Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,442,353	+G	intergenic (‑177/‑29)	msrA ← / → tamA	methionine sulfoxide reductase A/translocation and assembly module subunit TamA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,442,349	1	.	G	99.4%	559.8 / ‑3.8	156	intergenic (‑173/‑33)	msrA/tamA	methionine sulfoxide reductase A/translocation and assembly module subunit TamA
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (86/69); total (86/70)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 165 total aligned reads displayed.

TAAGGTTGTAACAAGAGGCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCA‑GGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTACAGGTCGAGGGGTTATCGGGACAGCTG  >  NC_000913/4442210‑4442486

      tGTAACAAGAGGCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCA‑gggggc                                                                                                                                     >  1:965377/1‑138 (MQ=255)

        tAACAAGAGGCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCA‑gggggcag                                                                                                                                   >  1:394518/1‑136 (MQ=255)

            aaGAGGCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGaaa                                                                                                                                <  1:1736306/140‑1 (MQ=255)

            aaGAGGCGTACTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGaaa                                                                                                                                <  1:1653200/140‑1 (MQ=255)

             agagGCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGaaaa                                                                                                                               >  2:1614907/1‑140 (MQ=255)

              gagGCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGaaaaa                                                                                                                              >  1:709206/1‑140 (MQ=255)

              gagGCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGaaaaa                                                                                                                              >  1:1465575/1‑140 (MQ=255)

              gagGCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGaaaaa                                                                                                                              >  1:1456275/1‑140 (MQ=255)

              gagGCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGaaaaa                                                                                                                              >  2:2171773/1‑140 (MQ=255)

              gagGCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGaaaaa                                                                                                                              >  2:1653568/1‑140 (MQ=255)

              gagGCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCA‑ggggg                                                                                                                                      >  1:979748/1‑130 (MQ=255)

               agGTGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGaaaaaa                                                                                                                             >  2:633387/1‑140 (MQ=255)

               agGCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGaaaaaa                                                                                                                             >  2:327767/1‑140 (MQ=255)

               agGCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCA‑gggggc                                                                                                                                     <  1:261667/131‑3 (MQ=255)

               agGCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCA‑gggggc                                                                                                                                     >  2:261667/1‑129 (MQ=255)

                 gCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAgg                                                                                                                           >  2:501950/1‑140 (MQ=255)

                  cGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGaaaaaggat                                                                                                                          >  2:1261877/1‑136 (MQ=255)

                  cGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGa                                                                                                                          >  2:1410077/1‑140 (MQ=255)

                  cGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGa                                                                                                                          >  1:1110275/1‑140 (MQ=255)

                    tATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGata                                                                                                                        >  1:1168255/1‑140 (MQ=255)

                    tATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGata                                                                                                                        >  1:1903276/1‑140 (MQ=255)

                      tttGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATAtt                                                                                                                      >  2:175085/1‑140 (MQ=255)

                      tttGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATAtt                                                                                                                      <  2:947998/140‑1 (MQ=255)

                      tttGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATAtt                                                                                                                      >  1:2094214/1‑140 (MQ=255)

                       ttGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTc                                                                                                                     <  2:720205/140‑1 (MQ=255)

                           acacaATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGga                                                                                                                 >  2:361713/1‑140 (MQ=255)

                              caATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGaa                                                                                                              >  1:153866/1‑140 (MQ=255)

                               aaTAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGaaa                                                                                                             >  2:918603/1‑140 (MQ=255)

                                  aaCCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAAtg                                                                                                          <  1:1931162/140‑1 (MQ=255)

                                    ccaccGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAAtgtg                                                                                                        >  1:2034873/1‑140 (MQ=255)

                                    ccaccGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAAtgtg                                                                                                        <  2:394518/140‑1 (MQ=255)

                                      accGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTgcg                                                                                                      <  2:1465575/140‑1 (MQ=255)

                                      accGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTgcg                                                                                                      <  1:1402522/140‑1 (MQ=255)

                                       ccGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTgcgc                                                                                                     >  2:1423635/1‑140 (MQ=255)

                                         gTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCta                                                                                                   >  1:1642392/1‑140 (MQ=255)

                                         gTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCta                                                                                                   <  1:1416272/140‑1 (MQ=255)

                                         gTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCta                                                                                                   >  2:475918/1‑140 (MQ=255)

                                          tGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCtat                                                                                                  >  2:858379/1‑140 (MQ=255)

                                           gAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCtata                                                                                                 >  1:884360/1‑140 (MQ=255)

                                              tAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATcc                                                                                              <  1:19782/140‑1 (MQ=255)

                                                ggTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGa                                                                                            <  1:207452/140‑1 (MQ=255)

                                                   cTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAg                                                                                         <  1:1614907/140‑1 (MQ=255)

                                                    tAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGt                                                                                        >  1:22795/1‑140 (MQ=255)

                                                       aGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTAt                                                                                     <  2:153866/140‑1 (MQ=255)

                                                        gTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATg                                                                                    <  2:2085914/140‑1 (MQ=255)

                                                        gTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATg                                                                                    <  2:2094214/140‑1 (MQ=255)

                                                        gTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATg                                                                                    <  2:22795/140‑1 (MQ=255)

                                                        gTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATg                                                                                    <  2:1354308/140‑1 (MQ=255)

                                                        gTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATg                                                                                    <  1:1688565/140‑1 (MQ=255)

                                                           aaaCATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATg                                                                                    <  1:588806/137‑1 (MQ=255)

                                                           aaaCATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCtg                                                                                 <  2:1866964/140‑1 (MQ=255)

                                                           aaaCATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCtg                                                                                 >  2:206934/1‑140 (MQ=255)

                                                           aaaCATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCtg                                                                                 >  1:526214/1‑140 (MQ=255)

                                                           aaaCATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCtg                                                                                 >  2:1393656/1‑140 (MQ=255)

                                                           aaaCATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCtg                                                                                 >  1:2267722/1‑140 (MQ=255)

                                                            aaCATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCtgt                                                                                >  2:2346024/1‑140 (MQ=255)

                                                            aaCATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCtgt                                                                                >  2:97388/1‑140 (MQ=255)

                                                            aaCATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCtgt                                                                                >  2:1504180/1‑140 (MQ=255)

                                                            aaCATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCATTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCtgt                                                                                >  2:1155350/1‑140 (MQ=255)

                                                             aCATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCtgtg                                                                               <  2:2034873/140‑1 (MQ=255)

                                                                 aaGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAg                                                                           >  2:497194/1‑140 (MQ=255)

                                                                 aaGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAg                                                                           >  2:1943038/1‑140 (MQ=255)

                                                                   gAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGTGCTATATCCGACAGTTATGCTGTGTAAGCt                                                                         >  2:1486664/1‑140 (MQ=255)

                                                                   gAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCt                                                                         <  1:665315/140‑1 (MQ=255)

                                                                           tGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGc                                                                 <  1:538073/140‑1 (MQ=255)

                                                                               gTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTaa                                                             >  1:101376/1‑140 (MQ=255)

                                                                                 ttAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGG‑AAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCg                                                          >  1:742582/1‑140 (MQ=255)

                                                                                        gCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTg                                                    >  1:2036334/1‑140 (MQ=255)

                                                                                          tttttAATCCATATTACTGGATTTTTGTTAAGCCATTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGcc                                                  <  1:1155350/140‑1 (MQ=255)

                                                                                            tttAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGt                                                <  1:783686/140‑1 (MQ=255)

                                                                                              tAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTcg                                              >  1:1768432/1‑140 (MQ=255)

                                                                                                   cATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCcgcg                                         <  2:2036334/140‑1 (MQ=255)

                                                                                                     tatTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGaa                                       <  2:706699/140‑1 (MQ=255)

                                                                                                     tatTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGaa                                       <  1:1393656/140‑1 (MQ=255)

                                                                                                     tatTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGaa                                       <  2:760685/140‑1 (MQ=255)

                                                                                                     tatTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGaa                                       <  2:2374246/140‑1 (MQ=255)

                                                                                                           tGGATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAAcgtccg                                 >  1:2387761/1‑140 (MQ=255)

                                                                                                             gATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAAcgtccgtc                               >  2:962749/1‑140 (MQ=255)

                                                                                                             gATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAAcgtccgtc                               >  2:1619904/1‑140 (MQ=255)

                                                                                                             gATTTTTGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAAcgtccgtc                               >  1:2221189/1‑140 (MQ=255)

                                                                                                               tttttGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGaa                                       <  1:638717/130‑1 (MQ=255)

                                                                                                               tttttGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGaa                                       >  2:638717/1‑130 (MQ=255)

                                                                                                               tttttGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTa                             >  2:2253052/1‑140 (MQ=255)

                                                                                                                ttttGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTAc                            <  1:2346024/140‑1 (MQ=255)

                                                                                                                ttttGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTAc                            <  2:810048/140‑1 (MQ=255)

                                                                                                                ttttGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTAc                            <  1:490151/140‑1 (MQ=255)

                                                                                                                  ttGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTAc                            >  1:2137610/1‑138 (MQ=255)

                                                                                                                  ttGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTACAg                          >  2:1053566/1‑140 (MQ=255)

                                                                                                                  ttGTTAAGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTACAg                          >  2:226732/1‑140 (MQ=255)

                                                                                                                       aaGCCGTTTAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTACAGGTCGa                     >  2:169179/1‑140 (MQ=255)

                                                                                                                         gCCGTTTAACGGCGTTCCAGGGGGCAGG‑AAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTACAGGTCGAggg                  <  2:742582/140‑1 (MQ=255)

                                                                                                                           cGTTAAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTACAGGTCGAggg                  <  1:1842986/139‑1 (MQ=255)

                                                                                                                             tttAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTACAGGTCGAGGGGtt               >  2:570630/1‑140 (MQ=255)

                                                                                                                               tAACGGCGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTACAGGTCGAGGGGTTAt             <  1:3370/140‑1 (MQ=255)

                                                                                                                                     cGTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTACAGGTCGAGGGGTTATCGGGAc       >  1:1258623/1‑140 (MQ=255)

                                                                                                                                      gTTCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTACAGGTCGAGGGGTTATCGGGACa      >  1:1691809/1‑140 (MQ=255)

                                                                                                                                       ttCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTACAGGTCg                      <  2:1904669/123‑1 (MQ=255)

                                                                                                                                       ttCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTACAGGTCGAGGGGTTATCGGGAc       >  2:421217/1‑138 (MQ=255)

                                                                                                                                       ttCCAGGGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTACAGGTCGAGGGGTTATCGGGAc       <  1:421217/138‑1 (MQ=255)

                                                                                                                                         ccA‑GGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTACAGGTCGAGGGGTTATCGGGACAGCTg  <  1:894860/140‑1 (MQ=255)

TAAGGTTGTAACAAGAGGCGTATTTGCACACAATAACCACCGTGAATAGGTCTAAAGTAAAACATAAGAAATATTTGGGGTTTAGTCTGCTTTTTAATCCATATTACTGGATTTTTGTTAAGCCGTTTAACGGCGTTCCA‑GGGGCAGGAAAAAAGGATATTCAGGAGAAAATGTGCGCTATATCCGACAGTTATGCTGTGTAAGCTTACTCTGCTTAAGCGGATCTGCCGTCGCCGCGAACGTCCGTCTACAGGTCGAGGGGTTATCGGGACAGCTG  >  NC_000913/4442210‑4442486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A