Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,458,416	T→C	E248E (GAA→GAG)	fadJ ←	3‑hydroxyacyl‑CoA dehydrogenase FadJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,458,416	0	T	C	100.0%	191.4 / NA	53	E248E (GAA→GAG)	fadJ	3‑hydroxyacyl‑CoA dehydrogenase FadJ
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/27); total (26/27)

CGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTC  >  NC_000913/2458281‑2458551

cGTACTGGCAAAAAAGATACTGCGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTg                                                                                                                                     >  2:381431/1‑140 (MQ=255)

  tACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTc                                                                                                                                   >  2:1287888/1‑140 (MQ=255)

  tACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTc                                                                                                                                   >  2:446048/1‑140 (MQ=255)

      ggCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCg                                                                                                                               <  1:446048/140‑1 (MQ=255)

         aaaaaaGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGAt                                                                                                                            >  1:273390/1‑140 (MQ=255)

            aaaGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAAt                                                                                                                         >  2:683728/1‑140 (MQ=255)

               gATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGc                                                                                                                      <  1:368203/140‑1 (MQ=255)

                aTACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGcc                                                                                                                     <  1:771895/140‑1 (MQ=255)

                  actacGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCtt                                                                                                                   <  2:988489/140‑1 (MQ=255)

                      cGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCt                                                                                                                    >  2:1010453/1‑135 (MQ=255)

                      cGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCt                                                                                                                    >  2:725042/1‑135 (MQ=255)

                      cGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCt                                                                                                                    <  1:725042/135‑1 (MQ=255)

                      cGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCt                                                                                                                    <  1:1010453/135‑1 (MQ=255)

                      cGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGt                                                                                                               >  1:1450318/1‑140 (MQ=255)

                      cGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGt                                                                                                               >  1:249445/1‑140 (MQ=255)

                       gCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGtt                                                                                                              >  1:1119542/1‑140 (MQ=255)

                         aGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGGTAATTGCCTTGAGtttt                                                                                                            >  1:1109466/1‑140 (MQ=255)

                         aGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGtttt                                                                                                            >  1:338008/1‑140 (MQ=255)

                           cgcCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTtgt                                                                                                          <  1:1287888/140‑1 (MQ=255)

                           cgcCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTtgt                                                                                                          >  1:1463340/1‑140 (MQ=255)

                            gcCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTtgtg                                                                                                         <  1:455034/140‑1 (MQ=255)

                             ccTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTgtgt                                                                                                        >  1:1087001/1‑140 (MQ=255)

                                gCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCt                                                                                                     >  1:653089/1‑140 (MQ=255)

                                       tgGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCt                                                                                              >  1:1090633/1‑140 (MQ=255)

                                          cGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGc                                                                                           <  1:683728/140‑1 (MQ=255)

                                             cATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGa                                                                                        <  1:1187406/140‑1 (MQ=255)

                                                                 cccGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCa                                                                    <  2:1450318/140‑1 (MQ=255)

                                                                     aGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGAc                                                                >  2:387186/1‑140 (MQ=255)

                                                                             cGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGc                                                        <  1:387186/140‑1 (MQ=255)

                                                                              gTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGcc                                                       <  1:606083/140‑1 (MQ=255)

                                                                              gTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGcc                                                       <  1:1368414/140‑1 (MQ=255)

                                                                                  tAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGc                                                   <  1:1016110/140‑1 (MQ=255)

                                                                                  tAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGc                                                   <  2:4553/140‑1 (MQ=255)

                                                                                         tgctgGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATa                                            <  2:653089/140‑1 (MQ=255)

                                                                                           ctgGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACg                                          >  2:1573885/1‑140 (MQ=255)

                                                                                           cgggTGCCCTGCGCTAATCCCGTTTCAACAACCTCCGGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCCCGACCGAACGGCCCCGCCAGAATACg                                          <  2:1401573/138‑1 (MQ=255)

                                                                                             ggTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCt                                        <  2:1087001/140‑1 (MQ=255)

                                                                                               tGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTcg                                      <  1:381431/140‑1 (MQ=255)

                                                                                                   cTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTa                                  <  2:1089687/140‑1 (MQ=255)

                                                                                                   cTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTa                                  <  1:1557911/140‑1 (MQ=255)

                                                                                                   cTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTa                                  <  2:1434506/140‑1 (MQ=255)

                                                                                                   cTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTa                                  <  1:330238/140‑1 (MQ=255)

                                                                                                     gcgcTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACa                                >  1:1584017/1‑140 (MQ=255)

                                                                                                     gcgcTAATCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACa                                >  1:1509827/1‑140 (MQ=255)

                                                                                                           aTCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTa                                  <  1:737044/132‑1 (MQ=255)

                                                                                                           aTCCCGTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTa                                  >  2:737044/1‑132 (MQ=255)

                                                                                                                gTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCg                     >  2:156521/1‑140 (MQ=255)

                                                                                                                gTTTCAACAACCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCg                     >  1:804281/1‑140 (MQ=255)

                                                                                                                     aacaccCTCCAGGATGCCCTCTGTCCCCGCATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGCCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAg                <  2:249445/140‑1 (MQ=255)

                                                                                                                       caacCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGc                      >  1:1107326/1‑132 (MQ=255)

                                                                                                                       caacCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGc                      <  2:1107326/132‑1 (MQ=255)

                                                                                                                        aacCTCCAGGATGCGCTCTGTCGCGGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATg             <  2:897726/140‑1 (MQ=255)

                                                                                                                        aacCTCCAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATg             <  1:1573885/140‑1 (MQ=255)

                                                                                                                              cAGGATGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCt       >  2:1026798/1‑140 (MQ=255)

                                                                                                                                   tGCGCTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCcttc  >  2:588281/1‑140 (MQ=255)

CGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTC  >  NC_000913/2458281‑2458551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A