Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,672,246	G→A	W67* (TGG→TAG)	hcaD →	putative 3‑phenylpropionate/cinnamate dioxygenase ferredoxin reductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,672,246	0	G	A	100.0%	233.6 / NA	65	W67* (TGG→TAG)	hcaD	putative 3‑phenylpropionate/cinnamate dioxygenase ferredoxin reductase subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (37/28); total (37/28)

GCTACGCCAGCAAGGGTTCACCGGTGAGCTGCATCTGTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAG  >  NC_000913/2672109‑2672380

gCTACGCCAGCAAGGGTTCACCGGTGAGCTGCATCTGTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACtagt                                                                                                                                      <  2:1335612/140‑1 (MQ=255)

          cAAGGGTTCACCGGTGAGCTGCATCTGTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGaaaa                                                                                                                            <  2:1713143/140‑1 (MQ=255)

                ttCACCGGTGAGCTGCATCTGTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGt                                                                                                                      >  1:626532/1‑140 (MQ=255)

                ttCACCGGTGAGCTGCATCTGTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGACAGGAAAACAATGt                                                                                                                      >  1:458220/1‑140 (MQ=255)

                   aCCGGTGAGCTGCATCTGTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCa                                                                                                                   >  1:62565/1‑140 (MQ=255)

                   aCCGGTGAGCTGCATCTGTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCa                                                                                                                   >  2:554085/1‑140 (MQ=255)

                    ccGGTGAGCTGCATCTGTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCAt                                                                                                                  <  2:626532/140‑1 (MQ=255)

                    ccGGTGAGCTGCATCTGTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGACAGGAAAACAATGTTCAt                                                                                                                  <  2:458220/140‑1 (MQ=255)

                      ggTGAGCTGCATCTGTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCt                                                                                                                <  2:1448311/140‑1 (MQ=255)

                      ggTGAGCTGCATCTGTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCt                                                                                                                <  2:62565/140‑1 (MQ=255)

                           gCTGCATCTGTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCAtt                                                                                                           <  2:332294/140‑1 (MQ=255)

                                 tCTGTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGgtg                                                                                                     >  1:465496/1‑140 (MQ=255)

                                    gTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTg                                                                                                               <  1:516176/127‑1 (MQ=255)

                                    gTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTg                                                                                                               >  2:516176/1‑127 (MQ=255)

                                          cGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCa                                                                                            <  1:509735/140‑1 (MQ=255)

                                               aGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTTGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACg                                                                                       >  1:98379/1‑140 (MQ=255)

                                                gCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGc                                                                                      <  2:465496/140‑1 (MQ=255)

                                                 cGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCt                                                                                     <  1:1547931/140‑1 (MQ=255)

                                                    cATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTggg                                                                                  >  1:700962/1‑140 (MQ=255)

                                                     aTCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGt                                                                                 <  2:59390/140‑1 (MQ=255)

                                                         tCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTcgcg                                                                             <  2:192035/140‑1 (MQ=255)

                                                          ccTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTg                                                                                    >  2:319525/1‑132 (MQ=255)

                                                          ccTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTg                                                                                    <  1:319525/132‑1 (MQ=255)

                                                          ccTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGa                                                                            <  1:554085/140‑1 (MQ=255)

                                                              aTGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGacaca                                                                        >  2:293165/1‑140 (MQ=255)

                                                                gAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACg                                                                      <  2:1297349/140‑1 (MQ=255)

                                                                gAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACg                                                                      <  2:700962/140‑1 (MQ=255)

                                                                    gACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGagag                                                                  >  2:821772/1‑140 (MQ=255)

                                                                     aCCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACg                                                                      <  1:285131/135‑1 (MQ=255)

                                                                     aCCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACg                                                                      >  2:285131/1‑135 (MQ=255)

                                                                     aCCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGt                                                                 <  2:725670/140‑1 (MQ=255)

                                                                      ccTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGtt                                                                >  1:65382/1‑140 (MQ=255)

                                                                            ctctCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGtt                                                          >  2:1458180/1‑140 (MQ=255)

                                                                               tcGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAAc                                                       >  2:120574/1‑140 (MQ=255)

                                                                                    aTCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACg                                                  >  2:276551/1‑140 (MQ=255)

                                                                                      ccATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGc                                                >  2:1167100/1‑140 (MQ=255)

                                                                                       cATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCg                                               <  2:1641466/140‑1 (MQ=255)

                                                                                       cATGTTGCTGGAAGATTCCCCACAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCg                                               <  2:906819/140‑1 (MQ=255)

                                                                                        aTGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGa                                              <  1:988428/140‑1 (MQ=255)

                                                                                         tGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGaa                                             >  1:1307859/1‑140 (MQ=255)

                                                                                           ttGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAg                                           <  1:1458180/140‑1 (MQ=255)

                                                                                            tGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGc                                          <  2:1307859/140‑1 (MQ=255)

                                                                                            tGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGc                                          <  1:276551/140‑1 (MQ=255)

                                                                                              cTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTg                                        <  1:1167100/140‑1 (MQ=255)

                                                                                                  aaGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCAc                                    <  1:821772/140‑1 (MQ=255)

                                                                                                  aaGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGGTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCAc                                    <  2:722104/140‑1 (MQ=255)

                                                                                                     aTTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTgg                                 >  2:282872/1‑140 (MQ=255)

                                                                                                      ttCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTggg                                >  1:7840/1‑140 (MQ=255)

                                                                                                      ttCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTggg                                >  1:547901/1‑140 (MQ=255)

                                                                                                        ccccGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGAt                              >  1:609008/1‑140 (MQ=255)

                                                                                                        ccccGCAGTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGAt                              <  2:7840/140‑1 (MQ=255)

                                                                                                               gTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCttt                       >  2:1276166/1‑140 (MQ=255)

                                                                                                               gTTACAGCAGGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCttt                       >  2:353089/1‑140 (MQ=255)

                                                                                                                       agGTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCa               <  1:293165/140‑1 (MQ=255)

                                                                                                                        ggTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCaa              >  1:1517256/1‑140 (MQ=255)

                                                                                                                        ggTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCaa              >  1:30121/1‑140 (MQ=255)

                                                                                                                        ggTGTTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCaa              >  1:771482/1‑140 (MQ=255)

                                                                                                                          tgtTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAAcc            >  2:1187564/1‑140 (MQ=255)

                                                                                                                          tgtTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAAcc            >  1:945600/1‑140 (MQ=255)

                                                                                                                          tgtTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAAcc            >  1:1645038/1‑140 (MQ=255)

                                                                                                                          tgtTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAAcc            >  1:1187424/1‑140 (MQ=255)

                                                                                                                          tgtTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGGCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAAcc            >  1:739421/1‑140 (MQ=255)

                                                                                                                          tgtTACCCGCTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAAcc            >  2:730452/1‑140 (MQ=255)

                                                                                                                                   cTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCa   >  2:585592/1‑140 (MQ=255)

                                                                                                                                   cTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCa   >  2:191609/1‑140 (MQ=255)

                                                                                                                                   cTAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCa   >  2:1116921/1‑140 (MQ=255)

                                                                                                                                    tAACTAGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAg  >  1:1348593/1‑140 (MQ=255)

GCTACGCCAGCAAGGGTTCACCGGTGAGCTGCATCTGTTTTCCGATGAGCGACATCTTCCTTATGAACGACCTCCACTCTCGAAATCCATGTTGCTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAG  >  NC_000913/2672109‑2672380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A