Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,436,415	T→C	T79A (ACC→GCC)	usg ←	putative semialdehyde dehydrogenase Usg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,436,415	0	T	C	100.0%	106.0 / NA	31	T79A (ACC→GCC)	usg	putative semialdehyde dehydrogenase Usg
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/15); total (16/15)

GTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATA  >  NC_000913/2436287‑2436547

gtAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGc                                                                                                                           <  2:192710/140‑1 (MQ=255)

gtAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGc                                                                                                                           >  1:192710/1‑140 (MQ=255)

          aGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACaaa                                                                                                                 >  2:344202/1‑140 (MQ=255)

               aaaCGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATg                                                                                                            >  1:761395/1‑140 (MQ=255)

               aaaCGGGTTTACTTCCGGCACCACCAGCGGTACGCCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATg                                                                                                            >  1:6228/1‑140 (MQ=255)

                 aCGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGcc                                                                                                          >  1:268479/1‑140 (MQ=255)

                    ggTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGc                                                                                                       <  1:344202/140‑1 (MQ=255)

                        tACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTgcg                                                                                                   >  1:488414/1‑140 (MQ=255)

                           ttCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCt                                                                                                <  2:761395/140‑1 (MQ=255)

                                gCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTc                                                                                           >  2:294279/1‑140 (MQ=255)

                                gCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTc                                                                                           <  1:655129/140‑1 (MQ=255)

                                   ccaccaGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAg                                                                                        <  2:560559/140‑1 (MQ=255)

                                               cGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCg                                                                            <  2:488414/140‑1 (MQ=255)

                                                    ggTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATc                                                                       <  2:718204/140‑1 (MQ=255)

                                                     gTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATcc                                                                      <  1:295145/140‑1 (MQ=255)

                                                       tCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTg                                                                    >  2:523799/1‑140 (MQ=255)

                                                         gagaGCAAACAATCCACTGCTGTCGATCGCCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCAGCGACAAAAAATGCCAGCTGCTCCTGCGTCCAGTCGAATTCAGCGGCATCCTGcg                                                                  <  2:6228/140‑2 (MQ=255)

                                                         gagaGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCa                                                                  <  1:294279/140‑1 (MQ=255)

                                                              cAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTg                                                             >  2:653410/1‑140 (MQ=255)

                                                                             tgTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGAtt                                                          >  1:662821/1‑128 (MQ=255)

                                                                             tgTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGAtt                                                          <  2:662821/128‑1 (MQ=255)

                                                                               tCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCaaa                                            >  2:173943/1‑140 (MQ=255)

                                                                                      ccAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAgt                                     >  1:411631/1‑140 (MQ=255)

                                                                                          gCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGTGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAgttgtt                                 <  1:653673/140‑1 (MQ=255)

                                                                                                  aGTTGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTgcg                         <  1:523799/140‑1 (MQ=255)

                                                                                                    ttGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCt                       >  2:873725/1‑140 (MQ=255)

                                                                                                     tGGTCGCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCtt                      >  1:518538/1‑140 (MQ=255)

                                                                                                          gCTTCTTCAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGtt                 >  2:362180/1‑140 (MQ=255)

                                                                                                             tcttcAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACg              <  2:268479/140‑1 (MQ=255)

                                                                                                                tcAACCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGc           <  1:653410/140‑1 (MQ=255)

                                                                                                                  aaCCCAGGCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCa         <  2:875433/140‑1 (MQ=255)

                                                                                                                        ggcagcggcagcTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCAt   >  2:772034/1‑140 (MQ=255)

                                                                                                                         gCAGCGGCAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATa  >  1:3659/1‑140 (MQ=255)

GTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCGGCATCCTGCACGGTGATTGTCTTACCACCAAAGCGCAGTTGTTCGCCTGCGCTTTCGTTACGTGCCAGTGCATA  >  NC_000913/2436287‑2436547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A