Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 183 98.6 3427438 96.9% 3321187 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,495,416 T→C V469A (GTA→GCA)  nirB → nitrite reductase catalytic subunit NirB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,495,4160TC100.0% 272.2 / NA 73V469A (GTA→GCA) nirBnitrite reductase catalytic subunit NirB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/37);  total (36/37)

CTCCTGCTTCGACGTCACCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGA  >  NC_000913/3495285‑3495550
                                                                                                                                   |                                                                                                                                      
cTCCTGCTTCGACGTCACCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAAcg                                                                                                                                <  2:1429365/140‑1 (MQ=255)
cTCCTGCTTCGACGTCACCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAAcg                                                                                                                                <  1:1141462/140‑1 (MQ=255)
    tGCTTCGACGTCACCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGa                                                                                                                            <  2:84075/140‑1 (MQ=255)
     gCTTCGACGTCACCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGaa                                                                                                                           >  1:866734/1‑140 (MQ=255)
     gCTTCGACGTCACCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGaa                                                                                                                           <  1:1315881/140‑1 (MQ=255)
      cTTCGACGTCACCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAAc                                                                                                                          <  1:917079/140‑1 (MQ=255)
         cGACGTCACCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTgg                                                                                                                       <  1:1228423/140‑1 (MQ=255)
          gACGTCACCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGc                                                                                                                      >  1:628169/1‑140 (MQ=255)
          gACGTCACCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGc                                                                                                                      >  2:13508/1‑140 (MQ=255)
             gTCACCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGaa                                                                                                                   >  1:556084/1‑140 (MQ=255)
              tCACCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCAGACAGTTGCGGCGCTGAAAGCTGAAGCCAAAGCGGGTGCTGGCTGCGGTGGCTGTATCACGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGaaa                                                                                                                  <  2:694994/140‑1 (MQ=255)
               cACCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAAc                                                                                                                 >  2:1389557/1‑140 (MQ=255)
                aCCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACa                                                                                                                <  1:13508/140‑1 (MQ=255)
                aCCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACa                                                                                                                <  1:15368/140‑1 (MQ=255)
                 ccAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAg                                                                                                               <  1:1577904/140‑1 (MQ=255)
                        tGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCg                                                                                                        <  2:882508/140‑1 (MQ=255)
                        tGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCg                                                                                                        <  2:587814/140‑1 (MQ=255)
                          aTCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGaa                                                                                                      >  2:1010681/1‑140 (MQ=255)
                          aTCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGaa                                                                                                      >  1:3491/1‑140 (MQ=255)
                            cTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGt                                                                                                    >  1:711531/1‑140 (MQ=255)
                            cTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGt                                                                                                    <  2:68281/140‑1 (MQ=255)
                            cTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGt                                                                                                    <  1:956135/140‑1 (MQ=255)
                               aTTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTaa                                                                                                 >  2:1489573/1‑140 (MQ=255)
                               aTTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTaa                                                                                                 >  1:742667/1‑140 (MQ=255)
                                ttGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTaac                                                                                                >  2:238543/1‑140 (MQ=255)
                                 tgctgcCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTaaca                                                                                               >  2:281232/1‑140 (MQ=255)
                                     gcCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTaacaacaa                                                                                           >  2:1688041/1‑140 (MQ=255)
                                      ccATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGt                                                                                                    >  1:465539/1‑130 (MQ=255)
                                      ccATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGt                                                                                                    <  2:465539/130‑1 (MQ=255)
                                      ccATCAACAAAGGCTGCCACACAGCTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTaacaacaac                                                                                          >  1:1673234/1‑140 (MQ=255)
                                       cATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAAcc                                                                                         <  2:3491/140‑1 (MQ=255)
                                       cATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAAcc                                                                                         <  2:866734/140‑1 (MQ=255)
                                         tCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCtg                                                                                       >  1:849537/1‑140 (MQ=255)
                                               aaGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGaa                                                                                 <  1:1127675/140‑1 (MQ=255)
                                                aGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAAcc                                                                                         >  2:1537298/1‑131 (MQ=255)
                                                aGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAAcc                                                                                         >  1:1326245/1‑131 (MQ=255)
                                                aGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAAcc                                                                                         <  1:1537298/131‑1 (MQ=255)
                                                aGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAAcc                                                                                         <  2:1326245/131‑1 (MQ=255)
                                                aGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAac                                                                                >  1:677022/1‑140 (MQ=255)
                                                 ggCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAaca                                                                               <  2:556084/140‑1 (MQ=255)
                                                   cTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACt                                                                             <  1:1489573/140‑1 (MQ=255)
                                                    tGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGCGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGCGCGAACACtt                                                                            >  2:500567/1‑140 (MQ=255)
                                                      ccACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTATATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGaa                                                                                 <  1:1584384/133‑1 (MQ=255)
                                                      ccACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTATATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGaa                                                                                 >  2:1584384/1‑133 (MQ=255)
                                                           caGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTAt                                                                     <  1:124033/140‑1 (MQ=255)
                                                             gTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTc                                                                   <  2:677022/140‑1 (MQ=255)
                                                             gTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTc                                                                   <  1:1206026/140‑1 (MQ=255)
                                                                gcggcgCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTcgcg                                                                >  1:1358216/1‑140 (MQ=255)
                                                                 cggcgCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGt                                                               >  1:903756/1‑140 (MQ=255)
                                                                      ctgaaagctgaaaCCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGa                                                          >  1:792149/1‑140 (MQ=255)
                                                                           agctgaaaCCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGt                                                     <  2:711531/140‑1 (MQ=255)
                                                                           agctgaaaCCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGt                                                     <  1:1389557/140‑1 (MQ=255)
                                                                           agctgaaaCCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGt                                                     <  1:197959/140‑1 (MQ=255)
                                                                           agctgaaaCCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGt                                                     <  2:742667/140‑1 (MQ=255)
                                                                             ctgaaaCCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTc                                                   <  1:1010681/140‑1 (MQ=255)
                                                                                     aaaGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGAt                                           <  2:903756/140‑1 (MQ=255)
                                                                                             tACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTg                                   <  1:699562/140‑1 (MQ=255)
                                                                                             tACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTg                                   >  2:699562/1‑140 (MQ=255)
                                                                                               cTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGaa                                 <  1:1334641/140‑1 (MQ=255)
                                                                                               cTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGaa                                 <  2:849537/140‑1 (MQ=255)
                                                                                               cTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGaa                                 <  1:48779/140‑1 (MQ=255)
                                                                                                      cGGTGGCTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTa                          >  2:1434565/1‑140 (MQ=255)
                                                                                                            cTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGaa                                 >  2:312042/1‑127 (MQ=255)
                                                                                                            cTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGaa                                 <  1:312042/127‑1 (MQ=255)
                                                                                                            cTGTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCt                    >  2:1073222/1‑140 (MQ=255)
                                                                                                              gTATCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTc                  >  2:269120/1‑140 (MQ=255)
                                                                                                                 tCCCGCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCgaa               >  2:686245/1‑140 (MQ=255)
                                                                                                                     gCTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAAc           >  2:1702321/1‑140 (MQ=255)
                                                                                                                      cTGGTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAAct          >  1:466540/1‑140 (MQ=255)
                                                                                                                         gTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAActgct       >  2:811038/1‑140 (MQ=255)
                                                                                                                         gTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAActgct       >  1:1338222/1‑140 (MQ=255)
                                                                                                                         gTCACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAActgct       >  1:1031245/1‑140 (MQ=255)
                                                                                                                           cACTCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTgg     <  1:500567/140‑1 (MQ=255)
                                                                                                                              tCAGGCACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGa  >  2:1122685/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                      
CTCCTGCTTCGACGTCACCAAAGGTGATCTGATTGCTGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGA  >  NC_000913/3495285‑3495550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A