Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 183 98.6 3427438 96.9% 3321187 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,331 A→G noncoding (71/87 nt) leuQ ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,3310AG98.8% 302.4 / ‑3.5 85noncoding (71/87 nt)leuQtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (45/39);  minor base . (0/1);  total (45/40)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.98e-01

TCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGT  >  NC_000913/4606195‑4606467
                                                                                                                                           |                                                                                                                                        
ccGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCtt                                                                                                                                       <  1:250341/139‑1 (MQ=255)
 cGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGG‑CTTGa                                                                                                                                     <  2:451137/140‑1 (MQ=255)
        tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGAG‑GGGGGGGGGCTTGAAcccc                                                                                                                                >  2:903829/1‑140 (MQ=255)
        tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAAccccc                                                                                                                               >  1:1487543/1‑140 (MQ=255)
         gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCa                                                                                                                              <  1:524291/140‑1 (MQ=255)
          cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCAc                                                                                                                             <  2:462041/140‑1 (MQ=255)
            aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACg                                                                                                                            >  1:83737/1‑139 (MQ=255)
            aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACg                                                                                                                            <  2:83737/139‑1 (MQ=255)
             ggggggggGTTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAAccccc                                                                                                                               <  2:698046/135‑1 (MQ=255)
             ggggggggGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAAccccc                                                                                                                               >  1:698046/1‑135 (MQ=255)
             ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTc                                                                                                                          >  1:685225/1‑140 (MQ=255)
             ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTc                                                                                                                          <  1:755191/140‑1 (MQ=255)
             ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGA‑GCGA‑‑GGGGGGGGGCTTGAACCCCCAcctt                                                                                                                          >  2:1661920/1‑137 (MQ=255)
                gggtgACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCGCCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGt                                                                                                                       <  2:689691/140‑1 (MQ=255)
                  gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTGGA‑GCGA‑‑GGGGGGGGCTTGGAGCCCCCACGTCCgcga                                                                                                                     >  2:1385688/1‑137 (MQ=21)
                  gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGCGCGAGCACTATAAACGAGGTTTTTGGT‑CCGA‑‑GGGGGGGGGCTGTAACCCCCACGTTCTTaa                                                                                                                     >  2:1456645/1‑140 (MQ=18)
                   ggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAg                                                                                                                    <  2:685225/140‑1 (MQ=255)
                   ggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAg                                                                                                                    <  2:509087/140‑1 (MQ=255)
                    gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAgg                                                                                                                   <  1:1035243/140‑1 (MQ=255)
                     aCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGa                                                                                                                  >  2:755191/1‑140 (MQ=255)
                         gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGCACGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACt                                                                                                              >  2:928990/1‑140 (MQ=255)
                         gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGCGGGTCCTGAACCCCCACGGCCGTAAAGACACt                                                                                                              >  2:1476974/1‑140 (MQ=25)
                         gAACCCCCACGTCCGTAAGAACACTAAAACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACt                                                                                                              <  2:895253/140‑1 (MQ=255)
                          aaCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTa                                                                                                             >  1:509087/1‑140 (MQ=255)
                               ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                                                        >  2:1035243/1‑140 (MQ=255)
                                 aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGTGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTg                                                                                                      >  1:1612345/1‑140 (MQ=255)
                                 aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGAGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTg                                                                                                      >  2:1646711/1‑140 (MQ=255)
                                    tCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                                                                                   >  1:1049079/1‑140 (MQ=255)
                                       gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGTGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                                                                                <  2:1612345/140‑1 (MQ=255)
                                             acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                                                                             <  2:59334/137‑1 (MQ=255)
                                             acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                                                                             >  1:59334/1‑137 (MQ=255)
                                                cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                                                                       <  2:239537/140‑1 (MQ=255)
                                                cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                                                                       <  1:1646711/140‑1 (MQ=255)
                                                 tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTAGGA‑GCGA‑‑GGGGGGCGCCTTCAACCCCCACCTCCGAACGGACACTAACCCCTGACTCTAGCGGGTCTAc                                                                                      >  2:498571/1‑140 (MQ=25)
                                                      ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                                                                                 >  2:1058897/1‑140 (MQ=255)
                                                      ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                                                                                 >  1:239537/1‑140 (MQ=255)
                                                          aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                             <  2:1487543/140‑1 (MQ=255)
                                                             cTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                                                                          <  1:32331/140‑1 (MQ=255)
                                                               aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                                                                        >  1:1438611/1‑140 (MQ=255)
                                                               aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGCCTACCAATTCCGCCACCt                                                                        >  1:499872/1‑140 (MQ=255)
                                                                 cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                                       >  2:348610/1‑140 (MQ=255)
                                                                    gTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                                                   <  2:1049079/140‑1 (MQ=255)
                                                                     tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAt                                                                  >  2:1092358/1‑140 (MQ=255)
                                                                             ttCCGCCACCTTCGCCCAGTGCGAGCAAGATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa                                                          <  1:1090224/140‑1 (MQ=255)
                                                                                cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTc                                                       >  2:1091604/1‑140 (MQ=255)
                                                                                  ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCtt                                                     >  2:1611957/1‑140 (MQ=255)
                                                                                  ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCtt                                                     >  1:1603799/1‑140 (MQ=255)
                                                                                    aCCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaa                                                   >  2:1085847/1‑140 (MQ=255)
                                                                                      cTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa                                                 >  1:447177/1‑140 (MQ=255)
                                                                                      cTTCGCCCAGTGCGAGCAATAACAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa                                                 <  1:1603286/140‑1 (MQ=255)
                                                                                        tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGAGGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa                                                 >  1:1385732/1‑140 (MQ=255)
                                                                                        tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAg                                               >  2:1080483/1‑140 (MQ=255)
                                                                                        tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAg                                               >  2:1257125/1‑140 (MQ=255)
                                                                                          gCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGaa                                             <  1:1476974/140‑1 (MQ=255)
                                                                                           cccAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt                                            <  1:1058897/140‑1 (MQ=255)
                                                                                             cAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGAGGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt                                            <  2:1385732/140‑1 (MQ=255)
                                                                                               gTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCt                                        >  2:871747/1‑140 (MQ=255)
                                                                                                tGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTa                                       >  2:142046/1‑140 (MQ=255)
                                                                                                tGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTa                                       >  2:364003/1‑140 (MQ=255)
                                                                                                tGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTa                                       >  1:172284/1‑140 (MQ=255)
                                                                                                tGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTa                                       >  1:721966/1‑140 (MQ=255)
                                                                                                    aGCAATATCAACGTGGTTTTTGGT‑GCGAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCa                                    <  1:348610/140‑1 (MQ=255)
                                                                                                     gCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                                  <  2:1205215/140‑1 (MQ=255)
                                                                                                     gCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                                  >  1:1205215/1‑140 (MQ=255)
                                                                                                     gCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                                  <  1:1080483/140‑1 (MQ=255)
                                                                                                      cAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGCCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACgg                                 <  2:499872/140‑1 (MQ=255)
                                                                                                         tatCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg                              >  1:1234495/1‑140 (MQ=255)
                                                                                                          atCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                                  >  1:339734/1‑135 (MQ=255)
                                                                                                          atCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                                  <  2:339734/135‑1 (MQ=255)
                                                                                                          atCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgc                             >  1:218689/1‑140 (MQ=255)
                                                                                                          atCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgc                             >  2:625128/1‑140 (MQ=255)
                                                                                                           tCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                            <  1:364003/140‑1 (MQ=255)
                                                                                                           tCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                            <  2:721966/140‑1 (MQ=255)
                                                                                                           tCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                            <  2:1603799/140‑1 (MQ=255)
                                                                                                           tCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                            <  1:871747/140‑1 (MQ=255)
                                                                                                           tCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                            <  2:1438611/140‑1 (MQ=255)
                                                                                                           tCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                            <  1:928990/140‑1 (MQ=255)
                                                                                                            cAACGGGGTTTTTGGTGGCAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                            <  2:1570872/140‑1 (MQ=21)
                                                                                                               cGTGGTTTTTGGT‑GCGAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg                               >  1:581418/1‑134 (MQ=255)
                                                                                                               cGTGGTTTTTGGT‑GCGAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg                               <  2:581418/134‑1 (MQ=255)
                                                                                                               cGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCAtt                        >  2:427419/1‑140 (MQ=255)
                                                                                                                 tGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCt                      >  1:248048/1‑140 (MQ=255)
                                                                                                                 tGGTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCt                      >  1:1273127/1‑140 (MQ=255)
                                                                                                                  ggTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTa                     <  2:1273127/140‑1 (MQ=255)
                                                                                                                  ggTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTa                     <  2:248048/140‑1 (MQ=255)
                                                                                                                   gTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAg                    >  2:776682/1‑140 (MQ=255)
                                                                                                                   gTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAg                    >  2:1386031/1‑140 (MQ=255)
                                                                                                                   gTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAg                    >  1:1044665/1‑140 (MQ=255)
                                                                                                                   gTTTTTGGT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAg                    >  1:103761/1‑140 (MQ=255)
                                                                                                                         ggT‑GCGA‑‑GGGGGGGGGCTTGAACCCCCACGTGCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGttt              <  1:498571/140‑1 (MQ=255)
                                                                                                                          gT‑GCGAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGttt              <  1:903829/140‑1 (MQ=255)
                                                                                                                                   ggggggggGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCtt       >  1:198344/1‑140 (MQ=255)
                                                                                                                                    ggggggggCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCttt      >  1:916089/1‑140 (MQ=255)
                                                                                                                                       ggggGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCg   >  2:919730/1‑140 (MQ=255)
                                                                                                                                        ggggCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGt  >  1:1203501/1‑140 (MQ=255)
                                                                                                                                        ggggCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGt  >  2:842014/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                        
TCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGT‑GCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGT  >  NC_000913/4606195‑4606467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A