Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,673,740	C→T	P132L (CCG→CTG)	yphA →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,673,740	0	C	T	100.0%	151.3 / NA	44	P132L (CCG→CTG)	yphA	putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/22); total (22/22)

TGGGTACGGCGGTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGC  >  NC_000913/2673614‑2673875

tGGGTACGGCGGTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTtct                                                                                                                            <  2:1135537/140‑1 (MQ=255)

     aCGGCGGTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGa                                                                                                                       <  1:117310/140‑1 (MQ=255)

     aCGGCGGTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGa                                                                                                                       <  1:12549/140‑1 (MQ=255)

      cggcggTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGAt                                                                                                                      <  1:754354/140‑1 (MQ=255)

       ggcggTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCTATc                                                                                                                     <  2:215983/140‑1 (MQ=255)

           gTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCg                                                                                                                 <  1:903497/140‑1 (MQ=255)

           gTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCg                                                                                                                 <  1:731326/140‑1 (MQ=255)

                   tCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGaaa                                                                                                         >  2:24419/1‑140 (MQ=255)

                            cTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGa                                                                                                <  1:663176/140‑1 (MQ=255)

                                 atatGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATgcgg                                                                                           >  1:276243/1‑140 (MQ=255)

                                 atatGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATgcgg                                                                                           >  2:522956/1‑140 (MQ=255)

                                    tGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGt                                                                                        >  2:49560/1‑140 (MQ=255)

                                    tGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGt                                                                                        >  2:1014424/1‑140 (MQ=255)

                                      aCCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGa                                                                                      >  2:165370/1‑140 (MQ=255)

                                          gCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATgcg                                                                                            <  2:44670/130‑1 (MQ=255)

                                          gCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATgcg                                                                                            >  1:44670/1‑130 (MQ=255)

                                           cGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGcc                                                                                 <  1:356625/140‑1 (MQ=255)

                                              tGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTa                                                                              >  1:1216255/1‑140 (MQ=255)

                                                cGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTg                                                                                       <  2:865011/129‑1 (MQ=255)

                                                cGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTg                                                                                       >  1:865011/1‑129 (MQ=255)

                                                          aaaTATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaa                                                                  <  2:1216255/140‑1 (MQ=255)

                                                                 aTTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTa                                                           >  2:1162240/1‑140 (MQ=255)

                                                                     aTTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                       <  1:1192450/140‑1 (MQ=255)

                                                                      tttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGcc                                                      >  1:518171/1‑140 (MQ=255)

                                                                       ttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCa                                                     >  2:1204148/1‑140 (MQ=255)

                                                                            gaagaaTGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTa                                                <  2:518171/140‑1 (MQ=255)

                                                                                  tGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCa                                          <  2:276243/140‑1 (MQ=255)

                                                                                          tCGCTGGCGCGTTCTTGCTATTGGCAATTACCAGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACAtc                                  >  1:323896/1‑140 (MQ=255)

                                                                                              tGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTg                              >  2:27699/1‑140 (MQ=255)

                                                                                                gcgcGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGa                                      >  2:249166/1‑130 (MQ=255)

                                                                                                gcgcGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGa                                      <  1:249166/130‑1 (MQ=255)

                                                                                                 cgcgTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAg                           <  1:847644/140‑1 (MQ=255)

                                                                                                     ttCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGa                                      <  2:1003574/125‑1 (MQ=255)

                                                                                                     ttCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGa                                      >  1:1003574/1‑125 (MQ=255)

                                                                                                        ttGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGa                    <  1:522956/140‑1 (MQ=255)

                                                                                                        ttGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGa                    <  1:165370/140‑1 (MQ=255)

                                                                                                               tGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCt             >  2:817522/1‑140 (MQ=255)

                                                                                                                ggCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTg            >  1:506518/1‑140 (MQ=255)

                                                                                                                ggCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTg            >  2:363455/1‑140 (MQ=255)

                                                                                                                ggCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTg            >  1:22155/1‑140 (MQ=255)

                                                                                                                ggCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTg            >  1:131059/1‑140 (MQ=255)

                                                                                                                  cAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGa                    >  2:1090646/1‑130 (MQ=255)

                                                                                                                  cAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGa                    <  1:1090646/130‑1 (MQ=255)

                                                                                                                      tACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTa      <  1:24419/140‑1 (MQ=255)

                                                                                                                        ccgggctgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgc    >  1:162469/1‑140 (MQ=255)

                                                                                                                        ccgggctgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgc    >  2:576453/1‑140 (MQ=255)

                                                                                                                          gggctgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgcgc  <  1:363455/140‑1 (MQ=255)

TGGGTACGGCGGTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGC  >  NC_000913/2673614‑2673875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A