Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,215	(G)_8→9	noncoding (72/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,207	1	.	G	98.1%	175.2 / ‑3.6	52	noncoding (80/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (26/25); total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑GG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACG  >  NC_000913/4606074‑4606345

aTAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑gg‑gg‑g                                                                                                                                        <  1:704318/140‑1 (MQ=255)

   aTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑gg‑gg‑gg‑gg                                                                                                                                    >  2:1234656/1‑140 (MQ=255)

      ggTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑gg‑gg‑gg‑gggac                                                                                                                                 <  1:238865/140‑4 (MQ=255)

         gcgagggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCTTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTg                                                                                                                               >  2:690556/6‑140 (MQ=21)

         gcgagggggggggACTTGAACCCCCACATCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCGATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTg                                                                                                                               <  1:537412/135‑1 (MQ=21)

            agggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAAc                                                                                                                            >  1:924911/3‑140 (MQ=255)

                 gggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCa                                                                                                                       <  1:770379/140‑1 (MQ=255)

                 gggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑gg‑gg‑gg                                                                                                                                       <  2:560352/124‑1 (MQ=255)

                 gggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑gg‑gg‑gg                                                                                                                                       >  1:560352/1‑124 (MQ=255)

                 gggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGAACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCa                                                                                                                       <  2:423181/140‑1 (MQ=255)

                      aCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTcc                                                                                                                  >  1:624640/1‑140 (MQ=255)

                         tGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACg                                                                                                                     <  2:554243/134‑1 (MQ=255)

                         tGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACg                                                                                                                     >  1:554243/1‑134 (MQ=255)

                          gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAAccccc                                                                                                                        >  1:287974/1‑130 (MQ=255)

                          gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAAccccc                                                                                                                        <  2:287974/130‑1 (MQ=255)

                                 cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTCATCTTACTTTCTTTGATGTCGCCGCGTCTGGTGC‑GAGGG‑GG‑GG‑GGCCCTAAACCCCCACGGCCGCAAAAACACt                                                                                                       >  2:28491/1‑140 (MQ=18)

                                   cGTCCGTAAGGACACTAACACCTGAAGCTGGTGCGTCTACCAATTCCGCCACCTTCGCACAGGCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                     <  2:629616/140‑1 (MQ=37)

                                       cGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                                 <  1:244948/140‑1 (MQ=255)

                                         tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                                                               >  1:162161/1‑140 (MQ=255)

                                         tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                                                               >  1:332171/1‑140 (MQ=255)

                                            ggACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGagg                                                                                            <  2:924911/140‑3 (MQ=255)

                                              acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                                                                <  2:141368/134‑1 (MQ=255)

                                              acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                                                                >  1:141368/1‑134 (MQ=255)

                                              acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                                                                          >  2:72893/1‑140 (MQ=255)

                                                cttAACTCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTCTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                                        <  2:1121381/138‑1 (MQ=38)

                                                    acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                                    >  1:129347/1‑140 (MQ=255)

                                                          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGCAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                              >  1:490036/1‑140 (MQ=255)

                                                                  cgcgTCTACCAATTCCGCCACCTTCGCCCAGTCATCTTACTTTTTTTGATATCGCCTGGTTTGGGGG‑GG‑GG‑GG‑GGTGGAATTCAACCCCCACGCTGGTAACGACACCAACAGCTGATGCTAGCGCGGTTACCAATTCCGc                                                                      >  2:282176/1‑140 (MQ=19)

                                                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                             <  2:725339/133‑1 (MQ=255)

                                                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                             >  1:725339/1‑133 (MQ=255)

                                                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                      <  2:129347/140‑1 (MQ=255)

                                                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAA‑CCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                     >  1:258787/1‑140 (MQ=255)

                                                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAA‑CCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                            <  2:258787/140‑1 (MQ=255)

                                                                            aaTTCCGCCACCTTCGCCCAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                            <  1:932816/140‑1 (MQ=255)

                                                                            aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGGG‑GG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                            <  2:162161/140‑1 (MQ=255)

                                                                             aTTTCGCCAACTTCGCACAGGCAGCTTATATTTTTGGATATCGCCTTGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                                                           <  2:875762/140‑1 (MQ=17)

                                                                                     aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                   >  1:525115/1‑140 (MQ=255)

                                                                                        ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAACCTAGCGCGTCTACCAATTCCGCCACCTTCGCCTAGCGCGAGCaa                                                >  2:864473/1‑140 (MQ=21)

                                                                                               aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                         <  2:261255/140‑1 (MQ=255)

                                                                                                gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                   <  2:98338/129‑1 (MQ=255)

                                                                                                gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                   >  1:98338/1‑129 (MQ=255)

                                                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                                       >  1:1207475/1‑140 (MQ=255)

                                                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                                       >  2:850162/1‑140 (MQ=255)

                                                                                                  cATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACAAACACCTCAAGCTAACGCGCCTACCAATTCCCCCACCCTCGCCCAGTGCCAGCAATATCAACggg                                      >  2:774293/1‑138 (MQ=21)

                                                                                                    tCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGt                                    >  2:482993/1‑140 (MQ=255)

                                                                                                           ttttttGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAatat                                             <  2:869604/124‑1 (MQ=255)

                                                                                                           ttttttGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAatat                                             >  1:869604/1‑124 (MQ=255)

                                                                                                           ttttttGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                             >  2:274983/1‑140 (MQ=255)

                                                                                                           ttttttGATATAGCCTCGTTTGGTGC‑GA‑GG‑GGTGG‑GGACTTGTACCCCCGCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                             <  1:282176/140‑1 (MQ=21)

                                                                                                             ttttGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGc                           <  2:624640/140‑1 (MQ=255)

                                                                                                               ttGATATCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                         >  1:489575/1‑140 (MQ=255)

                                                                                                                  atatCGCCTCGTTCGGTAG‑GA‑GGCGG‑GG‑GCAGCTGAACCCCCACGTCCGAAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggg                      <  2:490036/140‑1 (MQ=21)

                                                                                                                   tatCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGCCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTGGAGCGAgggg                     >  2:997561/1‑140 (MQ=21)

                                                                                                                   tatCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggg                     <  2:489575/140‑1 (MQ=255)

                                                                                                                    atCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                    <  2:525115/140‑1 (MQ=255)

                                                                                                                    atCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                    <  1:690556/140‑1 (MQ=255)

                                                                                                                     tCGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggg                   <  1:1234656/140‑1 (MQ=255)

                                                                                                                      cGCCTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggg                  >  1:501328/1‑140 (MQ=255)

                                                                                                                        ccTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggggac                >  2:817733/1‑138 (MQ=255)

                                                                                                                        ccTCGTTTGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggggac                <  1:817733/140‑3 (MQ=255)

                                                                                                                             tttGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGa           >  2:504057/1‑139 (MQ=255)

                                                                                                                             tttGGTGC‑GAGGG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGa           <  1:504057/139‑1 (MQ=255)

                                                                                                                                     ggA‑GG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTATACCAATTCCGCCACCTTCGCCCAGTGCGAGTAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACg  <  2:708063/139‑1 (MQ=255)

ATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑GG‑GG‑GG‑GGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACG  >  NC_000913/4606074‑4606345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A