Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 258 142.3 4824480 99.1% 4781059 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,339,176 T→C D82G (GAC→GGC)  gyrA ← DNA gyrase subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,339,1760TC99.1% 407.0 / ‑4.7 115D82G (GAC→GGC) gyrADNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (55/59);  total (56/59)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 118 total aligned reads displayed.

GACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGA  >  NC_000913/2339042‑2339311
                                                                                                                                      |                                                                                                                                        
gACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGccacca                                                                                                                                     <  2:2246393/140‑1 (MQ=255)
 aCGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGTCGAGCCACCAt                                                                                                                                    >  2:638788/1‑140 (MQ=255)
 aCGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAt                                                                                                                                    <  2:589811/140‑1 (MQ=255)
     aTTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑gggg                                                                                                                               <  1:2230073/140‑1 (MQ=255)
      tttCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGa                                                                                                                              <  2:898149/140‑1 (MQ=255)
       ttCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGAt                                                                                                                             <  2:507605/140‑1 (MQ=255)
       ttCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGAt                                                                                                                             <  2:524139/140‑1 (MQ=255)
          cGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGt                                                                                                                          <  2:405856/140‑1 (MQ=255)
            tataACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTAt                                                                                                                        >  2:922533/1‑140 (MQ=255)
             ataACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTAtt                                                                                                                       <  1:1348614/140‑1 (MQ=255)
               aaCGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTa                                                                                                                     >  1:1759755/1‑140 (MQ=255)
                 cGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTAcc                                                                                                                   >  1:858606/1‑140 (MQ=255)
                   cATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGAt                                                                                                                             <  2:494813/128‑1 (MQ=255)
                   cATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGAt                                                                                                                             >  1:494813/1‑128 (MQ=255)
                    aTTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGa                                                                                                                              <  1:2116151/126‑1 (MQ=255)
                    aTTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGAt                                                                                                                >  1:626434/1‑140 (MQ=255)
                    aTTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGAt                                                                                                                >  1:1179227/1‑140 (MQ=255)
                    aTTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGAt                                                                                                                <  2:1179227/140‑1 (MQ=255)
                      tGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTa                                                                                                              >  2:623377/1‑140 (MQ=255)
                      tGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTa                                                                                                              >  1:1203821/1‑140 (MQ=255)
                      tGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCAATTa                                                                                                              >  1:304910/1‑140 (MQ=255)
                           ccgcAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCa                                                                                                         >  1:2160142/1‑140 (MQ=255)
                           ccgcAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAACGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCa                                                                                                         >  1:1139286/1‑140 (MQ=255)
                             gcAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGTCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCAcc                                                                                                       <  1:638788/140‑1 (MQ=255)
                             gcAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCAcc                                                                                                       >  2:1991631/1‑140 (MQ=255)
                             gcAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCAcc                                                                                                       <  1:564583/140‑1 (MQ=255)
                              cAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCa                                                                                                      <  2:2308936/140‑1 (MQ=255)
                                         tCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGc                                                                                           <  2:546358/140‑1 (MQ=255)
                                         tCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGc                                                                                           <  2:687445/140‑1 (MQ=255)
                                         tCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGc                                                                                           <  2:679644/140‑1 (MQ=255)
                                         tCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGc                                                                                           <  2:282023/140‑1 (MQ=255)
                                         tCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGc                                                                                           <  1:623377/140‑1 (MQ=255)
                                            aTAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGGCGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGa                                                                                        <  2:1383240/140‑1 (MQ=255)
                                             tAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGAt                                                                                       <  2:510949/140‑1 (MQ=255)
                                                aaCCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGAtttt                                                                                    >  1:1372107/1‑140 (MQ=255)
                                                    gAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTtat                                                                                <  1:1784074/140‑1 (MQ=255)
                                                    gAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTtat                                                                                <  1:1494136/140‑1 (MQ=255)
                                                       gTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAgg                                                                             >  1:1445784/1‑140 (MQ=255)
                                                       gTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAgg                                                                             <  1:935050/140‑1 (MQ=255)
                                                         tACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCATATTTTTTATAGGCt                                                                           <  2:858606/140‑1 (MQ=255)
                                                          aCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGc                                                                                           >  1:1191885/1‑123 (MQ=255)
                                                          aCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGc                                                                                           <  2:1191885/123‑1 (MQ=255)
                                                          aCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAgg                                                                             <  2:215387/137‑1 (MQ=255)
                                                          aCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAgg                                                                             >  1:215387/1‑137 (MQ=255)
                                                               gtccGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTc                                                                     <  1:2395289/138‑1 (MQ=255)
                                                               gACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTc                                                                     <  2:1478589/140‑1 (MQ=255)
                                                                aCCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTcc                                                                    >  2:1861412/1‑140 (MQ=255)
                                                                aCCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTcc                                                                    >  2:2347418/1‑140 (MQ=255)
                                                                aCCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTcc                                                                    <  1:922533/140‑1 (MQ=255)
                                                                    tCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTc                                                                >  1:545443/1‑140 (MQ=255)
                                                                          aGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCAATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGcc                                                          <  2:304910/140‑1 (MQ=255)
                                                                           gCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCt                                                         <  1:1991631/140‑1 (MQ=255)
                                                                            cATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTa                                                        >  1:2391089/1‑140 (MQ=255)
                                                                             atatAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAg                                                       <  1:2017924/140‑1 (MQ=255)
                                                                                  aCGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGt                                                  >  1:1041400/1‑140 (MQ=255)
                                                                                    gCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTc                                                <  2:1372107/140‑1 (MQ=255)
                                                                                    gCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTc                                                <  2:1759755/140‑1 (MQ=255)
                                                                                      aGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCa                                                               >  1:1652918/1‑123 (MQ=255)
                                                                                      aGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCa                                                               <  2:1652918/123‑1 (MQ=255)
                                                                                      aGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGc                                                           >  1:1457669/1‑127 (MQ=255)
                                                                                      aGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGc                                                           <  2:1457669/127‑1 (MQ=255)
                                                                                      aGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCAt                                              >  2:1745027/1‑140 (MQ=255)
                                                                                      aGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCAt                                              >  2:425367/1‑140 (MQ=255)
                                                                                      aGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCAt                                              >  2:850493/1‑140 (MQ=255)
                                                                                      aGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCAt                                              <  1:1861412/140‑1 (MQ=255)
                                                                                       gCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATg                                             >  1:94949/1‑140 (MQ=255)
                                                                                        cGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGcc                                                          <  1:812144/126‑1 (MQ=255)
                                                                                         gagaATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGc                                           >  2:1782534/1‑140 (MQ=255)
                                                                                                  tGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTAc                                  >  2:2167079/1‑140 (MQ=255)
                                                                                                   gcgcCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTAcg                                 <  2:1139286/140‑1 (MQ=255)
                                                                                                   gcgcCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTAcg                                 <  2:2160142/140‑1 (MQ=255)
                                                                                                     gcCATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTAcgcg                               >  2:1436142/1‑140 (MQ=255)
                                                                                                      ccATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGa                              >  2:1231950/1‑140 (MQ=255)
                                                                                                      ccATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGC‑‑ATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACg                            >  1:1782706/1‑140 (MQ=255)
                                                                                                       cATGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGAc                             <  2:94949/140‑1 (MQ=255)
                                                                                                         tGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACgg                           >  1:165658/1‑140 (MQ=255)
                                                                                                         tGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACgg                           >  1:1850898/1‑140 (MQ=255)
                                                                                                         tGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACgg                           >  2:745052/1‑140 (MQ=255)
                                                                                                         tGCGGACGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACgg                           >  1:332933/1‑140 (MQ=255)
                                                                                                              aCGATCGTGTCATAGACCGCCGAGCCACCATGGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTT‑ATGGCGTAAAGTACGCGACGGTGTAc                      >  2:728/1‑140 (MQ=255)
                                                                                                               cGATCGTGTCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTAcc                     <  1:739937/140‑1 (MQ=255)
                                                                                                                      gtCATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGC‑‑ATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAgg            <  2:1782706/140‑1 (MQ=255)
                                                                                                                        cATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTAcc                     >  2:1912344/1‑131 (MQ=255)
                                                                                                                        cATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTAcc                     <  1:1912344/131‑1 (MQ=255)
                                                                                                                        cATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAgg            <  2:626434/140‑1 (MQ=255)
                                                                                                                        cATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAgg            <  1:1058754/140‑1 (MQ=255)
                                                                                                                        cATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAgg            <  1:410637/140‑1 (MQ=255)
                                                                                                                        cATAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAgg            <  2:165658/140‑1 (MQ=255)
                                                                                                                         aTAGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGc           <  2:1041400/140‑1 (MQ=255)
                                                                                                                           aGACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCa         >  2:1658557/1‑140 (MQ=255)
                                                                                                                            gACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCAt        >  1:696218/1‑140 (MQ=255)
                                                                                                                            gACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCAt        >  2:1102609/1‑140 (MQ=255)
                                                                                                                            gACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCAt        >  2:791279/1‑140 (MQ=255)
                                                                                                                            gACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCAt        >  2:1763240/1‑140 (MQ=255)
                                                                                                                            gACCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCAt        >  2:2125770/1‑140 (MQ=255)
                                                                                                                             aCCGCCGAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAgg            >  1:1534737/1‑135 (MQ=255)
                                                                                                                              ccgccgAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCAt        >  2:752354/1‑138 (MQ=255)
                                                                                                                                gccgAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCg    >  2:565752/1‑140 (MQ=255)
                                                                                                                                 ccgAGTCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCgg   >  2:1499065/1‑140 (MQ=255)
                                                                                                                                  cgAGCCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGa  <  2:2136816/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                        
GACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCAT‑GGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGA  >  NC_000913/2339042‑2339311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A