Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F28 I1 R1
|
159 |
82.1 |
2830106 |
99.3% |
2810295 |
139.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,624,072 |
C→T |
intergenic (‑222/+33) |
dgcZ ← / ← ydeI |
diguanylate cyclase DgcZ/BOF family protein YdeI |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,624,072 | 0 | C | T | 98.4%
| 214.4
/ NA
| 62 | intergenic (‑222/+33) | dgcZ/ydeI | diguanylate cyclase DgcZ/BOF family protein YdeI |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (37/24); minor base A (1/0); total (38/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTTATGAGTCATCTCTGAGATAACATTGTGATTTAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGCCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTAATGTTGATCATCTGGTCCGGCTGAACTTCTCGTCCATC > NC_000913/1623937‑1624206
|
gTTATGAGTCATCTCTGAGATAACATTGTGATTTAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCag < 1:911003/140‑1 (MQ=255)
aGTCATCTCTGAGATAACATTGTGATTTAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTc < 2:1103413/140‑1 (MQ=255)
gTCATCTCTGAGATAACATTGTGATTTAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCa < 1:1119645/140‑1 (MQ=255)
tCATCTCTGAGATAACATTGTGATTTAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCag < 2:228464/132‑1 (MQ=255)
tCATCTCTGAGATAACATTGTGATTTAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCag > 1:228464/1‑132 (MQ=255)
tctcTGAGATAACATTGTGATTTAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGcc > 2:968505/1‑140 (MQ=255)
tctcTGAGATAACATTGTGATTTAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGcc > 1:292698/1‑140 (MQ=255)
tctGAGATAACATTGTGATTTAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCAt < 1:1101598/140‑1 (MQ=255)
agaTAACATTGTGATTTAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAgg > 2:1063678/1‑140 (MQ=255)
cATTGTGATTTAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAatt > 1:382291/1‑140 (MQ=255)
ttGTGATTTAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAattta > 2:899736/1‑140 (MQ=255)
gATTTAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCAt < 2:267022/124‑1 (MQ=255)
gATTTAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCAt > 1:267022/1‑124 (MQ=255)
tAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAAtttatttttatt > 2:737060/1‑140 (MQ=255)
tAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTATAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGACCAGTAGTTCAGGCCATCAGGCTAAtttatttttatt > 2:360923/1‑140 (MQ=255)
aaaaCAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAACGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATttatttttattt < 2:1227967/140‑1 (MQ=255)
aaCAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCt > 1:1370746/1‑140 (MQ=255)
aaaaTCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCa < 2:1117163/140‑1 (MQ=255)
aaTCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCaaa > 1:612488/1‑140 (MQ=255)
gtTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGAcgg > 2:20700/1‑140 (MQ=255)
gtTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGAcgg > 2:869239/1‑140 (MQ=255)
tttAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGAcggc > 2:1044924/1‑140 (MQ=255)
ttAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTGATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGAcggcc > 2:1303594/1‑140 (MQ=255)
tAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGAcggccg < 2:382291/140‑1 (MQ=255)
tGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGgcgcgc < 1:869239/140‑1 (MQ=255)
tGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGgcgcgc < 2:463597/140‑1 (MQ=255)
tAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGgcgcg > 1:657267/1‑137 (MQ=255)
tAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGgcgcg < 2:657267/137‑1 (MQ=255)
tAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCtt > 2:300275/1‑140 (MQ=255)
aTTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTc < 2:1007699/140‑1 (MQ=255)
tACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGgcg < 2:240982/130‑1 (MQ=255)
tACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGgcg > 1:240982/1‑130 (MQ=255)
ctctGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATc > 2:692223/1‑140 (MQ=255)
ctGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCa > 2:328503/1‑140 (MQ=255)
gCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCtt > 2:1019099/1‑128 (MQ=255)
gCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCtt < 1:1019099/128‑1 (MQ=255)
tATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGc < 1:1063678/140‑1 (MQ=255)
tCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCa > 2:1382352/1‑140 (MQ=255)
tCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCa < 1:359651/140‑1 (MQ=255)
tCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCa < 1:899736/140‑1 (MQ=255)
tCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCa < 1:692223/140‑1 (MQ=255)
gTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACt > 1:1143602/1‑140 (MQ=255)
aaataaaAGGATGACAAATAGCATAACCCAATTCCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTaa < 2:1318203/140‑1 (MQ=255)
aataaaAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCa < 1:975542/135‑1 (MQ=255)
aataaaAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCa > 2:975542/1‑135 (MQ=255)
aataaaAGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTAAt > 2:96048/1‑140 (MQ=255)
aaGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTaa > 1:466372/1‑134 (MQ=255)
aaGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTaa < 2:466372/134‑1 (MQ=255)
aaGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTAATGTTGa > 1:546385/1‑140 (MQ=255)
aaGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTAATGTTGa > 1:944316/1‑140 (MQ=255)
aaGGATGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTAATGTTGa < 2:799496/140‑1 (MQ=255)
tGACAAATAGCATAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCATACTGCCACTAATGTTGatcatc > 1:1145118/1‑140 (MQ=255)
tAACCCAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTAATGTTGATCATCTGGTCCGGCTGa > 1:297650/1‑140 (MQ=255)
cccAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTAATGTTGATCATCTGGTCCGGCTGAACt > 2:662816/1‑140 (MQ=255)
cccAATACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTAATGTTGATCATCTGGTCCGGCTGAACt > 2:1233476/1‑140 (MQ=255)
tACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGATTGCCACTAATGTTGATCATCTGGTCCGGCTGAACTTCTCg > 1:704388/1‑140 (MQ=255)
tACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTAATGTTGATCATCTGGTCCGGCTGAACTTCTCg < 1:20700/140‑1 (MQ=255)
tACCCTAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTAATGTTGATCATCTGGTCCGGCTGAACTTCTCg > 1:222903/1‑140 (MQ=255)
tAATGGTTCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTAATGTTGATCATCTGGTCCGGCTGAACTTCTCGTCCAt > 1:973351/1‑140 (MQ=255)
tAATGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTAATGTTGATCATCTGGTCCGGCTGAACTTCTCGTCCAt > 2:26296/1‑140 (MQ=255)
aaTGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTAATGTTGATCATCTGGTCCGGCTGAACTTCTCGTCCATc > 1:1402597/1‑140 (MQ=255)
aaTGGTCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTAATGTTGATCATCTGGTCCGGCTGAACTTCTCGTCCATc > 1:380994/1‑140 (MQ=255)
|
GTTATGAGTCATCTCTGAGATAACATTGTGATTTAAAACAAAATCAGCGGATAAAAAAGTGTTTAATTCTGTAAATTACCTCTGCATTATCGTAAATAAAAGGATGACAAATAGCATAACCCAATACCCTAATGGCCCAGTAGTTCAGGCCATCAGGCTAATTTATTTTTATTTCTGCAAATGAGTGACCCGAACGACGGCCGGCGCGCTTTTCTTATCCAGACTGCCACTAATGTTGATCATCTGGTCCGGCTGAACTTCTCGTCCATC > NC_000913/1623937‑1624206
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
