Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,359,603	Δ1 bp	coding (429/1335 nt)	cadB ←	lysine:cadaverine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,359,602	0	T	.	100.0%	560.1 / NA	131	coding (430/1335 nt)	cadB	lysine:cadaverine antiporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (55/76); total (55/76)

Only 100 of 138 total aligned reads displayed.

ATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGCTTACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAAAGATAAGATACAGCGGTAATACCAATCGC  >  NC_000913/4359463‑4359737

    tCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACAGCtaccc                                                                                                                                     >  1:432263/1‑136 (MQ=255)

     cGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGTCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCa                                                                                                                                   >  2:1003652/1‑140 (MQ=255)

     cGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCa                                                                                                                                   >  2:660897/1‑140 (MQ=255)

     cGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCa                                                                                                                                   >  2:1424778/1‑140 (MQ=255)

     cGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCa                                                                                                                                   >  1:173434/1‑140 (MQ=255)

       cATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAg                                                                                                                                 >  2:1168889/1‑140 (MQ=255)

           aCCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTAcc                                                                                                                             <  2:389148/140‑1 (MQ=255)

               tCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTAccgccg                                                                                                                         >  2:510820/1‑140 (MQ=255)

               tCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACAAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTAccgccg                                                                                                                         <  2:1549691/140‑1 (MQ=255)

               tCAGTGGTATCCGCAGCATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTAccgccg                                                                                                                         <  2:1536038/140‑1 (MQ=255)

                cAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGa                                                                                                                        >  2:498500/1‑140 (MQ=255)

                 aGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAg                                                                                                                       <  2:615046/140‑1 (MQ=255)

                 aGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAg                                                                                                                       <  1:660897/140‑1 (MQ=255)

                    ggTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAg                                                                                                                       <  1:429523/137‑1 (MQ=255)

                    ggTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCat                                                                                                                    <  1:615854/140‑1 (MQ=255)

                      tATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCatat                                                                                                                  >  2:105406/1‑140 (MQ=255)

                      tATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCatat                                                                                                                  >  1:1704588/1‑140 (MQ=255)

                      tATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCatat                                                                                                                  >  1:116172/1‑140 (MQ=255)

                      tATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCatat                                                                                                                  >  1:1108908/1‑140 (MQ=255)

                         ccGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTa                                                                                                               <  2:1699058/140‑1 (MQ=255)

                          cGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTAc                                                                                                              >  2:729008/1‑140 (MQ=255)

                           gCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAg                                                                                                                       >  2:1045898/1‑130 (MQ=255)

                           gCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAg                                                                                                                       <  1:1045898/130‑1 (MQ=255)

                           gCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACa                                                                                                             <  1:1501926/140‑1 (MQ=255)

                           gCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACa                                                                                                             <  1:435683/140‑1 (MQ=255)

                           gCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACa                                                                                                             <  1:688377/140‑1 (MQ=255)

                           gCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACa                                                                                                             <  1:1187317/140‑1 (MQ=255)

                            cAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACNNNNGNACCGCCGAGCATATTTACaa                                                                                                            <  2:1349549/140‑1 (MQ=255)

                            cAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACaa                                                                                                            >  2:334614/1‑140 (MQ=255)

                             aGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACaaa                                                                                                           <  2:1360459/140‑1 (MQ=255)

                             aGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACaaa                                                                                                           >  1:1133643/1‑140 (MQ=255)

                             aGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACaaa                                                                                                           >  2:1636613/1‑140 (MQ=255)

                                aTTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGt                                                                                                        <  1:218615/140‑1 (MQ=255)

                                 ttCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTa                                                                                                       >  1:1022856/1‑140 (MQ=255)

                                  tCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTaa                                                                                                      >  2:1479716/1‑140 (MQ=255)

                                       ttAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATAcc                                                                                                 <  2:1477186/140‑1 (MQ=255)

                                         aGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCa                                                                                               <  2:1108908/140‑1 (MQ=255)

                                         aGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCa                                                                                               <  2:1117933/140‑1 (MQ=255)

                                         aGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCa                                                                                               >  2:889712/1‑140 (MQ=255)

                                           cTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGa                                                                                             <  2:1267528/140‑1 (MQ=255)

                                            tGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAGTAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGAc                                                                                            <  1:975728/140‑1 (MQ=255)

                                            tGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGAc                                                                                            <  2:28469/140‑1 (MQ=255)

                                            tGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGAc                                                                                            <  1:1424778/140‑1 (MQ=255)

                                              cATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAAT‑AGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGAt                                                                                         >  1:199110/1‑140 (MQ=255)

                                               aTAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGAt                                                                                         <  1:889712/140‑1 (MQ=255)

                                               aTAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGAt                                                                                         <  1:64517/140‑1 (MQ=255)

                                               aTAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGAt                                                                                         <  1:988777/140‑1 (MQ=255)

                                                      gCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATa                                                                                  <  2:565839/140‑1 (MQ=255)

                                                          cATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAgg                                                                              <  1:551973/140‑1 (MQ=255)

                                                               aaCCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATa                                                                         <  1:717086/140‑1 (MQ=255)

                                                                 ccAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATAcc                                                                       <  1:1231003/140‑1 (MQ=255)

                                                                    aTGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCCCCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACccgc                                                                    <  2:1105992/140‑1 (MQ=255)

                                                                    aTGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATCCccgc                                                                    <  2:1402821/140‑1 (MQ=255)

                                                                     tGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACccgcc                                                                   >  1:251455/1‑140 (MQ=255)

                                                                         agccaACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGaa                                                               <  1:334614/140‑1 (MQ=255)

                                                                          gccaACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAAc                                                              >  1:575291/1‑140 (MQ=255)

                                                                            caacaaTAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAg                                                            <  1:1003652/140‑1 (MQ=255)

                                                                            caacaaTAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAg                                                            <  1:1541551/140‑1 (MQ=255)

                                                                            caacaaTAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAg                                                            <  1:37924/140‑1 (MQ=255)

                                                                             aacaaAAGAATTCACCCCCACA‑GAAAAAGAACCAGCCCCAGACCAATAGTGGTTAAACGGC‑TCCCCAAGTCCCGCCGAGCATATTTACAAGGGTAAATCCCCAGACGATAGCAATACAGACGATACCCGCCGGAACAGGa                                                          <  2:199110/140‑1 (MQ=255)

                                                                              acaaTAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGa                                                          <  1:498500/140‑1 (MQ=255)

                                                                              acaaTAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGa                                                          <  2:747676/140‑1 (MQ=255)

                                                                              acaaTAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGa                                                          <  1:729008/140‑1 (MQ=255)

                                                                                   aGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCAtt                                                     <  1:105406/140‑1 (MQ=255)

                                                                                      aGTCATCACCACAGGAATAAGAACCAGCACCAGACAAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTaa                                                  <  1:1472473/140‑1 (MQ=255)

                                                                                       gTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAAt                                                 >  2:191819/1‑140 (MQ=255)

                                                                                        tcatcaCCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATCCCCGCCGGAACAGGa                                                          <  2:764291/130‑1 (MQ=255)

                                                                                        tcatcaCCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGa                                                          >  1:764291/1‑130 (MQ=255)

                                                                                         catcaCCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATAc                                               >  2:67843/1‑140 (MQ=255)

                                                                                         catcaCCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATAc                                               <  2:888945/140‑1 (MQ=255)

                                                                                         catcaCCACAGGAATAAAACCCACCACCAGACCAAAAGTGGTAAAAC‑GCCTACCCAAGTCCCCCCGACCATATTTACAAAGGAAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATAc                                               <  2:405316/140‑1 (MQ=255)

                                                                                          attaccacAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGa                                                          <  2:502751/125‑1 (MQ=255)

                                                                                          attaccacAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGa                                                          >  1:502751/4‑128 (MQ=255)

                                                                                            caccacAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTgg                                            >  2:613765/1‑140 (MQ=255)

                                                                                             cccacAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TCCCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTggg                                           <  1:907758/139‑1 (MQ=255)

                                                                                                acaGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGgaag                                        <  2:198131/140‑1 (MQ=255)

                                                                                                 caGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGgaaga                                       >  1:1084275/1‑140 (MQ=255)

                                                                                                    gAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAgg                                    >  2:322918/1‑140 (MQ=255)

                                                                                                      aTAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAgg                                    >  1:124857/1‑138 (MQ=255)

                                                                                                       tAAGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTgg                                 <  1:832219/140‑1 (MQ=255)

                                                                                                        aaGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGa                                <  1:510820/140‑1 (MQ=255)

                                                                                                        aaGAACCAGCACCAGACAAATAGTGGTTAAACGGC‑TCCCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGa                                <  2:1255297/140‑1 (MQ=255)

                                                                                                         aGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTCCCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGaa                               <  1:939536/140‑1 (MQ=255)

                                                                                                         aGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATCCCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGaa                               <  2:1704588/140‑1 (MQ=255)

                                                                                                         aGAACCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGaa                               <  2:116172/140‑1 (MQ=255)

                                                                                                           acccAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTCCCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAaag                             <  1:142864/138‑1 (MQ=255)

                                                                                                            aCCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAaaga                            >  1:242501/1‑140 (MQ=255)

                                                                                                            aCCAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAaaga                            <  1:613765/140‑1 (MQ=255)

                                                                                                              cAGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAaagata                          >  2:1189937/1‑140 (MQ=255)

                                                                                                               aGCCCCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAaagataa                         <  1:86283/140‑1 (MQ=255)

                                                                                                               aGCACCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAaagataa                         >  2:1588957/1‑140 (MQ=255)

                                                                                                                  aCCAGACCAATAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAaagataagat                      >  1:1694000/1‑140 (MQ=255)

                                                                                                                           aTAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAAAGATAAGATACAGCGGTa             >  1:1590464/1‑140 (MQ=255)

                                                                                                                           aTAGTGGTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAAAGATAAGATACAGCGGTa             <  2:1426265/140‑1 (MQ=255)

                                                                                                                                 gTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAAAGATAAGATACAGCGGTAATACCa       >  1:109121/1‑140 (MQ=255)

                                                                                                                                 gTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAAAGATAAGATACAGCGGTAATACCa       >  1:395448/1‑140 (MQ=255)

                                                                                                                                 gTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAAAGATAAGATACAGCGGTAATACCa       >  2:1075871/1‑140 (MQ=255)

                                                                                                                                 gTTAAACGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAAAGATAAGATACAGCGGTAATACCa       >  2:1036525/1‑140 (MQ=255)

                                                                                                                                    aaaCGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAAAGATAAGATACAGCGGTAATACCAATc    <  1:1168889/140‑1 (MQ=255)

                                                                                                                                      aCGGC‑TACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAAAGATAAGATACAGCGGTAATACCAATCGc  <  1:67843/140‑1 (MQ=255)

ATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACCACAGGAATAAGAACCAGCACCAGACCAATAGTGGTTAAACGGCTTACCCAAGTACCGCCGAGCATATTTACAAAGGTAAATACCCAGACGATAGCAATACAGGCGATACCCGCCGGAACAGGATCATTTAATACTGGGAAGAAGGTGGAAAGATAAGATACAGCGGTAATACCAATCGC  >  NC_000913/4359463‑4359737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A