Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,275,916	G→A	G212D (GGC→GAC)	garD →	galactarate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,275,916	0	G	A	100.0%	549.2 / NA	160	G212D (GGC→GAC)	garD	galactarate dehydratase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (72/88); total (72/88)

Only 100 of 177 total aligned reads displayed.

CGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGAT  >  NC_000913/3275782‑3276052

cGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGGCGTTTTAACTATCCGTACCCTTCACAAAATTTTGCTGGATCCTTACTTTTGCAGCGAAGGAATGGGGATTGACCTgg                                                                                                                                     >  2:308176/1‑140 (MQ=255)

 gATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGGTTAACGCAACGGCGGGAGGTGTACCTATCCGTACCCTTCACAATATTTTGCTGAATCCTTACTTTTGCGGCGGAGGAATGGGGAATTACCTggg                                                                                                                                    >  2:2002224/1‑140 (MQ=255)

 gATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTggg                                                                                                                                    <  1:2049917/140‑1 (MQ=255)

        tggtggGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGaa                                                                                                                             <  2:293751/140‑1 (MQ=255)

          gtggGGCTGAATCATTTGTACGGTTGTGGCGTGGGGGTTAACGCACCGGCGGCAGTTGTACCTATCCGTACCCTTTACAATATTTTGCTGGATCCTAACTTTTGCGGCGGAGTAATGGGGATTGACCTGGGGTGTGgaaa                                                                                                                           >  2:524261/1‑140 (MQ=255)

          gtggGGCTGAATCATTTGTACGGTTGTGGCGTGGCGGTTAAAGCACCGGCGGCCGTTGTACCTATTCGTACCATTTACAATATTTCGCTGAATCCTAACTTTGGGGGCGGAGGAATGGTGATTGACCTGGGGTGTGaaaa                                                                                                                           >  2:1877445/1‑140 (MQ=255)

               gCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGc                                                                                                                      >  2:2204267/1‑140 (MQ=255)

                   aaTCATTTGTACGGTTGTGGCGTGGCGGTTAACGCACCGGCGGCAGGTGTACCTATCCGTACCATTCACAAAATTTTGCTGAATCCTAAATTTTGCGGCGAAGGAATGGTGATTTACCTGGGGTGTGAAAAAGTGCAGcc                                                                                                                  >  2:1154036/1‑140 (MQ=255)

                   aaTCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGcc                                                                                                                  >  2:87565/1‑140 (MQ=255)

                   aaTCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGcc                                                                                                                  <  2:212567/140‑1 (MQ=255)

                   aaTCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAAGATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGcc                                                                                                                  >  2:1674596/1‑140 (MQ=255)

                    aTCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCt                                                                                                                 >  2:740483/1‑140 (MQ=255)

                     tCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTg                                                                                                                <  2:1789522/140‑1 (MQ=255)

                       aTTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAg                                                                                                              <  2:312543/140‑1 (MQ=255)

                        tttGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAgc                                                                                                             <  1:169453/140‑1 (MQ=255)

                        tttGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAgc                                                                                                             <  1:2189282/140‑1 (MQ=255)

                               ggTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCctgct                                                                                                      <  2:822559/140‑1 (MQ=255)

                                  tgtgGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGAc                                                                                                   <  1:1674596/140‑1 (MQ=255)

                                   gtggcgtggcGATTAACGCACCGGCGGGAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGGAGTAATGGTGATTGACCTGGGGTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACt                                                                                                  >  2:955680/1‑140 (MQ=255)

                                   gtggcgtggcGATTAACGCACCGGCGGCAGTTGTACCTTTCCGTACCATTCACAATATTTTGCCGAAACCTTAATTTGGCCGCGGAGGAATGGTGATTGGCCTTGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACt                                                                                                  >  2:2049363/1‑140 (MQ=255)

                                   gtggcgtggcGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACt                                                                                                  <  1:2002224/140‑1 (MQ=255)

                                      gcgtggcgATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGa                                                                                               >  2:1776322/1‑140 (MQ=255)

                                       cgtggcgATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGaa                                                                                              <  1:1636468/140‑1 (MQ=255)

                                        gtggcgATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAAc                                                                                             <  1:239338/140‑1 (MQ=255)

                                          ggcgATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGGTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACgg                                                                                           >  1:1438325/1‑140 (MQ=255)

                                           gcgATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGga                                                                                          >  2:1258279/1‑140 (MQ=255)

                                           gcgATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCACTGAATCCTAACTTTTGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGga                                                                                          >  2:488542/1‑140 (MQ=255)

                                             gATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGgatg                                                                                        <  2:1296864/140‑1 (MQ=255)

                                             gATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGgatg                                                                                        <  1:1153628/140‑1 (MQ=255)

                                             gATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGgatg                                                                                        <  1:1776322/140‑1 (MQ=255)

                                             gATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGgatg                                                                                        <  1:2204267/140‑1 (MQ=255)

                                                  aCGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGAtgtg                                                                                   <  1:1530526/140‑1 (MQ=255)

                                                   cGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGc                                                                                  >  1:140884/1‑140 (MQ=255)

                                                    gCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCa                                                                                 <  2:1539363/140‑1 (MQ=255)

                                                    gCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCa                                                                                 <  1:2278182/140‑1 (MQ=255)

                                                     cACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGAtgtg                                                                                   >  2:415774/1‑137 (MQ=255)

                                                     cACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGAtgtg                                                                                   <  1:415774/137‑1 (MQ=255)

                                                      aCCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAg                                                                               <  1:1715346/140‑1 (MQ=255)

                                                       ccGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGc                                                                              <  1:1497877/140‑1 (MQ=255)

                                                       ccGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGc                                                                              <  1:1305538/140‑1 (MQ=255)

                                                         ggcggcAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTa                                                                            <  1:1073689/140‑1 (MQ=255)

                                                             gcAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTcc                                                                        <  1:740483/140‑1 (MQ=255)

                                                                        tATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAgcg                                                             >  1:1928128/1‑140 (MQ=255)

                                                                          tCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGcc                                                           <  1:2406841/140‑1 (MQ=255)

                                                                          tCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGcc                                                           >  2:1727134/1‑140 (MQ=255)

                                                                           ccGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCa                                                          >  1:1883804/1‑140 (MQ=255)

                                                                           ccGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCa                                                          >  1:60652/1‑140 (MQ=255)

                                                                              tACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGcc                                                           >  1:1747816/1‑136 (MQ=255)

                                                                              tACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGcc                                                           <  2:1747816/136‑1 (MQ=255)

                                                                              tACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCa                                                       >  2:2310880/1‑140 (MQ=255)

                                                                              tACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCa                                                       >  2:888779/1‑140 (MQ=255)

                                                                                ccATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCAtt                                                     <  1:1258279/140‑1 (MQ=255)

                                                                                   ttCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAg                                                         <  2:1521617/133‑1 (MQ=255)

                                                                                   ttCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTc                                                  >  1:1957947/1‑140 (MQ=255)

                                                                                   ttCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTc                                                  >  1:1941307/1‑140 (MQ=255)

                                                                                   ttCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTACAAGCTATTCCAGTAGAAAGCGCCAGCATTGTc                                                  >  1:1000503/1‑140 (MQ=255)

                                                                                    tCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGcc                                                           <  1:1028163/130‑1 (MQ=255)

                                                                                    tCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGcc                                                           >  2:1058629/1‑130 (MQ=255)

                                                                                    tCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGcc                                                           <  1:1058629/130‑1 (MQ=255)

                                                                                    tCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCAt                                                      <  1:2297570/135‑1 (MQ=255)

                                                                                     cacaATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGcc                                                           <  2:1127605/129‑1 (MQ=255)

                                                                                     cacaATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAg                                                >  1:389217/1‑140 (MQ=255)

                                                                                        aaTATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGttt                                             >  2:118420/1‑140 (MQ=255)

                                                                                        aaTATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGttt                                             >  2:1834286/1‑140 (MQ=255)

                                                                                        aaTATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGttt                                             >  2:1037497/1‑140 (MQ=255)

                                                                                          tatTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGc                                           <  1:1485465/140‑1 (MQ=255)

                                                                                          tatTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGc                                           <  2:1583086/140‑1 (MQ=255)

                                                                                           atTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCa                                          >  2:1025706/1‑140 (MQ=255)

                                                                                           atTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCa                                          <  2:440027/140‑1 (MQ=255)

                                                                                           atTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCa                                          >  2:1055463/1‑140 (MQ=255)

                                                                                           atTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCa                                          >  2:2241022/1‑140 (MQ=255)

                                                                                            tttCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAg                                         >  1:2235723/1‑140 (MQ=255)

                                                                                               cGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGa                                       <  1:2061401/139‑1 (MQ=255)

                                                                                               cGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGa                                       >  2:2061401/1‑139 (MQ=255)

                                                                                               cGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGAt                                      >  2:214827/1‑140 (MQ=255)

                                                                                                 cTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGa                                    <  1:1814250/140‑1 (MQ=255)

                                                                                                  tGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGaa                                   >  2:1452235/1‑140 (MQ=255)

                                                                                                    aaTCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGaaaa                                 >  1:698817/1‑140 (MQ=255)

                                                                                                    aaTCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGaaaa                                 >  1:2172517/1‑140 (MQ=255)

                                                                                                    aaTCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTAGTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGaaaa                                 >  2:2246945/1‑140 (MQ=255)

                                                                                                      tCCTAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGc                               >  2:1086126/1‑140 (MQ=255)

                                                                                                         tAACTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATg                            >  1:813560/1‑140 (MQ=255)

                                                                                                          aaCTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGt                           <  1:1542529/140‑1 (MQ=255)

                                                                                                           aCTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTc                          <  1:87671/140‑1 (MQ=255)

                                                                                                            cTTTGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCg                         <  2:1883804/140‑1 (MQ=255)

                                                                                                              ttGGCGGCGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGt                       <  1:1086126/140‑1 (MQ=255)

                                                                                                                ggcggcGAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGttt                     >  1:2317388/1‑140 (MQ=255)

                                                                                                                 gcggcgAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGtttt                    <  1:118420/140‑1 (MQ=255)

                                                                                                                 gcggcgAAGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGtttt                    <  1:488542/140‑1 (MQ=255)

                                                                                                                        aGTAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCa             <  1:432476/140‑1 (MQ=255)

                                                                                                                          tAATGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATg           <  2:813560/140‑1 (MQ=255)

                                                                                                                            aTGGTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGt         >  1:1358209/1‑140 (MQ=255)

                                                                                                                               gTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGa      <  1:1055463/140‑1 (MQ=255)

                                                                                                                               gTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGa      <  1:1834286/140‑1 (MQ=255)

                                                                                                                               gTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGa      <  1:2122462/140‑1 (MQ=255)

                                                                                                                               gTGATTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGa      <  2:1957947/140‑1 (MQ=255)

                                                                                                                                  aTTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGa   >  2:514685/1‑140 (MQ=255)

                                                                                                                                  aTTGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGa   >  2:1125552/1‑140 (MQ=255)

                                                                                                                                   ttGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAgg    >  2:1369985/1‑138 (MQ=255)

                                                                                                                                   ttGACCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGat  >  2:627890/1‑140 (MQ=255)

CGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGAT  >  NC_000913/3275782‑3276052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A