Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,215	(G)_8→9	noncoding (72/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,207	1	.	G	89.9%	332.9 / 28.5	109	noncoding (80/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (6/5); new base G (47/51); total (53/56)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 134 total aligned reads displayed.

TAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCC  >  NC_000913/4606069‑4606341

     aTAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑gggggg                                                                                                                                   <  2:1971148/140‑1 (MQ=255)

     aTAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑gggggg                                                                                                                                   <  2:1713836/140‑1 (MQ=255)

      tAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑ggggggg                                                                                                                                  >  1:259262/1‑140 (MQ=255)

      tAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑ggggggg                                                                                                                                  >  1:642051/1‑140 (MQ=255)

      tAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑ggggggg                                                                                                                                  >  1:650296/1‑140 (MQ=255)

         ttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGGGC‑GA‑‑ggggggggga                                                                                                                               >  2:247488/1‑138 (MQ=255)

         ttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTACGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑ggggggggga                                                                                                                               >  1:146926/1‑138 (MQ=255)

              gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGa                                                                                                                          >  2:1841529/1‑140 (MQ=255)

              gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGa                                                                                                                          >  1:495450/1‑140 (MQ=255)

              gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGa                                                                                                                          >  2:976146/1‑140 (MQ=255)

               cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGaa                                                                                                                         >  2:1319450/1‑140 (MQ=255)

                 aGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAAcc                                                                                                                       >  2:946587/1‑140 (MQ=255)

                    ggggggACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAAccccc                                                                                                                    <  2:1859382/140‑1 (MQ=255)

                    ggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAAccccc                                                                                                                    <  1:1319450/140‑1 (MQ=255)

                     gggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGGG‑ACTTGAAccccc                                                                                                                    <  1:1774763/140‑1 (MQ=255)

                       gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACg                                                                                                                 <  1:786901/140‑1 (MQ=255)

                       gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACg                                                                                                                 <  2:423721/140‑1 (MQ=255)

                          aCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCGCCTACGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTcc                                                                                                              <  2:146926/140‑1 (MQ=255)

                           cTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCg                                                                                                             <  1:1078652/140‑1 (MQ=255)

                            ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTa                                                                                                           >  2:2371808/1‑140 (MQ=255)

                               aaCCCCCACGTCCGTAAGGACACTAACGCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAGCCCCCACGTCCGTAAg                                                                                                         <  1:1116215/140‑1 (MQ=255)

                               aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAg                                                                                                         <  1:1841529/140‑1 (MQ=255)

                               aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAg                                                                                                         <  1:75922/140‑1 (MQ=255)

                               aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAg                                                                                                         <  2:2245852/140‑1 (MQ=255)

                                aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGa                                                                                                        <  2:2256743/140‑1 (MQ=255)

                                aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGa                                                                                                        <  1:765002/140‑1 (MQ=255)

                                      aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTa                                                                                                  <  2:1529263/140‑1 (MQ=255)

                                       cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                 <  2:495450/140‑1 (MQ=255)

                                       cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                 <  1:615774/140‑1 (MQ=255)

                                        gTCCGGAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCATTTGGTGC‑GAGGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                 <  2:1066326/140‑1 (MQ=37)

                                          ccGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAacac                                                                                              <  2:1044237/140‑1 (MQ=255)

                                           cGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                             <  1:1961294/140‑1 (MQ=255)

                                            gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                             >  1:2314671/1‑140 (MQ=255)

                                            gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                 >  2:1157509/1‑135 (MQ=255)

                                             tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                 >  1:946662/1‑133 (MQ=255)

                                             tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                 <  2:946662/133‑1 (MQ=255)

                                                  acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                                                                      >  2:816628/1‑140 (MQ=255)

                                                   cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                                     >  2:562197/1‑140 (MQ=255)

                                                   cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                                     <  2:650296/140‑1 (MQ=255)

                                                   cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                                     <  1:1253417/140‑1 (MQ=255)

                                                   cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                                     >  2:1397056/1‑140 (MQ=255)

                                                    acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                                     <  2:2314671/140‑1 (MQ=255)

                                                    acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGGGC‑GA‑‑GGGGGGGGCACTTCAACCCCCACCGCCGAAAGAACACTAACACCTGAAGCTAg                                                                                    >  2:459793/1‑140 (MQ=37)

                                                     cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                                  >  1:165435/1‑140 (MQ=255)

                                                      tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc                                                                                 >  1:600980/1‑140 (MQ=255)

                                                              gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                          >  1:2098336/1‑140 (MQ=255)

                                                              gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                          >  2:975764/1‑140 (MQ=255)

                                                              gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGAGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                          >  2:55537/1‑140 (MQ=255)

                                                                aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                                                        >  1:240034/1‑140 (MQ=255)

                                                                aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                       >  2:616560/1‑140 (MQ=255)

                                                                    aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACAAATTc                                                                     >  2:1353130/1‑140 (MQ=255)

                                                                    aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                   <  2:165435/140‑1 (MQ=255)

                                                                      cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑GCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  <  2:2167021/140‑1 (MQ=255)

                                                                      cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  <  1:1397056/140‑1 (MQ=255)

                                                                      cgcgTCAACCAAAACCGGCGCCTACGCACAGTCATCTTACTATTATTGATAGCACCGCGATTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  <  2:195721/140‑1 (MQ=17)

                                                                           cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                                                             >  1:2275925/1‑140 (MQ=255)

                                                                                aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                        <  1:373503/140‑1 (MQ=255)

                                                                                 aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                                                       <  1:946587/140‑1 (MQ=255)

                                                                                     cggcgacTTCGCACAATCAACTTACTTTTTTTGATATCACCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                   <  2:2160283/134‑1 (MQ=18)

                                                                                     cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                   <  2:1348364/140‑1 (MQ=255)

                                                                                     cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                   <  1:55537/140‑1 (MQ=255)

                                                                                     cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                   >  1:1271406/1‑140 (MQ=255)

                                                                                     cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                   >  1:1740356/1‑140 (MQ=255)

                                                                                      gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACGCTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                        >  1:1010705/1‑134 (MQ=255)

                                                                                      gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACGCTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                        <  2:1010705/134‑1 (MQ=255)

                                                                                      gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                                  >  2:783512/1‑140 (MQ=255)

                                                                                       ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                              <  2:1798995/128‑1 (MQ=255)

                                                                                       ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                              >  1:1798995/1‑128 (MQ=255)

                                                                                       ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                 <  2:2098336/140‑1 (MQ=255)

                                                                                        cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCGACGTCCGAAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCGACCTTCGCCCAggacga                                                >  2:1676009/1‑135 (MQ=21)

                                                                                        cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa                                                >  1:676659/1‑140 (MQ=255)

                                                                                        cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                               <  2:600980/140‑1 (MQ=255)

                                                                                         tccTTCGAACAGTCATCTTACTTTTTTTGATATCACCGCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                               <  2:1466328/139‑1 (MQ=18)

                                                                                         gccTTAGCACAGACGACTTACTTTTTTTGAAATCAACGCGATTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                               <  2:2261222/139‑1 (MQ=17)

                                                                                         aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                               <  1:783512/140‑1 (MQ=255)

                                                                                               gCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCTACCTTCGCCCAGTGCGAGCAatat                                         <  1:2304460/140‑1 (MQ=255)

                                                                                                   aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                     <  1:975764/140‑1 (MQ=255)

                                                                                                   aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                     <  1:816628/140‑1 (MQ=255)

                                                                                                     tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                                   >  2:1740532/1‑140 (MQ=255)

                                                                                                     tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                                   >  1:537636/1‑140 (MQ=255)

                                                                                                     tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGCGCGAGCTATATCAACGt                                   >  1:363752/1‑140 (MQ=255)

                                                                                                        tCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGt                                >  1:725342/1‑140 (MQ=255)

                                                                                                        tCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGCGAGCACTATCAACGAGGt                                >  2:271709/1‑140 (MQ=21)

                                                                                                           tACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt                             <  1:1589201/140‑1 (MQ=255)

                                                                                                               ttttttGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                                   >  1:195643/1‑130 (MQ=255)

                                                                                                               ttttttGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                                   <  2:195643/130‑1 (MQ=255)

                                                                                                               ttttttGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTGGt                         >  2:300627/1‑140 (MQ=255)

                                                                                                               ttttatGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGCGAGCAATATCAACGTGGTTTTTgca                         >  2:942193/1‑138 (MQ=21)

                                                                                                                  tttGATAACGCCTGGTTGGGTGC‑GAG‑GGGGGGGG‑ACTCGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCg                      <  2:1145614/140‑1 (MQ=21)

                                                                                                                   ttGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                     <  1:247488/140‑1 (MQ=255)

                                                                                                                   ttGATATCGCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACAAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                     <  2:1773174/140‑1 (MQ=255)

                                                                                                                   ttGATATCACCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                     <  2:846950/140‑1 (MQ=255)

                                                                                                                        atCGCCTCGTTTGGTGC‑GAGGGGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTGGTGCGAgggg                 >  1:61423/1‑140 (MQ=255)

                                                                                                                        atCGACTCGTTTGGTGCGGG‑‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                <  1:423579/140‑1 (MQ=255)

                                                                                                                           gCCTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggggg             <  2:725342/140‑1 (MQ=255)

                                                                                                                            ccTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggggc            >  2:1271011/1‑139 (MQ=255)

                                                                                                                             cTCGTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAc           <  2:1271406/140‑1 (MQ=255)

                                                                                                                               ggTTTGGTGC‑GAG‑GGGGGGGG‑ACTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACtt         <  2:286346/139‑1 (MQ=255)

                                                                                                                                 tttGGTGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGa       >  1:1807312/1‑140 (MQ=255)

                                                                                                                                      tGC‑GAG‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc  <  2:1740356/140‑1 (MQ=255)

TAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCC  >  NC_000913/4606069‑4606341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A