Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 258 142.3 4824480 99.1% 4781059 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,850,034 G→A V400I (GTT→ATT)  sppA → protease IV, a signal peptide peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,850,0340GA100.0% 381.0 / NA 108V400I (GTT→ATT) sppAprotease IV, a signal peptide peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (48/60);  total (48/60)

Only 100 of 109 total aligned reads displayed.

CACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAA  >  NC_000913/1849897‑1850164
                                                                                                                                         |                                                                                                                                  
cacaAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGAtt                                                                                                                                  >  2:550840/1‑140 (MQ=255)
cacaAATCCGCGACACTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGAtt                                                                                                                                  >  2:1192819/1‑140 (MQ=255)
  caAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGTCTGTGATTGt                                                                                                                                >  2:1868144/1‑140 (MQ=255)
     aTCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATc                                                                                                                             >  1:1457037/1‑140 (MQ=255)
     aTCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATc                                                                                                                             >  1:237785/1‑140 (MQ=255)
          cgACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATgg                                                                                                                        >  1:583255/1‑140 (MQ=255)
           gACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATggg                                                                                                                       <  2:514973/140‑1 (MQ=255)
              gCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGggcgg                                                                                                                    <  2:2101598/140‑1 (MQ=255)
               cTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGggcggc                                                                                                                   >  2:1478956/1‑140 (MQ=255)
                  gCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGggcggcatg                                                                                                                <  1:870150/140‑1 (MQ=255)
                     ttGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGggcggcatggcg                                                                                                             <  2:1580797/140‑1 (MQ=255)
                        aCCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGggcggcatggcggca                                                                                                          >  2:1684833/1‑140 (MQ=255)
                            gAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCtg                                                                                                      >  2:1757566/1‑140 (MQ=255)
                             aaaGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCtgg                                                                                                     <  2:1063963/140‑1 (MQ=255)
                              aaGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCtggt                                                                                                    >  1:297070/1‑140 (MQ=255)
                                 tGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTggtggt                                                                                                 <  1:54588/140‑1 (MQ=255)
                                                 gCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGc                                                                                 <  2:1457037/140‑1 (MQ=255)
                                                 gCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGc                                                                                 <  1:630868/140‑1 (MQ=255)
                                                 gCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGc                                                                                 <  1:496626/140‑1 (MQ=255)
                                                 gCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGc                                                                                 >  2:2195097/1‑140 (MQ=255)
                                                 gCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGc                                                                                 <  1:1478956/140‑1 (MQ=255)
                                                 gCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGc                                                                                 <  2:503288/140‑1 (MQ=255)
                                                 gCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGc                                                                                 <  2:510618/140‑1 (MQ=255)
                                                   gtgtTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCa                                                                               <  1:273500/140‑1 (MQ=255)
                                                   gtgtTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCa                                                                               <  1:2404499/140‑1 (MQ=255)
                                                   gtgtTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCa                                                                               <  2:237785/140‑1 (MQ=255)
                                                   gtgtTAATAGCACAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGcaa                                                                               <  1:1534425/140‑3 (MQ=255)
                                                    tgtTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAg                                                                              >  1:2149493/1‑140 (MQ=255)
                                                     gtTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGc                                                                             <  1:82663/140‑1 (MQ=255)
                                                     gtTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGc                                                                             <  2:1446848/140‑1 (MQ=255)
                                                     gtTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGc                                                                             <  1:1991744/140‑1 (MQ=255)
                                                     gtTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGc                                                                             <  2:850836/140‑1 (MQ=255)
                                                       tAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCa                                                                               >  2:1152426/1‑136 (MQ=255)
                                                       tAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCa                                                                               <  1:1152426/136‑1 (MQ=255)
                                                          tAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAAtt                                                                        <  2:392714/140‑1 (MQ=255)
                                                            gCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTAc                                                                      >  1:2053637/1‑140 (MQ=255)
                                                             cccAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACa                                                                     >  1:2127119/1‑140 (MQ=255)
                                                             cccAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACa                                                                     >  1:2037908/1‑140 (MQ=255)
                                                              ccAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACAt                                                                    <  2:1121816/140‑1 (MQ=255)
                                                              ccAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACAt                                                                    <  2:917983/140‑1 (MQ=255)
                                                              ccAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACAt                                                                    >  1:1507724/1‑140 (MQ=255)
                                                              ccAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACAt                                                                    >  1:2287073/1‑140 (MQ=255)
                                                               cAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACAtt                                                                   >  1:1113657/1‑140 (MQ=255)
                                                               cAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACAtt                                                                   <  1:2195097/140‑1 (MQ=255)
                                                               cAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACAtt                                                                   >  1:2221058/1‑140 (MQ=255)
                                                                 ggcggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGCTTTCCACGCCAGCTAATTACATtgt                                                                 >  1:809147/1‑140 (MQ=255)
                                                                 ggcggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACg                                                                                  <  2:410412/123‑1 (MQ=255)
                                                                 ggcggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACg                                                                                  >  1:410412/1‑123 (MQ=255)
                                                                 ggcggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATtgt                                                                 >  1:399724/1‑140 (MQ=255)
                                                                 ggcggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATtgt                                                                 >  2:78300/1‑140 (MQ=255)
                                                                 ggcggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATtgt                                                                 >  1:1709432/1‑140 (MQ=255)
                                                                 ggcggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATtgt                                                                 >  2:1982219/1‑140 (MQ=255)
                                                                 ggcggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATtgt                                                                 >  2:1238402/1‑140 (MQ=255)
                                                                   cggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTgg                                                               <  2:1613464/140‑1 (MQ=255)
                                                                   cggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTgg                                                               <  2:1507724/140‑1 (MQ=255)
                                                                    ggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGc                                                              >  1:1240547/1‑140 (MQ=255)
                                                                     gcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCt                                                             >  1:492587/1‑140 (MQ=255)
                                                                     gcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCt                                                             <  2:2024945/140‑1 (MQ=255)
                                                                      cAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAAtt                                                                        <  1:2128953/128‑1 (MQ=255)
                                                                       aGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCt                                                             <  2:2327978/138‑1 (MQ=255)
                                                                            tACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCa                                                      <  2:297070/140‑1 (MQ=255)
                                                                            tACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCa                                                      <  2:761151/140‑1 (MQ=255)
                                                                            tACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCCGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCa                                                      <  1:1873607/140‑1 (MQ=255)
                                                                                   tCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCt                                               >  2:856426/1‑140 (MQ=255)
                                                                                   tCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCt                                               >  1:1683170/1‑140 (MQ=255)
                                                                                   tCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCt                                               >  2:2055644/1‑140 (MQ=255)
                                                                                     tGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGa                                             <  1:1757566/140‑1 (MQ=255)
                                                                                      gAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGAc                                            <  1:1192819/140‑1 (MQ=255)
                                                                                           gATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGtt                                       >  2:1395826/1‑140 (MQ=255)
                                                                                            aTTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGAc                                            >  1:426782/1‑134 (MQ=255)
                                                                                            aTTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGAc                                            <  2:426782/134‑1 (MQ=255)
                                                                                            aTTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTc                                      >  1:2259572/1‑140 (MQ=255)
                                                                                            aTTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTc                                      >  1:1920795/1‑140 (MQ=255)
                                                                                              tCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTa                                    <  2:3217/140‑1 (MQ=255)
                                                                                                 cgcTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCg                                 >  1:1116512/1‑140 (MQ=255)
                                                                                                 cgcTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCg                                 >  1:1891929/1‑140 (MQ=255)
                                                                                                  gcTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCgg                                <  1:1853680/140‑1 (MQ=255)
                                                                                                       aCTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCt                           <  1:1238402/140‑1 (MQ=255)
                                                                                                          ggCAGCAGCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCg                        <  1:1820689/140‑1 (MQ=255)
                                                                                                            cagcagCCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGc                      <  1:929884/140‑1 (MQ=255)
                                                                                                             agcagcCCGGGCAGCGGATAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCg                     >  1:1118229/1‑140 (MQ=255)
                                                                                                                agcCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGa                  >  1:2099435/1‑140 (MQ=255)
                                                                                                                agcCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGa                  >  2:2333751/1‑140 (MQ=255)
                                                                                                                 gcCCGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGAt                 <  1:881074/140‑1 (MQ=255)
                                                                                                                    cGGGCAGCGGGTAAGTCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATcac              <  1:1868144/140‑1 (MQ=255)
                                                                                                                    cGGGCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATcac              <  2:1240547/140‑1 (MQ=255)
                                                                                                                      ggCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATcacca            <  2:220371/140‑1 (MQ=255)
                                                                                                                      ggCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATcacca            <  1:733242/140‑1 (MQ=255)
                                                                                                                      ggCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATcacca            <  2:2259572/140‑1 (MQ=255)
                                                                                                                      ggCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATcacca            <  1:2055644/140‑1 (MQ=255)
                                                                                                                      ggCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATcacca            <  2:1920795/140‑1 (MQ=255)
                                                                                                                      ggCAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATcacca            <  2:1268168/140‑1 (MQ=255)
                                                                                                                        cAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCaccacc          <  1:2092906/140‑1 (MQ=255)
                                                                                                                        cAGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTAGGCGTGATCaccacc          <  2:1084346/140‑1 (MQ=255)
                                                                                                                         aGCGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCg         <  1:1531464/140‑1 (MQ=255)
                                                                                                                           cGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGc                      <  1:884754/125‑1 (MQ=255)
                                                                                                                           cGGGTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGc                      >  2:884754/1‑125 (MQ=255)
                                                                                                                             ggTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGa     <  2:583255/140‑1 (MQ=255)
                                                                                                                             ggTAAGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGa     <  1:493377/140‑1 (MQ=255)
                                                                                                                                aaGCCTGTGATTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGaaaa  <  2:2053637/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                  
CACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAA  >  NC_000913/1849897‑1850164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A