Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,442,175	T→C	C138R (TGC→CGC)	mnmC →	fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,442,175	0	T	C	100.0%	268.2 / NA	75	C138R (TGC→CGC)	mnmC	fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (34/41); total (34/41)

CAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTTGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCTTTGCGCCA  >  NC_000913/2442040‑2442309

cAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCa                                                                                                                                    <  2:651027/140‑1 (MQ=255)

     cTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGtt                                                                                                                               >  1:1150118/1‑140 (MQ=255)

     cTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGtt                                                                                                                               >  2:834915/1‑140 (MQ=255)

       tACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTa                                                                                                                             <  1:615219/140‑1 (MQ=255)

       tACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTa                                                                                                                             >  1:2090327/1‑140 (MQ=255)

       tACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTa                                                                                                                             >  2:1105297/1‑140 (MQ=255)

       tACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCAGTCGCCATCGTTTa                                                                                                                             >  1:575007/1‑140 (MQ=255)

            ttCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCt                                                                                                                        <  1:1002221/140‑1 (MQ=255)

              cATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCG‑TTATTGCTCGa                                                                                                                     >  1:2236412/1‑140 (MQ=255)

                ttAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGAt                                                                                                                    >  1:1454815/1‑140 (MQ=255)

                     tttGAGAAATTTCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAgg                                                                                                               <  1:598883/140‑1 (MQ=255)

                     tttGAGAAATTTCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAgg                                                                                                               <  1:134114/140‑1 (MQ=255)

                          gaAATTTCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCAGTCGCCATCGTTTATTGCTCGATGAAGGCcgcg                                                                                                          <  2:575007/140‑1 (MQ=255)

                          gaAATTTCCCATCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCcgcg                                                                                                          <  2:436571/140‑1 (MQ=255)

                              tttCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGAc                                                                                                      >  2:1039189/1‑140 (MQ=255)

                               ttCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACg                                                                                                     <  1:1556516/140‑1 (MQ=255)

                               ttCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACg                                                                                                     <  1:1117074/140‑1 (MQ=255)

                                tCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGc                                                                                                    <  2:1150118/140‑1 (MQ=255)

                                tCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGc                                                                                                    <  2:733789/140‑1 (MQ=255)

                                 ccccTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCt                                                                                                   <  2:1454815/140‑1 (MQ=255)

                                 ccccTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCt                                                                                                   <  1:90904/140‑1 (MQ=255)

                                       aCCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTg                                                                                                  >  2:886907/1‑135 (MQ=255)

                                       aCCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTg                                                                                                  <  1:886907/135‑1 (MQ=255)

                                       aCCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGAttt                                                                                             >  1:3023/1‑140 (MQ=255)

                                        cccGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTa                                                                                            <  1:2164149/140‑1 (MQ=255)

                                            tGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCt                                                                                                   <  2:466678/129‑1 (MQ=255)

                                            tGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCt                                                                                                   >  1:466678/1‑129 (MQ=255)

                                            tGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGt                                                                                        >  2:1624920/1‑140 (MQ=255)

                                            tGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGt                                                                                        <  1:53846/140‑1 (MQ=255)

                                            tGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGt                                                                                        >  2:740089/1‑140 (MQ=255)

                                            tGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGt                                                                                        >  2:1082979/1‑140 (MQ=255)

                                              cGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGttt                                                                                      >  2:1380408/1‑140 (MQ=255)

                                                gATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTgg                                                                                    >  1:363017/1‑140 (MQ=255)

                                                   ttAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCGCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGa                                                                                 <  1:1950964/140‑1 (MQ=255)

                                                   ttAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGt                                                                                        >  1:2402150/1‑133 (MQ=255)

                                                   ttAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGt                                                                                        <  2:2402150/133‑1 (MQ=255)

                                                   ttAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGa                                                                                 <  2:254646/140‑1 (MQ=255)

                                                   ttAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGa                                                                                 <  2:1346767/140‑1 (MQ=255)

                                                   ttAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGa                                                                                 <  1:1375521/140‑1 (MQ=255)

                                                   ttAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGa                                                                                 <  1:1082979/140‑1 (MQ=255)

                                                     aGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGata                                                                               <  1:834915/140‑1 (MQ=255)

                                                       ccTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATAtt                                                                             >  2:175708/1‑140 (MQ=255)

                                                        cTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTa                                                                            <  1:1105297/140‑1 (MQ=255)

                                                           aGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACg                                                                         >  2:1594352/1‑140 (MQ=255)

                                                            gcgcATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCG‑TTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGaa                                                                       <  2:2236412/140‑1 (MQ=255)

                                                                 tCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGa                                                                   >  2:795450/1‑140 (MQ=255)

                                                                   aaCACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGAcc                                                                 <  2:3023/140‑1 (MQ=255)

                                                                    acacTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCa                                                                <  1:122475/140‑1 (MQ=255)

                                                                        tGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCa                                                            >  1:1728725/1‑140 (MQ=255)

                                                                          gCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAAc                                                          >  2:764139/1‑140 (MQ=255)

                                                                            cGGAACTGGCTCCGTGGGCCGAACAACTTCAGGCGCAGTGGCCAATGCCCTAGCCCGGCCGCCATCGTTTATTGCTCGATGAAGGCCGCGAGACGCTGCATTTATGGCTTGGCGATATTATCGAACTGACCAGCCAACTg                                                        >  2:1727117/1‑140 (MQ=255)

                                                                                    gCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACt                                                         >  2:1979039/1‑131 (MQ=255)

                                                                                    gCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACt                                                         <  1:1979039/131‑1 (MQ=255)

                                                                                    gCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTc                                                >  1:1442270/1‑140 (MQ=255)

                                                                                    gCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTc                                                >  1:2270253/1‑140 (MQ=255)

                                                                                     cTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCg                                               <  2:2270253/140‑1 (MQ=255)

                                                                                       ccGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCt                                             <  2:363017/140‑1 (MQ=255)

                                                                                       ccGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCt                                             <  1:224797/140‑1 (MQ=255)

                                                                                        cGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTa                                            <  1:1107203/140‑1 (MQ=255)

                                                                                        cGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTa                                            <  1:1594352/140‑1 (MQ=255)

                                                                                         gTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTaa                                           >  1:2114809/1‑140 (MQ=255)

                                                                                           gggCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAAt                                         <  2:2090327/140‑1 (MQ=255)

                                                                                            ggCAGAACAACTTCAGGCGCAGTCGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATc                                        >  1:2381900/1‑140 (MQ=255)

                                                                                                  acaacTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAg                                  >  1:208866/1‑140 (MQ=255)

                                                                                                          aGGCGCAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGcc                          <  1:740089/140‑1 (MQ=255)

                                                                                                             cgcAGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTgg                       <  1:1624920/140‑1 (MQ=255)

                                                                                                                aGTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGttt                    >  2:1631295/1‑140 (MQ=255)

                                                                                                                 gTGGCCAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTc                   >  2:1898761/1‑140 (MQ=255)

                                                                                                                     ccAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGa               <  1:1849775/140‑1 (MQ=255)

                                                                                                                      cAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGAc              <  2:208866/140‑1 (MQ=255)

                                                                                                                      cAATGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGAc              <  1:1039189/140‑1 (MQ=255)

                                                                                                                         tGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGc           >  2:1262910/1‑140 (MQ=255)

                                                                                                                         tGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGc           >  1:60865/1‑140 (MQ=255)

                                                                                                                         tGCCCTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGc           >  1:117376/1‑140 (MQ=255)

                                                                                                                            ccTTGCCCGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCttt        >  2:860135/1‑140 (MQ=255)

                                                                                                                                  ccGGTCGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCTTTGCGCCa  <  1:175708/140‑1 (MQ=255)

CAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGCCCTTGCCCGGTTGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTGACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCTTTGCGCCA  >  NC_000913/2442040‑2442309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A