Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 258 142.3 4824480 99.1% 4781059 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 49,008 A→G T414A (ACC→GCC)  kefC → K(+) : H(+) antiporter KefC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091349,0080AG100.0% 552.2 / NA 153T414A (ACC→GCC) kefCK(+) : H(+) antiporter KefC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (67/86);  total (67/86)

Only 100 of 156 total aligned reads displayed.

TGGCAGCAACGCCGATTCTGCTGGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTACCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCACGCGGATGGATTTACTGGAATCTGC  >  NC_000913/48871‑49144
                                                                                                                                          |                                                                                                                                        
tGGCAGCAACGCCGATTCTGCTGGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGcc                                                                                                                                        >  1:656271/1‑140 (MQ=255)
  gcagcaACGCCGATTCTGCTGGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCgg                                                                                                                                      <  1:1338867/140‑1 (MQ=255)
    agcaACGCCGATTCTGCTGGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGAc                                                                                                                                    >  2:520240/1‑140 (MQ=255)
      caACGCCGATTCTGCTGGTGAATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACg                                                                                                                                   >  2:1407604/1‑140 (MQ=255)
      caACGCCGATTCTGCTGGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGt                                                                                                                                  >  1:752515/1‑140 (MQ=255)
      caACGCCGATTCTGCTGGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGt                                                                                                                                  >  2:566400/1‑140 (MQ=255)
         cGCCGATTCTGCTGGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTa                                                                                                                               <  1:2361487/140‑1 (MQ=255)
         cGCCGATTCTGCTGGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTa                                                                                                                               >  1:1456175/1‑140 (MQ=255)
          gCCGATTCTGCTGGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTAc                                                                                                                              <  1:2128058/140‑1 (MQ=255)
           ccGATTCTGCTGGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACt                                                                                                                             >  1:1922969/1‑140 (MQ=255)
            cGATTCTGCTGGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTg                                                                                                                            >  2:1513891/1‑140 (MQ=255)
                    ctgGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCgg                                                                                                                                      >  1:1667985/1‑122 (MQ=255)
                    ctgGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAg                                                                                                                    >  2:2002249/1‑140 (MQ=255)
                    ctgGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAg                                                                                                                    <  1:1467132/140‑1 (MQ=255)
                    ctgGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAg                                                                                                                    >  1:1006136/1‑140 (MQ=255)
                     tgGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGc                                                                                                                   <  2:2157322/140‑1 (MQ=255)
                     tgGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGc                                                                                                                   <  1:650419/140‑1 (MQ=255)
                     tgGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGc                                                                                                                   <  2:679394/140‑1 (MQ=255)
                       gTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAg                                                                                                                    >  1:2063464/1‑137 (MQ=255)
                       gTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCgg                                                                                                                 <  1:1495050/140‑1 (MQ=255)
                           aTCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGt                                                                                                              <  2:1755073/140‑1 (MQ=255)
                             ccTCAATCGCCTTGAGCAATCTTCTACTTAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTg                                                                                                                            >  1:555314/1‑124 (MQ=255)
                             ccTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCgg                                                                                                                 <  2:585500/135‑1 (MQ=255)
                             ccTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCgg                                                                                                                 >  1:585500/1‑135 (MQ=255)
                             ccTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGa                                                                                                            >  2:1162469/1‑140 (MQ=255)
                                cAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGaaaa                                                                                                         <  2:1220268/140‑1 (MQ=255)
                                cAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGaaaa                                                                                                         <  2:656271/140‑1 (MQ=255)
                                  aTCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAAtg                                                                                                       >  2:1672231/1‑140 (MQ=255)
                                  aTCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAAtg                                                                                                       >  2:303715/1‑140 (MQ=255)
                                  aTCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAAtg                                                                                                       >  2:45791/1‑140 (MQ=255)
                                     gCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAAtggtg                                                                                                    <  2:742298/140‑1 (MQ=255)
                                     gCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAAtggtg                                                                                                    <  2:2195957/140‑1 (MQ=255)
                                     gCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAAtggtg                                                                                                    <  2:623066/140‑1 (MQ=255)
                                      ccTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAAtggtgg                                                                                                   >  1:406860/1‑140 (MQ=255)
                                       cTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATggtggt                                                                                                  >  2:1784598/1‑140 (MQ=255)
                                          gAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAAtggtgg                                                                                                   <  2:966223/136‑1 (MQ=255)
                                          gAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAAtggtgg                                                                                                   >  1:966223/1‑136 (MQ=255)
                                           aGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTc                                                                                              <  1:472064/140‑1 (MQ=255)
                                               aTCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATc                                                                                          <  2:1888380/140‑1 (MQ=255)
                                                tcttctACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCa                                                                                         <  1:2195174/140‑1 (MQ=255)
                                                tcttctACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCa                                                                                         <  1:1162469/140‑1 (MQ=255)
                                                tcttctACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCa                                                                                         <  1:136720/140‑1 (MQ=255)
                                                    ctACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGAt                                                                                     <  1:1784598/140‑1 (MQ=255)
                                                     tACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATc                                                                                    >  1:516240/1‑140 (MQ=255)
                                                       cTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCg                                                                                  <  1:647237/140‑1 (MQ=255)
                                                        tGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATc                                                                                          >  2:271085/1‑131 (MQ=255)
                                                        tGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATc                                                                                          <  1:271085/131‑1 (MQ=255)
                                                          aGGAAGCGCGTGAAGCCGATGAGATCGACGGAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGAc                                                                               <  1:34026/140‑1 (MQ=255)
                                                          aGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGAc                                                                               <  2:2229492/140‑1 (MQ=255)
                                                          aGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGAc                                                                               >  2:740625/1‑140 (MQ=255)
                                                          aGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGAc                                                                               <  1:1024536/140‑1 (MQ=255)
                                                          aGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGAc                                                                               >  1:1033485/1‑140 (MQ=255)
                                                          aGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGAc                                                                               >  2:1024536/1‑140 (MQ=255)
                                                          aGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGAc                                                                               <  2:1642944/140‑1 (MQ=255)
                                                            gAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGACAATGGTGGTACt                                                                                               >  2:1963576/1‑122 (MQ=255)
                                                            gAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCa                                                                             <  1:740625/140‑1 (MQ=255)
                                                            gAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCa                                                                             >  2:548483/1‑140 (MQ=255)
                                                            gAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCa                                                                             <  2:1585256/140‑1 (MQ=255)
                                                                 gcgTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCg                                                                        >  2:642342/1‑140 (MQ=255)
                                                                  cgTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGa                                                                       >  2:1035896/1‑140 (MQ=255)
                                                                  cgTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGa                                                                       >  2:1166792/1‑140 (MQ=255)
                                                                    tGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGCTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGaaa                                                                     >  1:981267/1‑140 (MQ=255)
                                                                          cGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGTAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGc                                                               >  2:1972497/1‑140 (MQ=255)
                                                                             tGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTa                                                            >  2:1764019/1‑140 (MQ=255)
                                                                                 aTCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAAtt                                                        >  1:1724233/1‑140 (MQ=255)
                                                                                 aTCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAAtt                                                        >  1:686014/1‑140 (MQ=255)
                                                                                 aTCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAAtt                                                        <  2:1418495/140‑1 (MQ=255)
                                                                                 aTCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAAtt                                                        <  1:632990/140‑1 (MQ=255)
                                                                                   cgacgaAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTg                                                      >  1:37879/1‑140 (MQ=255)
                                                                                   cgacgaAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTg                                                      <  1:326050/140‑1 (MQ=255)
                                                                                         agaaCAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGa                                                <  2:37879/140‑1 (MQ=255)
                                                                                           aaCAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTTCTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGaaa                                              <  2:2209297/140‑1 (MQ=255)
                                                                                           aaCAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGaaa                                              <  1:247790/140‑1 (MQ=255)
                                                                                           aaCAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGaaa                                              <  2:1681015/140‑1 (MQ=255)
                                                                                           aaCAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGaaa                                              <  2:2331632/140‑1 (MQ=255)
                                                                                              aGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAgtg                                           <  2:2400936/140‑1 (MQ=255)
                                                                                                ccGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGtt                                         >  2:1730671/1‑140 (MQ=255)
                                                                                                ccGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGtt                                         >  2:2057672/1‑140 (MQ=255)
                                                                                                  gcgcgTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGtttt                                       <  2:214916/140‑1 (MQ=255)
                                                                                                   cgcgTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAAtt                                                        <  2:1192399/122‑1 (MQ=255)
                                                                                                   cgcgTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAAtt                                                        >  1:1192399/1‑122 (MQ=255)
                                                                                                   cgcgTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTa                                      >  2:1624879/1‑140 (MQ=255)
                                                                                                     cgTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTa                                                   <  1:1212741/125‑1 (MQ=255)
                                                                                                     cgTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATg                                    >  1:1551230/1‑140 (MQ=255)
                                                                                                            aTCGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGc                             <  2:1595249/140‑1 (MQ=255)
                                                                                                              cGCCGGATTCGGTCGTTTTGGGTAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCa                           <  1:1972497/140‑1 (MQ=255)
                                                                                                              cGCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCa                           <  1:1322177/140‑1 (MQ=255)
                                                                                                               gCCGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCAc                          >  2:906823/1‑140 (MQ=255)
                                                                                                                ccGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCAcg                         <  2:901747/140‑1 (MQ=255)
                                                                                                                 cGGATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCAcgc                        >  2:715931/1‑140 (MQ=255)
                                                                                                                  ggATTCGGTCGTTTTGGGCAGATTGCCGGCCGTTTACTGCTCTCCAGCGGGGTGAAAATGGAGGCACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGCTTTATGGCGATGCCCcgcg                       >  2:821709/1‑140 (MQ=255)
                                                                                                                  ggATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCAcgcg                       <  1:1624879/140‑1 (MQ=255)
                                                                                                                   gATTCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAACTGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCACGCgg                      >  2:1493343/1‑140 (MQ=255)
                                                                                                                     ttCGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCACGCggat                    <  2:1243681/140‑1 (MQ=255)
                                                                                                                       cGGTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCACGCggatgg                  <  2:763534/140‑1 (MQ=255)
                                                                                                                        ggTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCACGCggatgga                 >  2:2064842/1‑140 (MQ=255)
                                                                                                                         gTCGTTTTGGGCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCACGCggatggat                <  1:1005139/140‑1 (MQ=255)
                                                                                                                                 gggCAGATTGCCGGACGTTTACCGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCACGCGGATGGATTTACTGGa        <  1:918212/140‑1 (MQ=255)
                                                                                                                                  ggCAGATTGCCGGACGTTTACTGCTCTCCAGCGGGCTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCACGCGGATGGATTTACTGGaa       <  2:1659455/140‑1 (MQ=255)
                                                                                                                                       aTTGCCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCACGCGGATGGATTTACTGGAATCTGc  >  1:1124913/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                        
TGGCAGCAACGCCGATTCTGCTGGTG‑ATCCTCAATCGCCTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCGCGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTACCGGACGTTTACTGCTCTCCAGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAATTTGGTATGAAAGTGTTTTATGGCGATGCCACGCGGATGGATTTACTGGAATCTGC  >  NC_000913/48871‑49144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A