Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,931,183	(G)_7→8	intergenic (‑90/‑133)	ravA ← / → kup	regulatory ATPase RavA/K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,931,176	1	.	G	97.1%	475.6 / 5.1	137	intergenic (‑83/‑140)	ravA/kup	regulatory ATPase RavA/K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand): ref base . (2/2); new base G (62/71); total (64/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01

Only 100 of 148 total aligned reads displayed.

TTCCAACGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGACT  >  NC_000913/3931037‑3931310

ttCCAACGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAATTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCaaa                                                                                                                                          <  2:1840172/140‑1 (MQ=255)

   cAACGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑‑ggg                                                                                                                                     <  2:1555511/140‑1 (MQ=255)

     aCGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑‑ggggg                                                                                                                                   <  2:2365636/140‑1 (MQ=255)

       gAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑‑ggggggg                                                                                                                                 <  2:2073000/140‑1 (MQ=255)

          cTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑‑ggggggggta                                                                                                                              >  1:894800/1‑137 (MQ=255)

             cTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTAttt                                                                                                                            >  1:1340925/1‑140 (MQ=255)

                aGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTc                                                                                                                         >  1:2285088/1‑140 (MQ=255)

                 ggCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCt                                                                                                                        <  1:1268337/140‑1 (MQ=255)

                  gCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCtt                                                                                                                       <  1:1985598/140‑1 (MQ=255)

                  gCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCtt                                                                                                                       >  2:224034/1‑140 (MQ=255)

                     ggAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑‑ggggggg                                                                                                                                 >  2:1613448/1‑126 (MQ=255)

                     ggAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑‑ggggggg                                                                                                                                 <  1:1613448/126‑1 (MQ=255)

                      gAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAg                                                                                                                   <  2:2023100/140‑1 (MQ=255)

                      gAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAg                                                                                                                   <  1:848856/140‑1 (MQ=255)

                      gAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAg                                                                                                                   <  2:46738/140‑1 (MQ=255)

                       aaaTTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTGAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGc                                                                                                                  >  2:242380/1‑140 (MQ=255)

                       aaaTTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGc                                                                                                                  >  1:585929/1‑140 (MQ=255)

                        aaTTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGcc                                                                                                                 <  1:541220/140‑1 (MQ=255)

                            cTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGt                                                                                                             >  1:167058/1‑140 (MQ=255)

                            cTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGt                                                                                                             >  2:227232/1‑140 (MQ=255)

                             tttCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGtt                                                                                                            >  1:1798885/1‑140 (MQ=255)

                                    taataaATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTgg                                                                                                     <  2:812570/140‑1 (MQ=255)

                                    taataaATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTgg                                                                                                     <  2:1392556/140‑1 (MQ=255)

                                     aataaATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCttt                                                                                                                      <  1:1336841/122‑1 (MQ=255)

                                         aaTGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCa                                                                                                <  2:918691/140‑1 (MQ=255)

                                              gggTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGcc                                                                                                   <  2:1044896/132‑1 (MQ=255)

                                              gggTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGcc                                                                                                   >  1:1044896/1‑132 (MQ=255)

                                              gggTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAg                                                                                           >  1:2192550/1‑140 (MQ=255)

                                              gggTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAg                                                                                           <  2:875158/140‑1 (MQ=255)

                                              gggTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAg                                                                                           <  1:348634/140‑1 (MQ=255)

                                                gTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTa                                                                                            >  1:1965746/1‑137 (MQ=255)

                                                gTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTa                                                                                            <  2:1965746/137‑1 (MQ=255)

                                                gTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGa                                                                                         >  2:1064315/1‑140 (MQ=255)

                                                gTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGa                                                                                         >  1:174905/1‑140 (MQ=255)

                                                 tGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAg                                                                                        <  2:2031155/140‑1 (MQ=255)

                                                   aGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTa                                                                                      >  1:358331/1‑140 (MQ=255)

                                                   aGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTa                                                                                      >  2:852601/1‑140 (MQ=255)

                                                   aGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTa                                                                                      >  2:177235/1‑140 (MQ=255)

                                                   aGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTa                                                                                      >  1:2181628/1‑140 (MQ=255)

                                                        tAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGAtt                                                                                 >  2:1889247/1‑140 (MQ=255)

                                                         aaTGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAGGGGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGAtt                                                                                 <  1:874304/140‑1 (MQ=255)

                                                              aGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAGGGGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGcc                                                                            >  1:1371326/1‑140 (MQ=255)

                                                               gTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGt                                                                          >  1:385000/1‑140 (MQ=255)

                                                               gTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGt                                                                          <  2:894800/140‑1 (MQ=255)

                                                               gTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGt                                                                          <  2:680417/140‑1 (MQ=255)

                                                               gTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGt                                                                          <  1:1887216/140‑1 (MQ=255)

                                                               gTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGt                                                                          <  1:1889247/140‑1 (MQ=255)

                                                               gTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGt                                                                          >  1:2021902/1‑140 (MQ=255)

                                                               gTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGt                                                                          >  2:1086835/1‑140 (MQ=255)

                                                               gTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGt                                                                          <  1:30145/140‑1 (MQ=255)

                                                                tGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAGGGGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGt                                                                          <  2:1371326/140‑1 (MQ=255)

                                                                tGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTa                                                                        <  1:41400/140‑1 (MQ=255)

                                                                 ggCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGttt                                                                        <  1:852601/140‑2 (MQ=255)

                                                                 ggCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTa                                                                        <  1:2185070/140‑1 (MQ=255)

                                                                 ggCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTa                                                                        <  1:1064315/140‑1 (MQ=255)

                                                                 ggCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTa                                                                        <  2:585929/140‑1 (MQ=255)

                                                                 ggCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTa                                                                        <  2:2285088/140‑1 (MQ=255)

                                                                 ggCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTa                                                                        <  2:1822607/140‑1 (MQ=255)

                                                                 ggCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTa                                                                        <  1:227232/140‑1 (MQ=255)

                                                                  gCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGTGTACGAtt                                                                                 <  2:1575041/130‑1 (MQ=255)

                                                                  gCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGTGTACGAtt                                                                                 >  1:1575041/1‑130 (MQ=255)

                                                                  gCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTaa                                                                       >  2:1137724/1‑140 (MQ=255)

                                                                  gCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTaa                                                                       >  2:381581/1‑140 (MQ=255)

                                                                   cGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAAt                                                                      <  1:1086835/140‑1 (MQ=255)

                                                                   cGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAAt                                                                      <  2:1340925/140‑1 (MQ=255)

                                                                   cGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAAt                                                                      <  2:792592/140‑1 (MQ=255)

                                                                     tCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCg                                                                    >  2:845715/1‑140 (MQ=255)

                                                                      ccTTTCGTCAAAAGTTCTGCATAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGt                                                                   >  2:913939/1‑140 (MQ=255)

                                                                       cTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGGATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTg                                                                  >  1:759455/1‑140 (MQ=255)

                                                                          tCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGAtttt                                                                               <  2:228991/124‑1 (MQ=255)

                                                                          tCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGAtttt                                                                               >  1:228991/1‑124 (MQ=255)

                                                                               aaaaGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACtgt                                                          <  1:768144/140‑1 (MQ=255)

                                                                                       tGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCtttt                                                  >  1:1171100/1‑140 (MQ=255)

                                                                                       tGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCtttt                                                  >  2:1021864/1‑140 (MQ=255)

                                                                                        gCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCttttt                                                 >  1:475184/1‑140 (MQ=255)

                                                                                         cGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCtttttt                                                >  2:615734/1‑140 (MQ=255)

                                                                                          gTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTtg                                               <  2:1729446/140‑1 (MQ=255)

                                                                                            aaaTTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTtgtg                                             <  1:2230853/140‑1 (MQ=255)

                                                                                                 gCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCa                                        <  2:2186516/140‑1 (MQ=255)

                                                                                                 gCGAGTATAGACGTTTCTTGCAGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATATTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCa                                        <  2:2192550/140‑1 (MQ=255)

                                                                                                   gAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCTTTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAg                                      >  2:2110857/1‑140 (MQ=255)

                                                                                                     gTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAggg                                    <  2:1357897/140‑1 (MQ=255)

                                                                                                      tataGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGa                                   >  2:1198947/1‑140 (MQ=255)

                                                                                                             gTTTCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGca                            <  2:1092145/140‑1 (MQ=255)

                                                                                                               ttCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGcaca                          <  1:2110857/140‑1 (MQ=255)

                                                                                                               ttCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGcaca                          <  1:607717/140‑1 (MQ=255)

                                                                                                               ttCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGcaca                          >  2:1589496/1‑140 (MQ=255)

                                                                                                                tCTTGCTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGcacac                         >  1:1573132/1‑140 (MQ=255)

                                                                                                                     cTGGTGGCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTc                    >  2:1273505/1‑140 (MQ=255)

                                                                                                                       ggtggCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGc                             >  1:1234533/1‑129 (MQ=255)

                                                                                                                       ggtggCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGc                             <  2:1234533/129‑1 (MQ=255)

                                                                                                                        gtggCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCata                 >  2:2174780/1‑140 (MQ=255)

                                                                                                                         tggCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCatat                <  1:1198684/140‑1 (MQ=255)

                                                                                                                           gCTAAAATAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATAttt              <  2:2002073/140‑1 (MQ=255)

                                                                                                                                  tAGTCTCAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGaa       <  2:1887379/140‑1 (MQ=255)

                                                                                                                                     tctcAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGa    >  1:1469593/1‑140 (MQ=255)

                                                                                                                                     tctcAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGa    <  1:913939/140‑1 (MQ=255)

                                                                                                                                     tctcAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGa    >  2:321155/1‑140 (MQ=255)

                                                                                                                                       tcAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGACt  <  1:321155/140‑1 (MQ=255)

                                                                                                                                       tcAAAG‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGACt  <  1:915597/140‑1 (MQ=255)

TTCCAACGAACTGCTCAGGCGGGAAATTCTTTCCGCTAATAAATGAGGGTGAGCCATAATGAAGTGGCGTCCTTTCGTCAAAAGTTCTGCGTAAATTGCGAGTATAGACGTTTCTTGCTGGTGGCTAAAATAGTCTCAAA‑‑GGGGGGGTATTTTTCTTTGAGCCAGGTTAATGTGGCCGCATTTAGGAGTACGATTTTGCCGTTAATCGTGCATACTGTGCGCTTTTTTGTGGGCCAAGGGACTAAGCACACATTTCATATTTCAACGAAAGACT  >  NC_000913/3931037‑3931310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A