Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,145	G→A	G150S (GGT→AGT)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,145	0	G	A	100.0%	406.9 / NA	113	G150S (GGT→AGT)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (59/54); total (59/54)

Only 100 of 114 total aligned reads displayed.

ATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATT  >  NC_000913/1756010‑1756279

      gCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTggg                                                                                                                              >  1:1242063/1‑140 (MQ=255)

      gCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTggg                                                                                                                              >  2:2295705/1‑140 (MQ=255)

         gTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGa                                                                                                                           <  1:726268/140‑1 (MQ=255)

            aCGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGaaaa                                                                                                                        <  2:282912/140‑1 (MQ=255)

            aCGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGaaaa                                                                                                                        >  1:2188501/1‑140 (MQ=255)

             cGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAAc                                                                                                                       >  1:2223718/1‑140 (MQ=255)

              gTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACa                                                                                                                      >  1:48264/1‑140 (MQ=255)

               tATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACaa                                                                                                                     <  2:2308150/140‑1 (MQ=255)

                 tGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAg                                                                                                                   <  2:1066848/140‑1 (MQ=255)

                        ttCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACaa                                                                                                                     <  2:35980/131‑1 (MQ=255)

                        ttCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACaa                                                                                                                     >  1:35980/1‑131 (MQ=255)

                         tCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAATAGTGACCTGGGCGAAAACAAAGGTGTGAAc                                                                                                           >  2:1475732/1‑140 (MQ=255)

                         tCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGc                                                                                                                             >  1:1042764/1‑122 (MQ=255)

                         tCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGc                                                                                                                             <  2:1042764/122‑1 (MQ=255)

                          cACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAAcc                                                                                                          >  1:284634/1‑140 (MQ=255)

                                tGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGAGTGAAcctgcctg                                                                                                    >  2:809465/1‑140 (MQ=255)

                                 gACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTgg                                                                                                   >  2:766000/1‑140 (MQ=255)

                                    ctgtctgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGt                                                                                                <  2:466925/140‑1 (MQ=255)

                                    ctgtctgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGt                                                                                                <  1:24452/140‑1 (MQ=255)

                                    ctgtctgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGt                                                                                                <  2:2380221/140‑1 (MQ=255)

                                    ctgtctgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGt                                                                                                <  2:362945/140‑1 (MQ=255)

                                       tctgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTc                                                                                             >  2:584212/1‑140 (MQ=255)

                                       tctgtTGGCAACACCGTACTGGTTGACGATGGTCT‑ATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTcc                                                                                            >  2:5056/1‑140 (MQ=255)

                                         tgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCa                                                                                           >  2:1915297/1‑140 (MQ=255)

                                         tgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCa                                                                                           >  2:1907094/1‑140 (MQ=255)

                                           ttGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAAC‑AAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAAC‑AAGGTGTGAACCTGCCTGGCGTTTCCATTGc                                                                                       <  1:1777642/140‑1 (MQ=255)

                                            tGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTg                                                                                        >  2:2236853/1‑140 (MQ=255)

                                            tGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTg                                                                                        <  1:403298/140‑1 (MQ=255)

                                             ggCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGc                                                                                       <  1:1915297/140‑1 (MQ=255)

                                              gCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGct                                                                                      <  1:1759001/140‑1 (MQ=255)

                                              gCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGct                                                                                      <  2:1158363/140‑1 (MQ=255)

                                                 acacCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTg                                                                                   >  2:74160/1‑140 (MQ=255)

                                                       tACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCa                                                                             <  1:960436/140‑1 (MQ=255)

                                                        aCTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCAc                                                                            >  1:1998697/1‑140 (MQ=255)

                                                             ttGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCt                                                                       >  1:207136/1‑140 (MQ=255)

                                                               gACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGa                                                                     >  2:1770444/1‑140 (MQ=255)

                                                               gACGATGGTCT‑ATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGaa                                                                    <  1:5056/140‑1 (MQ=255)

                                                                 cGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGaaa                                                                   <  2:2223718/140‑1 (MQ=255)

                                                                   aTGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGaaaaa                                                                 >  1:2219249/1‑140 (MQ=255)

                                                                      gTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGAc                                                              >  1:250369/1‑140 (MQ=255)

                                                                      gTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGAc                                                              >  2:1538251/1‑140 (MQ=255)

                                                                      gTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCAGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGAc                                                              >  2:2236956/1‑140 (MQ=255)

                                                                       tCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACa                                                             >  1:2366281/1‑140 (MQ=255)

                                                                         tGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAGCAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACaaa                                                           <  2:735857/140‑1 (MQ=255)

                                                                          gATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAAc                                                          <  1:1149501/140‑1 (MQ=255)

                                                                          gATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAAc                                                          <  2:2219249/140‑1 (MQ=255)

                                                                          gATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAAc                                                          <  1:809465/140‑1 (MQ=255)

                                                                           aTCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACa                                                         >  2:1441160/1‑140 (MQ=255)

                                                                            tCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAg                                                        <  1:1907094/140‑1 (MQ=255)

                                                                             cGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAgg                                                       <  2:207136/140‑1 (MQ=255)

                                                                               gTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAc                                                     <  2:1998697/140‑1 (MQ=255)

                                                                               gTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAc                                                     <  1:2295705/140‑1 (MQ=255)

                                                                                tATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAcc                                                    <  1:766000/140‑1 (MQ=255)

                                                                                  tGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAc                                                     <  2:335699/137‑1 (MQ=255)

                                                                                  tGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTg                                                  >  1:777902/1‑140 (MQ=255)

                                                                                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAcc                                                    >  2:1531816/1‑130 (MQ=255)

                                                                                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAcc                                                    <  1:1531816/130‑1 (MQ=255)

                                                                                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAc                                                     >  1:652845/1‑129 (MQ=255)

                                                                                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAc                                                     >  2:129312/1‑129 (MQ=255)

                                                                                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAc                                                     >  1:132629/1‑129 (MQ=255)

                                                                                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAc                                                     <  1:129312/129‑1 (MQ=255)

                                                                                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGAc                                                     <  2:652845/129‑1 (MQ=255)

                                                                                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTgg                                          >  1:1872862/1‑140 (MQ=255)

                                                                                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTgg                                          >  1:1069711/1‑140 (MQ=255)

                                                                                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTgg                                          >  1:1766488/1‑140 (MQ=255)

                                                                                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTgg                                          >  2:185374/1‑140 (MQ=255)

                                                                                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTgg                                          >  2:768930/1‑140 (MQ=255)

                                                                                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTgg                                          >  2:1006582/1‑140 (MQ=255)

                                                                                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTgg                                          >  2:640326/1‑140 (MQ=255)

                                                                                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTgg                                          >  1:1441496/1‑140 (MQ=255)

                                                                                            cGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGtt                                        >  1:2352719/1‑140 (MQ=255)

                                                                                            cGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGtt                                        >  1:1624792/1‑140 (MQ=255)

                                                                                                aTTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGa                                    <  2:284634/140‑1 (MQ=255)

                                                                                                 ttGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGaa                                   >  2:1582134/1‑140 (MQ=255)

                                                                                                  tGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc                                  <  2:1242063/140‑1 (MQ=255)

                                                                                                  tGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc                                  <  1:828288/140‑1 (MQ=255)

                                                                                                  tGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc                                  <  1:1406871/140‑1 (MQ=255)

                                                                                                  tGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc                                  >  1:382024/1‑140 (MQ=255)

                                                                                                   gAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACa                                 <  1:74160/140‑1 (MQ=255)

                                                                                                   gAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACa                                 <  1:1770444/140‑1 (MQ=255)

                                                                                                      ggTAACAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAgg                              >  1:1973922/1‑140 (MQ=255)

                                                                                                         aaCAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGt                           >  2:582316/1‑140 (MQ=255)

                                                                                                         aaCAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGt                           >  2:1582214/1‑140 (MQ=255)

                                                                                                         aaCAAAGTTATCTGTAAAGTGCCGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGt                           >  1:855600/1‑140 (MQ=255)

                                                                                                          aCAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTa                          <  1:1006582/140‑1 (MQ=255)

                                                                                                          aCAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTa                          <  2:382024/140‑1 (MQ=255)

                                                                                                          aCAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTa                          <  1:640326/140‑1 (MQ=255)

                                                                                                           cAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAg                         >  2:428195/1‑140 (MQ=255)

                                                                                                           cAAAGTTATCTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAg                         >  2:777227/1‑140 (MQ=255)

                                                                                                                    cTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTttgttg                <  2:1872862/140‑1 (MQ=255)

                                                                                                                    cTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTttgttg                <  2:1766488/140‑1 (MQ=255)

                                                                                                                    cTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTttgttg                <  1:1475732/140‑1 (MQ=255)

                                                                                                                    cTGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTttgttg                <  1:1582214/140‑1 (MQ=255)

                                                                                                                     tGTAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgc               <  2:1065242/140‑1 (MQ=255)

                                                                                                                       tAAAGTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgctg             <  1:1582134/140‑1 (MQ=255)

                                                                                                                           gTGCTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTc         <  1:1542108/140‑1 (MQ=255)

                                                                                                                              cTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGTCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCtt      >  2:461706/1‑140 (MQ=255)

                                                                                                                              cTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCtt      >  2:1348277/1‑140 (MQ=255)

                                                                                                                              cTGAACAACAGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCtt      >  1:306729/1‑140 (MQ=255)

                                                                                                                                  acaacaGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTAtt  <  1:204681/140‑1 (MQ=255)

ATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATT  >  NC_000913/1756010‑1756279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A