Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,185,875	(G)_7→8	intergenic (‑79/‑42)	rcnR ← / → rcnA	DNA‑binding transcriptional repressor RcnR/Ni(2(+))/Co(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,185,868	1	.	G	100.0%	171.5 / NA	49	intergenic (‑72/‑49)	rcnR/rcnA	DNA‑binding transcriptional repressor RcnR/Ni(2(+))/Co(2(+)) exporter
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (31/18); total (31/18)

CCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCT  >  NC_000913/2185733‑2186002

ccTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑gggg                                                                                                                                    >  2:1477570/1‑140 (MQ=255)

  tGGCCATGAATCTGACTGGCAGGCGCTTTAAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACAACTGGTTAACAAGAATATGAAAAATCATAGCACTATTAAGCTACTGGGGGGTAGTATCAGGTACT‑‑gggggg                                                                                                                                  <  2:1064379/140‑1 (MQ=255)

      ccTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑ggggggggag                                                                                                                              <  1:1302771/140‑4 (MQ=255)

          aaTCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGa                                                                                                                           >  1:670078/1‑140 (MQ=255)

               tACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑gggggg                                                                                                                                  <  2:44845/127‑1 (MQ=255)

               tACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑gggggg                                                                                                                                  >  1:44845/1‑127 (MQ=255)

                    gCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGc                                                                                                                 <  2:908232/140‑1 (MQ=255)

                      aCGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGGAGTAGAATCAGATTGcc                                                                                                                >  1:879813/1‑140 (MQ=255)

                      aCGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCg                                                                                                               >  2:1611713/1‑140 (MQ=255)

                                aGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACt                                                                                                     <  1:286450/140‑1 (MQ=255)

                                        tttATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAatta                                                                                             >  1:1217239/1‑140 (MQ=255)

                                                ggATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATg                                                                                     >  2:1306303/1‑140 (MQ=255)

                                                  aTTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTGCTGGGGGGTAGTAGCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGAc                                                                                   <  1:608448/140‑1 (MQ=255)

                                                  aTTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATg                                                                                     <  2:655904/138‑1 (MQ=255)

                                                  aTTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATg                                                                                     >  1:655904/1‑138 (MQ=255)

                                                     gTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCg                                                                                 >  2:1085586/1‑140 (MQ=255)

                                                     gTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGa                                                                                <  2:1336087/140‑1 (MQ=255)

                                                              aTGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAAc                                                                       >  2:1020565/1‑140 (MQ=255)

                                                                ggCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActc                                                                     >  2:1275477/1‑140 (MQ=255)

                                                                ggCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActc                                                                     >  1:498424/1‑140 (MQ=255)

                                                                ggCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActc                                                                     >  1:532029/1‑140 (MQ=255)

                                                                ggCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActc                                                                     >  2:1300223/1‑140 (MQ=255)

                                                                         cggTTAATAAGAATATGAAAAATCATGGCACTATTATTCCACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCTGc                                                            <  2:670078/139‑1 (MQ=255)

                                                                             tAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAgg                                                        <  1:1306303/140‑1 (MQ=255)

                                                                                cAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCa                                                              <  2:470327/131‑1 (MQ=255)

                                                                                cAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCa                                                              >  1:470327/1‑131 (MQ=255)

                                                                                     atatGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGcc                                                 <  1:1085586/140‑1 (MQ=255)

                                                                                       atGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTgg                                              <  1:1477570/140‑1 (MQ=255)

                                                                                        tGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCTTGGt                                             <  1:1275477/140‑1 (MQ=255)

                                                                                        tGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGt                                             >  1:1441992/1‑140 (MQ=255)

                                                                                         gAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGtt                                            >  2:842674/1‑140 (MQ=255)

                                                                                             aaTCATAGGATGAATATTCGACTGGGGGGTAGTACAAGGTAGTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGttcttc                                        <  2:1185110/140‑1 (MQ=255)

                                                                                                    gCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCa                                 >  1:1185110/1‑140 (MQ=255)

                                                                                                      ccTATTTATCTACTGGGGGGTAGTATCAGGTAATG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAgc                               <  2:1646319/139‑1 (MQ=255)

                                                                                                               cTACTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTa                      >  1:697378/1‑140 (MQ=255)

                                                                                                                  cTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTa                      <  1:842708/137‑1 (MQ=255)

                                                                                                                  cTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTa                      >  2:842708/1‑137 (MQ=255)

                                                                                                                  cTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACtt                   >  2:716264/1‑140 (MQ=255)

                                                                                                                  cTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACtt                   >  1:360356/1‑140 (MQ=255)

                                                                                                                  cTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACtt                   >  1:932132/1‑140 (MQ=255)

                                                                                                                  cTGGGGGGTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACtt                   >  2:269631/1‑140 (MQ=255)

                                                                                                                    ggggggTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGTATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTgg                 >  1:1071087/1‑140 (MQ=255)

                                                                                                                    ggggggTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTgg                 >  1:1050282/1‑140 (MQ=255)

                                                                                                                    ggggggTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTgg                 >  1:691794/1‑140 (MQ=255)

                                                                                                                    ggggggTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTgg                 >  1:1587421/1‑140 (MQ=255)

                                                                                                                    ggggggTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTgg                 >  1:1398017/1‑140 (MQ=255)

                                                                                                                       gggTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTgc              <  2:697378/140‑1 (MQ=255)

                                                                                                                        ggTAGTATCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTgcg             >  2:258975/1‑140 (MQ=255)

                                                                                                                            gtaTCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTc         >  2:647628/1‑140 (MQ=255)

                                                                                                                             taTCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCa        >  1:899709/1‑140 (MQ=255)

                                                                                                                              aTCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCAt       <  1:716264/140‑1 (MQ=255)

                                                                                                                              aTCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCAt       <  1:1611713/140‑1 (MQ=255)

                                                                                                                              aTCAGGTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCAt       <  2:932132/140‑1 (MQ=255)

                                                                                                                                  ggTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGcc   <  2:360356/140‑1 (MQ=255)

                                                                                                                                  ggTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGcc   <  2:1217239/140‑1 (MQ=255)

                                                                                                                                  ggTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGcc   >  1:1553393/1‑140 (MQ=255)

                                                                                                                                   gTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCt  <  2:616140/140‑1 (MQ=255)

                                                                                                                                   gTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCt  <  2:1553393/140‑1 (MQ=255)

                                                                                                                                   gTACTG‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCt  >  1:616140/1‑140 (MQ=255)

CCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑GGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCATGGCCT  >  NC_000913/2185733‑2186002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A