Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,888,441	T→C	T436A (ACC→GCC)	fadD ←	fatty acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,888,441	0	T	C	100.0%	400.9 / NA	111	T436A (ACC→GCC)	fadD	fatty acyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (55/56); total (55/56)

Only 100 of 113 total aligned reads displayed.

GTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTATCATCATCATCCA  >  NC_000913/1888306‑1888572

        ggATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGcc                                                                                                                         >  1:2232576/1‑140 (MQ=255)

        ggATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGcc                                                                                                                         >  1:1446330/1‑140 (MQ=255)

         gATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCa                                                                                                                        >  1:1176384/1‑140 (MQ=255)

           tgctgcATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCAtt                                                                                                                      >  1:60484/1‑140 (MQ=255)

              tgcATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCAttttt                                                                                                                   >  1:1302365/1‑140 (MQ=255)

                   gacgacATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTtgatga                                                                                                              >  2:1308804/1‑140 (MQ=255)

                     cgacATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGAtt                                                                                                            >  2:205164/1‑140 (MQ=255)

                      gacATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGAttt                                                                                                           <  1:534030/140‑1 (MQ=255)

                       acATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTc                                                                                                          <  1:2160381/140‑1 (MQ=255)

                        cATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCa                                                                                                         >  1:2186646/1‑140 (MQ=255)

                           cTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCg                                                                                                      <  2:1446330/140‑1 (MQ=255)

                            ttCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCgg                                                                                                     <  1:1511162/140‑1 (MQ=255)

                             tCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGt                                                                                                    >  1:955363/1‑140 (MQ=255)

                               aaTCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAg                                                                                                  <  2:565805/140‑1 (MQ=255)

                               aaTCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAg                                                                                                  <  2:1424080/140‑1 (MQ=255)

                                  ctcGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCAt                                                                                               >  1:893084/1‑140 (MQ=255)

                                   tcGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCgg                                                                                                     <  2:1595309/133‑1 (MQ=255)

                                   tcGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATc                                                                                              >  2:1361929/1‑140 (MQ=255)

                                    cGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCg                                                                                             >  2:1251624/1‑140 (MQ=255)

                                      ttGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCggg                                                                                           <  2:2044947/140‑1 (MQ=255)

                                      ttGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCggg                                                                                           >  1:19308/1‑140 (MQ=255)

                                      ttGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCggg                                                                                           <  1:205164/140‑1 (MQ=255)

                                         ggATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACg                                                                                        <  1:1152148/140‑1 (MQ=255)

                                           aTAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCt                                                                                      <  1:1547070/140‑1 (MQ=255)

                                               aCGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCa                                                                                  >  2:916918/1‑140 (MQ=255)

                                                cGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACTATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCCGCCAg                                                                                 <  2:2232576/140‑1 (MQ=255)

                                                   tAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTaa                                                                              >  2:1627076/1‑140 (MQ=255)

                                                    aaaaCCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAAc                                                                             >  1:1097750/1‑140 (MQ=255)

                                                      aaCCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAAccc                                                                           <  2:1176384/140‑1 (MQ=255)

                                                         cGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGc                                                                        <  2:2355715/140‑1 (MQ=255)

                                                         cGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGc                                                                        >  1:1179065/1‑140 (MQ=255)

                                                         cGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGc                                                                        <  2:1302365/140‑1 (MQ=255)

                                                          ggAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCa                                                                       >  1:1028257/1‑140 (MQ=255)

                                                           gAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCAt                                                                      >  2:1869601/1‑140 (MQ=255)

                                                                   aaTCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCg                                                              <  2:2340330/140‑1 (MQ=255)

                                                                   aaTCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCg                                                              <  1:1627076/140‑1 (MQ=255)

                                                                   aaTCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCg                                                              <  1:743072/140‑1 (MQ=255)

                                                                    aTCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCgg                                                             <  1:879354/140‑1 (MQ=255)

                                                                    aTCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCgg                                                             <  1:1251624/140‑1 (MQ=255)

                                                                    aTCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCgg                                                             <  2:2007621/140‑1 (MQ=255)

                                                                    aTCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCgg                                                             <  1:1993613/140‑1 (MQ=255)

                                                                    aTCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCgg                                                             <  2:2172963/140‑1 (MQ=255)

                                                                        tGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCt                                                         >  2:1596304/1‑140 (MQ=255)

                                                                         gTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCtt                                                        >  2:546394/1‑140 (MQ=255)

                                                                         gTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCtt                                                        <  1:1596304/140‑1 (MQ=255)

                                                                          tCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCttt                                                       >  1:1107676/1‑140 (MQ=255)

                                                                          tCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCttt                                                       >  1:506039/1‑140 (MQ=255)

                                                                          tCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCttt                                                       >  1:2136112/1‑140 (MQ=255)

                                                                              ttttACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCCGCCAGTAACCCAGCATCACCTGCGGTCCTTTGaca                                                   <  2:1911153/140‑1 (MQ=255)

                                                                               tttACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGacac                                                  >  1:464599/1‑140 (MQ=255)

                                                                                 tACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACaa                                                >  2:440316/1‑140 (MQ=255)

                                                                                    gATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAg                                              <  1:1862501/139‑1 (MQ=255)

                                                                                    gATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAg                                              >  2:1862501/1‑139 (MQ=255)

                                                                                       cGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCa                                          <  1:1180406/140‑1 (MQ=255)

                                                                                       cGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCa                                          <  1:440316/140‑1 (MQ=255)

                                                                                       cGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCa                                          <  1:1881487/140‑1 (MQ=255)

                                                                                       cGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCa                                          <  2:60484/140‑1 (MQ=255)

                                                                                        gACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCAc                                         >  2:1083373/1‑140 (MQ=255)

                                                                                        gACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCAc                                         >  1:1869276/1‑140 (MQ=255)

                                                                                        gACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCAc                                         <  1:658232/140‑1 (MQ=255)

                                                                                         aCAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCttt                                                       <  1:730792/125‑1 (MQ=255)

                                                                                          cAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCAccc                                       <  2:1541241/140‑1 (MQ=255)

                                                                                            aTGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCgg                                     <  2:2186646/140‑1 (MQ=255)

                                                                                             tGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGt                                    >  1:241656/1‑140 (MQ=255)

                                                                                             tGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGt                                    <  1:484294/140‑1 (MQ=255)

                                                                                             tGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGt                                    <  1:991367/140‑1 (MQ=255)

                                                                                             tGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGt                                    <  2:19308/140‑1 (MQ=255)

                                                                                              gcgcAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGtt                                   <  2:1869276/140‑1 (MQ=255)

                                                                                                gcAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGa                                 <  1:974215/140‑1 (MQ=255)

                                                                                                 cAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGAc                                <  2:955363/140‑1 (MQ=255)

                                                                                                  aGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGAcc                               >  1:420090/1‑140 (MQ=255)

                                                                                                    gAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGAcc                               >  1:246927/1‑138 (MQ=255)

                                                                                                    gAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGAcc                               <  2:246927/138‑1 (MQ=255)

                                                                                                    gAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCtg                             >  2:1483470/1‑140 (MQ=255)

                                                                                                       tCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCtggtg                          <  1:916918/140‑1 (MQ=255)

                                                                                                         cttcttCATCCATTCCCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCAGCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTggtggt                        <  2:932422/140‑1 (MQ=255)

                                                                                                          ttcttcATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTa                       <  2:241656/140‑1 (MQ=255)

                                                                                                            cttcATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACt                     <  1:1308804/140‑1 (MQ=255)

                                                                                                              tcATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTc                   >  2:75228/1‑140 (MQ=255)

                                                                                                              tcATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTc                   >  2:2014690/1‑140 (MQ=255)

                                                                                                              tcATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTc                   <  1:2150361/140‑1 (MQ=255)

                                                                                                              tcATCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTc                   >  1:1589081/1‑140 (MQ=255)

                                                                                                                 tCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCAtt                <  2:1589081/140‑1 (MQ=255)

                                                                                                                 tCCATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCAtt                <  2:1107676/140‑1 (MQ=255)

                                                                                                                  ccATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTa               <  2:918506/140‑1 (MQ=255)

                                                                                                                   cATTACCGTGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTat              >  1:1840434/1‑140 (MQ=255)

                                                                                                                   cATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTat              >  2:302116/1‑140 (MQ=255)

                                                                                                                   cATTACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTat              <  2:605224/140‑1 (MQ=255)

                                                                                                                     ttACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTatca            >  2:892400/1‑140 (MQ=255)

                                                                                                                     ttACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTatca            >  1:1487436/1‑140 (MQ=255)

                                                                                                                     ttACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTatca            >  1:804340/1‑140 (MQ=255)

                                                                                                                      tACCGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTatcat           >  2:350801/1‑140 (MQ=255)

                                                                                                                        ccGCGATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTatcatca         >  2:2173962/1‑140 (MQ=255)

                                                                                                                         cgcgATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTatcatcat        >  2:1930600/1‑140 (MQ=255)

                                                                                                                           cgATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTatcatcatca      <  2:804340/140‑1 (MQ=255)

                                                                                                                           cgATGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTatcatcatca      >  1:2076189/1‑140 (MQ=255)

                                                                                                                              tGTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTATCATCATCATcc   >  2:1201642/1‑140 (MQ=255)

                                                                                                                               gTCGCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTATCATCATCATcca  <  2:420090/140‑1 (MQ=255)

                                                                                                                                  gCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTatcatca         <  1:1143760/130‑1 (MQ=255)

                                                                                                                                  gCCGGCGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTatcatca         >  2:1143760/1‑130 (MQ=255)

GTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGACACAAAGCTCACCCGGTTGACCTGGTGGTACTTCATTATCATCATCATCCA  >  NC_000913/1888306‑1888572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A