Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 180 66.5 2263572 99.4% 2249990 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,934,196 G→A G140D (GGC→GAC)  rbsA → ribose ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,934,1960GA100.0% 167.2 / NA 48G140D (GGC→GAC) rbsAribose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/24);  total (24/24)

ACCATTGCCGAAAACATTTTCCTCGGTCGTGAGTTTGTTAATCGCTTTGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGGCGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAATCCCTGTTCCGC  >  NC_000913/3934060‑3934323
                                                                                                                                        |                                                                                                                               
aCCATTGCCGAAAACATTTTCCTCGGTCGTGAGTTTGTTAATCGCTTTGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGa                                                                                                                              >  2:214441/1‑140 (MQ=255)
 ccATTGCCGAAAACATTTTCCTCGGTCGTGAGTTTGTTAATCGCTTTGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGAt                                                                                                                             <  2:863788/140‑1 (MQ=255)
   aTTGCCGAAAACATTTTCCTCGGTCGTGAGTTTGTTAATCGCTTTGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCt                                                                                                                           <  1:371258/140‑1 (MQ=255)
     tGCCGAAAACATTTTCCTCGGTCGTGAGTTTGTTAATCGCTTTGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCttt                                                                                                                         >  1:614136/1‑140 (MQ=255)
      gCCGAAAACATTTTCCTCGGTCGTGAGTTTGTTAATCGCTTTGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTc                                                                                                                        <  1:370819/140‑1 (MQ=255)
              cATTTTCCTCGGTCGTGAGTTTGTTAATCGCTTTGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTg                                                                                                                >  1:948993/1‑140 (MQ=255)
                    ccTCGGTCGTGAGTTTGTTAATCGCTTTGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGc                                                                                                          >  1:745711/1‑140 (MQ=255)
                      tCGGTCGTGAGTTTGTTAATCGCTTTGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCaa                                                                                                        <  1:61365/140‑1 (MQ=255)
                             tGAGTTTGTTAATCGCTTTGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTg                                                                                                 >  2:1053659/1‑140 (MQ=255)
                                    gTTAATCGCTTTGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGc                                                                                          >  2:504316/1‑140 (MQ=255)
                                      tAATCGCTTTGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCa                                                                                        <  2:745711/140‑1 (MQ=255)
                                        aTCGCTTTGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGATAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGCCGACCAGCAAATGGTTGAAATCGCCaaa                                                                                      >  1:427485/1‑140 (MQ=255)
                                          cGCTTTGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGt                                                                                    >  1:706397/1‑140 (MQ=255)
                                               tGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCATAGTg                                                                                   <  2:9081/136‑1 (MQ=255)
                                               tGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTg                                                                                   >  1:9081/1‑136 (MQ=255)
                                                    aaaTTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCttt                                                                          <  2:942720/140‑1 (MQ=255)
                                                                aaaCCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGtc                                                              <  2:528426/140‑1 (MQ=255)
                                                                 aaCCATGTATACCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGtca                                                             <  1:1053659/140‑1 (MQ=255)
                                                                  aCCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGtcat                                                            <  2:706397/140‑1 (MQ=255)
                                                                     aTGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTcatcat                                                         <  2:614136/140‑1 (MQ=255)
                                                                     aTGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTcatcat                                                         <  2:1067174/140‑1 (MQ=255)
                                                                     aTGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATAGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTcatcat                                                         <  2:852088/140‑1 (MQ=255)
                                                                         aTGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATg                                                     >  1:256324/1‑140 (MQ=255)
                                                                            ccGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTACGGAt                                                  <  2:809403/140‑1 (MQ=255)
                                                                             cGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATg                                                 >  2:128553/1‑140 (MQ=255)
                                                                              gAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGCTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGa                                                >  1:102796/1‑140 (MQ=255)
                                                                                aGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAac                                              >  1:1008658/1‑140 (MQ=255)
                                                                                    gATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAaccgac                                          >  2:512451/1‑140 (MQ=255)
                                                                                     aTAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAaccgacc                                         <  2:601612/140‑1 (MQ=255)
                                                                                      tAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAaccgaccg                                        <  1:803351/140‑1 (MQ=255)
                                                                                           tGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATgcg                                   <  1:504316/140‑1 (MQ=255)
                                                                                                         aaCCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATaccga                     <  2:743654/140‑1 (MQ=255)
                                                                                                          aCCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGAcc                             <  2:848934/131‑1 (MQ=255)
                                                                                                          aCCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGAcc                             >  1:848934/1‑131 (MQ=255)
                                                                                                          aCCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATaccgaa                    >  2:1096541/1‑140 (MQ=255)
                                                                                                          aCCTGCGCTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATaccgaa                    >  2:721073/1‑140 (MQ=255)
                                                                                                              gcgcTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGGAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATaccgaaaccg                <  2:948993/140‑1 (MQ=255)
                                                                                                               cgcTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATaccgaaaccga               >  2:588473/1‑140 (MQ=255)
                                                                                                               cgcTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATaccgaaaccga               >  1:879262/1‑140 (MQ=255)
                                                                                                               cgcTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATaccgaaaccga               >  1:910151/1‑140 (MQ=255)
                                                                                                               cgcTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATaccgaaaccga               >  2:195251/1‑140 (MQ=255)
                                                                                                                 cTTTAAAAGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAAt             <  1:195251/140‑1 (MQ=255)
                                                                                                                     aaaaGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGACTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAATCCCt         <  2:1008658/140‑1 (MQ=255)
                                                                                                                       aaGCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAATCCCTGt       <  1:128553/140‑1 (MQ=255)
                                                                                                                         gCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAATCCCTGtt      <  1:58799/139‑1 (MQ=255)
                                                                                                                         gCGACAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAATCCCTGtt      >  2:58799/1‑139 (MQ=255)
                                                                                                                            aCAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAATCCCTGTTCcgc  >  1:662310/1‑140 (MQ=255)
                                                                                                                            aCAAGCTGGTGGACGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAATCCCTGTTCcgc  >  1:71128/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                               
ACCATTGCCGAAAACATTTTCCTCGGTCGTGAGTTTGTTAATCGCTTTGGCAAAATTGACTGGAAAACCATGTATGCCGAAGCGGATAAATTGCTGGCTAAACTTAACCTGCGCTTTAAAAGCGACAAGCTGGTGGGCGATCTTTCCATCGGTGACCAGCAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAATCCCTGTTCCGC  >  NC_000913/3934060‑3934323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A