Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 258 142.3 4824480 99.1% 4781059 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,235,842 A→G intergenic (+435/‑64) pgi → / → yjbE glucose‑6‑phosphate isomerase/uncharacterized protein YjbE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,235,8420AG99.2% 482.8 / ‑3.7 134intergenic (+435/‑64)pgi/yjbEglucose‑6‑phosphate isomerase/uncharacterized protein YjbE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (65/68);  minor base . (1/0);  total (66/68)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.68e-01

Only 100 of 135 total aligned reads displayed.

TTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCAC  >  NC_000913/4235707‑4235972
                                                                                                                                       |                                                                                                                                  
tttGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACt                                                                                                                                >  2:1696028/1‑140 (MQ=255)
tttGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACt                                                                                                                                >  2:46331/1‑140 (MQ=255)
tttGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACt                                                                                                                                >  1:1219652/1‑140 (MQ=255)
       ttattttaAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATccc                                                                                                                         <  2:899113/140‑1 (MQ=255)
        tattttaAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATcccg                                                                                                                        >  2:692077/1‑140 (MQ=255)
            ttaAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATcccgcccg                                                                                                                    <  2:1286307/140‑1 (MQ=255)
                    aTGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGttt                                                                                                            >  1:1798063/1‑140 (MQ=255)
                    aTGATATTTATTGTTATATAAATATAAGTTGAAACTTATATTTGATATTCATCCCAATTATCCTAAAACGCCACCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCAATCCCGCCCGACATGttt                                                                                                            <  2:1637482/140‑1 (MQ=255)
                          tttATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGt                                                                                                      <  1:692077/140‑1 (MQ=255)
                           ttATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTa                                                                                                     <  2:1540578/140‑1 (MQ=255)
                           ttATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTa                                                                                                     >  1:1775533/1‑140 (MQ=255)
                           ttATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTa                                                                                                     >  1:626874/1‑140 (MQ=255)
                             aTTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTaaa                                                                                                   <  1:1218923/140‑1 (MQ=255)
                              ttGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAAt                                                                                                  <  2:1219652/140‑1 (MQ=255)
                                   aTTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTaaa                                                                                                   >  2:208903/1‑134 (MQ=255)
                                   aTTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTaaa                                                                                                   <  1:560366/134‑1 (MQ=255)
                                   aTTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTaaa                                                                                                   >  2:560366/1‑134 (MQ=255)
                                   aTTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCca                                                                                             >  1:1768452/1‑140 (MQ=255)
                                      tAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCcat                                                                                            <  1:1156022/138‑1 (MQ=255)
                                      tAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCcat                                                                                            >  2:1156022/1‑138 (MQ=255)
                                        aaTATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCT‑AAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCcattcatt                                                                                       <  2:2276186/140‑1 (MQ=255)
                                         atatAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCcattcatt                                                                                       <  1:1605098/140‑1 (MQ=255)
                                           ataAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCAttcattca                                                                                     <  2:1775533/140‑1 (MQ=255)
                                           ataAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCAttcattca                                                                                     >  2:1624519/1‑140 (MQ=255)
                                           ataAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCAttcattca                                                                                     <  1:46331/140‑1 (MQ=255)
                                              aGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATg                                                                                  <  2:101673/140‑1 (MQ=255)
                                                ttGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGaa                                                                                <  2:1155147/140‑1 (MQ=255)
                                                ttGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGaa                                                                                <  2:1653565/140‑1 (MQ=255)
                                                  gAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAgg                                                                              >  1:1359154/1‑140 (MQ=255)
                                                        tataTTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAAtt                                                                        >  1:1675068/1‑140 (MQ=255)
                                                         atatTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGaaa                                                                          <  1:1395820/137‑1 (MQ=255)
                                                          tatTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAg                                                                               >  1:2038387/1‑131 (MQ=255)
                                                          tatTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAg                                                                               <  2:2038387/131‑1 (MQ=255)
                                                          tatTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAg                                                                               <  2:421900/131‑1 (MQ=255)
                                                          tatTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAg                                                                               >  1:421900/1‑131 (MQ=255)
                                                          tatTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGt                                                                      >  2:1394989/1‑140 (MQ=255)
                                                          tatTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGt                                                                      >  1:1132764/1‑140 (MQ=255)
                                                          tatTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGt                                                                      >  2:2275200/1‑140 (MQ=255)
                                                             ttGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTAt                                                                   <  2:1675068/140‑1 (MQ=255)
                                                               gATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGtgatga                                                                 >  2:2215036/1‑140 (MQ=255)
                                                               gATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGa                                                                 >  2:1887908/1‑140 (MQ=255)
                                                               gATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGa                                                                 >  1:2168619/1‑140 (MQ=255)
                                                                atatTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGaa                                                                <  2:226914/140‑1 (MQ=255)
                                                                atatTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGaa                                                                >  2:1757080/1‑140 (MQ=255)
                                                                atatTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGaa                                                                >  2:1457396/1‑140 (MQ=255)
                                                                 tatTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGaaa                                                               >  2:941387/1‑140 (MQ=255)
                                                                 tatTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGaaa                                                               >  2:2124806/1‑140 (MQ=255)
                                                                 tatTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGaaa                                                               >  1:1720902/1‑140 (MQ=255)
                                                                 tatTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGaaa                                                               >  2:578319/1‑140 (MQ=255)
                                                                  attcattcCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGaaaa                                                              <  1:592276/140‑1 (MQ=255)
                                                                    tcattcCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGGAATTGTTATGaaaaaa                                                            >  2:2160335/1‑140 (MQ=255)
                                                                    tcattcCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGaaaaaa                                                            >  2:150547/1‑140 (MQ=255)
                                                                    tcattcCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGaaaaaa                                                            >  1:1494493/1‑140 (MQ=255)
                                                                     cattcCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGATTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAg                                                           <  1:1571435/140‑1 (MQ=255)
                                                                      attcCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGt                                                          >  2:1906342/1‑140 (MQ=255)
                                                                       ttcCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGtt                                                         <  2:1359154/140‑1 (MQ=255)
                                                                       ttcCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGtt                                                         <  1:2270734/140‑1 (MQ=255)
                                                                       ttcCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGtt                                                         <  1:1624519/140‑1 (MQ=255)
                                                                       ttcCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGtt                                                         <  2:377061/140‑1 (MQ=255)
                                                                       ttcCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGtt                                                         <  1:941387/140‑1 (MQ=255)
                                                                        tcCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTc                                                        >  2:855404/1‑140 (MQ=255)
                                                                         ccAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCt                                                       <  2:1798063/140‑1 (MQ=255)
                                                                            aTTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTa                                                    <  1:1049102/140‑1 (MQ=255)
                                                                              tATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATg                                                  <  1:1906342/140‑1 (MQ=255)
                                                                                tCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGc                                                >  1:2182647/1‑140 (MQ=255)
                                                                                tCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGc                                                >  1:2252825/1‑140 (MQ=255)
                                                                                 ccTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCa                                               <  2:1720902/140‑1 (MQ=255)
                                                                                  cTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCAt                                              >  2:2285155/1‑140 (MQ=255)
                                                                                  cTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCAt                                              <  2:2012527/140‑1 (MQ=255)
                                                                                   tAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCAtt                                             <  1:1701881/140‑1 (MQ=255)
                                                                                     aaaCGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCAtttt                                           <  1:2127364/140‑1 (MQ=255)
                                                                                         gCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGcc                                       <  1:1394989/140‑1 (MQ=255)
                                                                                         gCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGcc                                       <  2:2252825/140‑1 (MQ=255)
                                                                                           cATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAATGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGc                                        >  2:2230348/1‑137 (MQ=255)
                                                                                           cATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGc                                        <  1:2230348/137‑1 (MQ=255)
                                                                                           cATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGc                                        >  2:39167/1‑137 (MQ=255)
                                                                                           cATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGc                                        <  1:39167/137‑1 (MQ=255)
                                                                                           cATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCat                                     >  1:667661/1‑140 (MQ=255)
                                                                                             tCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCatat                                   >  1:2060962/1‑140 (MQ=255)
                                                                                              cGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATc                                  <  1:1457396/140‑1 (MQ=255)
                                                                                                         cgcgCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGcc                                       <  2:1455451/124‑1 (MQ=255)
                                                                                                         cgcgCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTgcgg                       >  1:295031/1‑140 (MQ=255)
                                                                                                         cgcgCCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTgcgg                       <  1:2215036/140‑1 (MQ=255)
                                                                                                          gcgcCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTgcggc                      <  2:2080033/140‑1 (MQ=255)
                                                                                                           cgcCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCGGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTgcggcg                     >  2:2145492/1‑140 (MQ=255)
                                                                                                           cgcCGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTgcggcg                     >  1:1241618/1‑140 (MQ=255)
                                                                                                             ccGTAATTCGCATGCTTTAGTTGTG‑‑TACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACtt                 >  1:1690228/1‑140 (MQ=255)
                                                                                                              cGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACt                  <  1:2316976/140‑1 (MQ=255)
                                                                                                              cGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACt                  >  1:1675016/1‑140 (MQ=255)
                                                                                                              cGTAATTCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACt                  <  2:641170/140‑1 (MQ=255)
                                                                                                                    tCGCATGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCg            <  1:578319/140‑1 (MQ=255)
                                                                                                                        aTGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTggg        >  1:521757/1‑140 (MQ=255)
                                                                                                                         tGCTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGc       <  1:2145492/140‑1 (MQ=255)
                                                                                                                           cTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTc                <  1:2191977/129‑1 (MQ=255)
                                                                                                                           cTTTAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTc                >  2:2191977/1‑129 (MQ=255)
                                                                                                                            tttAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGc    <  2:2192258/140‑1 (MQ=255)
                                                                                                                            tttAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGc    >  2:1394158/1‑140 (MQ=255)
                                                                                                                            tttAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGc    <  1:2124806/140‑1 (MQ=255)
                                                                                                                              tAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCAc  <  2:1241618/140‑1 (MQ=255)
                                                                                                                              tAGTTGTGTGTACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCAc  <  1:150547/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                  
TTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGGAAATTGTTATGAAAAAAGTTCTGTATGGCATTTTTGCCATATCTGCGCTTGCGGCGACTTCTGCGTGGGCTGCAC  >  NC_000913/4235707‑4235972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A