Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,446,579	T→C	T299A (ACC→GCC)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,446,579	0	T	C	100.0%	353.5 / NA	100	T299A (ACC→GCC)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (56/44); total (56/44)

Only 100 of 102 total aligned reads displayed.

TGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCA  >  NC_000913/2446446‑2446713

tGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCt                                                                                                                                  >  2:1747405/1‑140 (MQ=255)

tGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCt                                                                                                                                  >  1:1825719/1‑140 (MQ=255)

tGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCt                                                                                                                                  >  1:1298706/1‑140 (MQ=255)

 gATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTg                                                                                                                                 >  2:570743/1‑140 (MQ=255)

 gATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCGTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAAGGCTg                                                                                                                                 >  2:2215739/1‑140 (MQ=255)

  aTCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTgg                                                                                                                                <  1:109175/140‑1 (MQ=255)

  aTCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTgg                                                                                                                                <  2:1916960/140‑1 (MQ=255)

  aTCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTgg                                                                                                                                <  2:486718/140‑1 (MQ=255)

  aTCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTgg                                                                                                                                <  2:1547585/140‑1 (MQ=255)

  aTCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTgg                                                                                                                                >  1:1411678/1‑140 (MQ=255)

      aTTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGt                                                                                                                            <  2:2275801/140‑1 (MQ=255)

       ttAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTc                                                                                                                           >  2:1199817/1‑140 (MQ=255)

       ttAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTc                                                                                                                           >  2:135835/1‑140 (MQ=255)

       ttAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTc                                                                                                                           <  1:2215739/140‑1 (MQ=255)

       ttAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTc                                                                                                                           >  1:1675364/1‑140 (MQ=255)

         aaaaCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCgg                                                                                                                         >  2:2121661/1‑140 (MQ=255)

           aaCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGtt                                                                                                                       <  2:1298706/140‑1 (MQ=255)

           aaCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGtt                                                                                                                       <  2:1825719/140‑1 (MQ=255)

            aCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGttt                                                                                                                      <  1:111438/140‑1 (MQ=255)

            aCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGttt                                                                                                                      <  1:741797/140‑1 (MQ=255)

              gATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCa                                                                                                                    <  2:1800808/140‑1 (MQ=255)

               aTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAg                                                                                                                   >  1:321573/1‑140 (MQ=255)

               aTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAg                                                                                                                   >  1:90370/1‑140 (MQ=255)

               aTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAg                                                                                                                   >  2:700918/1‑140 (MQ=255)

               aTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAg                                                                                                                   <  2:801714/140‑1 (MQ=255)

                tCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAgc                                                                                                                  <  2:1196756/140‑1 (MQ=255)

                 cGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAgcg                                                                                                                 >  2:2321106/1‑140 (MQ=255)

                 cGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAgcg                                                                                                                 >  1:2045759/1‑140 (MQ=255)

                   ccAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGcc                                                                                                               <  1:1747405/140‑1 (MQ=255)

                   ccAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGcc                                                                                                               <  1:2288035/140‑1 (MQ=255)

                       cATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCatat                                                                                                           <  1:1536961/140‑1 (MQ=255)

                          cGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATggg                                                                                                        <  2:1675364/140‑1 (MQ=255)

                                 gCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGACCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGAt                                                                                                 >  2:28586/1‑140 (MQ=255)

                                    aTCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTtg                                                                                              >  1:1680909/1‑140 (MQ=255)

                                      cGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTtgct                                                                                            <  2:1411678/140‑1 (MQ=255)

                                       ggCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTtgctg                                                                                           >  2:1425688/1‑140 (MQ=255)

                                         cACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGcc                                                                                         <  1:1127872/140‑1 (MQ=255)

                                         cACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGcc                                                                                         <  1:570743/140‑1 (MQ=255)

                                          aCTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGccc                                                                                        <  1:1021755/140‑1 (MQ=255)

                                          aCTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGccc                                                                                        <  1:1971562/140‑1 (MQ=255)

                                           cTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCg                                                                                       <  2:90370/140‑1 (MQ=255)

                                           cTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCg                                                                                       <  2:321573/140‑1 (MQ=255)

                                              cgcgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCgctg                                                                                    >  2:2248310/1‑140 (MQ=255)

                                                   aTCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGAt                                                                               >  1:1623371/1‑140 (MQ=255)

                                                   aTCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGAt                                                                               >  1:951265/1‑140 (MQ=255)

                                                    tCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATa                                                                              <  1:1199817/140‑1 (MQ=255)

                                                    tCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATa                                                                              <  1:135835/140‑1 (MQ=255)

                                                          cacaGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCATTGATTTGCTGCCCGCTGCTGATAccgccg                                                                        >  1:1821510/1‑140 (MQ=255)

                                                          cacaGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATAccgccg                                                                        <  1:1189181/140‑1 (MQ=255)

                                                          cacaGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATAccgccg                                                                        <  2:1680909/140‑1 (MQ=255)

                                                             aGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCgaga                                                                     >  2:1759790/1‑140 (MQ=255)

                                                             aGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCgaga                                                                     >  1:830120/1‑140 (MQ=255)

                                                             aGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCACTGCTGATACCGCCgaga                                                                     >  1:46066/1‑140 (MQ=255)

                                                               ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   >  2:1592442/1‑140 (MQ=255)

                                                               ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   >  2:1178129/1‑140 (MQ=255)

                                                               ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   >  2:2164746/1‑140 (MQ=255)

                                                               ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   <  1:2164746/140‑1 (MQ=255)

                                                               ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   >  1:1637254/1‑140 (MQ=255)

                                                               ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   >  1:1203444/1‑140 (MQ=255)

                                                               ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATCATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   >  2:2128792/1‑140 (MQ=255)

                                                                gATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCCCGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATg                                                                  <  1:2333110/140‑1 (MQ=255)

                                                                  tCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgcc                                                                <  2:1623371/140‑1 (MQ=255)

                                                                     tGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgccgcc                                                             >  2:1294209/1‑140 (MQ=255)

                                                                       aCGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGccgcccg                                                           <  2:951265/140‑1 (MQ=255)

                                                                         ggCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCa                                                         >  1:2276052/1‑140 (MQ=255)

                                                                         ggCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCa                                                         >  2:1270808/1‑140 (MQ=255)

                                                                           ccTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATg                                                       >  2:1759478/1‑140 (MQ=255)

                                                                            cTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATgg                                                      <  2:1637254/140‑1 (MQ=255)

                                                                                     tcatcTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTgg                                             >  2:182263/1‑140 (MQ=255)

                                                                                       atcTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGaa                                           >  2:876282/1‑140 (MQ=255)

                                                                                       atcTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGaa                                           <  1:1759478/140‑1 (MQ=255)

                                                                                         ctcAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAAc                                         <  1:74460/140‑1 (MQ=255)

                                                                                           cAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCg                                       >  2:501495/1‑140 (MQ=255)

                                                                                            aaCTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGt                                      >  1:1540209/1‑140 (MQ=255)

                                                                                              cttcttCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCt                                    <  1:2128792/140‑1 (MQ=255)

                                                                                                  ttcGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTgg                                <  1:1178129/140‑1 (MQ=255)

                                                                                                  ttcGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTgg                                >  2:705920/1‑140 (MQ=255)

                                                                                                   tcGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGaa                                           <  1:2156458/128‑1 (MQ=255)

                                                                                                   tcGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCt                                    <  2:1768557/135‑1 (MQ=255)

                                                                                                   tcGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGt                               >  1:648635/1‑140 (MQ=255)

                                                                                                   tcGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGt                               >  1:1680662/1‑140 (MQ=255)

                                                                                                   tcGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTACCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGaa                                           >  2:2156458/1‑128 (MQ=255)

                                                                                                   tcGCCAAAGCGGTTAATGGAACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCt                                    >  1:1768557/1‑135 (MQ=255)

                                                                                                      ccAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGAt                            <  1:1608358/140‑1 (MQ=255)

                                                                                                            cGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATc                      <  1:501495/140‑1 (MQ=255)

                                                                                                             ggTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATcg                     <  1:1270420/140‑1 (MQ=255)

                                                                                                             ggTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATcg                     <  1:2289634/140‑1 (MQ=255)

                                                                                                             ggTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATcg                     <  2:2276052/140‑1 (MQ=255)

                                                                                                                 aaTGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGt                 >  2:1838907/1‑140 (MQ=255)

                                                                                                                      tACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTgg            >  2:1351284/1‑140 (MQ=255)

                                                                                                                      tACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGGTCTgg            >  2:1138585/1‑140 (MQ=255)

                                                                                                                      gaCGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTgg            >  2:1318031/2‑140 (MQ=255)

                                                                                                                        cGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATc                      >  1:1941765/1‑128 (MQ=255)

                                                                                                                        cGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATc                      <  2:1941765/128‑1 (MQ=255)

                                                                                                                         gACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTg         >  1:1329418/1‑140 (MQ=255)

                                                                                                                           cccGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGcc       >  1:651886/1‑140 (MQ=255)

                                                                                                                           cccGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGcc       >  1:1292143/1‑140 (MQ=255)

                                                                                                                             cggcacggcaATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgc     <  1:28586/140‑1 (MQ=255)

                                                                                                                                cACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCa  >  2:878583/1‑140 (MQ=255)

                                                                                                                                cACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCa  >  2:2080712/1‑140 (MQ=255)

TGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCA  >  NC_000913/2446446‑2446713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A