Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,568,116 C→T G135E (GGG→GAG)  mdtM ← multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,568,1160CT98.8% 282.9 / ‑4.7 82G135E (GGG→GAG) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (35/46);  total (35/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TGAGATAAAACCCATAACCGCAATGATGGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCCCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGCGCAGGCAA  >  NC_000913/4567980‑4568251
                                                                                                                                        |                                                                                                                                       
tGAGATAAAACCCATAACCGCAATGATGGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCtt                                                                                                                                      >  1:1019614/1‑140 (MQ=255)
         aCCCATAACCGCAATGATGGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGTGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTtgtctgtc                                                                                                                             >  2:1131627/1‑140 (MQ=255)
            cATAACCGCAATGATGGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGa                                                                                                                          <  2:355419/140‑1 (MQ=255)
            cATAACCGCAATGATGGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGa                                                                                                                          <  2:261029/140‑1 (MQ=255)
             aTAACCGCAATGATGGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGaa                                                                                                                         >  2:80177/1‑140 (MQ=255)
              tAACCGCAATGATGGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAAc                                                                                                                        >  2:740514/1‑140 (MQ=255)
              tAACCGCAATGATGGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAAc                                                                                                                        <  2:157691/140‑1 (MQ=255)
              tAACCGCAATGATGGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAAc                                                                                                                        >  1:323427/1‑140 (MQ=255)
              tAACCGCAATGATGGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCCCTTTTGTCTATCCGAAc                                                                                                                        <  1:1225378/140‑1 (MQ=255)
                aCCGCAATGATGGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGc                                                                                                                      <  2:100656/140‑1 (MQ=255)
                aCCGCAATGATGGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGc                                                                                                                      <  2:323427/140‑1 (MQ=255)
                  cGCAATGATGGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGcct                                                                                                                    >  2:942062/1‑140 (MQ=255)
                   gCAATGATGGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGcctc                                                                                                                   <  2:763307/140‑1 (MQ=255)
                         atgGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCAc                                                                                                             <  1:618118/140‑1 (MQ=255)
                          tgGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCAcc                                                                                                            <  2:498937/140‑1 (MQ=255)
                            gCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGt                                                                                                          >  1:1025671/1‑140 (MQ=255)
                               aaaaGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGAc                                                                                                       <  1:893393/140‑1 (MQ=255)
                               aaaaGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGAc                                                                                                       <  1:523194/140‑1 (MQ=255)
                               aaaaGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGAc                                                                                                       <  1:786410/140‑1 (MQ=255)
                                    gACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAAc                                                                                                  <  1:860724/140‑1 (MQ=255)
                                      cTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGAc                                                                                                       <  2:1089473/133‑1 (MQ=255)
                                      cTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGAc                                                                                                       >  1:1089473/1‑133 (MQ=255)
                                              tGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGc                                                                                        >  1:1253996/1‑140 (MQ=255)
                                              tGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGc                                                                                        >  2:854776/1‑140 (MQ=255)
                                                 aTAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAAt                                                                                     <  1:15750/140‑1 (MQ=255)
                                                        gCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAg                                                                              >  1:1012946/1‑140 (MQ=255)
                                                        gCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAg                                                                              >  1:929871/1‑140 (MQ=255)
                                                        gCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAg                                                                              >  1:355465/1‑140 (MQ=255)
                                                         cATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGa                                                                             >  2:566810/1‑140 (MQ=255)
                                                         cATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGa                                                                             >  2:853491/1‑140 (MQ=255)
                                                          aTCATAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGAt                                                                            >  1:1064977/1‑140 (MQ=255)
                                                          aTCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGAt                                                                            >  2:397801/1‑140 (MQ=255)
                                                          aTCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGAt                                                                            >  2:907055/1‑140 (MQ=255)
                                                            cAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGTGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATAc                                                                          <  1:1131627/140‑1 (MQ=255)
                                                              gagCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTg                                                                        <  1:382034/140‑1 (MQ=255)
                                                              gagCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTg                                                                        >  2:916147/1‑140 (MQ=255)
                                                               agCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTgg                                                                       >  1:653502/1‑140 (MQ=255)
                                                                gCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGt                                                                      <  2:355465/140‑1 (MQ=255)
                                                                 cTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTg                                                                     >  2:927404/1‑140 (MQ=255)
                                                                 cTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTg                                                                     >  1:147092/1‑140 (MQ=255)
                                                                 cTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTg                                                                     >  2:1229786/1‑140 (MQ=255)
                                                                 cTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTg                                                                     >  1:302195/1‑140 (MQ=255)
                                                                  tGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGc                                                                    <  2:1064977/140‑1 (MQ=255)
                                                                   gcgcCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGcc                                                                   <  2:459029/140‑1 (MQ=255)
                                                                    cgcCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGccc                                                                  >  1:350867/1‑140 (MQ=255)
                                                                    cgcCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGccc                                                                  >  2:130012/1‑140 (MQ=255)
                                                                    cgcCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGcc                                                                   >  2:650787/1‑139 (MQ=255)
                                                                    cgcCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGcc                                                                   <  1:650787/139‑1 (MQ=255)
                                                                      ccGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTg                                                                >  2:958116/1‑140 (MQ=255)
                                                                               gggCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCAcg                                                       <  2:1025671/140‑1 (MQ=255)
                                                                                 gCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCCCCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCAcgcg                                                     <  2:860130/140‑1 (MQ=255)
                                                                                 gCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCAcgcg                                                     <  1:55608/140‑1 (MQ=255)
                                                                                 gCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCAcgcg                                                     <  1:130012/140‑1 (MQ=255)
                                                                                    gATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGa                                                  >  1:695653/1‑140 (MQ=255)
                                                                                      tAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATa                                                <  1:854776/140‑1 (MQ=255)
                                                                                           ggCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGaaa                                           <  2:302195/140‑1 (MQ=255)
                                                                                             cgcAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACt                                         <  2:147092/140‑1 (MQ=255)
                                                                                             cgcAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACt                                         <  1:958116/140‑1 (MQ=255)
                                                                                             cgcAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACt                                         <  1:927404/140‑1 (MQ=255)
                                                                                             cgcAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACt                                         <  2:929871/140‑1 (MQ=255)
                                                                                             cgcAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCATGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACt                                         <  1:740514/140‑1 (MQ=255)
                                                                                                   cAGTACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCa                                   <  2:653502/140‑1 (MQ=255)
                                                                                                      tACGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAg                                <  2:820855/140‑1 (MQ=255)
                                                                                                        cGATGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGAc                              <  1:1229786/140‑1 (MQ=255)
                                                                                                           tGGAGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGtt                           <  2:84848/140‑1 (MQ=255)
                                                                                                              aGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTtgtg                        >  1:757751/1‑140 (MQ=255)
                                                                                                              aGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTtgtg                        >  1:729195/1‑140 (MQ=255)
                                                                                                              aGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTtgtg                        <  2:582425/140‑1 (MQ=255)
                                                                                                              aGGTGATAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTtgtg                        >  1:582425/1‑140 (MQ=255)
                                                                                                                   aTAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAGAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGtt                           >  2:559988/1‑132 (MQ=255)
                                                                                                                   aTAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAGAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGtt                           <  1:559988/132‑1 (MQ=255)
                                                                                                                    tAATCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACAtt                  >  2:269405/1‑140 (MQ=255)
                                                                                                                     aaTCGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTg                 >  1:1089573/1‑140 (MQ=255)
                                                                                                                        cGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTcg              <  1:397801/140‑1 (MQ=255)
                                                                                                                        cGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTcg              <  1:383526/140‑1 (MQ=255)
                                                                                                                        cGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTcg              <  1:1198071/140‑1 (MQ=255)
                                                                                                                        cGCCATCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTcg              <  2:1019614/140‑1 (MQ=255)
                                                                                                                             tCAACTTGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCgcggcg         <  1:269405/140‑1 (MQ=255)
                                                                                                                                  ttGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGCGCAGGc    <  1:907055/140‑1 (MQ=255)
                                                                                                                                   tGATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGCGCAGGCa   <  2:1012946/140‑1 (MQ=255)
                                                                                                                                    gATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGCGCAGGCaa  <  2:757751/140‑1 (MQ=255)
                                                                                                                                    gATCTCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGCGCAGGCaa  <  2:729195/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
TGAGATAAAACCCATAACCGCAATGATGGCAAAAAGGACTTTCCAGTGCATAAAGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCGGCGCAATCAGTACGATGGAGGTGATAATCGCCATCAACTTGATCCCTTTTGTCTGTCCGAACGCCTCCTGCACCGTGACATAACCAACGGTGGCAATAAAACAGATACTGGTGCCCTGAATTGCACGCGCGATAAGAAACTGTGTCATAGACGTTGTGAACATTGTCGCGGCGCAGGCAA  >  NC_000913/4567980‑4568251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A