Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,658,278	G→A	intergenic (‑224/‑88)	arrS ← / → gadE	small regulatory RNA ArrS/DNA‑binding transcriptional activator GadE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,658,278	0	G	A	100.0%	428.9 / NA	118	intergenic (‑224/‑88)	arrS/gadE	small regulatory RNA ArrS/DNA‑binding transcriptional activator GadE
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (69/49); total (69/49)

Only 100 of 118 total aligned reads displayed.

TTGCTATTTACAAGCTGATAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCGTTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGG  >  NC_000913/3658145‑3658413

        tACAAGCTGATAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGaa                                                                                                                           <  1:505667/140‑1 (MQ=255)

          cAAGCTGATAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGaaca                                                                                                                         <  2:263178/140‑1 (MQ=255)

           aaGCTGATAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGaacaa                                                                                                                        <  1:1561305/140‑1 (MQ=255)

               tGATAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGAt                                                                                                                    <  2:175805/140‑1 (MQ=255)

               tGATAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGAt                                                                                                                    <  1:835696/140‑1 (MQ=255)

                gATAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGAtt                                                                                                                   >  1:339752/1‑140 (MQ=255)

                gATAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGAtt                                                                                                                   >  2:1003809/1‑140 (MQ=255)

                gATAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGAtt                                                                                                                   >  2:1081005/1‑140 (MQ=255)

                gATAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGAtt                                                                                                                   >  1:318639/1‑140 (MQ=255)

                gATAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGAtt                                                                                                                   >  1:1154439/1‑140 (MQ=255)

                gATAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGAtt                                                                                                                   >  1:1199264/1‑140 (MQ=255)

                gATAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGAtt                                                                                                                   <  2:630944/140‑1 (MQ=255)

                gATAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGAtt                                                                                                                   >  1:1590649/1‑140 (MQ=255)

                 aTAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGaac                                                                                                                          <  2:625611/132‑1 (MQ=255)

                 aTAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGaac                                                                                                                          >  1:625611/1‑132 (MQ=255)

                     caacCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGAc                                                                                                              <  1:993329/140‑1 (MQ=255)

                      aacCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACa                                                                                                             <  2:339752/140‑1 (MQ=255)

                       acCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACa                                                                                                             >  1:1682561/1‑139 (MQ=255)

                       acCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACa                                                                                                             <  2:1682561/139‑1 (MQ=255)

                        ccAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGAc                                                                                                              >  1:806953/1‑137 (MQ=255)

                        ccAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGAc                                                                                                              <  2:806953/137‑1 (MQ=255)

                           ggAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGAt                                                                                                        >  2:1115875/1‑140 (MQ=255)

                           ggAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGAt                                                                                                        <  1:331931/140‑1 (MQ=255)

                                cttacttaGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGt                                                                                                      >  2:1432779/1‑137 (MQ=255)

                                cttacttaGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGt                                                                                                      <  1:1432779/137‑1 (MQ=255)

                                       aGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGaa                                                                                            <  1:1003809/140‑1 (MQ=255)

                                        ggATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGaaa                                                                                           >  2:1567988/1‑140 (MQ=255)

                                         gATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGaaaa                                                                                          >  1:337784/1‑140 (MQ=255)

                                            cAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGa                                                                                       <  2:1460445/140‑1 (MQ=255)

                                              atatatGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATg                                                                                     >  1:964020/1‑140 (MQ=255)

                                              atatatGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATg                                                                                     >  2:1466986/1‑140 (MQ=255)

                                                   tGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACata                                                                                >  1:1450232/1‑140 (MQ=255)

                                                   tGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACata                                                                                >  2:600247/1‑140 (MQ=255)

                                                   tGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACata                                                                                >  2:1464803/1‑140 (MQ=255)

                                                       gTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGa                                                                                       >  1:983782/1‑129 (MQ=255)

                                                       gTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCg                                                                            >  1:501212/1‑140 (MQ=255)

                                                         gCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGaa                                                                          >  1:534201/1‑140 (MQ=255)

                                                         gCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGaa                                                                          >  2:4002/1‑140 (MQ=255)

                                                         gCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGaa                                                                          >  1:667392/1‑140 (MQ=255)

                                                         gCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGaa                                                                          >  1:95187/1‑140 (MQ=255)

                                                         gCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGaa                                                                          <  2:501212/140‑1 (MQ=255)

                                                         gCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGaa                                                                          >  2:224255/1‑140 (MQ=255)

                                                         gCGTGATGGATAAATCTGAAGTATAGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGaa                                                                          >  1:1152574/1‑140 (MQ=255)

                                                          cGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGa                                                                                       <  1:837564/126‑1 (MQ=255)

                                                          cGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGa                                                                                       >  2:837564/1‑126 (MQ=255)

                                                          cGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGaaa                                                                         >  1:378714/1‑140 (MQ=255)

                                                          cGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGaaa                                                                         <  1:600247/140‑1 (MQ=255)

                                                          cGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGaaa                                                                         >  2:929412/1‑140 (MQ=255)

                                                          cGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGaaa                                                                         >  1:835848/1‑140 (MQ=255)

                                                          cGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGaaa                                                                         >  1:1630897/1‑140 (MQ=255)

                                                            tGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACg                                                                       <  1:1115875/140‑1 (MQ=255)

                                                            tGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACg                                                                       >  2:113275/1‑140 (MQ=255)

                                                              aTGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGAt                                                                     <  1:906488/140‑1 (MQ=255)

                                                              aTGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGAt                                                                     <  2:964020/140‑1 (MQ=255)

                                                              aTGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGAt                                                                     <  2:95187/140‑1 (MQ=255)

                                                                ggATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATaa                                                                   <  2:30633/140‑1 (MQ=255)

                                                                ggATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATaa                                                                   <  1:113275/140‑1 (MQ=255)

                                                                  aTAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACg                                                                 >  2:850404/1‑140 (MQ=255)

                                                                     aaTCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCt                                                              <  2:1450232/140‑1 (MQ=255)

                                                                      aTCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATGTTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTa                                                             >  1:1483721/1‑140 (MQ=255)

                                                                         tGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAgg                                                          >  2:1163685/1‑140 (MQ=255)

                                                                           aaGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAg                                                        <  2:936434/140‑1 (MQ=255)

                                                                                 tGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGtt                                                  <  1:1308809/140‑1 (MQ=255)

                                                                                        tgtTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGAttt                                           >  1:113510/1‑140 (MQ=255)

                                                                                        tgtTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGAttt                                           >  1:519624/1‑140 (MQ=255)

                                                                                        tgtTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGAttt                                           >  2:832334/1‑140 (MQ=255)

                                                                                          ttAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGAttttt                                         >  2:755620/1‑140 (MQ=255)

                                                                                             aTAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTtctc                                      >  1:449508/1‑140 (MQ=255)

                                                                                              tAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCa                                     <  1:929412/140‑1 (MQ=255)

                                                                                                 aCGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGAttttt                                         <  2:1466473/133‑1 (MQ=255)

                                                                                                 aCGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGAttttt                                         >  1:1466473/1‑133 (MQ=255)

                                                                                                 aCGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGa                                  >  1:1387724/1‑140 (MQ=255)

                                                                                                  cGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATg                                   <  1:1367494/138‑1 (MQ=255)

                                                                                                  cGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATg                                   >  2:1367494/1‑138 (MQ=255)

                                                                                                  cGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGAc                                 >  1:678111/1‑140 (MQ=255)

                                                                                                  cGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGAc                                 >  1:912347/1‑140 (MQ=255)

                                                                                                  cGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGAc                                 >  1:697363/1‑140 (MQ=255)

                                                                                                  cGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGAc                                 >  1:892369/1‑140 (MQ=255)

                                                                                                    tATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGa                               >  1:144523/1‑140 (MQ=255)

                                                                                                    tATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGa                               >  2:1166322/1‑140 (MQ=255)

                                                                                                    tATTAGTTCACGAAGGGTAAAGTTCTTATAGGCATTTACCATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGa                               >  1:991697/1‑140 (MQ=255)

                                                                                                            cACGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGAttct                       >  2:369784/1‑140 (MQ=255)

                                                                                                              cGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGa                           <  1:698675/134‑1 (MQ=255)

                                                                                                              cGAAGGGTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGa                           >  2:698675/1‑134 (MQ=255)

                                                                                                                    gTAAAGTTCTTATATGCATTTACTAGATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTcttttctttt               >  2:1271135/1‑140 (MQ=255)

                                                                                                                    gTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTcttttctttt               >  1:186803/1‑140 (MQ=255)

                                                                                                                    gTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTcttttctttt               >  2:1311738/1‑140 (MQ=255)

                                                                                                                    gTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATG‑AAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGAttcttttc                   <  1:1457420/135‑1 (MQ=255)

                                                                                                                    gTAAAGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATG‑AAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGAttcttttc                   >  2:1457420/1‑135 (MQ=255)

                                                                                                                       aaGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACa            <  2:378714/140‑1 (MQ=255)

                                                                                                                       aaGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACa            >  1:1419569/1‑140 (MQ=255)

                                                                                                                       aaGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACa            <  1:4002/140‑1 (MQ=255)

                                                                                                                       aaGTTCTTATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACa            <  2:892369/140‑1 (MQ=255)

                                                                                                                             ttATAGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCtt      >  2:1471406/1‑140 (MQ=255)

                                                                                                                              tataGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCttt     >  1:623812/1‑140 (MQ=255)

                                                                                                                               ataGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCtttt    >  1:869592/1‑140 (MQ=255)

                                                                                                                                 aGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGTGCTTTTgg  <  1:224255/140‑1 (MQ=255)

                                                                                                                                 aGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTgg  <  2:144523/140‑1 (MQ=255)

                                                                                                                                 aGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTgg  <  2:1505329/140‑1 (MQ=255)

                                                                                                                                 aGGCATTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTgg  <  2:1590649/140‑1 (MQ=255)

TTGCTATTTACAAGCTGATAACAACCAGGAATCTTACTTAGGATCAATATATGGAGTGCGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGTAAAGTTCTTATAGGCGTTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATATTCGAAACGATAACGGCTAAGGAGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGG  >  NC_000913/3658145‑3658413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A