Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 183 98.6 3427438 96.9% 3321187 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,012,504 T→C T105A (ACA→GCA)  metR ← DNA‑binding transcriptional dual regulator MetR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,012,5040TC98.3% 417.5 / ‑5.6 115T105A (ACA→GCA) metRDNA‑binding transcriptional dual regulator MetR
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (58/55);  total (60/55)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

CGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGTCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGT  >  NC_000913/4012368‑4012640
                                                                                                                                        |                                                                                                                                        
cgcgGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAg                                                                                                                                       >  1:133623/1‑140 (MQ=255)
   ggCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTgccagcca                                                                                                                                    <  1:1411059/140‑1 (MQ=255)
     cAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACt                                                                                                                                  <  1:1567515/140‑1 (MQ=255)
        aaTATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGaa                                                                                                                               <  1:312711/140‑1 (MQ=255)
             cGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAg                                                                                                                          <  1:1540856/140‑1 (MQ=255)
               gACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCt                                                                                                                        >  1:969463/1‑140 (MQ=255)
               gACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTACCAGCCACTGAATACAGCt                                                                                                                        >  1:1013427/1‑140 (MQ=255)
                  gTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAg                                                                                                                                       <  2:47738/122‑1 (MQ=255)
                  gTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATg                                                                                                                     >  1:233187/1‑140 (MQ=255)
                   tCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATa                                                                                                                             >  1:382785/1‑131 (MQ=255)
                   tCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATa                                                                                                                             <  2:382785/131‑1 (MQ=255)
                   tCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATgg                                                                                                                    <  1:111755/140‑1 (MQ=255)
                    cATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGaa                                                                                                                               >  2:1094229/1‑128 (MQ=255)
                    cATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGaa                                                                                                                               <  1:1094229/128‑1 (MQ=255)
                    cATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGc                                                                                                                   >  2:144171/1‑140 (MQ=255)
                    cATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGc                                                                                                                   >  2:826555/1‑140 (MQ=255)
                     aTTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCa                                                                                                                  <  2:590439/140‑1 (MQ=255)
                          cAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCaa                                                                                                             <  2:233187/140‑1 (MQ=255)
                          cAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCaa                                                                                                             <  1:233128/140‑1 (MQ=255)
                            gATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATgg                                                                                                                    <  1:1290753/131‑1 (MQ=255)
                            gATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATgg                                                                                                                    >  2:1290753/1‑131 (MQ=255)
                            gATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATg                                                                                                           >  2:158081/1‑140 (MQ=255)
                             aTCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATgg                                                                                                          >  1:134831/1‑140 (MQ=255)
                              tCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGc                                                                                                         >  2:231776/1‑140 (MQ=255)
                               ccAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCa                                                                                                        <  1:308552/140‑1 (MQ=255)
                                 aGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAAt                                                                                                      >  2:55983/1‑140 (MQ=255)
                                   ctctCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATgc                                                                                                    >  1:1499854/1‑140 (MQ=255)
                                   ctctCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATgc                                                                                                    >  1:434984/1‑140 (MQ=255)
                                      tcCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCa                                                                                                 <  2:51451/140‑1 (MQ=255)
                                      tcCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCa                                                                                                 >  1:794442/1‑140 (MQ=255)
                                        ccTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGa                                                                                               >  2:389803/1‑140 (MQ=255)
                                        ccTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGa                                                                                               >  1:61142/1‑140 (MQ=255)
                                          tGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGAcg                                                                                             >  1:704832/1‑140 (MQ=255)
                                           gTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGAcgc                                                                                            >  1:339886/1‑140 (MQ=255)
                                            ttGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGAcgcg                                                                                           <  1:158081/140‑1 (MQ=255)
                                             tGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGa                                                                                               <  1:68795/135‑1 (MQ=255)
                                             tGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGa                                                                                               >  2:68795/1‑135 (MQ=255)
                                             tGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGt                                                                                          >  1:429776/1‑140 (MQ=255)
                                             tGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGt                                                                                          <  2:61142/140‑1 (MQ=255)
                                                  ggCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATgcg                                                                                                   >  2:1127275/1‑126 (MQ=255)
                                                  ggCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATgcg                                                                                                   <  1:1127275/126‑1 (MQ=255)
                                                   gCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTctgctg                                                                                    <  1:144171/140‑1 (MQ=255)
                                                   gCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTctgctg                                                                                    <  2:339886/140‑1 (MQ=255)
                                                   gCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTctgctg                                                                                    <  1:231776/140‑1 (MQ=255)
                                                    cGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCtgctgc                                                                                   >  1:1247400/1‑140 (MQ=255)
                                                      ggCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCgg                                                                                 >  1:664429/1‑140 (MQ=255)
                                                      ggCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCgg                                                                                 >  2:356827/1‑140 (MQ=255)
                                                        cTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGtt                                                                               <  2:478982/140‑1 (MQ=255)
                                                             ggTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTg                                                                          <  2:794442/140‑1 (MQ=255)
                                                             ggTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTg                                                                          <  1:389803/140‑1 (MQ=255)
                                                                cAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAg                                                                       >  2:332118/1‑140 (MQ=255)
                                                                cAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATCGCAg                                                                       <  2:914143/140‑1 (MQ=255)
                                                                 aaaTGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAgg                                                                      >  2:1678098/1‑140 (MQ=255)
                                                                 aaaTGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAgg                                                                      <  2:664429/140‑1 (MQ=255)
                                                                 aaaTGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAgg                                                                      <  2:13484/140‑1 (MQ=255)
                                                                        aCGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAg                                                               >  1:201383/1‑140 (MQ=255)
                                                                          gCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAggg                                                             <  1:55983/140‑1 (MQ=255)
                                                                                       tCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTg                                                <  2:434984/140‑1 (MQ=255)
                                                                                         cATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCg                                              <  2:1247400/140‑1 (MQ=255)
                                                                                          aTCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCgg                                             >  2:1275344/1‑140 (MQ=255)
                                                                                             tctACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAg                                          >  2:615509/1‑140 (MQ=255)
                                                                                                 ccTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTaa                                                    <  2:474964/126‑1 (MQ=255)
                                                                                                 ccTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTaa                                                    >  1:474964/1‑126 (MQ=255)
                                                                                                    gCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGACAGTACCTgg                                   >  1:1095180/1‑140 (MQ=255)
                                                                                                     cGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGt                                  >  1:83618/1‑140 (MQ=255)
                                                                                                     cGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGt                                  >  2:298178/1‑140 (MQ=255)
                                                                                                       gCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGttt                                >  2:1668012/1‑140 (MQ=255)
                                                                                                       gCCAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGttt                                >  2:598831/1‑140 (MQ=255)
                                                                                                        ccAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTg                               <  2:558660/140‑1 (MQ=255)
                                                                                                         cAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCgg                                             >  1:1470711/1‑125 (MQ=255)
                                                                                                         cAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCgg                                             <  2:1470711/125‑1 (MQ=255)
                                                                                                         cAGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGc                              >  2:1291745/1‑140 (MQ=255)
                                                                                                          aGTTCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGcc                             >  2:1025820/1‑140 (MQ=255)
                                                                                                            ttCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAg                           >  2:1055753/1‑140 (MQ=255)
                                                                                                             tCTTATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGt                          <  1:1353557/140‑1 (MQ=255)
                                                                                                              cTTATGGAAATTTTCTAACGCGGGTGCCAGCAACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGtt                         <  2:721724/140‑1 (MQ=255)
                                                                                                                tATGGAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGc                       <  1:826555/140‑1 (MQ=255)
                                                                                                                    gAAATTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACa                   <  1:1238638/140‑1 (MQ=255)
                                                                                                                      aaTTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAgg                 >  2:313114/1‑140 (MQ=255)
                                                                                                                      aaTTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAgg                 >  2:462479/1‑140 (MQ=255)
                                                                                                                       aTTTTCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGa                >  1:1278338/1‑140 (MQ=255)
                                                                                                                         tttCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGAtt              >  1:935896/1‑140 (MQ=255)
                                                                                                                         tttCTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGAtt              >  2:731696/1‑140 (MQ=255)
                                                                                                                            cTAACGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTtct           <  1:332118/140‑1 (MQ=255)
                                                                                                                              aaCGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTcc         <  2:1334030/140‑1 (MQ=255)
                                                                                                                              aaCGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTcc         <  2:83618/140‑1 (MQ=255)
                                                                                                                              aaCGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTcc         <  1:1055753/140‑1 (MQ=255)
                                                                                                                              aaCGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTcc         <  1:1291745/140‑1 (MQ=255)
                                                                                                                              aaCGCGGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTcc         <  1:598831/140‑1 (MQ=255)
                                                                                                                                cgcgGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCt       <  2:1499854/140‑1 (MQ=255)
                                                                                                                                  cgGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGc     <  1:1025820/140‑1 (MQ=255)
                                                                                                                                  cgGGTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGc     <  1:356827/140‑1 (MQ=255)
                                                                                                                                   gggTGCCAGCCACTGAATACGGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCg    <  1:1369800/140‑1 (MQ=255)
                                                                                                                                   gggTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCg    <  1:27764/140‑1 (MQ=255)
                                                                                                                                   gggTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCg    <  1:251837/140‑1 (MQ=255)
                                                                                                                                    ggTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGa                <  1:835192/127‑1 (MQ=255)
                                                                                                                                    ggTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGa                >  2:835192/1‑127 (MQ=255)
                                                                                                                                    ggTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCgg   >  1:910517/1‑140 (MQ=255)
                                                                                                                                    ggTGCCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCgg   >  2:1239585/1‑140 (MQ=255)
                                                                                                                                     gtgcCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCTAGTTGCAACAGGATTTCTCCCTGCGgt  >  2:1338975/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
CGCGGCAGAATATCGGACGTCATTACCAGATCCAGCTCTCCCTGTTGCAAGGCGGGCTGCGGGTCAAATGTCACGCCCGATTTAAAATCCATCTCTACCTGCGGCCAGTTCTTATGGAAATTTTCTAACGCGGGTGTCAGCCACTGAATACAGCTATGGCACTCAATGGCAATGCGCAGACGCGTCTGCTGCGGTTCATTGCAGGCTTGCAGGGCCTGGCTAATTTGCGGCAGTACCTGGTTTGCCAGTTGCAACAGGATTTCTCCCTGCGGT  >  NC_000913/4012368‑4012640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A