Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,496,504	A→G	Y287C (TAC→TGC)	lpxP →	palmitoleoyl acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,496,504	0	A	G	100.0%	467.8 / NA	130	Y287C (TAC→TGC)	lpxP	palmitoleoyl acyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (68/62); total (68/62)

Only 100 of 132 total aligned reads displayed.

GGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAG  >  NC_000913/2496367‑2496638

ggTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGcc                                                                                                                                        >  2:1850832/1‑140 (MQ=255)

 gTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCct                                                                                                                                       <  2:1409700/140‑1 (MQ=255)

   aaGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCctct                                                                                                                                     <  2:102457/140‑1 (MQ=255)

    aGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTg                                                                                                                                    >  1:768026/1‑140 (MQ=255)

    aGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTg                                                                                                                                    >  2:613220/1‑140 (MQ=255)

              aTTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATNCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGt                                                                                                                         <  1:497543/140‑1 (MQ=255)

              aTTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCTCCGGATCAGTGCCTCTGGAacca‑ccct                                                                                                                         <  2:196949/140‑3 (MQ=255)

              aTTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGt                                                                                                                         <  1:1195553/140‑1 (MQ=255)

                       gATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTaaa                                                                                                                >  1:1407160/1‑140 (MQ=255)

                        atatCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTaaaa                                                                                                               >  1:935454/1‑140 (MQ=255)

                        atatCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTaaaa                                                                                                               >  1:1346637/1‑140 (MQ=255)

                          atCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAAcg                                                                                                             <  2:1161612/140‑1 (MQ=255)

                           tCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAAcgc                                                                                                            <  1:1735988/140‑1 (MQ=255)

                            cGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAAcgcg                                                                                                           <  2:768026/140‑1 (MQ=255)

                            cGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAAcgcg                                                                                                           <  1:92274/140‑1 (MQ=255)

                            cGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAAcgcg                                                                                                           <  2:1137627/140‑1 (MQ=255)

                              tttGTTCATCACCCCAGAGATGGAAGGCTGCCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGcc                                                                                                         <  2:1713667/140‑1 (MQ=255)

                               ttGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGccc                                                                                                        <  1:1417950/140‑1 (MQ=255)

                                 gTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCgg                                                                                                      <  2:1407160/140‑1 (MQ=255)

                                  ttCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGccc                                                                                                        <  2:539548/137‑1 (MQ=255)

                                  ttCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGt                                                                                                     >  2:1499667/1‑140 (MQ=255)

                                     atcaCCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTggg                                                                                                  >  2:1081491/1‑140 (MQ=255)

                                       caCCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGgag                                                                                                >  2:703656/1‑140 (MQ=255)

                                          cccAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAAt                                                                                             <  1:2267525/140‑1 (MQ=255)

                                           ccAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAAtc                                                                                            >  2:314326/1‑140 (MQ=255)

                                            cAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAAcgcg                                                                                                           >  2:64483/1‑124 (MQ=255)

                                            cAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAAtcg                                                                                           >  1:159805/1‑140 (MQ=255)

                                            cAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAAtcg                                                                                           >  1:255736/1‑140 (MQ=255)

                                               agaTGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAAtcgtcg                                                                                        >  2:1844402/1‑140 (MQ=255)

                                                 aTGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGtt                                                                                      <  2:1384516/140‑1 (MQ=255)

                                                 aTGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGtt                                                                                      >  2:2342733/1‑140 (MQ=255)

                                                    gAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGtt                                                                                      <  1:564580/137‑1 (MQ=255)

                                                    gAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGtt                                                                                      >  2:564580/1‑137 (MQ=255)

                                                    gAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTa                                                                                   >  1:567791/1‑140 (MQ=255)

                                                    gAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTa                                                                                   >  1:1668067/1‑140 (MQ=255)

                                                    gAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTa                                                                                   >  2:1564669/1‑140 (MQ=255)

                                                    gAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTa                                                                                   >  1:2267100/1‑140 (MQ=255)

                                                    gAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTa                                                                                   >  2:228871/1‑140 (MQ=255)

                                                     aaGGCTACCGGTCAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTAc                                                                                  <  2:1689436/140‑1 (MQ=255)

                                                     aaGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTAc                                                                                  <  1:1081491/140‑1 (MQ=255)

                                                     aaGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTAc                                                                                  <  1:633285/140‑1 (MQ=255)

                                                        gCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACAtt                                                                               >  1:807914/1‑140 (MQ=255)

                                                         cTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACAttt                                                                              >  1:834054/1‑140 (MQ=255)

                                                         cTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACAttt                                                                              >  1:1631385/1‑140 (MQ=255)

                                                          tACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTa                                                                             >  2:1612412/1‑140 (MQ=255)

                                                          tACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTa                                                                             <  2:1668067/140‑1 (MQ=255)

                                                           aCCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTaa                                                                            >  1:1412465/1‑140 (MQ=255)

                                                            cccGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAAc                                                                           >  1:437789/1‑140 (MQ=255)

                                                               gACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACaaa                                                                        <  1:1527285/140‑1 (MQ=255)

                                                                aCAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAAc                                                                       <  2:266112/140‑1 (MQ=255)

                                                                aCAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAAc                                                                       <  2:2171112/140‑1 (MQ=255)

                                                                aCAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAAc                                                                       <  2:1412465/140‑1 (MQ=255)

                                                                aCAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAAc                                                                       <  2:255736/140‑1 (MQ=255)

                                                                aCAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAAc                                                                       <  1:633393/140‑1 (MQ=255)

                                                                    aTGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGct                                                                   <  2:1259442/140‑1 (MQ=255)

                                                                      gAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGctct                                                                 <  2:2267100/140‑1 (MQ=255)

                                                                      gAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGctct                                                                 <  1:351562/140‑1 (MQ=255)

                                                                          aTCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTAct                                                             <  1:1309520/140‑1 (MQ=255)

                                                                            cAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTActct                                                           >  2:138510/1‑140 (MQ=255)

                                                                                    tGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCcctcc                                                   >  2:263481/1‑140 (MQ=255)

                                                                                            tGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCg                                           <  2:437789/140‑1 (MQ=255)

                                                                                             gAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCgg                                          <  2:807914/140‑1 (MQ=255)

                                                                                             gAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCgg                                          <  1:1545625/140‑1 (MQ=255)

                                                                                             gAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCgg                                          <  1:1593800/140‑1 (MQ=255)

                                                                                             gAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCgg                                          <  1:1499667/140‑1 (MQ=255)

                                                                                             gAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCgg                                          <  1:2352812/140‑1 (MQ=255)

                                                                                              aaCAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGc                                         <  1:490969/140‑1 (MQ=255)

                                                                                              aaCAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGc                                         <  1:2342733/140‑1 (MQ=255)

                                                                                                cAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTcc                                                       <  1:2017145/124‑1 (MQ=255)

                                                                                                cAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTcc                                                       >  2:2017145/1‑124 (MQ=255)

                                                                                                cAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑a                                      >  2:2381478/1‑140 (MQ=255)

                                                                                                 aaGATTATCGAGAAAGAGATCATGCGCGCACCGGCGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑at                                     >  2:778599/1‑140 (MQ=255)

                                                                                                   gATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTGAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ataa                                   >  2:759132/1‑140 (MQ=255)

                                                                                                   gATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ataa                                   >  2:596008/1‑140 (MQ=255)

                                                                                                   gATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ataa                                   >  1:1055337/1‑140 (MQ=255)

                                                                                                    aTTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTTGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCgg                                          >  1:2327159/1‑133 (MQ=255)

                                                                                                    aTTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTTGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCgg                                          <  2:2327159/133‑1 (MQ=255)

                                                                                                    aTTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ataat                                  <  2:1631385/140‑1 (MQ=255)

                                                                                                        tCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCgg                                          >  1:616656/1‑129 (MQ=255)

                                                                                                        tCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCgg                                          <  2:616656/129‑1 (MQ=255)

                                                                                                        tCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAAcc                              >  1:1926420/1‑140 (MQ=255)

                                                                                                        tCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAAcc                              >  2:1217799/1‑140 (MQ=255)

                                                                                                        tCGAGAAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAAcc                              >  1:610047/1‑140 (MQ=255)

                                                                                                           agaAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCACCCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTc                            >  2:1590712/1‑140 (MQ=255)

                                                                                                            gaAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCAt                          <  1:2381478/140‑1 (MQ=255)

                                                                                                            gaAAGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCAt                          <  2:1092628/140‑1 (MQ=255)

                                                                                                             aaaGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCATg                         <  1:766734/140‑1 (MQ=255)

                                                                                                             aaaGAGATCATGCGCGCACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCATg                         >  1:1380969/1‑140 (MQ=255)

                                                                                                                     cATGCGCGCACCGGAGCAGTGCCTCTGGATCCACCCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCATGGTTTTTa                  <  1:1590712/140‑1 (MQ=255)

                                                                                                                         cgcgcACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCATGGTTTTTATGGCt             >  2:1609940/1‑140 (MQ=255)

                                                                                                                           cgcACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCATGGTTTTTATGGCttt           >  2:489435/1‑140 (MQ=255)

                                                                                                                           cgcACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCATGGTTTTTATGGCttt           >  2:2364391/1‑140 (MQ=255)

                                                                                                                           cgcACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCATGGTTTTTATGGCttt           <  1:1933277/140‑1 (MQ=255)

                                                                                                                             cACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCATGGTTTTTGTGGCttttt         >  1:2279094/1‑140 (MQ=255)

                                                                                                                             cACCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCATGGTTTTTATGGCttttt         >  1:1095303/1‑140 (MQ=255)

                                                                                                                              aCCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCATGGTTTTTATGGCtt            >  1:492903/1‑136 (MQ=255)

                                                                                                                              aCCGGAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCATGGTTTTTATGGCtt            <  2:492903/136‑1 (MQ=255)

                                                                                                                                  gAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCATGGTTTTTATGGCTTTTTTCTTa    <  2:610047/140‑1 (MQ=255)

                                                                                                                                  gAGCAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCATGGTTTTTATGGCTTTTTTCTTa    <  2:1895955/140‑1 (MQ=255)

                                                                                                                                     cAGTGCCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTCCCCTCCTCAAATCGGCCAATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAg  >  2:1378368/1‑140 (MQ=255)

GGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCA‑CCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCC‑ATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAG  >  NC_000913/2496367‑2496638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A