Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,844,085	G→A	R42R (CGC→CGT)	hycG ←	formate hydrogenlyase subunit HycG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,844,085	0	G	A	98.3%	409.6 / ‑1.3	118	coding (126/768 nt)	hycG	formate hydrogenlyase subunit HycG
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (53/63); minor base . (1/1); total (54/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 118 total aligned reads displayed.

CCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGATGGCCT  >  NC_000913/2843949‑2844220

ccGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACgg                                                                                                                                      <  2:1690938/140‑1 (MQ=255)

ccGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACgg                                                                                                                                      <  1:1201943/140‑1 (MQ=255)

ccGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACgg                                                                                                                                      <  1:697289/140‑1 (MQ=255)

 cgcgCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGt                                                                                                                                     >  1:713807/1‑140 (MQ=255)

  gcgcCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTa                                                                                                                                    <  2:1688426/140‑1 (MQ=255)

     ccGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAAc                                                                                                                                 <  1:1885120/140‑1 (MQ=255)

      cGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACgg                                                                                                                                      <  1:1546699/134‑1 (MQ=255)

      cGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACgg                                                                                                                                      >  2:1546699/1‑134 (MQ=255)

      cGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACa                                                                                                                                <  1:2388271/140‑1 (MQ=255)

      cGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACa                                                                                                                                >  1:413692/1‑140 (MQ=255)

              aGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAg                                                                                                                         <  2:993826/139‑1 (MQ=255)

              aGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAg                                                                                                                         >  1:993826/1‑139 (MQ=255)

                  aaaTATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGt                                                                                                                    >  2:1026195/1‑140 (MQ=255)

                  aaaTATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGt                                                                                                                    >  2:1980245/1‑140 (MQ=255)

                  aaaTATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGt                                                                                                                    >  2:2019695/1‑140 (MQ=255)

                     tatCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGtt                                                                                                                   <  2:1334224/138‑1 (MQ=255)

                       tCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGAt                                                                                                               >  2:1020827/1‑140 (MQ=255)

                        ccGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGAtt                                                                                                              >  1:1913040/1‑140 (MQ=255)

                        ccGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGAtt                                                                                                              >  1:2379247/1‑140 (MQ=255)

                        ccGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGAtt                                                                                                              >  2:134019/1‑140 (MQ=255)

                        ccGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGAtt                                                                                                              >  2:168477/1‑140 (MQ=255)

                        ccGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGACGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCA‑‑CGGTAAACATAGGCAGAACGTTTGAtttt                                                                                                            >  1:2234693/1‑140 (MQ=255)

                          gCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGAtttt                                                                                                            >  2:2364939/1‑140 (MQ=255)

                          gCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGAtttt                                                                                                            >  1:1017015/1‑140 (MQ=255)

                           cATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGAttttt                                                                                                           <  1:2213227/140‑1 (MQ=255)

                               aCGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCa                                                                                                       <  1:168477/140‑1 (MQ=255)

                               aCGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCa                                                                                                       >  2:1329948/1‑140 (MQ=255)

                               aCGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCa                                                                                                       <  1:1329948/140‑1 (MQ=255)

                                     tGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAacg                                                                                                 <  2:1913040/140‑1 (MQ=255)

                                      gAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACATCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAacga                                                                                                <  2:1057701/140‑1 (MQ=255)

                                      gAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAacga                                                                                                >  2:2260536/1‑140 (MQ=255)

                                      gAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAacga                                                                                                <  2:2278172/140‑1 (MQ=255)

                                      gAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAacga                                                                                                <  1:134019/140‑1 (MQ=255)

                                      gAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAacga                                                                                                <  1:384026/140‑1 (MQ=255)

                                        aGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAacgacg                                                                                              >  2:1757870/1‑140 (MQ=255)

                                         ggAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGc                                                                                             >  2:1313521/1‑140 (MQ=255)

                                            aCGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCtt                                                                                          >  2:1950028/1‑140 (MQ=255)

                                               aCTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCAt                                                                                       >  1:1646887/1‑140 (MQ=255)

                                               aCTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCAt                                                                                       >  1:2214541/1‑140 (MQ=255)

                                               aCTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCAt                                                                                       >  1:674213/1‑140 (MQ=255)

                                                cTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATg                                                                                      <  1:1063470/140‑1 (MQ=255)

                                                cTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATg                                                                                      <  2:2270628/140‑1 (MQ=255)

                                                     aTGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGc                                                                                 >  1:1059207/1‑140 (MQ=255)

                                                      tGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCg                                                                                <  1:226085/140‑1 (MQ=255)

                                                      tGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCg                                                                                >  1:761628/1‑140 (MQ=255)

                                                       gCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGa                                                                               <  1:1320517/140‑1 (MQ=255)

                                                       gCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGa                                                                               <  2:280491/140‑1 (MQ=255)

                                                       gCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGa                                                                               <  2:1059207/140‑1 (MQ=255)

                                                       gCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGa                                                                               <  1:1313521/140‑1 (MQ=255)

                                                         cGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATg                                                                             <  2:90975/140‑1 (MQ=255)

                                                         cGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATg                                                                             <  1:909372/140‑1 (MQ=255)

                                                          gAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATgg                                                                            >  2:2051052/1‑140 (MQ=255)

                                                           aaGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGa                                                                           >  2:1293236/1‑140 (MQ=255)

                                                           aaGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGa                                                                           >  1:1399247/1‑140 (MQ=255)

                                                            aGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGAt                                                                          <  2:764075/140‑1 (MQ=255)

                                                                 tCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATc                                                                     <  1:1293236/140‑1 (MQ=255)

                                                                   tGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCa                                                                   <  2:1646887/140‑1 (MQ=255)

                                                                   tGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCa                                                                   <  2:674213/140‑1 (MQ=255)

                                                                   tGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCa                                                                   <  1:2364939/140‑1 (MQ=255)

                                                                     cATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCAcc                                                                 >  1:1684338/1‑140 (MQ=255)

                                                                          aaCAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCAt                                                            >  1:1761399/1‑140 (MQ=255)

                                                                          aaCAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCAt                                                            >  1:1893643/1‑140 (MQ=255)

                                                                           aCAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATg                                                           <  1:1903964/140‑1 (MQ=255)

                                                                               cggcgAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGa                                                       >  1:1952227/1‑140 (MQ=255)

                                                                               cggcgAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGa                                                       >  1:1038415/1‑140 (MQ=255)

                                                                               cggcgAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGa                                                       >  2:789846/1‑140 (MQ=255)

                                                                               cggcgAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGa                                                       >  2:1880126/1‑140 (MQ=255)

                                                                               cggcgAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGa                                                       >  2:352863/1‑140 (MQ=255)

                                                                               cggcgAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGa                                                       >  2:108737/1‑140 (MQ=255)

                                                                                    aaaGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGa                                                  >  2:533766/1‑140 (MQ=255)

                                                                                            ttgcgaaTTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGtt                                          >  1:481420/6‑140 (MQ=255)

                                                                                               aaaaTTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGc                                       <  1:1982181/140‑1 (MQ=255)

                                                                                                    ttcgatttcgCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCAc                                  <  2:2214541/140‑1 (MQ=255)

                                                                                                      cgatttcgCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACgg                                <  1:789846/140‑1 (MQ=255)

                                                                                                      cgatttcgCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACgg                                <  1:1020827/140‑1 (MQ=255)

                                                                                                       gatttcgCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACggg                               <  1:2019695/140‑1 (MQ=255)

                                                                                                        atttcgCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGGATGCCGTTGGCGTCACgggg                              <  2:1269198/140‑1 (MQ=255)

                                                                                                        atttcgCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACgggg                              >  1:1162115/1‑140 (MQ=255)

                                                                                                        atttcgCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACgggg                              <  1:999361/140‑1 (MQ=255)

                                                                                                            cgCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGtt                                          <  2:1521779/124‑1 (MQ=255)

                                                                                                             gCAACCGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCta                         <  2:1952227/140‑1 (MQ=255)

                                                                                                                aCCGTTGCAGCCGCCGCAGTCCA‑‑CGGTAAACATAGGCAGAACGCTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATaaa                    <  2:2234693/140‑1 (MQ=255)

                                                                                                                  cGTTGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATaaa                    <  2:851844/140‑1 (MQ=255)

                                                                                                                     tGCAGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTg                 >  2:353078/1‑140 (MQ=255)

                                                                                                                        aGCCGCCGCAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCtc              <  2:413692/140‑1 (MQ=255)

                                                                                                                            gccgcAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCtcatca          >  1:1556011/1‑140 (MQ=255)

                                                                                                                              cgcAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGa        <  2:1893643/140‑1 (MQ=255)

                                                                                                                              cgcAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGa        <  2:1761399/140‑1 (MQ=255)

                                                                                                                              cgcAGTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGa        <  1:1334283/140‑1 (MQ=255)

                                                                                                                                  gTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGATGGc    >  1:1376053/1‑140 (MQ=255)

                                                                                                                                  gTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGATGGc    <  2:2379247/140‑1 (MQ=255)

                                                                                                                                  gTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGATGGc    <  2:1376053/140‑1 (MQ=255)

                                                                                                                                  gTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGATGGc    <  1:1007557/140‑1 (MQ=255)

                                                                                                                                  gTCCACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGATGGc    <  1:1373911/140‑1 (MQ=255)

                                                                                                                                    ccACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGATGGCct  >  2:2278840/1‑140 (MQ=255)

                                                                                                                                    ccACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGATGGCct  <  2:1803058/140‑1 (MQ=255)

                                                                                                                                    ccACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGATGGCct  <  2:1556011/140‑1 (MQ=255)

                                                                                                                                    ccACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGATGGCct  <  2:1162115/140‑1 (MQ=255)

                                                                                                                                    ccACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGATGGCct  >  1:2258913/1‑140 (MQ=255)

                                                                                                                                    ccACACGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGATGGCct  <  1:2410360/140‑1 (MQ=255)

CCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAACGTTTGATTTTTTTCAGTAACGACGCCTTCATGCTGGCGATGGATTCATCCACCGTCATGGGGACCGGAATGCCGTTGGCGTCACGGGGGCCTAATAAATTGCTCATCAGATGGCCT  >  NC_000913/2843949‑2844220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A