Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,200,205	A→G	V254A (GTG→GCG)	ygiF ←	inorganic triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,200,205	0	A	G	100.0%	456.9 / NA	126	V254A (GTG→GCG)	ygiF	inorganic triphosphatase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (69/57); total (69/57)

Only 100 of 126 total aligned reads displayed.

CCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGT  >  NC_000913/3200068‑3200338

ccgTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGcc                                                                                                                                     >  1:308391/1‑140 (MQ=255)

 cgTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCt                                                                                                                                    >  2:965549/1‑140 (MQ=255)

 cgTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCt                                                                                                                                    >  1:1898945/1‑140 (MQ=255)

          aGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTcg                                                                                                                           <  1:352505/140‑1 (MQ=255)

             cacCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTcgccg                                                                                                                        >  2:2174197/1‑140 (MQ=255)

                cGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCAt                                                                                                                     >  1:1065771/1‑140 (MQ=255)

                cGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCAt                                                                                                                     <  2:1268785/140‑1 (MQ=255)

                cGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCAt                                                                                                                     >  1:1268785/1‑140 (MQ=255)

                  cAGAAGCAATGGGCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCg                                                                                                                   <  2:1422547/140‑1 (MQ=255)

                   aGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGt                                                                                                                  >  2:309852/1‑140 (MQ=255)

                   aGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGt                                                                                                                  >  1:1779913/1‑140 (MQ=255)

                    gAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGtt                                                                                                                 <  2:1898945/140‑1 (MQ=255)

                     aaGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCa                                                                                                                      <  2:2033085/134‑1 (MQ=255)

                     aaGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCa                                                                                                                      >  1:2033085/1‑134 (MQ=255)

                        cAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCcg                                                                                                             <  2:308391/140‑1 (MQ=255)

                        cAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCcg                                                                                                             <  2:1065771/140‑1 (MQ=255)

                        cAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCcg                                                                                                             <  1:1657619/140‑1 (MQ=255)

                          aTGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCcgcg                                                                                                           >  2:411421/1‑140 (MQ=255)

                              tCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTAcc                                                                                                       <  1:965549/140‑1 (MQ=255)

                              tCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTAcc                                                                                                       <  2:826819/140‑1 (MQ=255)

                                gCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCca                                                                                                     <  1:660402/140‑1 (MQ=255)

                                gCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCca                                                                                                     <  1:1972964/140‑1 (MQ=255)

                                 ccTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCcac                                                                                                    >  1:106276/1‑140 (MQ=255)

                                   tCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAg                                                                                                  <  2:2373103/140‑1 (MQ=255)

                                       aTTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGttct                                                                                              >  1:2142318/1‑140 (MQ=255)

                                         tGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGCACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGttcttc                                                                                            <  1:2106098/140‑1 (MQ=255)

                                          gAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCa                                                                                           <  2:2142318/140‑1 (MQ=255)

                                           aGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCAt                                                                                          >  2:1391853/1‑140 (MQ=255)

                                            gTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGttctt                                                                                             <  2:25608/136‑1 (MQ=255)

                                            gTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGttctt                                                                                             >  1:25608/1‑136 (MQ=255)

                                               agcagATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCca                                                                                                     >  1:422214/1‑125 (MQ=255)

                                               agcagATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCca                                                                                                     <  2:422214/125‑1 (MQ=255)

                                               agcagATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATa                                                                                      >  1:1239309/1‑140 (MQ=255)

                                                 cagATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCACATCGTTGCCGCGTACCCACAGTTCTTCATGATACt                                                                                    >  1:1304755/1‑140 (MQ=255)

                                                 cagATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACAGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACt                                                                                    >  2:1172522/1‑140 (MQ=255)

                                                   gATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGc                                                                                  >  1:1417173/1‑140 (MQ=255)

                                                     tCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACTGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCa                                                                                >  2:1923259/1‑140 (MQ=255)

                                                       aCGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCAtt                                                                              <  1:12057/140‑1 (MQ=255)

                                                       aCGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCAtt                                                                              <  1:411421/140‑1 (MQ=255)

                                                       aCGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACAGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCAtt                                                                              <  1:1172522/140‑1 (MQ=255)

                                                         gTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTgc                                                                            >  1:2139/1‑140 (MQ=255)

                                                                  tgCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGcc                                                                   >  1:2317086/1‑140 (MQ=255)

                                                                  tgCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGcc                                                                   >  2:719858/1‑140 (MQ=255)

                                                                   gCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCa                                                                  <  1:1113660/140‑1 (MQ=255)

                                                                    cTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAg                                                                 >  1:1314563/1‑140 (MQ=255)

                                                                             cgcgGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAgcgc                                                        <  2:346002/140‑1 (MQ=255)

                                                                             cgcgGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAgcgc                                                        <  2:200360/140‑1 (MQ=255)

                                                                             cgcgGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAgcgc                                                        <  2:1865906/140‑1 (MQ=255)

                                                                               cgGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGcgccg                                                      <  2:337791/140‑1 (MQ=255)

                                                                                 gCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCt                                                    >  2:2228308/1‑140 (MQ=255)

                                                                                  cacaATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCtt                                                   >  2:1275613/1‑140 (MQ=255)

                                                                                   acaATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTc                                                  >  1:2354007/1‑140 (MQ=255)

                                                                                       tACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGc                                              >  2:1014133/1‑140 (MQ=255)

                                                                                       tACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGc                                              >  2:2051665/1‑140 (MQ=255)

                                                                                         ccaccGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGccc                                            <  1:719858/140‑1 (MQ=255)

                                                                                         ccaccGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGccc                                            <  1:2228308/140‑1 (MQ=255)

                                                                                            ccGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAg                                               >  2:497736/1‑134 (MQ=255)

                                                                                            ccGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAg                                               <  1:497736/134‑1 (MQ=255)

                                                                                            ccGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTg                                         >  1:877585/1‑140 (MQ=255)

                                                                                            ccGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTg                                         >  1:1517558/1‑140 (MQ=255)

                                                                                            ccGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTg                                         >  1:2118905/1‑140 (MQ=255)

                                                                                            ccGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTg                                         >  2:843412/1‑140 (MQ=255)

                                                                                              gAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGtt                                       <  2:106276/140‑1 (MQ=255)

                                                                                              gAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGtt                                       <  1:1391853/140‑1 (MQ=255)

                                                                                               aaCAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTc                                      >  2:308611/1‑140 (MQ=255)

                                                                                               aaCAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTc                                      >  2:1103560/1‑140 (MQ=255)

                                                                                                 cAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCca                                    >  2:537633/1‑140 (MQ=255)

                                                                                                 cAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCca                                    >  1:920290/1‑140 (MQ=255)

                                                                                                 cAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCca                                    >  1:1561936/1‑140 (MQ=255)

                                                                                                         gCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCt                            <  1:670975/140‑1 (MQ=255)

                                                                                                         gCGTATGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTCCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCt                            <  2:2118905/140‑1 (MQ=255)

                                                                                                              tGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGc                       >  1:1075371/1‑140 (MQ=255)

                                                                                                              tGACGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGc                       >  2:615119/1‑140 (MQ=255)

                                                                                                                aCGGACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCg                     <  2:1413559/140‑1 (MQ=255)

                                                                                                                  ggACCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCa                   <  1:2174197/140‑1 (MQ=255)

                                                                                                                    aCCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCtt                           <  2:672195/130‑1 (MQ=255)

                                                                                                                    aCCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCtt                           >  1:672195/1‑130 (MQ=255)

                                                                                                                    aCCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAAc                 <  2:2317086/140‑1 (MQ=255)

                                                                                                                    aCCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAAc                 >  1:1944248/1‑140 (MQ=255)

                                                                                                                    aCCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAAc                 >  2:2378612/1‑140 (MQ=255)

                                                                                                                    aCCAGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAAc                 <  2:1314563/140‑1 (MQ=255)

                                                                                                                       aGGCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATg              <  2:680247/140‑1 (MQ=255)

                                                                                                                        ggCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGc             >  1:1579476/1‑140 (MQ=255)

                                                                                                                        ggCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGc             >  2:1026032/1‑140 (MQ=255)

                                                                                                                         gCTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCa            <  1:843412/140‑1 (MQ=255)

                                                                                                                          cTAATGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCaa           <  2:2345628/140‑1 (MQ=255)

                                                                                                                              tGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAAtg       >  1:1583310/1‑140 (MQ=255)

                                                                                                                              tGGCTGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAAtg       >  1:1905284/1‑140 (MQ=255)

                                                                                                                                  tGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAAtggtgg   <  2:1779913/140‑1 (MQ=255)

                                                                                                                                  tGCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAAtggtgg   >  2:1461741/1‑140 (MQ=255)

                                                                                                                                   gCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGc                    >  1:667518/1‑122 (MQ=255)

                                                                                                                                   gCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGc                    <  2:667518/122‑1 (MQ=255)

                                                                                                                                   gCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATggtggt  >  2:679505/1‑140 (MQ=255)

                                                                                                                                   gCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATggtggt  >  1:1034102/1‑140 (MQ=255)

                                                                                                                                   gCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATggtggt  >  2:153937/1‑140 (MQ=255)

                                                                                                                                   gCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATggtggt  <  1:153937/140‑1 (MQ=255)

                                                                                                                                   gCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATggtggt  >  1:440225/1‑140 (MQ=255)

                                                                                                                                   gCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATggtggt  >  1:286378/1‑140 (MQ=255)

                                                                                                                                   gCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATggtggt  >  1:2262640/1‑140 (MQ=255)

                                                                                                                                   gCCAGCGCCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATggtggt  <  1:1923259/140‑1 (MQ=255)

CCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCCGCAACATGCAAAATGGTGGT  >  NC_000913/3200068‑3200338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A