Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,535,877	G→A	intergenic (‑19/+60)	yddE ← / ← narV	PF02567 family protein YddE/nitrate reductase Z subunit gamma

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,535,877	0	G	A	100.0%	176.2 / NA	50	intergenic (‑19/+60)	yddE/narV	PF02567 family protein YddE/nitrate reductase Z subunit gamma
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/33); total (17/33)

CGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGT  >  NC_000913/1535740‑1536009

cgGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGC‑GGGGGAAAAAAAACCCGCCGAAATTTCGC‑GGAAAGGGTGTGGGGGAAAAAAATCGACGGGGGAGACCTCCGGGTTTCTTTAA‑GTTCTCCTTTGGCTAga                                                                                                                                    >  2:1735401/1‑140 (MQ=255)

 gggCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTagag                                                                                                                                   <  1:512567/140‑1 (MQ=255)

 gggCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTagag                                                                                                                                   <  1:2367507/140‑1 (MQ=255)

   gCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGaa                                                                                                                                 <  2:1715115/140‑1 (MQ=255)

   gCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGaa                                                                                                                                 <  2:2324468/140‑1 (MQ=255)

         aGCTGCATTTGCGCTTCGCTAAGATTATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCa                                                                                                                           <  1:1735401/140‑1 (MQ=255)

             gCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAAACCGGC‑GAAATGCCCC‑GAAAAAGGTGTGAGGGAAAAAAAACGACGTGGGACACCCCC‑GGGTTTATTAACAGTTTCCCTTGACTAAAGAACTATCATaac                                                                                                                       >  2:985372/1‑138 (MQ=255)

             gCATTTGCGCTTCGCTAAGATTATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGc                                                                                                                       <  1:309931/140‑1 (MQ=255)

              cATTTGCGCTTCGCTAAGATTATCGGC‑GGGGGAAACAAAAACCGCAGCATAGTCGCGGGAAGGGTTG‑GAGGGAAAAAGAACTACGTGGGGCACCCCC‑GGGTTTCTTAAAGATCTCCTGTTGGTAGAGGACTATCAtctgt                                                                                                                      >  2:2106564/1‑136 (MQ=255)

                  tGCGCTTCGCTAAGATTATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCa                                                                                                                  <  1:1668352/140‑1 (MQ=255)

                    cgcTTCGCTAAGATTATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAg                                                                                                                <  1:1257422/140‑1 (MQ=255)

                          gCTAAGATTATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCg                                                                                                          <  2:727384/140‑1 (MQ=255)

                           cTAAGATTATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCgg                                                                                                         <  2:1331079/140‑1 (MQ=255)

                            tAAGATTATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGga                                                                                                        <  2:203647/140‑1 (MQ=255)

                              aGATTATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGgaga                                                                                                      <  1:1451885/140‑1 (MQ=255)

                                 ttATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCg                                                                                                   <  1:1393927/140‑1 (MQ=255)

                                 ttATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCg                                                                                                   <  1:30863/140‑1 (MQ=255)

                                   aTCGGC‑GGGGGAAAAAACACCCGCAGAAATGCCGC‑GAAAAGGGTGTGAGGGAAAAACAACGACGGGGGACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGaa                                                                                                 >  2:1537354/1‑140 (MQ=255)

                                          ggggAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTa                                                                                           <  1:2092955/140‑1 (MQ=255)

                                           gggAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTAt                                                                                          <  1:890275/140‑1 (MQ=255)

                                            ggAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATc                                                                                         <  1:547707/140‑1 (MQ=255)

                                                aacaacACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGc                                                                                     <  2:403288/140‑1 (MQ=255)

                                                aacaacACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGc                                                                                     <  1:686706/140‑1 (MQ=255)

                                                        cGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGa                                                                             >  2:693555/1‑140 (MQ=255)

                                                                  gCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATcgcc                                                                   >  1:1623741/1‑140 (MQ=255)

                                                                     gc‑gAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCcgcg                                                                <  2:1623741/140‑1 (MQ=255)

                                                                          aaCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACg                                                            <  1:2321064/140‑1 (MQ=255)

                                                                            cGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGca                                                          <  2:1356909/140‑1 (MQ=255)

                                                                            cGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGca                                                          <  1:2316392/140‑1 (MQ=255)

                                                                            cGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGca                                                          <  1:1979785/140‑1 (MQ=255)

                                                                                  tgAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAAttt                                                    >  1:1695495/1‑140 (MQ=255)

                                                                                   gAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTg                                                   >  2:58172/1‑140 (MQ=255)

                                                                                    aGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGa                                                  >  2:386629/1‑140 (MQ=255)

                                                                                         aaaGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATcg                                             >  2:373618/1‑140 (MQ=255)

                                                                                           aGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATcgac                                           <  1:1359481/140‑1 (MQ=255)

                                                                                               tCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGt                                       >  2:1042291/1‑140 (MQ=255)

                                                                                               tCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGt                                       >  2:1630420/1‑140 (MQ=255)

                                                                                                cGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTa                                      >  2:1743097/1‑140 (MQ=255)

                                                                                                 gACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTaa                                     <  1:1111625/140‑1 (MQ=255)

                                                                                                 gACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTaa                                     >  1:1835082/1‑140 (MQ=255)

                                                                                                             cTGC‑GGTTTCATTAACGTTCTCCTGTGGCTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACg                         >  2:1362664/1‑140 (MQ=255)

                                                                                                              tGC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACgg                        <  1:181511/140‑1 (MQ=255)

                                                                                                               gC‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGgc                       <  1:1230168/140‑1 (MQ=255)

                                                                                                                c‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGgcg                      <  1:1743097/140‑1 (MQ=255)

                                                                                                                c‑GGTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGgcg                      >  1:1449913/1‑140 (MQ=255)

                                                                                                                  ggTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGgcgc                     >  2:30417/1‑140 (MQ=255)

                                                                                                                   gTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTAc                                >  1:663421/1‑128 (MQ=255)

                                                                                                                   gTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTAc                                <  2:663421/128‑1 (MQ=255)

                                                                                                                   gTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGgcgcg                    >  2:2065636/1‑140 (MQ=255)

                                                                                                                   gTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGgcgcg                    >  1:569433/1‑140 (MQ=255)

                                                                                                                   gTTTCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATAACGACGAGTAAAGTACTCAAACGgcgcg                    >  2:2076188/1‑140 (MQ=255)

                                                                                                                      tCATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTc                 <  1:1630420/140‑1 (MQ=255)

                                                                                                                       cATTAACGTTCTCCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATAACGACGAGTAAAGTACTCAAACGGCGCGCTcc                <  1:2076188/140‑1 (MQ=255)

                                                                                                                                 ctcCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCTCACATGCAc      <  1:386629/140‑1 (MQ=255)

                                                                                                                                 ctcCTGTGACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCAc      <  2:1160031/140‑1 (MQ=255)

                                                                                                                                     tgtgACTAGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGt  <  1:1300801/140‑1 (MQ=255)

CGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGC‑GGGGAAAACAACACCGGCAGAATTGCCGC‑GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGC‑GGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGT  >  NC_000913/1535740‑1536009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A