Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 183 98.6 3427438 96.9% 3321187 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,432,759 T→C T62A (ACC→GCC)  folC ← bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,432,7590TC100.0% 243.3 / NA 66T62A (ACC→GCC) folCbifunctional folylpolyglutamate synthetase/dihydrofolate synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/29);  total (37/29)

GCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAG  >  NC_000913/2432623‑2432894
                                                                                                                                         |                                                                                                                                       
gCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCCGGTGGGGcc                                                                                                                                      >  2:1083476/1‑140 (MQ=255)
 cGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCt                                                                                                                                     <  1:724119/140‑1 (MQ=255)
    tgtgGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTg                                                                                                                                  <  1:239987/140‑1 (MQ=255)
     gtgGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGc                                                                                                                                 <  2:671784/140‑1 (MQ=255)
         gCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGGATAACGCACCAGATGAGGGGAACTGTAGACGCCCCCTGTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGGGGGGCCCTTTCCAtt                                                                                                                             >  2:1497908/1‑140 (MQ=255)
             aTTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTa                                                                                                                         >  1:1530017/1‑140 (MQ=255)
               tCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTAcc                                                                                                                       <  1:1494516/140‑1 (MQ=255)
               tCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTAcc                                                                                                                       <  1:637497/140‑1 (MQ=255)
                   gCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCa                                                                                                                   <  1:257318/140‑1 (MQ=255)
                      aTTCCTGGCCCTGCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACg                                                                                                                <  1:1229149/140‑1 (MQ=255)
                         ccTGGCCCTGCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTa                                                                                                             <  1:918575/140‑1 (MQ=255)
                                 tGCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACaaa                                                                                                     >  2:270851/1‑140 (MQ=255)
                                  gCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt                                                                                                    >  1:143971/1‑140 (MQ=255)
                                   cacaCGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATg                                                                                                   >  1:1346240/1‑140 (MQ=255)
                                             cgcTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGGAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGttt                                                                                         >  2:1530122/1‑140 (MQ=255)
                                                tCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAg                                                                                      >  2:1632952/1‑140 (MQ=255)
                                                            aCCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcg                                                                          >  1:1688507/1‑140 (MQ=255)
                                                            aCCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGAAAACACAAATGGCGCGGGTTTCAGGACGCCacagcg                                                                          >  2:1458384/1‑135 (MQ=255)
                                                              cAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGa                                                                                    <  1:130904/128‑1 (MQ=255)
                                                              cAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGa                                                                                    >  2:130904/1‑128 (MQ=255)
                                                              cAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGcc                                                                                >  1:1114968/1‑132 (MQ=255)
                                                              cAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGcc                                                                                <  2:1114968/132‑1 (MQ=255)
                                                              cAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcgcg                                                                        >  2:283065/1‑140 (MQ=255)
                                                              cAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcgcg                                                                        >  2:941952/1‑140 (MQ=255)
                                                              cAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcgcg                                                                        >  1:557845/1‑140 (MQ=255)
                                                              cAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcgcg                                                                        >  2:1510620/1‑140 (MQ=255)
                                                                   gAGGCGAGCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCcgcg                                                                   >  1:897370/1‑140 (MQ=255)
                                                                     ggCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc                                                                 <  2:1346240/140‑1 (MQ=255)
                                                                      gCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGCAC‑GGCAGGTGGCCCCTTTGCCATTCGTACCCGCAACGGTCAACACAAATGGCGCTGGCTTCAGGACGCCAAGACGCGCCGCGAcc                                                                >  2:1022231/1‑140 (MQ=255)
                                                                        gAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAg                                                              >  1:1465529/1‑140 (MQ=255)
                                                                         aaCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAgg                                                             <  1:1623622/140‑1 (MQ=255)
                                                                          aCTGTAGACGCCCACTTTGTACCCTGCCGCCATCTGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGc                                                            >  2:1517121/1‑140 (MQ=255)
                                                                             gTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCa                                                         <  2:504027/140‑1 (MQ=255)
                                                                                gACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCAcgc                                                      <  2:1465529/140‑1 (MQ=255)
                                                                                   gCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCt                                                   >  1:1017786/1‑140 (MQ=255)
                                                                                    cccACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTc                                                  <  2:6058/140‑1 (MQ=255)
                                                                                    cccACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTc                                                  >  2:200097/1‑140 (MQ=255)
                                                                                     ccACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCa                                                 >  1:655696/1‑140 (MQ=255)
                                                                                      cACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAgg                                                             <  2:1146122/127‑1 (MQ=255)
                                                                                      cACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAgg                                                             >  1:1146122/1‑127 (MQ=255)
                                                                                      cACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCt                                                   <  2:96363/137‑1 (MQ=255)
                                                                                      cACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCt                                                   >  1:96363/1‑137 (MQ=255)
                                                                                      cACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCaa                                                >  2:125959/1‑140 (MQ=255)
                                                                                      cACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGAAAACACAAAGGGCGCGGGTTTCAGGACCCCAAGACGCCCCGCGACCAGGCTCACGCGCTCaa                                                >  2:1301291/1‑140 (MQ=255)
                                                                                        cTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAgg                                              <  2:897370/140‑1 (MQ=255)
                                                                                        cTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAgg                                              >  1:1215691/1‑140 (MQ=255)
                                                                                        cTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAgg                                              >  2:22340/1‑140 (MQ=255)
                                                                                        cTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAgg                                              >  1:1409729/1‑140 (MQ=255)
                                                                                          ttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGcc                                            <  2:1017786/140‑1 (MQ=255)
                                                                                             tACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCgag                                         >  2:821331/1‑140 (MQ=255)
                                                                                               cccTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGAt                                       >  2:707586/1‑140 (MQ=255)
                                                                                                ccTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATc                                      <  1:139016/140‑1 (MQ=255)
                                                                                                  tGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGa                                    >  2:1243782/1‑140 (MQ=255)
                                                                                                  tGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGa                                    >  1:992103/1‑140 (MQ=255)
                                                                                                  tGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGa                                    <  1:1632952/140‑1 (MQ=255)
                                                                                                  tGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGa                                    <  1:536311/140‑1 (MQ=255)
                                                                                                  tGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGa                                    <  1:707586/140‑1 (MQ=255)
                                                                                                   gccgccATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGAt                                   >  2:71730/1‑140 (MQ=255)
                                                                                                                  ggcTCCAGCGTACGGGCGGGTGCGCCTTTTGCCTTTCTTCCCCG‑AACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAg                    <  1:1301291/138‑1 (MQ=255)
                                                                                                                   aCTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAgg                   >  1:1218266/1‑140 (MQ=255)
                                                                                                                   aCTCCAGCGTAC‑GGCAGGTGGCGCC‑TTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGt                  >  2:82396/1‑140 (MQ=255)
                                                                                                                    cTCCAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGt                  >  1:358366/1‑140 (MQ=255)
                                                                                                                       cAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGTCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGtttt               <  1:1510620/140‑1 (MQ=255)
                                                                                                                       cAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGtttt               <  2:1409729/140‑1 (MQ=255)
                                                                                                                       cAGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGtttt               <  2:1215691/140‑1 (MQ=255)
                                                                                                                        aGCGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTc              >  2:1632927/1‑140 (MQ=255)
                                                                                                                          cGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGtttt               >  1:764460/1‑137 (MQ=255)
                                                                                                                          cGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGtttt               <  2:764460/137‑1 (MQ=255)
                                                                                                                          cGTAC‑GGCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCa            <  2:655696/140‑1 (MQ=255)
                                                                                                                                ggCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGG‑TTTCCAGa          >  2:1662773/1‑136 (MQ=255)
                                                                                                                                ggCAGGTGGCGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGG‑TTTCCAGa          <  1:1662773/136‑1 (MQ=255)
                                                                                                                                    ggtggcGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGaaa   <  1:1632927/140‑1 (MQ=255)
                                                                                                                                     gtggcgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAg  >  2:1246080/1‑140 (MQ=255)
                                                                                                                                     gtggcgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAg  >  1:1638618/1‑140 (MQ=255)
                                                                                                                                     gtggcgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGACCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAg  >  2:808557/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTAC‑GGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGACCAGGCTCACGCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAG  >  NC_000913/2432623‑2432894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A