Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 183 98.6 3427438 96.9% 3321187 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,429,216 (A)6→7 coding (282/621 nt) fklB → FKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,2101.A97.9% 329.2 / ‑1.0 93coding (276/621 nt)fklBFKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base A (45/46);  total (46/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 102 total aligned reads displayed.

GGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑C‑AAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGT  >  NC_000913/4429072‑4429343
                                                                                                                                            |                                                                                                                                     
ggAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑C‑a                                                                                                                                      >  1:1585284/1‑140 (MQ=255)
    ggCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑C‑aaaaa                                                                                                                                  >  1:481519/1‑140 (MQ=255)
      cAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑C‑aaaaaaa                                                                                                                                >  2:883864/1‑139 (MQ=255)
       aaaCATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑C‑aaaaaaag                                                                                                                               <  2:340719/140‑3 (MQ=255)
       aaaCATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑C‑aaaaaaag                                                                                                                               <  1:700289/140‑3 (MQ=255)
       aaaCATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑C‑aaaaaaag                                                                                                                               <  1:540515/140‑3 (MQ=255)
       aaaCATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑C‑aaaaaaag                                                                                                                               <  1:406803/140‑3 (MQ=255)
       aaaCATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑C‑aaaaaaag                                                                                                                               >  1:333577/1‑138 (MQ=255)
           aTCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAgg                                                                                                                            >  1:327979/1‑140 (MQ=255)
            tCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGgt                                                                                                                           <  1:360370/140‑1 (MQ=255)
              cGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGgtgt                                                                                                                         <  2:773924/140‑1 (MQ=255)
               ggCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGtgtg                                                                                                                        >  1:1496327/1‑140 (MQ=255)
                 cTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGaa                                                                                                                      <  1:799075/140‑1 (MQ=255)
                  tGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAAt                                                                                                                     <  2:217034/140‑1 (MQ=255)
                   gTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATa                                                                                                                    <  1:131911/140‑1 (MQ=255)
                     tCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGgt                                                                                                                           <  2:573643/131‑1 (MQ=255)
                     tCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGgt                                                                                                                           >  1:573643/1‑131 (MQ=255)
                       cGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCAc                                                                                                                <  2:254692/140‑1 (MQ=255)
                             aTGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATc                                                                                                          <  1:1481074/140‑1 (MQ=255)
                              tgtgGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCt                                                                                                         >  2:36132/1‑140 (MQ=255)
                               gtggtgCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCTCCGAATCTg                                                                                                        <  2:1407490/140‑1 (MQ=255)
                               gtggtgCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTg                                                                                                        <  2:1407479/140‑1 (MQ=255)
                                   tgCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCt                                                                                                    >  2:248940/1‑140 (MQ=255)
                                       tCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCaa                                                                                                >  2:1324869/1‑140 (MQ=255)
                                        cgcgcgCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAAt                                                                                               >  1:291413/1‑140 (MQ=255)
                                          cgcgcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTc                                                                                             >  2:846194/1‑140 (MQ=255)
                                            cgcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCcg                                                                                           <  2:190009/140‑1 (MQ=255)
                                             gcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCcgc                                                                                          >  1:1711780/1‑140 (MQ=255)
                                             gcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCcgc                                                                                          >  2:160144/1‑140 (MQ=255)
                                                gCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTg                                                                                       <  2:481519/140‑1 (MQ=255)
                                                 cGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGa                                                                                      <  1:1437239/140‑1 (MQ=255)
                                                  gTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                                                                                     <  1:160144/140‑1 (MQ=255)
                                                  gTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                                                                                     <  2:333577/140‑1 (MQ=255)
                                                     aaaTCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCaa                                                                                  >  1:1270593/1‑140 (MQ=255)
                                                      aaTCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAAc                                                                                 <  2:291413/140‑1 (MQ=255)
                                                      aaTCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAAc                                                                                 <  2:1711780/140‑1 (MQ=255)
                                                         ccACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAg                                                                              >  2:1493879/1‑140 (MQ=255)
                                                         ccACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAg                                                                              >  1:1432448/1‑140 (MQ=255)
                                                             gAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTg                                                                          >  2:1343026/1‑140 (MQ=255)
                                                                  cgcCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTCGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGgc                                                                     >  2:51745/1‑140 (MQ=255)
                                                                   gcCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGgcg                                                                    <  2:1585284/140‑1 (MQ=255)
                                                                   gcCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGgcg                                                                    <  1:1554279/140‑1 (MQ=255)
                                                                        tGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAAtt                                                               <  2:1219518/140‑1 (MQ=255)
                                                                        tGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAAtt                                                               <  1:846194/140‑1 (MQ=255)
                                                                         gCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTc                                                              <  2:1270593/140‑1 (MQ=255)
                                                                          ccGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTcc                                                             <  1:36132/140‑1 (MQ=255)
                                                                             ttCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCgg                                                           <  2:663452/139‑1 (MQ=255)
                                                                             ttCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCgg                                                           >  1:663452/1‑139 (MQ=255)
                                                                             ttCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGc                                                          >  1:582179/1‑140 (MQ=255)
                                                                                  cgtcAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTa                                                     >  1:1207689/1‑140 (MQ=255)
                                                                                    tcAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTacc                                                   >  1:414376/1‑140 (MQ=255)
                                                                                       gCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAAtt                                                               >  1:1501434/1‑125 (MQ=255)
                                                                                       gCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAAtt                                                               <  2:1501434/125‑1 (MQ=255)
                                                                                        cGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTaccgacc                                               >  2:1450702/1‑140 (MQ=255)
                                                                                         gTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTaccgaccg                                              <  2:327979/140‑1 (MQ=255)
                                                                                            tCCAGGCGATGGCTGGTGAAGGTGTGAAATCCCTGGAAGAAAGCGCTA‑AAAAAAGAAGGCGTGAATATCACCGAAGCTGGCCTGCAAGTCCGCGTGATCAACCAGGGTTAGGGCGCAATCCCGGCACGTACCGACCGCGt                                           <  2:383392/140‑1 (MQ=255)
                                                                                            tCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGt                                           <  2:431928/140‑1 (MQ=255)
                                                                                                ggCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCgt                                       <  1:705525/140‑1 (MQ=255)
                                                                                                  cGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCgtgt                                     >  2:1404454/1‑140 (MQ=255)
                                                                                                   gATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGtt                                    <  2:1432448/140‑1 (MQ=255)
                                                                                                     tGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCgt                                       >  2:1267308/1‑135 (MQ=255)
                                                                                                     tGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCgt                                       <  1:1267308/135‑1 (MQ=255)
                                                                                                     tGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCa                                  >  2:1476325/1‑140 (MQ=255)
                                                                                                     tGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCa                                  >  2:226057/1‑140 (MQ=255)
                                                                                                     tGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCa                                  >  2:508583/1‑140 (MQ=255)
                                                                                                     tGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGGGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCa                                  >  1:1236986/1‑140 (MQ=255)
                                                                                                      ggCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTc                                         <  1:378145/132‑1 (MQ=255)
                                                                                                      ggCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTc                                         >  2:378145/1‑132 (MQ=255)
                                                                                                      ggCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTc                                         >  2:602984/1‑132 (MQ=255)
                                                                                                      ggCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTc                                         <  1:602984/132‑1 (MQ=255)
                                                                                                      ggCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCgt                                       <  1:28988/134‑1 (MQ=255)
                                                                                                      ggCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCgt                                       >  2:28988/1‑134 (MQ=255)
                                                                                                      ggCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCAt                                 >  2:1230425/1‑140 (MQ=255)
                                                                                                      ggCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCAt                                 >  2:257301/1‑140 (MQ=255)
                                                                                                        ctgctgAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTa                               <  2:414376/140‑1 (MQ=255)
                                                                                                        ctgctgAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTa                               <  2:978846/140‑1 (MQ=255)
                                                                                                        ctgctgAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTa                               <  2:110871/140‑1 (MQ=255)
                                                                                                        ctgctgAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTa                               <  1:508583/140‑1 (MQ=255)
                                                                                                        ctgctgAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTa                               <  1:1476325/140‑1 (MQ=255)
                                                                                                          gctgAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTaca                             <  1:1324869/140‑1 (MQ=255)
                                                                                                           ctgAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTacac                            >  1:1022750/1‑140 (MQ=255)
                                                                                                           ctgAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTa                               >  2:216177/1‑137 (MQ=255)
                                                                                                           ctgAAGGTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTa                               <  1:216177/137‑1 (MQ=255)
                                                                                                           ctgAAGGTGTGAAATACCTGGAAGAAAACGC‑C‑AAAAAAAGAAGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCa                                  >  1:108038/1‑133 (MQ=255)
                                                                                                           ctgAAGGTGTGAAATACCTGGAAGAAAACGC‑C‑AAAAAAAGAAGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCa                                  <  2:108038/133‑1 (MQ=255)
                                                                                                                ggTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTa                       >  2:753139/1‑140 (MQ=255)
                                                                                                                ggTGTGAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTa                       <  1:1450702/140‑1 (MQ=255)
                                                                                                                    tgAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACt                   >  2:1700534/1‑140 (MQ=255)
                                                                                                                    tgAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACt                   >  1:72868/1‑140 (MQ=255)
                                                                                                                     gAAATACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTg                  >  1:1025022/1‑140 (MQ=255)
                                                                                                                      aaaTACCTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGa                 >  2:1256203/1‑140 (MQ=255)
                                                                                                                           ccTGGAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGAc            >  2:55945/1‑140 (MQ=255)
                                                                                                                              ggAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGc         >  2:38807/1‑140 (MQ=255)
                                                                                                                              ggAAGAAAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGc         >  2:1136699/1‑140 (MQ=255)
                                                                                                                               gaagaaAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCa        <  1:1136699/140‑1 (MQ=255)
                                                                                                                               gaagaaAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATAGACGGCa        <  1:1230425/140‑1 (MQ=255)
                                                                                                                                 agaaAACGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCAcc      >  1:1458861/1‑140 (MQ=255)
                                                                                                                                    aaaCGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCgtg   <  2:503558/140‑1 (MQ=255)
                                                                                                                                     aaCGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCgtgt  <  1:1256203/140‑1 (MQ=255)
                                                                                                                                     aaCGC‑CAAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCgtgt  >  1:840938/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                     
GGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGC‑C‑AAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGT  >  NC_000913/4429072‑4429343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A