Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,715,394	A→G	intergenic (‑74/‑29)	yfiE ← / → eamB	putative LysR‑type DNA‑binding transcriptional regulator YfiE/cysteine/O‑acetylserine exporter EamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,715,394	0	A	G	98.6%	255.4 / ‑5.2	72	intergenic (‑74/‑29)	yfiE/eamB	putative LysR‑type DNA‑binding transcriptional regulator YfiE/cysteine/O‑acetylserine exporter EamB
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (30/41); total (31/41)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTT  >  NC_000913/2715258‑2715530

aGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTg                                                                                                                                       <  2:240769/140‑1 (MQ=255)

  cTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTCTAAATGTTCCGTGTCGGGTGCTGTc                                                                                                                                     >  2:185150/1‑140 (MQ=255)

     gCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTAc                                                                                                                                  <  1:482971/140‑1 (MQ=255)

            ggAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACa                                                                                                                           <  2:640048/140‑1 (MQ=255)

               aCCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACagag                                                                                                                        <  2:931813/140‑1 (MQ=255)

               aCCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACagag                                                                                                                        >  2:1460451/1‑140 (MQ=255)

               aCCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACagag                                                                                                                        <  2:123721/140‑1 (MQ=255)

               aCCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACa                                                                                                                           >  1:387607/1‑137 (MQ=255)

               aCCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACa                                                                                                                           <  2:387607/137‑1 (MQ=255)

               aCCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTCTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACagag                                                                                                                        <  1:185150/140‑1 (MQ=255)

                   tctTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGgaga                                                                                                                    >  2:308773/1‑140 (MQ=255)

                               gTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGaca                                                                                                        <  2:1702938/140‑1 (MQ=255)

                                  ttAAGCGTAATAAAACGGTGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCg                                                                                                     <  1:1640165/140‑1 (MQ=255)

                                  ttAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCg                                                                                                     <  1:788472/140‑1 (MQ=255)

                                  ttAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCg                                                                                                     <  2:85852/140‑1 (MQ=255)

                                  ttAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCg                                                                                                     <  2:717000/140‑1 (MQ=255)

                                                  ggCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCtt                                                                                     >  2:63773/1‑140 (MQ=255)

                                                    cgcAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCtttt                                                                                   >  2:1545585/1‑140 (MQ=255)

                                                      cAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTg                                                                                 <  2:273836/140‑1 (MQ=255)

                                                      cAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTAACACCGACCCTTTTAAGTGCTTTTTg                                                                                 <  1:308773/140‑1 (MQ=255)

                                                             aTAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTac                                                                          <  1:1460451/140‑1 (MQ=255)

                                                              tAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTaca                                                                         >  1:448941/1‑140 (MQ=255)

                                                              tAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTaca                                                                         >  1:108348/1‑140 (MQ=255)

                                                                   ccAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTg                                                                    >  1:423306/1‑140 (MQ=255)

                                                                    cAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACtt                                                                            >  1:415531/1‑131 (MQ=255)

                                                                    cAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACtt                                                                            <  2:415531/131‑1 (MQ=255)

                                                                    cAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGa                                                                   >  2:1027045/1‑140 (MQ=255)

                                                                     aaaCCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGAt                                                                  >  1:1260051/1‑140 (MQ=255)

                                                                     aaaCCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGAt                                                                  >  2:637292/1‑140 (MQ=255)

                                                                     aaaCCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGAt                                                                  <  2:461376/140‑1 (MQ=255)

                                                                      aaCCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTacac                                                                        <  1:1124640/133‑1 (MQ=255)

                                                                      aaCCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTacac                                                                        >  2:1124640/1‑133 (MQ=255)

                                                                      aaCCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTa                                                                           <  2:711659/130‑1 (MQ=255)

                                                                      aaCCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTa                                                                           >  1:711659/1‑130 (MQ=255)

                                                                      aaCCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGAtt                                                                 >  1:1131819/1‑140 (MQ=255)

                                                                         cTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTAcc                                                              >  2:1309612/1‑140 (MQ=255)

                                                                           aTCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGc                                                            >  2:1164991/1‑140 (MQ=255)

                                                                           aTCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGc                                                            >  1:424877/1‑140 (MQ=255)

                                                                            tCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCt                                                           >  1:195982/1‑140 (MQ=255)

                                                                            tCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCt                                                           >  1:1241910/1‑140 (MQ=255)

                                                                            tCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCt                                                           >  2:925883/1‑140 (MQ=255)

                                                                            tCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCt                                                           >  2:1527707/1‑140 (MQ=255)

                                                                            tCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGCCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGATTTTTGGACTTACACCCTGATTACCGCt                                                           >  1:540881/1‑140 (MQ=255)

                                                                              gAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTAt                                                         <  2:448941/140‑1 (MQ=255)

                                                                              gAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTAt                                                         <  1:1309612/140‑1 (MQ=255)

                                                                                   tatCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGc                                                    <  1:392962/140‑1 (MQ=255)

                                                                                     tCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCa                                                  <  2:913935/140‑1 (MQ=255)

                                                                                      cGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAg                                                 >  2:670723/1‑140 (MQ=255)

                                                                                        aaTCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGa                                               <  2:574425/140‑1 (MQ=255)

                                                                                        aaTCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGa                                               <  1:925883/140‑1 (MQ=255)

                                                                                        aaTCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGa                                               <  1:637292/140‑1 (MQ=255)

                                                                                        aaTCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGa                                               <  1:1527707/140‑1 (MQ=255)

                                                                                        aaTCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGa                                               <  2:423306/140‑1 (MQ=255)

                                                                                          tCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGAcc                                             <  1:63773/140‑1 (MQ=255)

                                                                                           cTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCg                                            <  2:1131819/140‑1 (MQ=255)

                                                                                             aGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGc                                                    >  2:957834/1‑130 (MQ=255)

                                                                                             aGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGc                                                    <  1:957834/130‑1 (MQ=255)

                                                                                             aGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGAc                                              <  2:684271/136‑1 (MQ=255)

                                                                                             aGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGAc                                              >  1:684271/1‑136 (MQ=255)

                                                                                                   taAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAatat                                    >  1:518860/1‑140 (MQ=255)

                                                                                                       aaCATTCATTTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATtctc                                <  1:1027045/140‑1 (MQ=255)

                                                                                                          attcattTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGcc                             <  2:1260051/140‑1 (MQ=255)

                                                                                                          attcattTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGcc                             <  1:1164991/140‑1 (MQ=255)

                                                                                                           ttcattTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGccc                            >  2:1537265/1‑140 (MQ=255)

                                                                                                           ttcattTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGccc                            >  2:381932/1‑140 (MQ=255)

                                                                                                           ttcattTTTTTAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGccc                            >  1:1568017/1‑140 (MQ=255)

                                                                                                               tttttttAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAg                        <  1:670723/140‑1 (MQ=255)

                                                                                                               tttttttAAATGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAg                        <  1:351406/140‑1 (MQ=255)

                                                                                                                        aTGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTAc               <  1:1027329/140‑1 (MQ=255)

                                                                                                                         tGTTCCGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACg              <  1:381932/140‑1 (MQ=255)

                                                                                                                              cGTGTCGGGTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCa         <  2:518860/140‑1 (MQ=255)

                                                                                                                                tgtCGGGGGCTGTCCACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCacg       >  2:86926/1‑138 (MQ=255)

                                                                                                                                     ggTGCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGAttt  <  2:424877/140‑1 (MQ=255)

AGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTT  >  NC_000913/2715258‑2715530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A