Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 258 142.3 4824480 99.1% 4781059 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,811,1340AG100.0% 430.0 / NA 120H122R (CAC→CGC) mprADNA‑binding transcriptional repressor MprA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/74);  total (46/74)

Only 100 of 120 total aligned reads displayed.

AGTTGTGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACA  >  NC_000913/2811001‑2811269
                                                                                                                                         |                                                                                                                                       
aGTTGTGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGtt                                                                                                                                   >  1:2168005/1‑140 (MQ=255)
     tgCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTTGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTgc                                                                                                                              >  1:1450516/1‑140 (MQ=255)
      gCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTgcg                                                                                                                             <  2:310582/140‑1 (MQ=255)
       ctctTGGATCATCCTGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                            >  2:477386/1‑140 (MQ=255)
       ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGATCTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                            <  1:2253882/140‑1 (MQ=255)
       ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                            <  2:1364350/140‑1 (MQ=255)
       ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                            <  1:795100/140‑1 (MQ=255)
       ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                            <  2:598585/140‑1 (MQ=255)
       ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                            <  1:983419/140‑1 (MQ=255)
       ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                            <  1:2112718/140‑1 (MQ=255)
       ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                            <  1:2083628/140‑1 (MQ=255)
       ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                            >  2:860121/1‑140 (MQ=255)
       ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                            <  2:461548/140‑1 (MQ=255)
         ctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGa                                                                                                                          <  2:1841132/140‑1 (MQ=255)
         ctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGa                                                                                                                          <  2:1089816/140‑1 (MQ=255)
         ctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAAATGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGa                                                                                                                          <  2:2251114/140‑1 (MQ=255)
           tGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAgg                                                                                                                        >  1:749986/1‑140 (MQ=255)
            ggATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGt                                                                                                                       >  2:1532486/1‑140 (MQ=255)
               tcatcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGtttt                                                                                                                    >  2:82757/1‑140 (MQ=255)
                 atcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTac                                                                                                                  <  2:1393946/140‑1 (MQ=255)
                  tcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTacc                                                                                                                 >  2:131111/1‑140 (MQ=255)
                  tcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTacc                                                                                                                 >  2:2098740/1‑140 (MQ=255)
                  tcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTacc                                                                                                                 >  2:1053211/1‑140 (MQ=255)
                  tcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTacc                                                                                                                 >  1:2393090/1‑140 (MQ=255)
                  tcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTacc                                                                                                                 >  1:1198273/1‑140 (MQ=255)
                  tcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTacc                                                                                                                 >  1:127758/1‑140 (MQ=255)
                    ccGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTaccac                                                                                                               >  1:233636/1‑140 (MQ=255)
                    ccGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTaccac                                                                                                               <  1:1603040/140‑1 (MQ=255)
                      gTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCg                                                                                                             <  1:1656276/140‑1 (MQ=255)
                      gTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCg                                                                                                             <  2:1198273/140‑1 (MQ=255)
                      gTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCg                                                                                                             <  1:899229/140‑1 (MQ=255)
                      gTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTAGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCg                                                                                                             <  2:176245/140‑1 (MQ=255)
                       tACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCgc                                                                                                            >  1:2331619/1‑140 (MQ=255)
                        aCCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCgca                                                                                                           >  2:1599993/1‑140 (MQ=255)
                           aaCGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCgcagca                                                                                                        >  2:27863/1‑140 (MQ=255)
                            acgcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCAt                                                                                                       >  2:885000/1‑140 (MQ=255)
                             cgcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATa                                                                                                      >  2:742228/1‑140 (MQ=255)
                             cgcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATa                                                                                                      >  2:614911/1‑140 (MQ=255)
                             cgcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATa                                                                                                      >  2:1271255/1‑140 (MQ=255)
                             cgcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATa                                                                                                      >  1:2118731/1‑140 (MQ=255)
                             cgcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATa                                                                                                      >  2:2393664/1‑140 (MQ=255)
                             cgcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATa                                                                                                      >  2:1119707/1‑140 (MQ=255)
                                 acgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActg                                                                                                  >  1:13655/1‑140 (MQ=255)
                                 acgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActg                                                                                                  >  1:1756001/1‑140 (MQ=255)
                                   gcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgcc                                                                                                <  2:912932/140‑1 (MQ=255)
                                   gcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgcc                                                                                                <  1:484996/140‑1 (MQ=255)
                                   gcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgcc                                                                                                <  1:82757/140‑1 (MQ=255)
                                   gcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgcc                                                                                                <  2:1871118/140‑1 (MQ=255)
                                      tATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgcctgc                                                                                             <  1:940402/140‑1 (MQ=255)
                                      tATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCCGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgcctgc                                                                                             <  2:569000/140‑1 (MQ=255)
                                       aTTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgcc                                                                                                <  2:227074/140‑1 (MQ=255)
                                        ttGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCAt                                                                                           <  2:1122998/140‑1 (MQ=255)
                                        ttGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCAt                                                                                           <  1:723207/140‑1 (MQ=255)
                                        ttGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCAt                                                                                           <  1:1532486/140‑1 (MQ=255)
                                        ttGCCGATGAACTGAAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCAt                                                                                           <  1:1022638/140‑1 (MQ=255)
                                             gATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAAct                                                                                      <  2:858356/140‑1 (MQ=255)
                                               tGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAActct                                                                                    >  1:265372/1‑140 (MQ=255)
                                                    tGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTcc                                                                               <  1:1053211/140‑1 (MQ=255)
                                                       aaaaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCcgcg                                                                            <  1:289006/140‑1 (MQ=255)
                                                         aaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCt                                                                          <  2:756655/140‑1 (MQ=255)
                                                         aaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCt                                                                          <  1:1271255/140‑1 (MQ=255)
                                                         aaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGACCGCGCt                                                                          <  2:2393090/140‑1 (MQ=255)
                                                          aaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCG‑‑GCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAg                                                                       <  1:998482/140‑1 (MQ=255)
                                                              cgGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGca                                                                     <  2:233636/140‑1 (MQ=255)
                                                              cgGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGca                                                                     >  1:1705416/1‑140 (MQ=255)
                                                                 ttGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCacaa                                                                  <  2:1705416/140‑1 (MQ=255)
                                                                       cGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGaaa                                                            >  1:1109028/1‑140 (MQ=255)
                                                                         aaCGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGaaaaa                                                          >  2:1430528/1‑140 (MQ=255)
                                                                         aaCGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGaaaaa                                                          >  1:1061467/1‑140 (MQ=255)
                                                                         aaCGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGaaaaa                                                          >  2:2248352/1‑140 (MQ=255)
                                                                           cgtcgtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGa                                                        <  2:1061467/140‑1 (MQ=255)
                                                                           cgtcgtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGa                                                        >  2:1234209/1‑140 (MQ=255)
                                                                            gtcgtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGAt                                                       <  2:13655/140‑1 (MQ=255)
                                                                            gtcgtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGAt                                                       <  2:852705/140‑1 (MQ=255)
                                                                               gtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑G‑AAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGc                                                   >  2:2043131/1‑140 (MQ=255)
                                                                                tGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGAt                                                       >  2:1108143/1‑136 (MQ=255)
                                                                                   aaGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCg                                                <  2:749986/140‑1 (MQ=255)
                                                                                       gATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCa                                            >  2:168968/1‑140 (MQ=255)
                                                                                        aTAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCaa                                           >  2:59202/1‑140 (MQ=255)
                                                                                          aaCGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAAt                                         <  2:951769/140‑1 (MQ=255)
                                                                                                  ccgcTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCa                                 <  2:2331619/140‑1 (MQ=255)
                                                                                                  ccgcTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCa                                 <  1:894496/140‑1 (MQ=255)
                                                                                                  ccgcTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCa                                 <  1:59202/140‑1 (MQ=255)
                                                                                                       gcctgcATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTg                            <  2:265372/140‑1 (MQ=255)
                                                                                                       gcctgcATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTg                            <  2:489783/140‑1 (MQ=255)
                                                                                                         ctgcATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGct                          <  1:108257/140‑1 (MQ=255)
                                                                                                            cATCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCtctc                       <  1:704482/140‑1 (MQ=255)
                                                                                                             aTCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTcc                      >  2:1754818/1‑140 (MQ=255)
                                                                                                             aTCTGCAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTcc                      <  1:1754818/140‑1 (MQ=255)
                                                                                                                  cAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGtct                 >  2:420754/1‑140 (MQ=255)
                                                                                                                  cAATTAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGtct                 <  2:1049367/140‑1 (MQ=255)
                                                                                                                      tAACG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAc             <  1:168968/140‑1 (MQ=255)
                                                                                                                       aaCG‑‑‑‑GAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAcc            >  2:41865/1‑140 (MQ=255)
                                                                                                                          g‑‑‑‑gAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGa         <  1:41865/140‑1 (MQ=255)
                                                                                                                          g‑‑‑‑gAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGa         <  1:433267/140‑1 (MQ=255)
                                                                                                                          g‑‑‑‑gAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGa         <  1:1599993/140‑1 (MQ=255)
                                                                                                                          g‑‑‑‑gAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGa         <  1:1234209/140‑1 (MQ=255)
                                                                                                                                aaaaaGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGa         <  2:270778/138‑1 (MQ=255)
                                                                                                                                aaaaaGGTCGCGAGTTTTTGCGCGAGGCTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGa         >  1:270778/1‑138 (MQ=255)
                                                                                                                                     ggTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACa  <  1:1049162/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
AGTTGTGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACG‑‑‑‑GAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACA  >  NC_000913/2811001‑2811269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A