Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,201,476	C→T	S90S (AGC→AGT)	ygiM →	putative signal transduction protein (SH3 domain)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,201,476	0	C	T	100.0%	229.3 / NA	65	S90S (AGC→AGT)	ygiM	putative signal transduction protein (SH3 domain)
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (32/33); total (32/33)

CCTCGTGGGCACGGTTAACGCCGGCGAGGAAGTGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAA  >  NC_000913/3201341‑3201610

ccTCGTGGGCACGGTTAACGCCGGCGAGGAAGTGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTgc                                                                                                                                    >  1:1142530/1‑140 (MQ=255)

 cTCGTGGGCACGGTTAACGCCGGCGAGGAAGTGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTgcg                                                                                                                                   <  2:1142530/140‑1 (MQ=255)

 cTCGTGGGCACGGTTAACGCCGGCGAGGAAGTGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTgcg                                                                                                                                   >  2:258498/1‑140 (MQ=255)

     tGGGCACGGTTAACGCCGGCGAGGAAGTGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTcc                                                                                                                               <  2:164244/140‑1 (MQ=255)

            ggTTAACGCCGGCGAGGAAGTGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGc                                                                                                                        <  1:928205/140‑1 (MQ=255)

             gTTAACGCCGGCGAGGAAGTGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGcc                                                                                                                       <  2:460587/140‑1 (MQ=255)

             gTTAACGCCGGCGAGGAAGTGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGcc                                                                                                                       <  2:300572/140‑1 (MQ=255)

                 aCGCCGGCGAGGAAGTGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGAt                                                                                                                   >  1:951527/1‑140 (MQ=255)

                      ggCGAGGAAGTGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGa                                                                                                              >  2:373395/1‑140 (MQ=255)

                       gCGAGGAAGTGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGaa                                                                                                             >  1:856020/1‑140 (MQ=255)

                        cGAGGAAGTGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGaaa                                                                                                            >  1:149668/1‑140 (MQ=255)

                            gAAGTGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCa                                                                                                        <  1:801994/140‑1 (MQ=255)

                            gAAGTGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCa                                                                                                        <  2:149668/140‑1 (MQ=255)

                                tGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTc                                                                                                    <  2:16912/140‑1 (MQ=255)

                                 gACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCa                                                                                                   <  2:12507/140‑1 (MQ=255)

                                        ttaCAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCt                                                                                            <  2:951527/140‑1 (MQ=255)

                                          aCAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGa                                                                                          <  1:258498/140‑1 (MQ=255)

                                          aCAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGa                                                                                          <  2:117905/140‑1 (MQ=255)

                                             aaCTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCg                                                                                       >  2:721594/1‑140 (MQ=255)

                                               cTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGAt                                                                                     <  2:311113/140‑1 (MQ=255)

                                                tGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATa                                                                                    >  1:417984/1‑140 (MQ=255)

                                                  aCGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATaaa                                                                                  >  1:871775/1‑140 (MQ=255)

                                                           ccAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGACAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAat                                                                         <  2:621136/140‑1 (MQ=255)

                                                              aTTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATc                                                                      >  2:355935/1‑140 (MQ=255)

                                                                 aTGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGAt                                                                   <  2:29566/140‑1 (MQ=255)

                                                                 aTGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGAt                                                                   <  2:722660/140‑1 (MQ=255)

                                                                 aTGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGAt                                                                   <  2:856020/140‑1 (MQ=255)

                                                                  tGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATa                                                                  <  1:389049/140‑1 (MQ=255)

                                                                  tGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATa                                                                  <  1:233067/140‑1 (MQ=255)

                                                                   gCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATaa                                                                 <  1:467161/140‑1 (MQ=255)

                                                                   gCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATaa                                                                 <  1:947923/140‑1 (MQ=255)

                                                                    cccAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATaa                                                                 <  2:1057101/139‑1 (MQ=255)

                                                                    cccAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATaa                                                                 >  1:1057101/1‑139 (MQ=255)

                                                                    cccAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAac                                                                >  1:337931/1‑140 (MQ=255)

                                                                    cccAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAac                                                                >  1:415150/1‑140 (MQ=255)

                                                                        ggTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCt                                                            >  2:1221574/1‑140 (MQ=255)

                                                                        ggTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCt                                                            >  2:395201/1‑140 (MQ=255)

                                                                         gTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCAc                                                                             <  2:657986/122‑1 (MQ=255)

                                                                         gTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCAc                                                                             >  1:657986/1‑122 (MQ=255)

                                                                         gTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTg                                                           >  2:948424/1‑140 (MQ=255)

                                                                         gTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTg                                                           >  1:402623/1‑140 (MQ=255)

                                                                                  aGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAgcg                                                  <  2:871775/140‑1 (MQ=255)

                                                                                  aGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAgcg                                                  <  2:337931/140‑1 (MQ=255)

                                                                                   gCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGAt                                                                   <  1:328230/122‑1 (MQ=255)

                                                                                   gCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGAt                                                                   >  2:328230/1‑122 (MQ=255)

                                                                                   gCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGCGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAgcgc                                                 >  2:598724/1‑140 (MQ=255)

                                                                                      ctGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAgcg                                                  >  1:577070/1‑136 (MQ=255)

                                                                                      ctGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAgcg                                                  <  2:577070/136‑1 (MQ=255)

                                                                                      ctGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCAcc                                              >  1:298442/1‑140 (MQ=255)

                                                                                       tGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCg                                             >  1:355551/1‑140 (MQ=255)

                                                                                              aCCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAAt                                      >  2:610135/1‑140 (MQ=255)

                                                                                                           cGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATgcagc                                 >  2:154511/1‑132 (MQ=255)

                                                                                                           cGTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATgcagc                                 <  1:154511/132‑1 (MQ=255)

                                                                                                            gTTGAAACAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTgg                        >  1:421257/1‑140 (MQ=255)

                                                                                                                aaaCAACTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCa                    <  1:610135/140‑1 (MQ=255)

                                                                                                                 aacaacTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGgcg                      >  1:414971/1‑137 (MQ=255)

                                                                                                                 aacaacTTAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGgcg                      <  2:414971/137‑1 (MQ=255)

                                                                                                                        tAGCACTGAGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACa            <  1:1015111/140‑1 (MQ=255)

                                                                                                                                aGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCagag                 <  1:795919/127‑1 (MQ=255)

                                                                                                                                aGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCagag                 >  2:795919/1‑127 (MQ=255)

                                                                                                                                aGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATc    >  1:715810/1‑140 (MQ=255)

                                                                                                                                aGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATc    >  1:945945/1‑140 (MQ=255)

                                                                                                                                aGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATc    >  1:996974/1‑140 (MQ=255)

                                                                                                                                aGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATc    >  2:1001270/1‑140 (MQ=255)

                                                                                                                                aGCCAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATc    >  1:1043866/1‑140 (MQ=255)

                                                                                                                                  ccAAGTCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCaa  <  2:402623/140‑1 (MQ=255)

CCTCGTGGGCACGGTTAACGCCGGCGAGGAAGTGACCTTATTACAAACTGACGCCAACACCAATTATGCCCAGGTGAAAGACAGCTCTGGCCGTACCGCCTGGATCCCGTTGAAACAACTTAGCACTGAGCCAAGCCTGCGCTCCCGTGTGCCAGATCTGGAAAATCAGGTCAAAACCCTGACCGATAAACTCACCAATATCGATAACACCTGGAATCAGCGCACCGCAGAAATGCAGCAAAAAGTGGCGCAGAGCGACAGCGTGATCAA  >  NC_000913/3201341‑3201610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A