Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,397,693	A→G	S825P (TCA→CCA)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,397,693	0	A	G	98.1%	358.3 / ‑2.7	104	S825P (TCA→CCA)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (43/59); total (44/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

Only 100 of 105 total aligned reads displayed.

CAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGACAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGAT  >  NC_000913/2397559‑2397827

cAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAAtt                                                                                                                                   >  2:355673/1‑140 (MQ=255)

 aGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTc                                                                                                                                  >  1:935406/1‑140 (MQ=255)

   cGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCAt                                                                                                                                <  1:1356373/140‑1 (MQ=255)

   cGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCAt                                                                                                                                <  1:338785/140‑1 (MQ=255)

   cGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCAt                                                                                                                                >  1:320330/1‑140 (MQ=255)

    gTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCAt                                                                                                                                <  1:204140/139‑1 (MQ=255)

    gTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCAt                                                                                                                                >  2:204140/1‑139 (MQ=255)

      gACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGc                                                                                                                             <  1:2228668/140‑1 (MQ=255)

         cGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGc                                                                                                                          <  1:1235907/140‑1 (MQ=255)

          gtgtTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGcc                                                                                                                         <  2:629194/140‑1 (MQ=255)

           tgtTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCa                                                                                                                        >  2:590963/1‑140 (MQ=255)

           ggtTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCa                                                                                                                        <  2:1743448/139‑1 (MQ=255)

             ttGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCaaa                                                                                                                      <  1:518924/140‑1 (MQ=255)

             ttGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCaaa                                                                                                                      >  1:225027/1‑140 (MQ=255)

             ttGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCaaa                                                                                                                      <  1:281235/140‑1 (MQ=255)

              tGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAAc                                                                                                                     <  2:1037010/140‑1 (MQ=255)

               gCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACa                                                                                                                    <  1:332983/140‑1 (MQ=255)

               gCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACa                                                                                                                    <  1:194455/140‑1 (MQ=255)

               gCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACa                                                                                                                    <  1:1920216/140‑1 (MQ=255)

               gCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACa                                                                                                                    <  2:634508/140‑1 (MQ=255)

               gCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACa                                                                                                                    <  2:2297019/140‑1 (MQ=255)

               gCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACa                                                                                                                    <  2:1766863/140‑1 (MQ=255)

                 cATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAgg                                                                                                                  <  1:2281899/140‑1 (MQ=255)

                  aTCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAggt                                                                                                                 <  1:173046/140‑1 (MQ=255)

                   tCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAggtg                                                                                                                >  2:1822268/1‑140 (MQ=255)

                    cGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGA‑CGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAggtggt                                                                                                              >  2:197968/1‑140 (MQ=255)

                     gTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAggtggt                                                                                                              >  1:106811/1‑140 (MQ=255)

                     gTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAggtggt                                                                                                              >  2:2034412/1‑140 (MQ=255)

                     gTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAggtggt                                                                                                              >  2:709278/1‑140 (MQ=255)

                     gTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAggtggt                                                                                                              >  1:1527473/1‑140 (MQ=255)

                     gTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAg                                                                                                                   >  2:603311/1‑135 (MQ=255)

                     gTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAg                                                                                                                   <  1:603311/135‑1 (MQ=255)

                      tAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTa                                                                                                             >  1:2181512/1‑140 (MQ=255)

                            aaGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACgg                                                                                                       <  2:1321118/140‑1 (MQ=255)

                             aGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGgc                                                                                                      >  2:1260793/1‑140 (MQ=255)

                               gagaCGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGgcgc                                                                                                    >  2:824178/1‑140 (MQ=255)

                                  aCGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGAt                                                                                                 >  2:1207767/1‑140 (MQ=255)

                                   cgcgTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATa                                                                                                <  1:2024817/140‑1 (MQ=255)

                                    gcgTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATAc                                                                                               >  2:1469965/1‑140 (MQ=255)

                                     cgTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACg                                                                                              <  2:225027/140‑1 (MQ=255)

                                      gtgtACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGc                                                                                             >  2:1650321/1‑140 (MQ=255)

                                       tgtaggtGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGcc                                                                                            >  2:1596003/7‑140 (MQ=255)

                                       tgtACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGcc                                                                                            <  2:330467/140‑1 (MQ=255)

                                            cTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTc                                                                                       <  1:210050/140‑1 (MQ=255)

                                             tGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTcc                                                                                      <  2:2029746/140‑1 (MQ=255)

                                                 ttCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTc                                                                                  <  1:590963/140‑1 (MQ=255)

                                                   ctcgcccACCTTCGCGGCATCCGCTGGGTTGAGGTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCt                                                                                <  2:1570749/136‑1 (MQ=255)

                                                   cacaCCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATTGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCt                                                                                <  1:2285534/140‑1 (MQ=255)

                                                   cacaCCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCt                                                                                <  2:678526/140‑1 (MQ=255)

                                                    acacCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTg                                                                               <  2:848142/140‑1 (MQ=255)

                                                    acacCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTg                                                                               >  1:848142/1‑140 (MQ=255)

                                                    acacCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTg                                                                               >  1:1727462/1‑140 (MQ=255)

                                                         cAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCt                                                                          <  2:935406/140‑1 (MQ=255)

                                                               cgcgGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGc                                                                    <  1:1469965/140‑1 (MQ=255)

                                                               cgcgGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGc                                                                    >  2:1637365/1‑140 (MQ=255)

                                                                gcgGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCg                                                                   <  1:756541/140‑1 (MQ=255)

                                                                gcgGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCg                                                                   >  1:2254391/1‑140 (MQ=255)

                                                                gcgGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGCGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCg                                                                   >  2:2032101/1‑140 (MQ=255)

                                                                  ggCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTg                                                                 <  1:1272248/140‑1 (MQ=255)

                                                                  ggCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTg                                                                 <  1:341250/140‑1 (MQ=255)

                                                                  ggCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTg                                                                 >  1:1381000/1‑140 (MQ=255)

                                                                    cATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGcc                                                               <  1:1650321/140‑1 (MQ=255)

                                                                      tCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCgg                                                             >  1:943817/1‑140 (MQ=255)

                                                                      tCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCgg                                                             <  1:1246790/140‑1 (MQ=255)

                                                                      tCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCgg                                                             >  2:1575933/1‑140 (MQ=255)

                                                                        cGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTa                                                           <  1:1637365/140‑1 (MQ=255)

                                                                        cGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTa                                                           <  1:531099/140‑1 (MQ=255)

                                                                        cGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTa                                                           <  2:106811/140‑1 (MQ=255)

                                                                         gCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTAc                                                          <  1:1596003/140‑1 (MQ=255)

                                                                           tGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGc                                                        <  2:1527473/140‑1 (MQ=255)

                                                                              gTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTgg                                                     <  2:2181512/140‑1 (MQ=255)

                                                                              gTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTgg                                                     <  1:1993081/140‑1 (MQ=255)

                                                                                tGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTg                                                   <  1:91590/140‑1 (MQ=255)

                                                                                  aGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGaa                                                 >  2:508121/1‑140 (MQ=255)

                                                                                   gTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAg                                                <  1:2034412/140‑1 (MQ=255)

                                                                                   gTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAg                                                <  1:709278/140‑1 (MQ=255)

                                                                                    tttGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGt                                               >  2:1854658/1‑140 (MQ=255)

                                                                                     ttGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTa                                              <  2:2254391/140‑1 (MQ=255)

                                                                                         tGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATcc                                          >  1:662334/1‑140 (MQ=255)

                                                                                            aCGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGa                                       >  1:2230784/1‑140 (MQ=255)

                                                                                            aCGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGa                                       <  1:1335983/140‑1 (MQ=255)

                                                                                              ggCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGAcc                                     <  2:1074553/140‑1 (MQ=255)

                                                                                                cTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCAt                                   >  2:2383270/1‑140 (MQ=255)

                                                                                                 tGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCAtt                                  >  1:1391434/1‑140 (MQ=255)

                                                                                                  gCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCAttt                                 >  1:1167321/1‑140 (MQ=255)

                                                                                                     gCATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCg                              <  2:320330/140‑1 (MQ=255)

                                                                                                      cATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGc                             <  1:1822268/140‑1 (MQ=255)

                                                                                                      cATACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGc                             <  2:1381000/140‑1 (MQ=255)

                                                                                                      cATACGGCTCTGGAAGACCGGAGCACGCTGTGACAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGc                             <  1:954265/140‑1 (MQ=255)

                                                                                                       aTACGGCTCTGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCt                            <  1:2355461/140‑1 (MQ=255)

                                                                                                                tGGAAGACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGGTTCaaa                   >  1:614843/1‑140 (MQ=255)

                                                                                                                     gACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGac              >  1:474590/1‑140 (MQ=255)

                                                                                                                     gACCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGGGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGac              >  2:1857382/1‑140 (MQ=255)

                                                                                                                      aCCGGAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGacg             <  2:662334/140‑1 (MQ=255)

                                                                                                                          gAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGacgcacg         <  1:1857382/140‑1 (MQ=255)

                                                                                                                          gAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGacgcacg         >  1:147712/1‑140 (MQ=255)

                                                                                                                          gAGCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGacgcacg         >  2:1770711/1‑140 (MQ=255)

                                                                                                                            gCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGcc       >  2:103888/1‑140 (MQ=255)

                                                                                                                            gCACGCTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGcc       >  2:903049/1‑140 (MQ=255)

                                                                                                                                 cTGTGGCAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGAt  <  1:628686/140‑1 (MQ=255)

CAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCACGCTGTGACAATTCATCGCTGCCAAACAGGTGGTAATACGGCGCGATACGCCATTTCCCGTCCTGCGGCTGGAAGCGTGCCGGTACGCTGGTGAAGTAATCCAGACCATTTTCGCTGGTTTCAAACAGACGCACGCCCGGAT  >  NC_000913/2397559‑2397827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A