Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 180 66.5 2263572 99.4% 2249990 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,715,501 G→A A27T (GCC→ACC)  eamB → cysteine/O‑acetylserine exporter EamB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,715,5010GA98.1% 169.5 / ‑4.3 53A27T (GCC→ACC) eamBcysteine/O‑acetylserine exporter EamB
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (27/25);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

TTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTGATTGACCCGGC  >  NC_000913/2715365‑2715631
                                                                                                                                        |                                                                                                                                  
ttcattTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCAccc                                                                                                                                 >  1:1129962/1‑140 (MQ=255)
ttcattTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCAccc                                                                                                                                 >  1:192333/1‑140 (MQ=255)
  cattTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCtt                                                                                                                               <  2:192333/140‑1 (MQ=255)
    tttttttAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAg                                                                                                                             <  1:1069485/140‑1 (MQ=255)
    tttttttAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAg                                                                                                                             <  2:249483/140‑1 (MQ=255)
                 tCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCg                                                                                                                >  1:765537/1‑140 (MQ=255)
                        cGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGAt                                                                                                         <  1:426663/140‑1 (MQ=255)
                               tGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATGTTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCa                                                                                                  >  1:948464/1‑140 (MQ=255)
                               tGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTGGTCa                                                                                                  >  2:1041167/1‑140 (MQ=255)
                               tGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCa                                                                                                  <  2:394411/140‑1 (MQ=255)
                                    aCCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTa                                                                                             >  1:271774/1‑140 (MQ=255)
                                      cAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTAcc                                                                                           <  2:244303/140‑1 (MQ=255)
                                             gaggagATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGc                                                                                    >  2:1043884/1‑140 (MQ=255)
                                                gagaTAACAAGTGACACCGTCCCTTTTAAGTGCTTTTTGGGCTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTgg                                                                                 >  1:359794/1‑140 (MQ=255)
                                                 agaTAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGc                                                                                <  2:1129962/140‑1 (MQ=255)
                                                   aTAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAg                                                                              >  2:878079/1‑140 (MQ=255)
                                                       cAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATGTTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGAt                                                                          <  2:948464/140‑1 (MQ=255)
                                                          gTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAg                                                                       >  1:683052/1‑140 (MQ=255)
                                                            gACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTc                                                                     >  1:945879/1‑140 (MQ=255)
                                                             acacCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCt                                                                    >  2:653644/1‑140 (MQ=255)
                                                             acacCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCt                                                                    >  1:740298/1‑140 (MQ=255)
                                                             acacCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCt                                                                    >  1:1008105/1‑140 (MQ=255)
                                                                ccGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTggg                                                                 <  2:945879/140‑1 (MQ=255)
                                                                  gACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGAt                                                               >  1:328793/1‑140 (MQ=255)
                                                                  gACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGAt                                                               >  1:945419/1‑140 (MQ=255)
                                                                   aCCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGAtt                                                              >  2:145122/1‑140 (MQ=255)
                                                                   aCCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGAtt                                                              >  1:972464/1‑140 (MQ=255)
                                                                    cccTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGAttt                                                             >  1:1038214/1‑140 (MQ=255)
                                                                         ttAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGAt                                                        >  2:240597/1‑140 (MQ=255)
                                                                           aaGTGCTTTTTGGGCTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATtg                                                      <  2:359794/140‑1 (MQ=255)
                                                                           aaGTGCTTTTTGGACTTCCACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCACAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATtg                                                      <  2:1008105/140‑1 (MQ=255)
                                                                           aaGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATtg                                                      <  2:13316/140‑1 (MQ=255)
                                                                           aaGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATtg                                                      <  1:1043884/140‑1 (MQ=255)
                                                                           aaGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATtg                                                      <  2:945419/140‑1 (MQ=255)
                                                                           aaGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATtg                                                      <  1:653644/140‑1 (MQ=255)
                                                                           aaGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGAATGAGTCTGGGATTTTTGATtg                                                      <  2:765537/140‑1 (MQ=255)
                                                                            aGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATtgt                                                     <  2:279462/140‑1 (MQ=255)
                                                                             gTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATtgtg                                                    <  2:972464/140‑1 (MQ=255)
                                                                                  ttttGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGtt                                               <  1:912094/140‑1 (MQ=255)
                                                                                               cccTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGc                                  <  2:314454/140‑1 (MQ=255)
                                                                                                 cTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGcat                                >  1:295769/1‑140 (MQ=255)
                                                                                                    aTTACCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGcatttc                             >  2:20853/1‑140 (MQ=255)
                                                                                                       aCCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGcatttcatt                          <  2:271774/140‑1 (MQ=255)
                                                                                                       aCCGCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGcatttcatt                          >  1:905453/1‑140 (MQ=255)
                                                                                                          gCTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTc                       >  1:652182/1‑140 (MQ=255)
                                                                                                           cTATGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCa                      <  1:878079/140‑1 (MQ=255)
                                                                                                             aTGACGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACt                    <  1:145122/140‑1 (MQ=255)
                                                                                                                aCGCCAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGc                 <  2:735681/140‑1 (MQ=255)
                                                                                                                   ccAGGACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGt              >  2:391410/1‑140 (MQ=255)
                                                                                                                      ggACCGAACAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTGAt           >  1:283192/1‑140 (MQ=255)
                                                                                                                            aaCAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTGATTGAccc     >  1:553817/1‑140 (MQ=255)
                                                                                                                            aaCAATATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTGATTGAccc     >  1:784647/1‑140 (MQ=255)
                                                                                                                               aaTATTCTCACCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTGATTGACCCGGc  <  2:652182/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                  
TTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCAAAGTACCCGCGTGCTGGCAGGGATGAGTCTGGGATTTTTGATTGTGATGTTACTGTGTGCGGGCATTTCATTTTCACTGGCAGTGATTGACCCGGC  >  NC_000913/2715365‑2715631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A