Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 183 98.6 3427438 96.9% 3321187 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,138,494 Δ1 bp coding (29/1860 nt) gss ← fused glutathionylspermidine amidase/glutathionylspermidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,138,4910G.99.2% 571.4 / ‑2.7 133coding (32/1860 nt)gssfused glutathionylspermidine amidase/glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (68/64);  total (69/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 138 total aligned reads displayed.

CACTTGTGGCCCATATATTCGTCGTCGATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAACGGGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTCAGACTAATCATACAC  >  NC_000913/3138353‑3138625
                                                                                                                                          |                                                                                                                                      
cACTTGTGGCCCATATATTCGTCGTCGATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAACgg                                                                                                                                       <  2:1123008/140‑1 (MQ=255)
     gtgGCCCATATATTCGTCGTCGATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAACgggcatc                                                                                                                                  >  1:559524/1‑136 (MQ=255)
           cATATATTCGTCGTCGATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCctggct                                                                                                                           <  1:966574/140‑1 (MQ=255)
             tatatTCGTCGTCGATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCctggctgg                                                                                                                         >  1:848782/1‑140 (MQ=255)
                  tcgtcgtcgATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGtt                                                                                                                    >  2:550616/1‑140 (MQ=255)
                     tcgtcgATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCt                                                                                                                 <  1:491138/140‑1 (MQ=255)
                       gtcgATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGt                                                                                                                        <  1:1057166/131‑1 (MQ=255)
                       gtcgATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGt                                                                                                                        >  2:1057166/1‑131 (MQ=255)
                       gtcgATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCttt                                                                                                               >  2:328200/1‑140 (MQ=255)
                        tcgATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTg                                                                                                              >  2:1052541/1‑140 (MQ=255)
                        tcgATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTg                                                                                                              >  1:605809/1‑140 (MQ=255)
                        tcgATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTg                                                                                                              >  2:1635018/1‑140 (MQ=255)
                         cgATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGTGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGc                                                                                                             >  1:227357/1‑140 (MQ=255)
                           atatAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCtc                                                                                                           <  1:739714/140‑1 (MQ=255)
                             ataGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCtcat                                                                                                         <  2:718468/140‑1 (MQ=255)
                                    cGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTa                                                                                                  >  2:47906/1‑140 (MQ=255)
                                      gAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCtc                                                                                                           >  1:867657/1‑129 (MQ=255)
                                      gAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCtc                                                                                                           >  2:1767/1‑129 (MQ=255)
                                      gAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTAcc                                                                                                <  1:280896/140‑1 (MQ=255)
                                        aTACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCtt                                                                                              <  1:1453527/140‑1 (MQ=255)
                                         tACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑TGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCttt                                                                                             >  1:945738/1‑140 (MQ=255)
                                         tACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCttt                                                                                             <  2:55474/140‑1 (MQ=255)
                                          aCGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTa                                                                                            <  1:1593916/140‑1 (MQ=255)
                                              cGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACaa                                                                                        >  1:209806/1‑140 (MQ=255)
                                              cGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGT‑GCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAAt                                                                                       >  2:860948/1‑140 (MQ=255)
                                               gTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAAt                                                                                       <  1:1701356/140‑1 (MQ=255)
                                                 cATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACt                                                                                                     >  2:1641789/1‑124 (MQ=255)
                                                 cATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATAc                                                                                     <  2:334792/140‑1 (MQ=255)
                                                 cATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATAc                                                                                     >  2:1316627/1‑140 (MQ=255)
                                                   tCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACaa                                                                                   >  2:402244/1‑140 (MQ=255)
                                                   tCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACaa                                                                                   >  1:725533/1‑140 (MQ=255)
                                                             ccTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAACGGGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAg                                                                          >  1:174220/1‑140 (MQ=255)
                                                             ccTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGt                                                                         <  2:605809/140‑1 (MQ=255)
                                                             ccTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGt                                                                         >  1:591695/1‑140 (MQ=255)
                                                             ccTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGt                                                                         <  1:1316627/140‑1 (MQ=255)
                                                             ccTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGt                                                                         <  1:641833/140‑1 (MQ=255)
                                                                gCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTgg                                                                      <  2:673490/140‑1 (MQ=255)
                                                                 cGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTgg                                                                      <  2:778044/139‑1 (MQ=255)
                                                                 cGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGc                                                                     >  2:1333953/1‑140 (MQ=255)
                                                                 cGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGc                                                                     >  2:1492387/1‑140 (MQ=255)
                                                                   gATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGt                                                                   <  2:978437/140‑1 (MQ=255)
                                                                    aTCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTa                                                                  >  1:573095/1‑140 (MQ=255)
                                                                     tCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTaa                                                                 >  2:288849/1‑140 (MQ=255)
                                                                      cGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAAt                                                                >  2:1167214/1‑140 (MQ=255)
                                                                          agaACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAAttgtt                                                            <  2:872233/140‑1 (MQ=255)
                                                                             aCTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTtgtg                                                         <  1:1635018/140‑1 (MQ=255)
                                                                             aCTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTtgtg                                                         <  1:288849/140‑1 (MQ=255)
                                                                                gTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGctc                                                      <  1:637221/140‑1 (MQ=255)
                                                                                  aaTCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTg                                                    <  2:559524/140‑1 (MQ=255)
                                                                                         agagTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGTGTAATTGTTGTGCTCTGATTACCt                                             >  1:1515646/1‑140 (MQ=255)
                                                                                             tAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTc                                         >  2:776475/1‑140 (MQ=255)
                                                                                               gATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAg                                       >  2:804432/1‑140 (MQ=255)
                                                                                               gATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAg                                       <  1:47906/140‑1 (MQ=255)
                                                                                                aTTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGc                                      >  2:631012/1‑140 (MQ=255)
                                                                                                 ttGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCg                                     <  2:9221/140‑1 (MQ=255)
                                                                                                 ttGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCg                                     <  1:1167214/140‑1 (MQ=255)
                                                                                                     tACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACAt                                 >  2:939446/1‑140 (MQ=255)
                                                                                                     tACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACAt                                 <  1:328200/140‑1 (MQ=255)
                                                                                                     tACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACAt                                 >  1:1666071/1‑140 (MQ=255)
                                                                                                     tACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTAATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACAt                                 >  1:761492/1‑140 (MQ=255)
                                                                                                       ccccACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTa                               >  1:688448/1‑140 (MQ=255)
                                                                                                       ccccACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTa                               >  2:789235/1‑140 (MQ=255)
                                                                                                       ccccACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTa                               >  2:325738/1‑140 (MQ=255)
                                                                                                       ccccACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTa                               >  1:1646354/1‑140 (MQ=255)
                                                                                                       ccccACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTa                               >  1:626692/1‑140 (MQ=255)
                                                                                                       ccccACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTAATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTa                               <  2:761492/140‑1 (MQ=255)
                                                                                                        cccACCTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCTTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTaa                              <  2:1056285/140‑1 (MQ=255)
                                                                                                            ccTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAg                                       >  1:620388/1‑127 (MQ=255)
                                                                                                            ccTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAg                                       <  2:620388/127‑1 (MQ=255)
                                                                                                            ccTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCgg                          >  1:1468796/1‑140 (MQ=255)
                                                                                                             cTGGGGCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGa                         >  1:1083492/1‑140 (MQ=255)
                                                                                                               ggggCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACa                       <  2:725533/140‑1 (MQ=255)
                                                                                                               ggggCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACa                       <  2:1468796/140‑1 (MQ=255)
                                                                                                               ggggCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACa                       <  2:469773/140‑1 (MQ=255)
                                                                                                                 ggCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACagg                     >  1:1033917/1‑139 (MQ=255)
                                                                                                                 ggCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACaga                     >  1:86254/1‑140 (MQ=255)
                                                                                                                 ggCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTCCTCTGATTACCTGCTCAGCGACATTAACCGGACagt                     >  1:1403942/1‑139 (MQ=255)
                                                                                                                  gCGTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACagag                    <  1:776475/140‑1 (MQ=255)
                                                                                                                    gTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGt                  <  1:1234674/140‑1 (MQ=255)
                                                                                                                    gTAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGt                  <  1:778582/140‑1 (MQ=255)
                                                                                                                     tAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACa                       >  2:1533123/1‑134 (MQ=255)
                                                                                                                     tAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTc                 >  2:1304945/1‑140 (MQ=255)
                                                                                                                     tAGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTc                 >  2:847667/1‑140 (MQ=255)
                                                                                                                      aGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTCa                <  1:847667/140‑1 (MQ=255)
                                                                                                                      aGCCCAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTCa                >  1:1696936/1‑140 (MQ=255)
                                                                                                                         ccAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACTTGCTCAGCGACATTAACCGGACAGAGGTCAGAc             <  1:402244/140‑1 (MQ=255)
                                                                                                                         ccAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTCAGAc             <  2:688448/140‑1 (MQ=255)
                                                                                                                         ccAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTCAGAc             <  2:227357/140‑1 (MQ=255)
                                                                                                                         ccAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTCAGAc             <  2:86254/140‑1 (MQ=255)
                                                                                                                          cAATAATGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTCAGACt            <  1:1435610/140‑1 (MQ=255)
                                                                                                                           aataatGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTCAGACTa           >  1:1251621/1‑140 (MQ=255)
                                                                                                                           aataatGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTCAGACTa           >  1:1646253/1‑140 (MQ=255)
                                                                                                                            ataatGTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTCAGACTaa          <  2:591695/140‑1 (MQ=255)
                                                                                                                                 gTCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGACTACCTGCTCAGCGACATTAACCGGACAGAGGTCAGACTAATCATa     <  2:1666071/140‑1 (MQ=255)
                                                                                                                                  tCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTCAGACTAATCATac    >  1:505917/1‑140 (MQ=255)
                                                                                                                                  tCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTCAGACTAATCATac    >  1:1217104/1‑140 (MQ=255)
                                                                                                                                  tCCCGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTCAGACTAATCATac    >  1:1134560/1‑140 (MQ=255)
                                                                                                                                   cccGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTCAGACTAATCATa     <  1:432365/138‑1 (MQ=255)
                                                                                                                                     cGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGt                  <  1:956424/123‑1 (MQ=255)
                                                                                                                                     cGAAC‑GGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGt                  >  2:956424/1‑123 (MQ=255)
                                                                                                                                          |                                                                                                                                      
CACTTGTGGCCCATATATTCGTCGTCGATATAGCTACGGAATACGGCGTCATCTTCGTATTCCTGCGGATCGAGAGAACTGTAATCTGAAGAGTAGATTGCTACCCCACCTGGGGCGTAGCCCAATAATGTCCCGAACGGGGCATCCTGGCTGGTCGTTCCTTTGCTCATCACTTTACCTTTAACAATACAACCTAAGCAGTTGGCGTAATTGTTGTGCTCTGATTACCTGCTCAGCGACATTAACCGGACAGAGGTCAGACTAATCATACAC  >  NC_000913/3138353‑3138625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A