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGAcg > 2:261708/1‑140 (MQ=255)
tataatatTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCg < 2:959155/140‑1 (MQ=255)
tataatatTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCg < 2:1072534/140‑1 (MQ=255)
taatatTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGAc < 2:1156496/140‑1 (MQ=255)
tatTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACa < 1:943499/138‑1 (MQ=255)
tatTCAACTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACa > 2:943499/1‑138 (MQ=255)
aaCTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCa > 1:492939/1‑140 (MQ=255)
aaCTCGTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCa > 1:592370/1‑140 (MQ=255)
gTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTACAATTCCAGTTCTTCTTTTTCTGCTTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCg > 2:787797/1‑125 (MQ=255)
gTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCg < 1:787797/125‑1 (MQ=255)
gTCATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAgg > 1:925143/1‑140 (MQ=255)
cATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCg > 1:382695/1‑123 (MQ=255)
cATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCg < 2:382695/123‑1 (MQ=255)
cATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCa > 1:1687101/1‑140 (MQ=255)
cATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCa > 1:502984/1‑140 (MQ=255)
cATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCa > 1:938772/1‑140 (MQ=255)
cATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATACCGCATAAg < 1:129539/137‑1 (MQ=255)
cATATTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATACCGCATAAg > 2:129539/1‑137 (MQ=255)
atTGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCAttt < 2:592370/140‑1 (MQ=255)
ttGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCAtttt < 2:492939/140‑1 (MQ=255)
cagATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCAttt < 2:278392/138‑1 (MQ=255)
tGATTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCAttttt < 1:1046893/140‑1 (MQ=255)
aTTATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTtct < 2:925143/140‑1 (MQ=255)
ttATA‑CCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTtctc > 1:1018193/1‑140 (MQ=255)
|
CGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTC > NC_000913/2057532‑2057805
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
