Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	159	82.1	2830106	99.3%	2810295	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,450,334	G→A	P86L (CCG→CTG)	yfcP ←	putative fimbrial protein YfcP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,450,334	0	G	A	98.6%	248.3 / NA	71	P86L (CCG→CTG)	yfcP	putative fimbrial protein YfcP
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (28/42); minor base T (1/0); total (29/42)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGAT  >  NC_000913/2450198‑2450468

ggCAACCTGCATTTCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGcc                                                                                                                                     >  1:513588/1‑140 (MQ=255)

        gCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCaa                                                                                                                             >  1:686254/1‑140 (MQ=255)

        gCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCaa                                                                                                                             >  1:269054/1‑140 (MQ=255)

         cATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAAt                                                                                                                            >  2:85753/1‑140 (MQ=255)

          aTTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTA‑CCCCGTTATGCTGGCCTTTACCGTGGTTCCCCCCAGCCCTTTGGAATCt                                                                                                                          >  2:1341097/1‑140 (MQ=255)

            tCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCtt                                                                                                                         <  1:1118289/140‑1 (MQ=255)

              cGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTc                                                                                                                       >  1:318394/1‑140 (MQ=255)

              cGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTc                                                                                                                       <  1:1120603/140‑1 (MQ=255)

              cGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTc                                                                                                                       <  1:508714/140‑1 (MQ=255)

                tCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAg                                                                                                                     >  2:1356645/1‑140 (MQ=255)

                     tttttCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTaa                                                                                                                <  2:1262888/140‑1 (MQ=255)

                      ttttCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTaaa                                                                                                               <  2:671878/140‑1 (MQ=255)

                       tttCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAg                                                                                                              <  1:725166/140‑1 (MQ=255)

                        ttCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGa                                                                                                             <  2:1199775/140‑1 (MQ=255)

                         tCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGAc                                                                                                            <  2:333597/140‑1 (MQ=255)

                          ccATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGTAATCTTTCAGCTTAAAGACt                                                                                                           <  1:322750/140‑1 (MQ=255)

                                      ccACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGa                                                                                               <  2:965801/140‑1 (MQ=255)

                                           cccTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGa                                                                                          >  2:437038/1‑140 (MQ=255)

                                           cccTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGa                                                                                          <  2:673105/140‑1 (MQ=255)

                                           cccTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGa                                                                                          >  1:992425/1‑140 (MQ=255)

                                           cccTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGa                                                                                          <  1:433760/140‑1 (MQ=255)

                                           cccTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGa                                                                                          <  1:22603/140‑1 (MQ=255)

                                           cccTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGCATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGa                                                                                          <  2:249624/140‑1 (MQ=255)

                                            ccTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGAc                                                                                         <  2:466972/140‑1 (MQ=255)

                                              tGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTg                                                                                       >  2:1328626/1‑140 (MQ=255)

                                                aGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGc                                                                                     <  1:320570/140‑1 (MQ=255)

                                                     ttGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCa                                                                                >  1:675292/1‑140 (MQ=255)

                                                      tgatgaCGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCaa                                                                               >  2:939266/1‑140 (MQ=255)

                                                      tgatgaCGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCaa                                                                               <  1:963778/140‑1 (MQ=255)

                                                             gTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCaa                                                                        <  2:318394/140‑1 (MQ=255)

                                                               aTCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAAt                                                                      >  1:1365189/1‑140 (MQ=255)

                                                               aTCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAAt                                                                      >  1:609778/1‑140 (MQ=255)

                                                                  cAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCt                                                                   >  1:472324/1‑140 (MQ=255)

                                                                  cAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCt                                                                   <  1:166313/140‑1 (MQ=255)

                                                                     tGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTgac                                                                >  2:360057/1‑140 (MQ=255)

                                                                      gCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCTGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTcacg                                                               >  2:265659/1‑140 (MQ=255)

                                                                       ccAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTgacga                                                              >  1:50218/1‑140 (MQ=255)

                                                                         aGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACt                                                            <  1:131633/140‑1 (MQ=255)

                                                                         aGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACt                                                            <  2:557417/140‑1 (MQ=255)

                                                                                  ggTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTggg                                                   >  1:554614/1‑140 (MQ=255)

                                                                                   gttgttCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGa                                                  <  1:1049119/140‑1 (MQ=255)

                                                                                       ttCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAgc                                              <  1:1328626/140‑1 (MQ=255)

                                                                                          gCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGTGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCa                                           <  2:135023/140‑1 (MQ=255)

                                                                                           cTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCAc                                          <  1:753909/140‑1 (MQ=255)

                                                                                           cTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCAc                                          <  1:437038/140‑1 (MQ=255)

                                                                                                 tctACCCCCTTGAGCGTGCCTTTTACGTTGTATCCCGCCAGCCCTTTGCAATC‑TTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGcc                                   <  2:590971/140‑1 (MQ=255)

                                                                                                  ctACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGcc                                   <  2:423164/140‑1 (MQ=255)

                                                                                                   tACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCa                                  >  1:66270/1‑140 (MQ=255)

                                                                                                        ccGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCAc                                          <  2:125002/127‑1 (MQ=255)

                                                                                                        ccGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCAc                                          >  1:125002/1‑127 (MQ=255)

                                                                                                        ccGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGatat                             >  1:685436/1‑140 (MQ=255)

                                                                                                        ccGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGatat                             >  2:415694/1‑140 (MQ=255)

                                                                                                        ccGTGAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGatat                             >  1:442210/1‑140 (MQ=255)

                                                                                                            gAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGTGCACCTCTGCCAGATATTGGc                         <  2:554614/140‑1 (MQ=255)

                                                                                                            gAGCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGc                         <  1:415694/140‑1 (MQ=255)

                                                                                                              gCGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCa                       <  1:360057/140‑1 (MQ=255)

                                                                                                               cGTGACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACAAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCAt                      >  2:1242408/1‑140 (MQ=255)

                                                                                                                  gACTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATcca                   >  1:848695/1‑140 (MQ=255)

                                                                                                                   aCTTTTACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATccac                  >  2:542278/1‑140 (MQ=255)

                                                                                                                        tACGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATccaccacca             <  2:82414/140‑1 (MQ=255)

                                                                                                                         aCGTTGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAg            <  2:992425/140‑1 (MQ=255)

                                                                                                                             tGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGca        <  2:686254/140‑1 (MQ=255)

                                                                                                                             tGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGca        <  2:848695/140‑1 (MQ=255)

                                                                                                                             tGTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGca        <  1:542278/140‑1 (MQ=255)

                                                                                                                              gTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGGCCGTGGGAAAGCGCTCCTCTGCCAGATATTGGCCATCCACCACCAGCGcac       <  1:1341097/140‑1 (MQ=255)

                                                                                                                              gTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGcac       <  2:66270/140‑1 (MQ=255)

                                                                                                                              gTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGcac       <  2:50218/140‑1 (MQ=255)

                                                                                                                              gTATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGcac       <  1:325997/140‑1 (MQ=255)

                                                                                                                               tATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGcaca      >  2:75978/1‑140 (MQ=255)

                                                                                                                               tATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGca        <  2:258951/138‑1 (MQ=255)

                                                                                                                               tATCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGca        >  1:258951/1‑138 (MQ=255)

                                                                                                                                 tCCCGCCAGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAgg    >  2:241202/1‑140 (MQ=255)

                                                                                                                                   ccgccaGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGAt  <  2:685436/140‑1 (MQ=255)

GGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGAT  >  NC_000913/2450198‑2450468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A