Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,242,594	T→C	intergenic (‑340/+32)	xylE ← / ← malG	D‑xylose:H(+) symporter/maltose ABC transporter membrane subunit MalG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,242,594	0	T	C	100.0%	120.5 / NA	35	intergenic (‑340/+32)	xylE/malG	D‑xylose:H(+) symporter/maltose ABC transporter membrane subunit MalG
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/16); total (19/16)

AATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAA  >  NC_000913/4242472‑4242729

aataatAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTg                                                                                                                        <  2:192104/140‑1 (MQ=255)

 ataataAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTgg                                                                                                                       <  1:821448/140‑1 (MQ=255)

      aaGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGaa                                                                                                                  >  2:789441/1‑140 (MQ=255)

        gCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGaaca                                                                                                                <  1:61307/140‑1 (MQ=255)

            tATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACAt                                                                                                            >  1:325226/1‑140 (MQ=255)

                atgGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCttt                                                                                                        <  1:1048764/140‑1 (MQ=255)

                   gAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAAc                                                                                                     >  1:672958/1‑140 (MQ=255)

                        atTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTc                                                                                                >  1:709806/1‑140 (MQ=255)

                        atTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTc                                                                                                >  1:1097046/1‑140 (MQ=255)

                            aCCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCacac                                                                                            <  2:331761/140‑1 (MQ=255)

                                gCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACaccacc                                                                                        >  1:100551/1‑140 (MQ=255)

                                gCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACaccacc                                                                                        >  1:98589/1‑140 (MQ=255)

                                 cGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCacac                                                                                            <  1:561347/135‑1 (MQ=255)

                                 cGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCacac                                                                                            >  2:561347/1‑135 (MQ=255)

                                   aaCGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGc                                                                                     <  2:223154/140‑1 (MQ=255)

                                       aTTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTc                                                                                 <  2:66190/140‑1 (MQ=255)

                                            gATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGcc                                                                            <  2:100551/140‑1 (MQ=255)

                                            gATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGcc                                                                            <  2:98589/140‑1 (MQ=255)

                                                  aGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGTAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGAc                                                                      >  1:140214/1‑140 (MQ=255)

                                                  aGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGAc                                                                      >  1:13958/1‑140 (MQ=255)

                                                                    cgcTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAg                                                    >  1:66614/1‑140 (MQ=255)

                                                                          gTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCaagaa                                              <  1:789441/140‑1 (MQ=255)

                                                                                   cGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGa                                         >  1:444003/1‑136 (MQ=255)

                                                                                   cGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGa                                         <  2:444003/136‑1 (MQ=255)

                                                                                    gAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTg                                    <  2:13958/140‑1 (MQ=255)

                                                                                     aaTAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGa                                   >  1:694899/1‑140 (MQ=255)

                                                                                      aTAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGAt                                  <  2:709806/140‑1 (MQ=255)

                                                                                           aGTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTa                             <  1:972944/140‑1 (MQ=255)

                                                                                            gTCACAAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTaa                            >  2:802401/1‑140 (MQ=255)

                                                                                               acaAGTTGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGc                         >  1:684874/1‑140 (MQ=255)

                                                                                                     tGGGATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACAt                   >  2:1126071/1‑140 (MQ=255)

                                                                                                        gATAACGTAAGTTGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCAc                >  1:633807/1‑140 (MQ=255)

                                                                                                                    tGAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCggcggc    <  1:147571/140‑1 (MQ=255)

                                                                                                                     gAGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCa   >  2:123399/1‑140 (MQ=255)

                                                                                                                      aGGGCGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCaa  >  2:805143/1‑140 (MQ=255)

AATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAA  >  NC_000913/4242472‑4242729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A