Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,567,801	A→G	V240A (GTG→GCG)	mdtM ←	multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,567,801	0	A	G	100.0%	313.4 / NA	83	V240A (GTG→GCG)	mdtM	multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (37/46); total (37/46)

GCCAGATAAACCGCGGTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCACCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGGCGCTAAACGGAACCGCGC  >  NC_000913/4567664‑4567936

gCCAGATAAACCGCGGTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGcc                                                                                                                                       >  1:1211947/1‑140 (MQ=255)

gCCAGATAAACCGCGGTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGcc                                                                                                                                       >  1:1503009/1‑140 (MQ=255)

   aGATAAACCGCGGTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGc                                                                                                                                    <  2:466595/140‑1 (MQ=255)

     aTAAACCGCGGTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCga                                                                                                                                  <  1:1184419/140‑1 (MQ=255)

          ccGCGGTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAg                                                                                                                             <  2:111473/140‑1 (MQ=255)

          ccGCGGTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAg                                                                                                                             <  1:596062/140‑1 (MQ=255)

          ccGCGGTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAg                                                                                                                             <  1:818217/140‑1 (MQ=255)

            gcgGTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGcc                                                                                                                           >  1:552966/1‑140 (MQ=255)

             cgGTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCa                                                                                                                          <  2:511208/140‑1 (MQ=255)

             cgGTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCa                                                                                                                          <  2:1104335/140‑1 (MQ=255)

              ggTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCAc                                                                                                                         >  2:279349/1‑140 (MQ=255)

              ggTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCAc                                                                                                                         >  2:1268255/1‑140 (MQ=255)

               gTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCAcc                                                                                                                        <  2:510157/140‑1 (MQ=255)

               gTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCAcc                                                                                                                        <  2:1107972/140‑1 (MQ=255)

               gTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCAcc                                                                                                                        >  2:811103/1‑140 (MQ=255)

               gTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCAcc                                                                                                                        <  2:519144/140‑1 (MQ=255)

               gTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCAcc                                                                                                                        >  1:1570608/1‑140 (MQ=255)

                   ggtcggATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGc                                                                                                                    <  1:1514326/140‑1 (MQ=255)

                    gtcggATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCt                                                                                                                   >  1:934729/1‑140 (MQ=255)

                       ggATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCAcc                                                                                                                        <  2:805698/132‑1 (MQ=255)

                       ggATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCAcc                                                                                                                        >  1:805698/1‑132 (MQ=255)

                       ggATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCtcat                                                                                                                <  1:2412/140‑1 (MQ=255)

                       ggATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCtcat                                                                                                                <  1:641971/140‑1 (MQ=255)

                         aTCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAg                                                                                                                             <  1:1481694/125‑1 (MQ=255)

                         aTCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAg                                                                                                                             >  2:1481694/1‑125 (MQ=255)

                          tCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGTGACAGCCACCCAGCTcatcat                                                                                                             <  2:1211947/140‑1 (MQ=255)

                          tCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTcatcat                                                                                                             <  1:811103/140‑1 (MQ=255)

                          tCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTcatcat                                                                                                             <  2:307260/140‑1 (MQ=255)

                          tCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTcatcat                                                                                                             <  2:1575644/140‑1 (MQ=255)

                            tttAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCTAGCTCATCATCg                                                                                                           >  2:916983/1‑140 (MQ=255)

                                aCAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCTAGCTCATCATCGGGat                                                                                                       <  1:916983/140‑1 (MQ=255)

                                aCAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGat                                                                                                       <  1:1071148/140‑1 (MQ=255)

                                aCAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGat                                                                                                       <  2:1570608/140‑1 (MQ=255)

                                 cAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGata                                                                                                      <  1:1322030/140‑1 (MQ=255)

                                 cAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGata                                                                                                      <  2:641854/140‑1 (MQ=255)

                                   aaaCGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGAtata                                                                                                    >  1:923042/1‑140 (MQ=255)

                                    aaCGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAg                                                                                                   >  2:344292/1‑140 (MQ=255)

                                     aCGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTcatcat                                                                                                             >  1:1264744/1‑129 (MQ=255)

                                     aCGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTcatcat                                                                                                             <  2:1264744/129‑1 (MQ=255)

                                     aCGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGc                                                                                                  <  1:632629/140‑1 (MQ=255)

                                     aCGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGc                                                                                                  <  2:1146793/140‑1 (MQ=255)

                                      cgcgCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCt                                                                                                 >  2:591988/1‑140 (MQ=255)

                                      cgcgCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCt                                                                                                 >  2:1018591/1‑140 (MQ=255)

                                      cgcgCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGCTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCt                                                                                                 >  2:1486396/1‑140 (MQ=255)

                                           cACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAag                                                                                            <  1:498542/140‑1 (MQ=255)

                                               aTGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGAt                                                                                        <  2:1687587/140‑1 (MQ=255)

                                                  gCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGt                                                                                     <  2:923042/140‑1 (MQ=255)

                                                    aTTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTtg                                                                                   <  2:934729/140‑1 (MQ=255)

                                                       cGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTtgctg                                                                                <  2:157724/140‑1 (MQ=255)

                                                          aacaaTCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTgcgc                                                                             <  2:1546097/140‑1 (MQ=255)

                                                            caaTCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCa                                                                           <  1:1268255/140‑1 (MQ=255)

                                                             aaTCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCaa                                                                          <  2:960338/140‑1 (MQ=255)

                                                             aaTCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCaa                                                                          <  1:344292/140‑1 (MQ=255)

                                                              aTCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCaaa                                                                         <  2:490951/140‑1 (MQ=255)

                                                                       ccGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAg                                                                >  2:498951/1‑140 (MQ=255)

                                                                         gAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGcc                                                              <  2:196751/140‑1 (MQ=255)

                                                                           acacCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGa                                                            <  1:1472315/140‑1 (MQ=255)

                                                                           acacCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGa                                                            <  1:1025099/140‑1 (MQ=255)

                                                                                    ccTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGcc                                                              <  2:362275/129‑1 (MQ=255)

                                                                                    ccTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGcc                                                              >  1:362275/1‑129 (MQ=255)

                                                                                    ccTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAg                                                   >  1:1629292/1‑140 (MQ=255)

                                                                                       gtgtCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACa                                                >  2:962926/1‑140 (MQ=255)

                                                                                       gtgtCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACa                                                >  1:1436320/1‑140 (MQ=255)

                                                                                                   aCTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATcg                                    >  2:120140/1‑140 (MQ=255)

                                                                                                    cTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATcgc                                   >  2:1408987/1‑140 (MQ=255)

                                                                                                                tAAGCTGCCTGCATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCttt                       <  2:1503009/140‑1 (MQ=255)

                                                                                                                      gcctgcATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGgcgc                 >  1:1056625/1‑140 (MQ=255)

                                                                                                                      gcctgcATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGgcgc                 >  2:1299500/1‑140 (MQ=255)

                                                                                                                      gcctgcATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGgcgc                 >  1:1286297/1‑140 (MQ=255)

                                                                                                                      gcctgcATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGgcgc                 >  1:263866/1‑140 (MQ=255)

                                                                                                                       cctgcATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGGCGCt                >  1:1671491/1‑140 (MQ=255)

                                                                                                                        ctgcATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGGCGCTa               >  2:1553017/1‑140 (MQ=255)

                                                                                                                        ctgcATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGGCGCTa               >  2:1553016/1‑140 (MQ=255)

                                                                                                                         tgcATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGGCGCTaa              >  2:126480/1‑140 (MQ=255)

                                                                                                                         tgcATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGGCGCTaa              >  2:1142903/1‑140 (MQ=255)

                                                                                                                         tgcATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGGCGCTaa              >  2:286270/1‑140 (MQ=255)

                                                                                                                          gcATCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGGCGCTaaa             <  1:279349/140‑1 (MQ=255)

                                                                                                                            aTCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGGCGCTAAACg           >  2:565847/1‑140 (MQ=255)

                                                                                                                             tCGATAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGGCGCTAAACgg          <  1:591988/140‑1 (MQ=255)

                                                                                                                                 tAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGgcg                  <  1:156277/128‑1 (MQ=255)

                                                                                                                                 tAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGgcg                  >  2:156277/1‑128 (MQ=255)

                                                                                                                                 tAAGGATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGGCGCTAAACGGAAcc      >  2:786227/1‑140 (MQ=255)

                                                                                                                                     gATCGCCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGGCGCTAAACGGAAccgcgc  <  1:1142903/140‑1 (MQ=255)

GCCAGATAAACCGCGGTTCGGTCGGATCTTTAACAAAACGCGCCACGATGGCATTCGCAACAATCACCGCGCCGAACACCGGAACCTGTGTCCAGGCGAACTGCGAAGTTGTTAAGCTGCCTGCATCGATAAGGATCACCGGCGAGACAGCCACCCAGCTCATCATCGGGATATAGCTTAAAGAGATGGTTGCTGCGCCAAAGAGGAACAGCCGATTGCAAAAGACATTACGAAAATCGCGCAAGACGCTTTTGGCGCTAAACGGAACCGCGC  >  NC_000913/4567664‑4567936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A